PMCC PMCC

Search tips
Search criteria

Advanced
Results 1-9 (9)
 

Clipboard (0)
None

Select a Filter Below

Journals
Authors
more »
Year of Publication
Document Types
1.  Effects of HLEC on the secreted proteins of epithelial ovarian cancer cells prone to metastasize to lymph nodes 
Cancer Biology & Medicine  2013;10(4):221-226.
Objective
To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer (EOC) cells (SKOV3-PM4) with directional highly lymphatic metastasis.
Methods
Supernatants of four groups of cultured cells, namely, SKOV3 (A), SKOV3+HLEC (B), SKOV3-PM4 (C), SKOV3-PM4+HLEC (D), were collected, and their proteins were detected by antibody arrays and iTRAQ-2D-LC-MALDI-TOF/TOF/MS. Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA.
Results
Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C (compared with group A), whereas IGFBP7 and SPARC were downregulated in group D (compared with group C). Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other. Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers, benign tumors, and control groups. Two proteins were upegulated in the first group. VEGFA in the control group was downregulated. For IGFBP, upregulation in the control group and down-regulation in the first group were also observed.
Conclusion
The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors. The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.
doi:10.7497/j.issn.2095-3941.2013.04.006
PMCID: PMC3860345  PMID: 24349832
Ovarian cancer; tumor microenvironment; lymphatic metastasis; human lymphatic capillary endothelial cells; secreted proteins
2.  3-[(1-Bromo­naphthalen-2-yl)meth­oxy]-5,5-di­methyl­cyclo­hex-2-enone 
In the title compound, C19H19BrO2, the cyclo­hexenone ring adopts an envelope conformation with the C atom bearing the methyl substituents as the flap. In the crystal, weak π–π stacking is observed between parallel aromatic rings of adjacent mol­ecules, the centroid–centroid distance being 3.694 (6) Å. The entire bromonaphthylmethyl unit is disordered over two orientations, with a site-occupancy ratio of 0.5214 (19):0.4786 (19).
doi:10.1107/S160053681301026X
PMCID: PMC3648284  PMID: 23723904
3.  1-[5-(2-Chloro­phen­yl)-5-hy­droxy-3-methyl-4,5-dihydro-1H-pyrazol-1-yl]­ethanone 
The title compound, C12H13ClN2O2, crystallizes with two independent but very similar mol­ecules (A and B) in the asymmetric unit. The pyrazole ring in each mol­ecule has an envelope conformation. The dihedral angle between the pyrazole ring mean plane and the benzene ring is 86.07 (14)° in A and 85.99 (14)° in B. In the crystal, the A and B mol­ecules are linked via a pair of O—H⋯O hydrogen bonds, forming dimers. These dimers are further linked via C—H⋯O inter­actions to form –A–B–A–B– chains propagating along the c-axis direction.
doi:10.1107/S1600536812007283
PMCID: PMC3297897  PMID: 22412700
4.  [(2R,3S,5R)-3-Acet­oxy-5-(5-formyl-2,4-dioxo-1,2,3,4-tetra­hydro­pyrimidin-1-yl)-2,3,4,5-tetra­hydro­furan-2-yl]methyl acetate 
In the two independent but very similar mol­ecules (A and B) of the title compound, C14H16N2O8, both six-membered pyrimidine rings are nearly planar [maximum deviations = 0.010 (3) Å in A and 0.028 (3) Å in B]. The five-membered furan­ose ring in mol­ecule A adopts an envelope conformation, while the same ring in mol­ecule B has a twisted conformation. In the crystal, the A mol­ecules are linked via a pair of inter­molecular N—H⋯O hydrogen bonds, forming dimers. Each A mol­ecule is further linked to a B mol­ecule via a second N—H⋯O hydrogen bond. There are also a number of C—H⋯·O inter­actions present, leading to the formation of a three-dimensional network.
doi:10.1107/S1600536811041304
PMCID: PMC3247360  PMID: 22219978
5.  2,4-Dioxo-1-(prop-2-yn­yl)-1,2,3,4-tetra­hydro­pyrimidine-5-carbaldehyde 
In the crystal structure of the title compound, C8H6N2O3, the mol­ecules are linked by a pairs of inter­molecular N—H⋯O hydrogen bonds, forming inversion dimers. The aldehyde group is in the same plane as the pyrimidine ring [with a maximum deviation of 0.083 (2) Å for the O atom), and the linear propargyl group [C—C—C = 178.99 (19)°] makes a dihedral angle of 74.36 (13)° with the ring.
doi:10.1107/S1600536811032272
PMCID: PMC3200754  PMID: 22058966
6.  4-(4-Bromo­phen­yl)-4,5,6,7-tetra­hydro-3-methyl-6-oxo-1-phenyl-1H-pyrazolo[3,4-b]pyridine-5-carbonitrile ethanol solvate 
In the structure of the title compound, C20H15BrN4O·C2H6O, the hydrogenated pyridinone ring adopts an envelope conformation. The dihedral angle between the bromo-substituted phenyl ring and the pyrazole ring is 79.6 (1)°, and that between the non-substituted phenyl ring and the pyrazole ring is 51.2 (1)°. In the crystal structure, mol­ecules are linked via inter­molecular N—H⋯O and O—H⋯N hydrogen bonds. A short inter­molecular N⋯Br contact [3.213 (4) Å] is present in the crystal structure.
doi:10.1107/S1600536808028638
PMCID: PMC2959421  PMID: 21201133
7.  6,7-Dihydro-4-(4-methoxy­phen­yl)-3-methyl-6-oxo-1-phenyl-1H-pyrazolo[3,4-b]pyridine-5-carbonitrile 
In the title compound, C21H16N4O2, the dihedral angle between the meth­oxy-substituted benzene ring and the ring system formed by the pyridinone ring and the pyrazole ring is 57.4 (1)°, and that between the unsubstituted phenyl ring and the ring system is 135.6 (1)°. In the crystal structure, mol­ecules are linked together via inter­molecular N—H⋯O hydrogen bonds.
doi:10.1107/S1600536808027852
PMCID: PMC2959259  PMID: 21201100
8.  2-[(5-Amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)(4-chloro­phen­yl)meth­yl]malononitrile 
In the crystal structure of the title compound, C20H16ClN5, the dihedral angle between the pyrazole ring and the phenyl ring is 54.7 (1)° and that between the pyrazole ring and the chloro-substituted phenyl ring is 72.4 (1)°. The methyl H atoms are disordered over two positions with site occupancy factors of ca 0.7 and 0.3. One amino H is disordered equally over two positions. In the crystal structure, the mol­ecules are linked via inter­molecular N—H⋯N hydrogen bonding.
doi:10.1107/S1600536808011562
PMCID: PMC2961285  PMID: 21202390
9.  6-Amino-3-methyl-4-(4-nitro­phen­yl)-1-phenyl­pyrazolo[3,4-b]pyridine-5-carbonitrile 
The title compound, C20H14N6O2, contains four rings. The dihedral angle between the pyridine ring and the pyrazole ring is 1.9 (1)°, i.e. almost coplanar, which gives rise to a conjugated structure. The dihedral angle between the nitro-substituted phenyl ring and the pyridine ring is 76.3 (1)° and that between the pyrazole ring and the non-substituted phenyl ring is 40.5 (1)°. In the crystal structure, symmetry-related mol­ecules are linked by N—H⋯O and C—H⋯N hydrogen bonds.
doi:10.1107/S1600536808006156
PMCID: PMC2961049  PMID: 21202081

Results 1-9 (9)