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1.  Neutralization of Lethality and Proteolytic Activities of Malayan Pit Viper (Calloselasma rhodostoma) Venom with North American Virginia Opossum (Didelphis virginiana) Serum 
Malayan pit viper (Calloselasma rhodostoma) envenomation is a major health problem in South East Asia. During envenomation, venom components mainly affect the hemostatic system. The sera from the North American Virginia opossums (Didelphis virginiana) were able to neutralize the venom of the Malayan pit viper. These natural inhibitors could be explored as potential therapeutics against envenomations of a variety of venomous snake species in different geographical habitats.
PMCID: PMC3437917  PMID: 18617212
snake; venom; Malayan pit viper; Calloselasma rhodostoma; Virginia opossum; Didelphis virginiana; antifibrinolytic; antihemorrhagic; antigelatinase; LD50; ED50
2.  Purification of a phospholipase A2 from Daboia russelii siamensis venom with anticancer effects 
Venom phospholipases A2 (PLA2) are associated with neurotoxic, myotoxic, cardiotoxic, platelet aggregation, and edema activities. A PLA2 (Drs-PLA2) was purified from Daboia russelii siamensis venom by a two-step purification procedure consisting of size-exclusion, followed by anion exchange high performance liquid chromatography (HPLC). The molecular weight of the Drs-PLA2 was 13,679Da, which was determined by MALDI-TOF mass spectrometry. Its N-terminal amino acid sequence was homologous to basic PLA2s of viperid snake venoms. The Drs-PLA2 had indirect hemolytic and anticoagulant activities, cytotoxic activity with a CC50 of 65.8nM, and inhibited SK-MEL-28 cell migration with an IC50 of 25.6nM. In addition, the Drs-PLA2 inhibited the colonization of B16F10 cells in lungs of BALB/c mice by ∼65%.
PMCID: PMC3210966  PMID: 22091349
Daboia russelii siamensis; phospholipase A2; cytotoxic; cell migration inhibition; lung tumor colonization
3.  Bat Nipah Virus, Thailand 
Emerging Infectious Diseases  2005;11(12):1949-1951.
Surveillance for Nipah virus (NV) was conducted in Thailand's bat population. Immunoglobulin G antibodies to NV were detected with enzyme immunoassay in 82 of 1,304 bats. NV RNA was found in bat saliva and urine. These data suggest the persistence of NV infection in Thai bats.
PMCID: PMC3367639  PMID: 16485487
Nipah virus; Hendra virus; RNA virus; bat; chiroptera; zoonosis; animals; Thailand; serology; dispatch
4.  Survey for Bat Lyssaviruses, Thailand 
Emerging Infectious Diseases  2005;11(2):232-236.
Surveillance for lyssaviruses was conducted among bat populations in 8 provinces in Thailand. In 2002 and 2003, a total of 932 bats of 11 species were captured and released after serum collection. Lyssavirus infection was determined by conducting virus neutralization assays on bat serum samples. Of collected samples, 538 were either hemolysed or insufficient in volume, which left 394 suitable for analysis. These samples included the following: Pteropus lylei (n = 335), Eonycteris spelaea (n = 45), Hipposideros armiger (n = 13), and Rousettus leschennaulti (n = 1). No serum samples had evidence of neutralizing antibodies when tested against rabies virus. However, 16 samples had detectable neutralizing antibodies against Aravan virus, Khujand virus, Irkut virus, or Australian bat lyssavirus; all were specifically associated with fruit bats P. lylei (n = 15) and E. spelaea (n = 1). These results are consistent with the presence of naturally occurring viruses related to new putative lyssavirus genotypes.
PMCID: PMC3320458  PMID: 15752440
Lyssavirus; rabies; RNA; bat; chiroptera; zoonosis; animals; fluorescent antibody technique; direct/veterinary; Thailand; research

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