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1.  Brassicaceous Seed Meals as Soil Amendments to Suppress the Plant-parasitic Nematodes Pratylenchus penetrans and Meloidogyne incognita1 
Journal of Nematology  2009;41(3):221-227.
Brassicaceous seed meals are the residual materials remaining after the extraction of oil from seeds; these seed meals contain glucosinolates that potentially degrade to nematotoxic compounds upon incorporation into soil. This study compared the nematode-suppressive ability of four seed meals obtained from Brassica juncea ‘Pacific Gold’, B. napus ‘Dwarf Essex’ and ‘Sunrise’, and Sinapis alba ‘IdaGold’, against mixed stages of Pratylenchus penetrans and Meloidogyne incognita second-stage juveniles (J2). The brassicaceous seed meals were applied to soil in laboratory assays at rates ranging from 0.5 to 10.0% dry w/w with a nonamended control included. Nematode mortality was assessed after 3 days of exposure and calculated as percentage reduction compared to a nonamended control. Across seed meals, M. incognita J2 were more sensitive to the brassicaceous seed meals compared to mixed stages of P. penetrans. Brassica juncea was the most nematode-suppressive seed meal with rates as low as 0.06% resulting in > 90% suppression of both plant-parasitic nematodes. In general B. napus ‘Sunrise’ was the least nematode-suppressive seed meal. Intermediate were the seed meals of S. alba and B. napus ‘Dwarf Essex’; 90% suppression was achieved at 1.0% and 5.0% S. alba and 0.25% and 2.5% B. napus ‘Dwarf Essex’, for M. incognita and P. penetrans, respectively. For B. juncea, seed meal glucosinolate-degradation products appeared to be responsible for nematode suppression; deactivated seed meal (wetted and heated at 70 °C for 48 hr) did not result in similar P. penetrans suppression compared to active seed meal. Sinapis alba seed meal particle size also played a role in nematode suppression with ground meal resulting in 93% suppression of P. penetrans compared with 37 to 46% suppression by pelletized S. alba seed meal. This study demonstrates that all seed meals are not equally suppressive to nematodes and that care should be taken when selecting a source of brassicaceous seed meal for plant-parasitic nematode management.
PMCID: PMC3380494  PMID: 22736818
amendment; isothiocyanate; glucosinolate; Pratylenchus penetrans; Meloidogyne incognita; brassica; seed meal
2.  Evaluation of Cover Crops with Potential for Use in Anaerobic Soil Disinfestation (ASD) for Susceptibility to Three Species of Meloidogyne 
Journal of Nematology  2013;45(4):272-278.
Several cover crops with potential for use in tropical and subtropical regions were assessed for susceptibility to three common species of root-knot nematode, Meloidogyne arenaria, M. incognita, and M. javanica. Crops were selected based on potential use as organic amendments in anaerobic soil disinfestation (ASD) applications. Nematode juvenile (J2) numbers in soil and roots, egg production, and host plant root galling were evaluated on arugula (Eruca sativa, cv. Nemat), cowpea (Vigna unguiculata, cv. Iron & Clay), jack bean (Canavalia ensiformis, cv. Comum), two commercial mixtures of Indian mustard and white mustard (Brassica juncea & Sinapis alba, mixtures Caliente 61 and Caliente 99), pearl millet (Pennisetum glaucum, cv. Tifleaf III), sorghum-sudangrass hybrid (Sorghum bicolor × S. bicolor var. sudanense, cv. Sugar Grazer II), and three cultivars of sunflower (Helianthus annuus, cvs. 545A, Nusun 660CL, and Nusun 5672). Tomato (Solanum lycopersicum, cv. Rutgers) was included in all trials as a susceptible host to all three nematode species. The majority of cover crops tested were less susceptible than tomato to M. arenaria, with the exception of jack bean. Sunflower cv. Nusun 5672 had fewer M. arenaria J2 isolated from roots than the other sunflower cultivars, less galling than tomato, and fewer eggs than tomato and sunflower cv. 545A. Several cover crops did not support high populations of M. incognita in roots or exhibit significant galling, although high numbers of M. incognita J2 were isolated from the soil. Arugula, cowpea, and mustard mixture Caliente 99 did not support M. incognita in soil or roots. Jack bean and all three cultivars of sunflower were highly susceptible to M. javanica, and all sunflower cultivars had high numbers of eggs isolated from roots. Sunflower, jack bean, and both mustard mixtures exhibited significant galling in response to M. javanica. Arugula, cowpea, and sorghum-sudangrass consistently had low numbers of all three Meloidogyne species associated with roots and are good selections for use in ASD for root-knot nematode control. The remainder of crops tested had significant levels of galling, J2, and eggs associated with roots, which varied among the Meloidogyne species tested.
