PMCC PMCC

Search tips
Search criteria

Advanced
Results 1-2 (2)
 

Clipboard (0)
None

Select a Filter Below

Journals
Authors
more »
Year of Publication
Document Types
1.  No evidence for a direct role of Helicobacter pylori and Mycoplasma pneumoniae in carotid artery atherosclerosis 
Journal of Clinical Pathology  2006;59(11):1186-1190.
Background
That infections with certain pathogens, by initiating an inflammatory response, may contribute to the development of atherosclerosis is suggested by clinical and experimental evidence.
Aim
To analyse atherosclerotic plaques of the carotid artery, samples of apparently healthy greater saphenous veins and circulating leucocytes from the same individual patients for the presence of Helicobacter pylori and Mycoplasma pneumoniae.
Methods
Samples from 36 patients undergoing carotid endarterectomy for symptomatic carotid artery stenosis were analysed by polymerase chain reaction for the presence of DNA specific for H pylori and M pneumoniae. IgG antibody titres against H pylori and M pneumoniae and plasma levels of soluble E‐selectin, soluble intercellular adhesion molecule‐1 and soluble vascular cell adhesion molecule‐1 were determined.
Results
M pneumoniae‐specific DNA was detected in the atherosclerotic plaques of 13 of 36 (36.1%) patients, in the saphenous veins of 9 of 36 (25%) patients and in the leucocytes of 27 of 36 (75%) patients. No salient association was observed between the presence of M pneumoniae‐specific DNA in leucocytes and atherosclerotic plaques or veins. A marked correlation between the presence of M pneumoniae in the respective specimens and the studied inflammatory markers or the presence of anti‐M pneumoniae antibodies was not observed. H pylori‐specific DNA could not be detected in the specimens tested.
Conclusions
The absence of H pylori and the random distribution of M pneumoniae in tissue samples obtained from patients with symptomatic carotid artery stenosis do not support a role for these pathogens in the development of atherosclerosis due to a direct interaction of the bacteria with the vasculature.
doi:10.1136/jcp.2005.034314
PMCID: PMC1860507  PMID: 16644879
2.  Specific immunoprophylaxis in experimental tumour-host systems. 
A variety of animal species have been rendered resistant to syngeneic tumours of many histologic types of immunoprophylaxis. Among the types of preparation of tumour-associated antigens that have merit as vaccines are tumour cells treated with radiation, mitomycin C, certain viruses, neuraminidase, sulfhydryl blocking agents and lipoidal reagents. Alternatively, tumour-associated antigens of the cell membrane may be solubilized and used for vaccination. Recent studies with dimethyldioctadecylammonium bromide (DDA) indicate that it both modifies tumour cells and serves as an immunologic adjuvant in that it enhances protective responses to iodoacetamide-treated lymphoma cells and acts as a potent macrophage activator. By judicious application of DDA either delayed hypersensitivity or antibody response may be selectively enhanced. Several advantages of DDA over other adjuvants are its water solubility, the fact that it does not produce deleterious lesions at the site of injection and the fact that it eliminates the risk of systemic infection that exists with the use of live bacteria.
PMCID: PMC1801890  PMID: 7363246

Results 1-2 (2)