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Journal of Neurogenetics (1)
Masuyama, Kaoru (2)
Enell, Lina E. (1)
Green, David S. (1)
Lee, Chi-Hon (1)
Nässel, Dick R. (1)
Rao, Yi (1)
Root, Cory M. (1)
Wang, Jing W (1)
Wang, Jing W. (1)
Zhang, Yi (1)
Year of Publication
A Presynaptic Gain Control Mechanism Fine-Tunes Olfactory Behavior
Root, Cory M.
Green, David S.
Enell, Lina E.
Nässel, Dick R.
Wang, Jing W.
Early sensory processing can play a critical role in sensing environmental cues. We have investigated the physiological and behavioral function of gain control at the first synapse of olfactory processing in Drosophila. We report that olfactory receptor neurons (ORNs) express the GABAB receptor (GABABR) and its expression expands the dynamic range of ORN synaptic transmission that is preserved in projection neuron responses. Strikingly, we find that different ORN channels have unique baseline levels of GABABR expression. ORNs that sense the aversive odorant CO2 do not express GABABRs nor exhibit any presynaptic inhibition. In contrast, pheromone-sensing ORNs express a high level of GABABRs and exhibit strong presynaptic inhibition. Furthermore, a behavioral significance of presynaptic inhibition was revealed by a courtship behavior in which pheromone-dependent mate localization is impaired in flies that lack GABABRs in specific ORNs. Together, these findings indicate that different olfactory receptor channels may employ heterogeneous presynaptic gain control as a mechanism to allow an animal’s innate behavioral responses to match its ecological needs.
Drosophila; olfaction; GABAB; presynaptic inhibition; gain control; dynamic range; two-photon imaging
Mapping Neural Circuits with Activity-Dependent Nuclear Import of a Transcription Factor
Wang, Jing W
Journal of Neurogenetics
Nuclear factor of activated T cells (NFAT) is a calcium-responsive transcription factor. We describe here an NFAT-based neural tracing method—CaLexA (calcium-dependent nuclear import of Lex A)—for labeling active neurons in behaving animals. In this system, sustained neural activity induces nuclear import of the chimeric transcription factor LexA-VP16-NFAT, which in turn drives green fluorescent protein (GFP) reporter expression only in active neurons. We tested this system in Drosophila and found that volatile sex pheromones excite specific neurons in the olfactory circuit. Furthermore, complex courtship behavior associated with multi-modal sensory inputs activated neurons in the ventral nerve cord. This method harnessing the mechanism of activity-dependent nuclear import of a transcription factor can be used to identify active neurons in specific neuronal population in behaving animals.
Drosophila; olfaction; antennal lobe; pheromone; NFAT; activity dependent; N2A; immediate-early gene
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