PMCID: PMC3873904  PMID: 24379486
Anaerobic soil disinfestation; ASD; Brassica juncea & Sinapis alba; Canavalia ensiformis; cover crops; cowpea; Eruca sativa; Helianthus annuus; jack bean; management; Meloidogyne arenaria; M. incognita; M. javanica; mustard; pearl millet; Pennisetum glaucum; root-knot nematodes; sorghum-sudangrass; sunflower; Vigna unguiculata
3.  Activity of Meadowfoam (Limnanthes alba) Seed Meal Glucolimnanthin Degradation Products against Soil-Borne Pathogens 
Meadowfoam (Limnanthes alba L.) is a herbaceous winter-spring annual grown as a commercial oil seed crop. The meal remaining after oil extraction from the seed contains up to 4% of the glucosinolate, glucolimnanthin. Degradation of glucolimnanthin yields toxic breakdown products and therefore the meal may have potential in the management of soil-borne pathogens. To maximize the pest suppressive potential of meadowfoam seed meal, it would be beneficial to know the toxicity of individual glucolimnanthin degradation products against specific soil-borne pathogens. Meloidogyne hapla second-stage juveniles (J2) and Pythium irregulare and Verticillium dahliae mycelial cultures were exposed to glucolimnanthin as well as its degradation products. Glucolimnanthin and its degradation product, 2-(3-methoxyphenyl)acetamide, were not toxic to any of the soil-borne pathogens at concentrations up to 1.0 mg/mL. Two other degradation products, 2-(3-methoxymethyl)ethanethioamide and 3-methoxyphenylacetonitrile, were toxic to M. hapla and P. irregulare but not V. dahliae. The predominant enzyme degradation product, 3-methoxybenzyl isothiocyanate was the most toxic compound against all of the soil-borne pathogens with M. hapla being the most sensitive with EC50 values (0.0025 ± 0.0001 to 0.0027 ± 0.0001 mg/mL) 20 to 40 times lower than estimated EC50 mortality values generated for P. irregulare and V. dahliae (0.05 and 0.1 mg/mL, respectively). The potential exists to manipulate meadowfoam seed meal to promote the production of specific degradation products. The conversion of glucolimnanthin into its corresponding isothiocyanate should optimize the biopesticidal properties of meadowfoam seed meal against M. hapla, P. irregulare, and V. dahliae.
PMCID: PMC4215540  PMID: 22142246
nematicidal effect; fungicidal effect; lethal concentration; seed meal
4.  Effect of Crotalaria juncea Amendment on Squash Infected with Meloidogyne incognita 
Journal of Nematology  2004;36(3):290-296.
Two greenhouse experiments were conducted to examine the effect of Crotalaria juncea amendment on Meloidogyne incognita population levels and growth of yellow squash (Cucurbita pepo). In the first experiment, four soils with a long history of receiving yard waste compost (YWC+), no-yard-waste compost (YWC-), conventional tillage, or no-tillage treatments were used; in the second experiment, only one recently cultivated soil was used. Half of the amount of each soil received air-dried residues of C. juncea as amendment before planting squash, whereas the other half did not. Crotalaria juncea amendment increased squash shoot and root weights in all soils tested, except in YWC+ soil where the organic matter content was high without the amendment. The amendment suppressed the numbers of M. incognita if the inoculum level was low, and when the soil contained relatively abundant nematode-antagonistic fungi. Microwaved soil resulted in greater numbers of M. incognita and free-living nematodes than frozen or untreated soil, indicating nematode-antagonistic microorganisms played a role in nematode suppression. The effects of C. juncea amendment on nutrient cycling were complex. Amendment with C. juncea increased the abundance of free-living nematodes and Harposporium anguillulae, a fungus antagonistic to them in the second experiment but not in the first experiment. Soil histories, especially long-term yard waste compost treatments that increased soil organic matter, can affect the performance of C. juncea amendment.
PMCID: PMC2620769  PMID: 19262819
free-living nematode; nematode-trapping fungi; organic amendments; root-knot nematode; soil ecosystem; soil nutrient; sunn hemp; tillage
5.  Effects of Black Pepper (Piper Nigrum), Turmeric Powder (Curcuma Longa) and Coriander Seeds (Coriandrum Sativum) and Their Combinations as Feed Additives on Growth Performance, Carcass Traits, Some Blood Parameters and Humoral Immune Response of Broiler Chickens 
Different herbs and spices have been used as feed additives for various purposes in poultry production. This study was conducted to assess the effect of feed supplemented with black pepper (Piper nigrum), turmeric powder (Curcuma longa), coriander seeds (Coriandrum sativum) and their combinations on the performance of broilers. A total of 210 (Cobb) one-d-old chicks were divided into seven groups of 30 birds each. The treatments were: a control group received no supplement, 0.5% black pepper (T1), 0.5% turmeric powder (T2), 2% coriander seeds (T3), a mixture of 0.5% black pepper and 0.5% turmeric powder (T4), a mixture of 0.5% black pepper and 2% coriander seed (T5), and a mixture of 0.5% black pepper, 0.5% turmeric powder and 2% coriander seeds (T6). Higher significant values of body weight gain during the whole period of 5 weeks (p<0.001) were observed in broilers on T1, T3, T5, and T6 compared to control. Dietary supplements with T1, T2, T3, and T6 improved the cumulative G:F of broilers during the whole period of 5 weeks (p<0.001) compared with control. The dressing percentage and edible giblets were not influenced by dietary supplements, while higher values of relative weight of the liver (p<0.05) were obtained in T5 and T6 compared to control. The addition of feed supplements in T5 and T6 significantly increased serum total protein and decreased serum glucose, triglycerides and alkaline phosphatase concentrations compared with the control group (p<0.05). Broilers on T6 showed significant decrease in the serum glutamate pyruvate transaminase concentration (p<0.05) compared to control. The broilers having T5 and T6 supplemented feed had relatively greater antibody titre (p<0.001) at 35 d of age than control. It is concluded that dietary supplements with black pepper or coriander seeds or their combinations enhanced the performance and health status of broiler chickens.
