Storage of RBCs is necessary for an adequate blood supply. However, reports have identified potential negative sequelae of transfusing stored RBCs. An animal model would be useful to investigate the pathophysiology of transfusing stored RBCs. However, it has been reported that storage of rat RBCs in CPDA-1 resulted in an unexpected sudden decline in post-transfusion survival. We developed a mouse model of RBC storage and transfusion to assess survival kinetics of mouse RBCs.
Study Design and Methods
RBCs expressing green fluorescent protein were collected in CPDA-1, filter leukoreduced, adjusted to a 75% hematocrit, and stored at 4°C. At weekly intervals, stored RBCs were transfused into C57BL/6 recipients. RBC survival was measured by flow cytometry and 51Chromium labeling. Phosphatidylserine externalization and CD47 expression was also evaluated.
Mean 24-hour survival of transfused RBCs was 99%, 91%, 64%, 54%, 30%, and 18% following 0, 7, 14, 21, 28, and 35 days of storage, respectively. Stored RBCs showed an initial rapid clearance with subsequent extended survival. Increased surface phosphatidylserine and decreased CD47 expression was also observed.
Mouse RBCs showed a progressive decline in survival, as a function of storage time, unlike the precipitous loss of viability previously reported for rat RBCs. Moreover, changes in the measured surface markers were analogous to trends reported for human RBCs. Together, these findings provide an initial characterization of a novel mouse model of RBC storage with the potential to serve as an experimental platform for studying the pathophysiological consequences of transfusing stored RBCs.