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AMIA Annual Symposium Proceedings (1)
Asian Journal of Andrology (1)
Case Reports in Obstetrics and Gynecology (1)
The Open Medical Informatics Journal (1)
Lunenfeld, Eitan (4)
Abu Elhija, Mahmoud (1)
Baron, Joel (1)
Bu, Davis (1)
Cantor, Michael (1)
Har-Vardi, Iris (1)
Hatsek, Avner (1)
Hripcsak, George (1)
Huleihel, Mahmoud (1)
Kaiser, Gail E. (1)
Klimov, Denis (1)
Landesberg, Giora (1)
Levitas, Eliahu (1)
Man, Limor (1)
Patel, Vimla L. (1)
Peleg, Mor (1)
Schlatt, Stefan (1)
Shahar, Yuval (1)
Shalom, Erez (1)
Shortliffe, Edward H. (1)
Taieb-Maimon, Meirav (1)
Tu, Samson W. (1)
Wang, Dongwen (1)
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A Live Birth Subsequent to IVF following Egg Retrieval Only 12 Hours after hCG Priming
Case Reports in Obstetrics and Gynecology
Introduction. To report a live birth following egg retrieval after only 12 hours from hCG priming. Patients. A childless couple with five-years-lasting secondary infertility. Methods. IVF was performed according to the long protocol. Two immature oocytes were retrieved following only 12 hours after hCG priming due to the patient misunderstanding. The eggs were cultured in vitro and ICSI was performed following polar body extruded after 24 hours in culture. After additional 24 hours a 4-cell embryo was developed and ET was performed. Results. A viable pregnancy was achieved and a healthy baby girl was delivered at 38 weeks of gestation. Conclusion. In a rare and unexpected situation when immature oocytes are retrieved following a short hCG priming, the eggs should be cultured in vitro, late ICSI should be performed, and a pregnancy may be expected.
Differentiation of murine male germ cells to spermatozoa in a soft agar culture system
Abu Elhija, Mahmoud
Asian Journal of Andrology
Establishment of an in vitro system that allows the development of testicular germ cells to sperm will be valuable for studies of spermatogenesis and future treatments for male infertility. In the present study, we developed in vitro culture conditions using three-dimensional agar culture system (SACS), which has the capacity to induce testicular germ cells to reach the final stages of spermatogenesis, including spermatozoa generation. Seminiferous tubules from testes of 7-day-old mice were enzymatically dissociated, and intratubular cells were cultured in the upper layer of the SACS in RPMI medium supplemented with fetal calf serum (FCS). The lower layer of the SACS contained only RPMI medium supplemented with FCS. Colonies in the upper layer were isolated after 14 and 28 days of culture and were classified according to their size. Immunofluorescence and real-time PCR were used to analyse specific markers expressed in undifferentiated and differentiated spermatogonia (Vasa, Dazl, OCT-4, C-Kit, GFR-α-1, CD9 and α-6-integrin), meiotic cells (LDH, Crem-1 and Boule) and post-meiotic cells (Protamine-1, Acrosin and SP-10). Our results reveal that it is possible to induce mouse testicular pre-meiotic germ cell expansion and induce their differentiation to spermatozoa in SACS. The spermatozoa showed normal morphology and contained acrosomes. Thus, our results demonstrate that SACS could be used as a novel in vitro system for the maturation of pre-meiotic mouse germ cells to post-meiotic stages and morphologically-normal spermatozoa.
acrosome; agar; in vitro culture; meiosis; spermatogenesis; spermatogonia; spermatozoa; testis
A Scalable Architecture for Incremental Specification and Maintenance of Procedural and Declarative Clinical Decision-Support Knowledge
The Open Medical Informatics Journal
Clinical guidelines have been shown to improve the quality of medical care and to reduce its costs. However, most guidelines exist in a free-text representation and, without automation, are not sufficiently accessible to clinicians at the point of care. A prerequisite for automated guideline application is a machine-comprehensible representation of the guidelines. In this study, we designed and implemented a scalable architecture to support medical experts and knowledge engineers in specifying and maintaining the procedural and declarative aspects of clinical guideline knowledge, resulting in a machine comprehensible representation. The new framework significantly extends our previous work on the Digital electronic Guidelines Library (DeGeL) The current study designed and implemented a graphical framework for specification of declarative and procedural clinical knowledge, Gesher. We performed three different experiments to evaluate the functionality and usability of the major aspects of the new framework: Specification of procedural clinical knowledge, specification of declarative clinical knowledge, and exploration of a given clinical guideline. The subjects included clinicians and knowledge engineers (overall, 27 participants). The evaluations indicated high levels of completeness and correctness of the guideline specification process by both the clinicians and the knowledge engineers, although the best results, in the case of declarative-knowledge specification, were achieved by teams including a clinician and a knowledge engineer. The usability scores were high as well, although the clinicians’ assessment was significantly lower than the assessment of the knowledge engineers.
Medical informatics; clinical guidelines; decision support systems; knowledge representation; knowledge acquisition; knowledge bases; ontologies; information retrieval; human computer interaction; artificial intelligence; digital libraries; service oriented architecture.
GESDOR – A Generic Execution Model for Sharing of Computer-Interpretable Clinical Practice Guidelines
Tu, Samson W.
Kaiser, Gail E.
Patel, Vimla L.
Shortliffe, Edward H.
AMIA Annual Symposium Proceedings
We developed the Guideline Execution by Semantic Decomposition of Representation (GESDOR) model to share guidelines encoded in different formats at the execution level. For this purpose, we extracted a set of generalized guideline execution tasks from the existing guideline representation models. We then created the mappings between specific guideline representation models and the set of the common guideline execution tasks. Finally, we developed a generic task-scheduling model to harmonize the existing approaches to guideline task scheduling. The evaluation has shown that the GESDOR model can be used for the effective execution of guidelines encoded in different formats, and thus realizes guideline sharing at the execution level.
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