PMCID: PMC4093167  PMID: 25050023
Antibody Titre; Blood Biochemistry; Carcass; Chicken; Herbs; Performance
6.  Host Plant Resistance as an Alternative to Methyl Bromide for Managing Meloidogyne incognita in Pepper 
Journal of Nematology  2002;34(4):374-377.
Pre-plant soil fumigation with methyl bromide and host resistance were compared for managing the southern root-knot nematode (Meloidogyne incognita) in pepper. Three pepper cultivars (Carolina Cayenne, Keystone Resistant Giant, and California Wonder) that differed in resistance to M. incognita were grown in field plots that had been fumigated with methyl bromide (98% CH₃Br : 2% CCl₃NO₂ [w/w]) before planting or left untreated. Carolina Cayenne is a well-adapted cayenne-type pepper that is highly resistant to M. incognita. The bell-type peppers Keystone Resistant Giant and California Wonder are intermediate to susceptible and susceptible, respectively. None of the cultivars exhibited root galling in the methyl bromide fumigated plots and nematode reproduction was minimal (<250 eggs/g fresh root), indicating that the fumigation treatment was highly effective in controlling M. incognita. Root galling of Carolina Cayenne and nematode reproduction were minimal, and fruit yields were not reduced in the untreated plots. The root-galling reaction for Keystone Resistant Giant was intermediate (gall index = 2.9, on a scale of 1 to 5), and nematode reproduction was moderately high. However, yields of Keystone Resistant Giant were not reduced in untreated plots. Root galling was severe (gall index = 4.3) on susceptible California Wonder, nematode reproduction was high, and fruit yields were reduced (P ≤ 0.05) in untreated plots. The resistance exhibited by Carolina Cayenne and Keystone Resistant Giant provides an alternative to methyl bromide for reducing yield losses by southern root-knot nematodes in pepper. The high level of resistance of Carolina Cayenne also suppresses population densities of M. incognita.
PMCID: PMC2620592  PMID: 19265959
Capsicum annuum; Meloidogyne incognita; methyl bromide alternatives; nematode management; nematode resistance; pepper; root-knot nematodes
7.  Development of Multi-Component Transplant Mixes for Suppression of Meloidogyne incognita on Tomato (Lycopersicon esculentum) 
Journal of Nematology  2002;34(4):362-369.
The effects of combinations of organic amendments, phytochemicals, and plant-growth promoting rhizobacteria on tomato (Lycopersicon esculentum) germination, transplant growth, and infectivity of Meloidogyne incognita were evaluated. Two phytochemicals (citral and benzaldehyde), three organic amendments (pine bark, chitin, and hemicellulose), and three bacteria (Serratia marcescens, Brevibacterium iodinum, and Pseudomonas fluorescens) were assessed. Increasing rates of benzaldehyde and citral reduced nematode egg viability in vitro. Benzaldehyde was 100% efficacious as a nematicide against juveniles, whereas citral reduced juvenile viability to less than 20% at all rates tested. Benzaldehyde increased tomato seed germination and root weight, whereas citral decreased both. High rates of pine bark or chitin reduced plant growth but not seed germination, whereas low rates of chitin increased shoot length, shoot weight, and root weight; improved root condition; and reduced galling. The combination of chitin and benzaldehyde significantly improved tomato transplant growth and reduced galling. While each of the bacterial isolates contributed to increased plant growth in combination treatments, only Brevibacterium iodinum applied alone significantly improved plant growth.
PMCID: PMC2620595  PMID: 19265957
benzaldehyde; Brevibacterium iodinum; chitin; citral; hemicellulose; Lycopersicon esculentum; phytochemicals; pine bark; Pseudomonas fluorescens; rhizobacteria; root-knot nematode; Serratia marcescens; tomato; transplants
8.  Effect of Meloidogyne incognita and Importance of the Inoculum on the Yield of Eggplant 
Journal of Nematology  1986;18(4):487-490.
The relationship between population densities of race 1 of Meloidogyne incognita and yield of eggplant was studied. Microplots were infested with finely chopped nematode-infected pepper roots to give population densities of 0, 0.062, 0.125, 0.25, 0.50, 1, 2, 4, 8, 16, 32, 64, and 128 eggs and juveniles/cm³ soil. Both plant growth and yield were suppressed by the nematode. A tolerance limit of 0.054 eggs and juveniles/cm³ soil and a minimum relative yield of 0.05 at four or more eggs and juveniles/cm³ soil were derived by fitting the data with the equation y = m + (1 - m)zP⁻T. Maximum nematode reproduction rate was 12,300. Hatch of eggs from egg masses in water or from sodium hypochlorite dissolved egg masses was similar (41% and 39%), but egg viability was significantly greater from egg masses in water (58%) than from sodium hypochlorite dissolved egg masses (12%) after 4 weeks. Greater numbers of nematodes were collected from roots of tomatoes from soil infested with entire egg masses than from tomato roots from soil infested with egg masses dissolved by sodium hypochlorite.
PMCID: PMC2618571  PMID: 19294216
root-knot nematode; tolerance limit; eggplant; inoculum; Solanum melongena; Meloidogyne incognita
9.  Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions 
Annals of Botany  2012;110(6):1149-1159.
