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author:("avellino, Ana")
1.  Paralysis Case and Contact Spread of Recombinant Vaccine–derived Poliovirus, Spain 
Emerging Infectious Diseases  2008;14(11):1807-1809.
doi:10.3201/eid1411.080517
PMCID: PMC2630745  PMID: 18976579
poliovirus; poliomyelitis; enterovirus; VDPV; letter
2.  Endemic Circulation of European Bat Lyssavirus Type 1 in Serotine Bats, Spain 
Emerging Infectious Diseases  2008;14(8):1263-1266.
To determine the presence of European bat lyssavirus type 1 in southern Spain, we studied 19 colonies of serotine bats (Eptesicus isabellinus), its main reservoir, during 1998–2003. Viral genome and antibodies were detected in healthy bats, which suggests subclinical infection. The different temporal patterns of circulation found in each colony indicate independent endemic circulation.
doi:10.3201/1408.080068
PMCID: PMC2600403  PMID: 18680651
lyssavirus; bats; surveillance; rabies; dispatch
3.  Enterovirus 75 and Aseptic Meningitis, Spain, 2005 
Emerging Infectious Diseases  2006;12(10):1609-1611.
doi:10.3201/eid1210.060353
PMCID: PMC3290945  PMID: 17176588
Enterovirus; Enterovirus type 75; EV75; Aseptic meningitis; letter
4.  Molecular Analysis of Echovirus 13 Isolates and Aseptic Meningitis, Spain 
Emerging Infectious Diseases  2003;9(8):934-941.
Echovirus 13 (EV13), considered rare, was reported worldwide in 2000, mostly related to aseptic meningitis outbreaks. In Spain, 135 EV13 isolates were identified. The genetic relationships between 64 representative strains from Spain and other reported isolates from the United States, Germany, Italy, Japan, and Sweden were described by analyzing the partial sequence of the major capsid protein (VP1) gene. The strains from Spain were clearly identified as EV13 (79.5% similarity with the EV13 reference strain) and were grouped phylogenetically into two different clusters (by origination on either the Iberian Peninsula or Canary Islands). Isolates from Germany from 2000 clustered with the Canary Islands group. The isolates from other countries obtained before 2000 were genetically distant. Changes in EV13 coding sequence involved several differences in the C-terminal extreme of the VP1 protein. Part of the neutralizing antigenic site III has been described in this genome region in poliovirus and swine vesicular disease virus.
doi:10.3201/eid0908.030080
PMCID: PMC3020609  PMID: 12967490
Enterovirus, human; echovirus, Echovirus 13, Meningitis, Viral; Meningitis, Aseptic, Molecular Epidemiology, Spain, Canary Islands , research
5.  Screening of Active Lyssavirus Infection in Wild Bat Populations by Viral RNA Detection on Oropharyngeal Swabs 
Journal of Clinical Microbiology  2001;39(10):3678-3683.
Brain analysis cannot be used for the investigation of active lyssavirus infection in healthy bats because most bat species are protected by conservation directives. Consequently, serology remains the only tool for performing virological studies on natural bat populations; however, the presence of antibodies merely reflects past exposure to the virus and is not a valid marker of active infection. This work describes a new nested reverse transcription (RT)-PCR technique specifically designed for the detection of the European bat virus 1 on oropharyngeal swabs obtained from bats but also able to amplify RNA from the remaining rabies-related lyssaviruses in brain samples. The technique was successfully used for surveillance of a serotine bat (Eptesicus serotinus) colony involved in a case of human exposure, in which 15 out of 71 oropharyngeal swabs were positive. Lyssavirus infection was detected on 13 oropharyngeal swabs but in only 5 brains out of the 34 animals from which simultaneous brain and oropharyngeal samples had been taken. The lyssavirus involved could be rapidly identified by automatic sequencing of the RT-PCR products obtained from 14 brains and three bat oropharyngeal swabs. In conclusion, RT-PCR using oropharyngeal swabs will permit screening of wild bat populations for active lyssavirus infection, for research or epidemiological purposes, in line not only with conservation policies but also in a more efficient manner than classical detection techniques used on the brain.
doi:10.1128/JCM.39.10.3678-3683.2001
PMCID: PMC88406  PMID: 11574590

Results 1-5 (5)