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1.  Subcellular evidence for the involvement of peroxisomes in plant isoprenoid biosynthesis 
Plant Signaling & Behavior  2011;6(12):2044-2046.
The role of peroxisomes in isoprenoid metabolism, especially in plants, has been questioned in several reports. A recent study of Sapir-Mir et al.1 revealed that the two isoforms of isopentenyl diphosphate (IPP) isomerase, catalyzing the isomerisation of IPP to dimethylallyl diphosphate (DMAPP) are found in the peroxisome. In this addendum, we provide additional data describing the peroxisomal localization of 5-phosphomevalonate kinase and mevalonate 5-diphosphate decarboxylase, the last two enzymes of the mevalonic acid pathway leading to IPP.2 This finding was reinforced in our latest report showing that a short isoform of farnesyl diphosphate, using IPP and DMAPP as substrates, is also targeted to the organelle.3 Therefore, the classical sequestration of isoprenoid biosynthesis between plastids and cytosol/ER can be revisited by including the peroxisome as an additional isoprenoid biosynthetic compartment within plant cells.
doi:10.4161/psb.6.12.18173
PMCID: PMC3337203  PMID: 22080790
5-phosphomevalonate kinase; Arabidopsis thaliana; Catharanthus roseus; farnesyl diphosphate synthase; isoprenoid; mevalonate 5-diphosphate decarboxylase; mevalonic acid pathway; peroxisome
2.  Multigene manipulation of photosynthetic carbon assimilation increases CO2 fixation and biomass yield in tobacco 
Journal of Experimental Botany  2015;66(13):4075-4090.
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Multigene manipulation of levels of Calvin cycle enzymes, together with the introduction of a putative cyanobacterial inorganic carbon transporter, results in substantial improvements in biomass yield. This study demonstrates that this approach has the potential to produce crop plants to meet the food requirements of a growing population.
Over the next 40 years it has been estimated that a 50% increase in the yield of grain crops such as wheat and rice will be required to meet the food and fuel demands of the increasing world population. Transgenic tobacco plants have been generated with altered combinations of sedoheptulose-1,7-bisphosphatase, fructose-1,6-bisphosphate aldolase, and the cyanobacterial putative-inorganic carbon transporter B, ictB, of which have all been identified as targets to improve photosynthesis based on empirical studies. It is shown here that increasing the levels of the three proteins individually significantly increases the rate of photosynthetic carbon assimilation, leaf area, and biomass yield. Furthermore, the daily integrated measurements of photosynthesis showed that mature plants fixed between 12–19% more CO2 than the equivalent wild-type plants. Further enhancement of photosynthesis and yield was observed when sedoheptulose-1,7-bisphosphatase, fructose-1,6-bisphosphate aldolase, and ictB were over-expressed together in the same plant. These results demonstrate the potential for the manipulation of photosynthesis, using multigene-stacking approaches, to increase crop yields.
doi:10.1093/jxb/erv204
PMCID: PMC4473996  PMID: 25956882
Biomass; Calvin–Benson cycle; chlorophyll fluorescence imaging; gas exchange; gene stacking.

Results 1-2 (2)