Background and Aims
Despite differences in physiology between dry and relative moist seeds, seed ageing tests most often use a temperature and seed moisture level that are higher than during dry storage used in commercial practice and gene banks. This study aimed to test whether seed ageing under dry conditions can be accelerated by storing under high-pressure oxygen.
Dry barley (Hordeum vulgare), cabbage (Brassica oleracea), lettuce (Lactuca sativa) and soybean (Glycine max) seeds were stored between 2 and 7 weeks in steel tanks under 18 MPa partial pressure of oxygen. Storage under high-pressure nitrogen gas or under ambient air pressure served as controls. The method was compared with storage at 45 °C after equilibration at 85 % relative humidity and long-term storage at the laboratory bench. Germination behaviour, seedling morphology and tocopherol levels were assessed.
Key Results
The ageing of the dry seeds was indeed accelerated by storing under high-pressure oxygen. The morphological ageing symptoms of the stored seeds resembled those observed after ageing under long-term dry storage conditions. Barley appeared more tolerant of this storage treatment compared with lettuce and soybean. Less-mature harvested cabbage seeds were more sensitive, as was the case for primed compared with non-primed lettuce seeds. Under high-pressure oxygen storage the tocopherol levels of dry seeds decreased, in a linear way with the decline in seed germination, but remained unchanged in seeds deteriorated during storage at 45 °C after equilibration at 85 % RH.
Seed storage under high-pressure oxygen offers a novel and relatively fast method to study the physiology and biochemistry of seed ageing at different seed moisture levels and temperatures, including those that are representative of the dry storage conditions as used in gene banks and commercial practice.
PMCID: PMC3478056  PMID: 22967856
Oxidative stress; seed ageing; seed longevity; seed quality; seed storage; seed test; tocopherol
10.  Effect of Crotalaria juncea Amendment on Nematode Communities in Soil with Different Agricultural Histories 
Journal of Nematology  2003;35(3):294-301.
Effect of sunn hemp (Crotalaria juncea) hay amendment on nematode community structure in the soil surrounding roots of yellow squash (Cucurbita pepo) infected with root-knot nematodes was examined in two greenhouse experiments. Soils were from field plots treated long-term (LT) with yard-waste compost or no yard-waste compost in LT experiment, and from a short-term (ST) agricultural site in ST experiment. Soils collected were either amended or not amended with C. juncea hay. Nematode communities were examined 2 months after squash was inoculated with Meloidogyne incognita. Amendment increased (P < 0.05) omnivorous nematodes in both experiments but increased only bacterivorous nematodes in ST experiment (P < 0.05), where the soil had relatively low organic matter (<2%). This effect of C. juncea amendment did not occur in LT experiment, in which bacterivores were already abundant. Fungivorous nematodes were not increased by C. juncea amendment in either experiment, but predatory nematodes were increased when present. Although most nematode faunal indices, including enrichment index, structure index, and channel index, were not affected by C. juncea amendment, structure index values were affected by previous soil organic matter content. Results illustrate the importance of considering soil history (organic matter, nutrient level, free-living nematode number) in anticipating changes following amendment with C. juncea hay.
PMCID: PMC2620648  PMID: 19262764
community structure indices; Cucurbita pepo; Meloidogyne incognita; organic amendments; squash; sunn hemp
11.  Manipulation of Rhizosphere Bacterial Communities to Induce Suppressive Soils 
Journal of nematology  2007;39(3):213-220.
Naturally occurring disease-suppressive soils have been documented in a variety of cropping systems, and in many instances the biological attributes contributing to suppressiveness have been identified. While these studies have often yielded an understanding of operative mechanisms leading to the suppressive state, significant difficulty has been realized in the transfer of this knowledge into achieving effective field-level disease control. Early efforts focused on the inundative application of individual or mixtures of microbial strains recovered from these systems and known to function in specific soil suppressiveness. However, the introduction of biological agents into non-native soil ecosystems typically yielded inconsistent levels of disease control. Of late, greater emphasis has been placed on manipulation of the cropping system to manage resident beneficial rhizosphere microorganisms as a means to suppress soilborne plant pathogens. One such strategy is the cropping of specific plant species or genotypes or the application of soil amendments with the goal of selectively enhancing disease-suppressive rhizobacteria communities. This approach has been utilized in a system attempting to employ biological elements resident to orchard ecosystems as a means to control the biologically complex phenomenon termed apple replant disease. Cropping of wheat in apple orchard soils prior to re-planting the site to apple provided control of the fungal pathogen Rhizoctonia solani AG-5. Disease control was elicited in a wheat cultivar-specific manner and functioned through transformation of the fluorescent pseudomonad population colonizing the rhizosphere of apple. Wheat cultivars that induced disease suppression enhanced populations of specific fluorescent pseudomonad genotypes with antagonistic activity toward R. solani AG-5, but cultivars that did not elicit a disease-suppressive soil did not modify the antagonistic capacity of this bacterial community. Alternatively, brassicaceae seed meal amendments were utilized to develop soil suppressiveness toward R. solani. Suppression of Rhizoctonia root rot in response to seed meal amendment required the activity of the resident soil microbiota and was associated with elevated populations of Streptomyces spp. recovered from the apple rhizosphere. Application of individual Streptomyces spp. to soil systems provided control of R. solani to a level and in a manner equivalent to that obtained with the seed meal amendment. These and other examples suggest that management of resident plant-beneficial rhizobacteria may be a viable method for control of specific soilborne plant pathogens.
