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International Journal of Nanomedicine (1)
Journal of Assisted Reproduction and Genetics (1)
The Journal of Cell Biology (1)
Xia, Lu (3)
Bré, Marie-Helene (1)
Burnette, Dylan (1)
Ding, Siyang (1)
Duan, Jianming (1)
Feng, Bo (1)
Gaertig, Jacek (1)
Gao, Yan (1)
Gorovsky, Martin A. (1)
Hai, Bing (1)
Levilliers, Nicolette (1)
Li, Chen (1)
Li, Ming-Li (1)
Liu, Yu-Xin (1)
Liu, Zhiyuan (1)
Thazhath, Rupal (1)
Wang, Peizhi (1)
Wu, Jin-Fang (1)
Yu, Rong (1)
Zhang, Xin (1)
Zhou, Jie (1)
Year of Publication
The high incidence of STR D21S1446 homozygosity in Han and She populations living in South Eastern China
Journal of Assisted Reproduction and Genetics
This study investigated the allelic frequency of 4 short terminal repeats (STRs) in Han and She populations from eastern China.
DNA samples from Han (N = 110) and She (N = 110) healthy subjects were amplified using PCR and primers specific for the STRs. D21S11 and D21S1446 (chromosome 21) and D18S865 and D18S535 (chromosome 18). The frequency and homozygocity of different STRs were evaluated.
For both populations, D21S11 and D18S535 were more polymorphic than D21S1446 and D18S865, and the D21S1446 1 allele was very common (48.6% and 55.0% for the Han and She groups, respectively).The Han and She groups significantly differed from one another in respect to the distribution of D21S11, D18S865, and D18S535 allelic frequency (all P -values ≤ 0.48) but had a similar allelic distribution for D21S1446 (P = 0.106). D21S1446 was found to be homozygous about 50% of the time which was significantly greater compared to the other STRs analyzed.
This study found that the Han and She differ genetically at 3 autosomal STRs located on chromosome 18 and 21. The STR D21S1146 was often homozygous within these groups, indicating it would be poor marker for analyzing trisomy within these populations.
Short tandem repeat; Trisomy detection; Allele homozygosity; She group; Genetic variant; Polymerase chain reaction
In vitro and in vivo studies of surface-structured implants for bone formation
International Journal of Nanomedicine
Background and methods
Micronanoscale topologies play an important role in implant osteointegration and determine the success of an implant. We investigated the effect of three different implant surface topologies on osteoblast response and bone regeneration. In this study, implants with nanotubes and micropores were used, and implants with flat surfaces were used as the control group.
Our in vitro studies showed that the nanostructured topologies improved the proliferation, differentiation, and development of the osteoblastic phenotype. Histological analysis further revealed that the nanotopology increased cell aggregation at the implant-tissue interfaces and enhanced bone-forming ability. Pushout testing indicated that the nanostructured topology greatly increased the bone-implant interfacial strength within 4 weeks of implantation.
Nanotopography may improve regeneration of bone tissue and shows promise for dental implant applications.
osteoblast; osteointegration; titanium; nanostructure; microstructure
Polyglycylation of Tubulin Is Essential and Affects Cell Motility and Division in Tetrahymena thermophila
Gorovsky, Martin A.
The Journal of Cell Biology
We analyzed the role of tubulin polyglycylation in Tetrahymena thermophila using in vivo mutagenesis and immunochemical analysis with modification-specific antibodies. Three and five polyglycylation sites were identified at glutamic acids near the COOH termini of α- and β-tubulin, respectively. Mutants lacking all polyglycylation sites on α-tubulin have normal phenotype, whereas similar sites on β-tubulin are essential. A viable mutant with three mutated sites in β-tubulin showed reduced tubulin glycylation, slow growth and motility, and defects in cytokinesis. Cells in which all five polyglycylation sites on β-tubulin were mutated were viable if they were cotransformed with an α-tubulin gene whose COOH terminus was replaced by the wild-type COOH terminus of β-tubulin. In this double mutant, β-tubulin lacked detectable polyglycylation, while the α-β tubulin chimera was hyperglycylated compared with α-tubulin in wild-type cells. Thus, the essential function of polyglycylation of the COOH terminus of β-tubulin can be transferred to α-tubulin, indicating it is the total amount of polyglycylation on both α- and β-tubulin that is essential for survival.
motor proteins; microtubules; cilia; cytoskeleton; motility
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