PMCID: PMC2586500  PMID: 19259490
suppressive soils; biological control; replant disease; rhizobacteria
12.  Characterization of streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. 
The actinomycete Streptomyces lydicus WYEC108 showed strong in vitro antagonism against various fungal plant pathogens in plate assays by producing extracellular antifungal metabolites. When Pythium ultimum or Rhizoctonia solani was grown in liquid medium with S. lydicus WYEC108, inhibition of growth of the fungi was observed. When WYEC108 spores or mycelia were used to coat pea seeds, the seeds were protected from invasion by P. ultimum in an oospore-enriched soil. While 100% of uncoated control seeds were infected by P. ultimum within 48 h after planting, less than 40% of coated seeds were infected. When the coated seeds were planted in soil 24 h prior to introduction of the pathogen, 96 h later, less than 30% of the germinating seeds were infected. Plant growth chamber studies were also carried out to test for plant growth effects and for suppression by S. lydicus WYEC108 of Pythium seed rot and root rot. When WYEC108 was applied as a spore-peat moss-sand formulation (10(8) CFU/g) to P. ultimum-infested sterile or nonsterile soil planted with pea and cotton seeds, significant increases in average plant stand, plant length, and plant weight were observed in both cases compared with untreated control plants grown in similar soils. WYEC108 hyphae colonized and were able to migrate downward with the root as it elongated. Over a period of 30 days, the population of WYEC108 colonized emerging roots of germinating seeds and remained stable (10(5) CFU/g) in the rhizosphere, whereas the nonrhizosphere population of WYEC108 declined at least 100-fold (from 10(5) to 10(3) or fewer CFU/g). The stability of the WYEC108 population incubated at 25 degrees C in the formulation, in sterile soil, and in nonsterile soil was also evaluated. In all three environments, the population of WYEC108 maintained its size for 90 days or more. When pea, cotton, and sweet corn seeds were placed into sterile and nonsterile soils containing 10(6) or more CFU of WYEC108 per g, it colonized the emerging roots. After a 1-week growing period, WYEC108 populations of 10(5) CFU/g (wet weight) of root were found on pea roots in the amended sterile soil environment versus 10(4) CFU/g in amended nonsterile soil. To further study the in vitro interaction between the streptomycete and P. ultimum, mycelia of WYEC108 were mixed with oospores of P. ultimum in agar, which was then used as a film to coat slide coverslips.(ABSTRACT TRUNCATED AT 400 WORDS)
PMCID: PMC167587  PMID: 7487043
13.  Strip-tilled Cover Cropping for Managing Nematodes, Soil Mesoarthropods, and Weeds in a Bitter Melon Agroecosystem 
Journal of Nematology  2010;42(2):111-119.
A field trial was conducted to examine whether strip-tilled cover cropping followed by living mulch practice could suppress root-knot nematode (Meloidogyne incognita) and enhance beneficial nematodes and other soil mesofauna, while suppressing weeds throughout two vegetable cropping seasons. Sunn hemp (SH), Crotalaria juncea, and French marigold (MG), Tagetes patula, were grown for three months, strip-tilled, and bitter melon (Momordica charantia) seedlings were transplanted into the tilled strips; the experiment was conducted twice (Season I and II). Strip-tilled cover cropping with SH prolonged M. incognita suppression in Season I but not in Season II where suppression was counteracted with enhanced crop growth. Sunn hemp also consistently enhanced bacterivorous and fungivorous nematode population densities prior to cash crop planting, prolonged enhancement of the Enrichment Index towards the end of both cash crop cycles, and increased numbers of soil mesoarthropods. Strip-tilled cover cropping of SH followed by clipping of the living mulch as surface mulch also reduced broadleaf weed populations up to 3 to 4 weeks after cash crop planting. However, SH failed to reduce soil disturbance as indicated by the Structure Index. Marigold suppressed M. incognita efficiently when planted immediately following a M. incognita-susceptible crop, but did not enhance beneficial soil mesofauna including free-living nematodes and soil mesoarthropods. Strip-tilled cover cropping of MG reduced broadleaf weed populations prior to cash crop planting in Season II, but this weed suppression did not last beyond the initial cash crop cycle.
PMCID: PMC3380468  PMID: 22736847
Crotalaria juncea; free-living nematodes; living mulch; Meloidogyne incognita; mesoarthropods; Momordica charantia; nematode community analysis; Tagetes patula
14.  The Phytotoxicity Changes of Sewage Sludge-Amended Soils 
Water, Air, and Soil Pollution  2012;223(8):4937-4948.
The aim of the present study was the estimation of changes in the phytotoxicity of soils amended with sewage sludge with relation to Lepidium sativum, Sinapis alba and Sorghum saccharatum. The study was realised in the system of a plot experiment for a period of 29 months. Samples for analyses were taken at the beginning of the experiment, and then after 5, 17 and 29 months. Two kinds of sewage sludge, with varying properties, were added to a sandy soil (soil S) or a loamy soil (soil L) at the dose of 90 t/ha. The addition of sewage sludge to the soils at the start of the experiment caused a significant reduction of both seed germination capacity and root length of the test plants, the toxic effect being distinctly related to the test plant species. With the passage of time the negative effect of sewage sludge weakened, the extent of its reduction depending both of the kind of sewage sludge applied and on the type of soil. Phytotoxicity of the soils amended with the sewage sludges was significantly lower at the end of the experiment than at the beginning. The species of the plants grown on the soils also had a significant effect on their phytotoxicity. The greatest reduction of toxicity was observed in the soil on which no plants were grown (sandy soil) and in the soil under a culture of willow (loamy soil). Solid phase of sewage sludge-amended soils was characterised by higher toxicity than their extracts.
Electronic supplementary material
The online version of this article (doi:10.1007/s11270-012-1248-8) contains supplementary material, which is available to authorized users.
PMCID: PMC3443479  PMID: 23002312
Phytotoxicity; Sewage sludge; Field experiment; Solid phase; Extracts
15.  Effects of selenium accumulation on reproductive functions in Brassica juncea and Stanleya pinnata 
Journal of Experimental Botany  2011;62(15):5633-5640.
Selenium (Se) is an essential micronutrient for many organisms, but is also a toxin and environmental pollutant at elevated levels. Due to its chemical similarity to sulphur, most plants readily take up and assimilate Se. Se accumulators such as Brassica juncea can accumulate Se between 0.01% and 0.1% of dry weight (DW), and Se hyperaccumulators such as Stanleya pinnata (Brassicaeae) contain between 0.1% and 1.5% DW of Se. While Se accumulation offers the plant a variety of ecological benefits, particularly protection from herbivory, its potential costs are still unexplored. This study examines the effects of plant Se levels on reproductive functions. In B. juncea, Se concentrations >0.05–0.1% caused decreases in biomass, pollen germination, individual seed and total seed weight, number of seeds produced, and seed germination. In S. pinnata there was no negative effect of increased Se concentration on pollen germination. In cross-pollination of B. juncea plants with different Se levels, both the maternal and paternal Se level affected reproduction, but the maternal Se concentration had the most pronounced effect. Interestingly, high-Se maternal plants were most efficiently pollinated by Se-treated paternal plants. These data provide novel insights into the potential reproductive costs of Se accumulation, interactive effects of Se in pollen grains and in the pistil, and the apparent evolution of physiological tolerance mechanisms in hyperaccumulators to avoid reproductive repercussions.
PMCID: PMC3223055  PMID: 21841173
Hyperaccumulation; Indian mustard; phytoremediation; pollen germination; prince's plume
16.  Control of Meloidogyne incognita Using Mixtures of Organic Acids 
The Plant Pathology Journal  2014;30(4):450-455.
This study sought to control the root-knot nematode (RKN) Meloidogyne incognita using benign organo-chemicals. Second-stage juveniles (J2) of RKN were exposed to dilutions (1.0%, 0.5%, 0.2%, and 0.1%) of acetic acid (AA), lactic acid (LA), and their mixtures (MX). The nematode bodies were disrupted severely and moderately by vacuolations in 0.5% of MX and single organic acids, respectively, suggesting toxicity of MX may be higher than AA and LA. The mortality of J2 was 100% at all concentrations of AA and MX and only at 1.0% and 0.5% of LA, which lowered slightly at 0.2% and greatly at 0.1% of LA. This suggests the nematicidal activity of MX may be mostly derived from AA together with supplementary LA toxicity. MX was applied to chili pepper plants inoculated with about 1,000 J2, for which root-knot gall formations and plant growths were examined 4 weeks after inoculation. The root gall formation was completely inhibited by 0.5% MX and standard and double concentrations of fosthiazate; and inhibited 92.9% and 57.1% by 0.2% and 0.1% MX, respectively. Shoot height, shoot weight, and root weight were not significantly (P ≤ 0.05) different among all treatments and the untreated and non-inoculated controls. All of these results suggest that the mixture of the organic acids may have a potential to be developed as an eco-friendly nematode control agent that needs to be supported by the more nematode control experiments in fields.
PMCID: PMC4262300  PMID: 25506312
control; Meloidogyne incognita; nematode mortality; organic acids; root gall formation
17.  Integrating Low Water Potential Seed Hydration with Other Treatments to Improve Cold Tolerance 
Annals of Botany  1995;75(1):13-19.
Matriconditioning improved the performance of pepper, tomato, sweet corn, snap bean, table beet, sugar beet and watermelon seeds in early field plantings at suboptimal temperatures (averaged over 10 d after planting) ranging from 12 to 18 °C. Reduction in the time to 50% (T50) emergence in conditioned seeds ranged from 0·6 d in watermelon to 3·3 d in pepper and improvement in emergence from 10% in sugar beet to 30% in table beet. Further improvement in emergence occurred by inclusion of pesticides and/or gibberellin during conditioning. A 4 d conditioning of pepper at 25 °C was superior to 7 d conditioning at 15 °C in seeds germinated at 15 °C on filter paper, but 15 °C conditioning was superior in improving percentage emergence in early field plantings. Tomato seeds conditioned at 15 or 25 °C performed equally well in the field. A 2 d conditioning was superior to 1 d conditioning in improving the performance of supersweet sweet corn cultivars grown in a growth chamber at 10/20 °C. The water uptake rate in the presence of Micro-Cel E during matriconditioning of sweet corn seeds was slower than when the seeds were exposed to the same amount of water in absence of the carrier. Electrolyte leakage was greater in supersweet ‘Challenger’ sweet corn seeds carrying the sh2 gene compared to the sugary type sweet corn ‘More’, and in both cases matriconditioning reduced the leakage. Lettuce seeds matriconditioned for 24 h had higher 1-aminocyclopropane-l-carboxylic acid (ACC) content, developed greater ACC oxidase activity and performed better at 10 °C (germinated earlier and had higher percentage germination) than the untreated seeds. Matriconditioning appears to bring about beneficial physical, physiological and biochemical changes that seemingly improve embryo growth potential and tolerance to low temperatures.
PMCID: PMC3023659  PMID: 21247908
Seed matriconditioning; seed osmoconditioning; imbibitional chilling injury; water uptake; solute leakage; vegetable seeds
18.  Cotton Root Protection from Plant-Parasitic Nematodes by Abamectin-Treated Seed 
Journal of nematology  2007;39(1):27-30.
Abamectin is nematicidal to Meloidogyne incognita and Rotylenchulus reniformis, but the duration and length of cotton taproot protection from nematode infection by abamectin-treated seed is unknown. Based on the position of initial root-gall formation along the developing taproot from 21 to 35 d after planting, infection by M. incognita was reduced by abamectin seed treatment. Penetration of developing taproots by both nematode species was suppressed at taproot length of 5 cm by abamectin-treated seed, but root penetration increased rapidly with taproot development. Based on an assay of nematode mobility to measure abamectin toxicity, the mortality of M. incognita associated with a 2-d-old emerging cotton radicle was lower than mortality associated with the seed coat, indicating that more abamectin was on the seed coat than on the radicle. Thus, the limited protection of early stage root development suggested that only a small portion of abamectin applied to the seed was transferred to the developing root system.
PMCID: PMC2586483  PMID: 19259471
abamectin; avermectin; cotton; Gossypium hirsutum; Meloidogyne incognita; nematicide; reniform nematode; root-knot nematode; Rotylenchulus reniformis; seed treatment
19.  Germination of photoblastic lettuce seeds is regulated via the control of endogenous physiologically active gibberellin content, rather than of gibberellin responsiveness 
Journal of Experimental Botany  2008;59(12):3383-3393.
Phytochrome regulates lettuce (Lactuca sativa L. cv. Grand Rapids) seed germination via the control of the endogenous level of bioactive gibberellin (GA). In addition to the previously identified LsGA20ox1, LsGA20ox2, LsGA3ox1, LsGA3ox2, LsGA2ox1, and LsGA2ox2, five cDNAs were isolated from lettuce seeds: LsCPS, LsKS, LsKO1, LsKO2, and LsKAO. Using an Escherichia coli expression system and functional assays, it is shown that LsCPS and LsKS encode ent-copalyl diphosphate synthase and ent-kaurene synthase, respectively. Using a Pichia pastoris system, it was found that LsKO1 and LsKO2 encode ent-kaurene oxidases and LsKAO encodes ent-kaurenoic acid oxidase. A comprehensive expression analysis of GA metabolism genes using the quantitative reverse transcription polymerase chain reaction suggested that transcripts of LsGA3ox1 and LsGA3ox2, both of which encode GA 3-oxidase for GA activation, were primarily expressed in the hypocotyl end of lettuce seeds, were expressed at much lower levels than the other genes tested, and were potently up-regulated by phytochrome. Furthermore, LsDELLA1 and LsDELLA2 cDNAs that encode DELLA proteins, which act as negative regulators in the GA signalling pathway, were isolated from lettuce seeds. The transcript levels of these two genes were little affected by light. Lettuce seeds in which de novo GA biosynthesis was suppressed responded almost identically to exogenously applied GA, irrespective of the light conditions, suggesting that GA responsiveness is not significantly affected by light in lettuce seeds. It is proposed that lettuce seed germination is regulated mainly via the control of the endogenous content of bioactive GA, rather than the control of GA responsiveness.
PMCID: PMC2529229  PMID: 18653696
Germination; gibberellin metabolism; gibberellin signalling; lettuce
20.  Evaluation of the Effect of Ecologic on Root Knot Nematode, Meloidogyne incognita, and Tomato Plant, Lycopersicon esculenum 
Nonchemical methods and strategies for nematode management including cultural methods and engineered measures have been recommended as an alternative to methyl bromide (a major soil fumigant), due to its role in the depletion of the ozone layer. Hence, an international agreement has recently been reached calling for its reduced consumption and complete phasing out. This present research evaluates the potential of Ecologic, a biological, marine shell meal chitin material, as a soil amendment management agent for root knot nematode, Meloidogyne incognita, control, and its effect on the growth of Floradel tomato plant, Lycopersicon esculentum. To accomplish this goal, studies were conducted during which, experimental pots were set up in greenhouse environments using sterilized soil inoculated with 5,000 root-knot eggs per 1500 g soil. There were 4 treatments and 5 replications. Treatments were: No chitin; 50 g chitin; 100 g chitin; and 200 g chitin. A two-week wait period following Ecologic amendment preceded Floradel tomato planting to allow breakdown of the chitin material into the soil. Fresh and dry weights of shoot and root materials were taken as growth end-points. A statistically significant difference (p ≤ 0.05) was obtained with regard to the growth rate of L. esculentum at 100 g chitin treatment compared to the control with no chitin. Mean fresh weights of Floradel tomato were 78.0 ± 22.3g, 81.0 ± 20.3g, 109.0 ± 25.4g and 102.0 ± 33.3g at 0, 50, 100 and 200g chitin, respectively. The analysis of root knot nematode concentrations indicated a substantial effect on reproduction rate associated with chitin amendment. Study results showed a significant decrease in both root knot nematode eggs and juveniles (J2) at 100g and 200g Ecologic chitin levels, however, an increase in nematode concentrations was recorded at the 50g Ecologic chitin level (p ≤ 0.05). The mean amounts of J2 population, as expressed per 1500cm3 soil, were 49,933 ± 38,819, 86,050 ± 25248, 103 ± 133 and 103 ± 133 for 0, 50, 100 and 200g chitin, respectively. Similarly, the mean numbers of root knot nematode eggs (per 1500cm3 of soil) were 40,759 ± 36,712, 66,048 ± 39,730, 9,904 ± 16,591 and 9,257 ± 17,204. Root gall rating was also significantly lower (p ≤ 0.05) at the 100g and 200g chitin levels compared to the control. Percent gall ratings were 3.3 ± 1.0%, 3.2 ± 1.0%, 1.0 ± 0.5%, and 1.0% ± 0.6% for amendment levels of 0, 50, 100, and 200g chitin, respectively.
PMCID: PMC3662226  PMID: 18678924
Ecologic chitin; root knot nematode; tomato plant; agricultural management
21.  Mixtures of Olive Pomace with Different Nitrogen Sources for the Control of Meloidogyne spp. on Tomato 
Journal of Nematology  1995;27(4S):575-584.
The efficacy of mixtures of dry olive (Olea europea) pomace with biuret, guanidine, and melamine for control of root-knot nematodes (Meloidogyne spp.) on tomato (Lycopersicon esculentum) was studied in greenhouse experiments. Olive pomace (OP) applied pre-plant at 10 g/kg soil was phytotoxic. Mixtures of OP (10 g/kg soil) with biuret or guanidine at 200-300 mg/kg soil reduced or eliminated the phytotoxic effect, controlled root-knot nematodes, and increased soil esterase activity indicative of microbial activity. The addition of biuret or guanidine without OP to soil at rates <300 mg/kg soil did not control root-knot nematodes. Melamine applied at 100-400 mg/kg soil was phytotoxic as were mixtures of melamine with OP. Treatment of OP with anhydrous ammonia increased N content of the material. In another greenhouse experiment, NH₃-treated OP added to soil was not phytotoxic to tomato, suppressed root-knot nematodes, and increased soil esterase activity. Greenhouse and microplot experiments with OP plus chicken litter demonstrated the efficacy of these combination amendments to control root-knot nematodes and increase tomato yields in Meloidogyne-infested soil.
PMCID: PMC2619652  PMID: 19277325
amendments; anhydrous ammonia; biuret; chicken litter; control; guanidine; melamine; Meloidogyne spp.; nematode; Olea europaea; olive pomace; tomato; root-knot nematode
22.  Effect of Broccoli (Brassica oleracea) Tissue, Incorporated at Different Depths in a Soil Column, on Meloidogyne incognita  
Journal of nematology  2007;39(2):111-117.
Brassicas have been used frequently for biofumigation, a pest-management strategy based on the release of biocidal volatiles during decomposition of soil-incorporated tissue. However, the role of such volatiles in control of plant-parasitic nematodes is unclear. The goal of this study was to determine the direct localized and indirect volatile effects of amending soil with broccoli tissue on root-knot nematode populations. Meloidogyne incognita-infested soil in 50-cm-long tubes was amended with broccoli tissue, which was mixed throughout the tube or concentrated in a 10-cm layer. After three weeks at 28°C, M. incognita populations in the amended tubes were 57 to 80% smaller than in non-amended tubes. Mixing broccoli throughout the tubes reduced M. incognita more than concentrating broccoli in a 10-cm layer. Amending a 10-cm layer reduced M. incognita in the non-amended layers of those tubes by 31 to 71%, probably due to a nematicidal effect of released volatiles. However, the localized direct effect was much stronger than the indirect effect of volatiles. The strong direct effect may have resulted from the release of non-volatile nematicidal compounds. Therefore, when using biofumigation with broccoli to control M. incognita, the tissue should be thoroughly and evenly mixed through the soil layer(s) where the target nematodes occur. Effects on saprophytic nematodes were the reverse. Amended soil layers had much greater numbers of saprophytic nematodes than non-amended layers, and there was no indirect effect of amendments on saprophytic nematodes in adjacent non-amended layers.
PMCID: PMC2586485  PMID: 19259479
amendment; biofumigation; broccoli; Brassica oleracea; management; Meloidogyne incognita; root-knot nematode; soil
23.  Evaluation of Several Approaches to Manage Meloidogyne incognita and Cotton Seedling Disease Complexes in the High Plains of Texas 
Journal of Nematology  2005;37(1):66-70.
Field experiments were conducted for control of the southern root-knot nematode (Meloidogyne incognita) and cotton seedling disease fungi (primarily Thielaviopsis basicola) in one naturally infested field during 1999 and 2000 and in three additional fields in 2000. Treatments included: seed-applied fungicides (triadimenol + mefenoxam + thiram and carboxin + PCNB + mefenoxam), cultivars (Paymaster [PM] 2326 RR and PM 2200 RR), and a nematicide (aldicarb at 0.83 kg a.i/ha). Plant stands were higher (P = 0.02) in the presence of aldicarb (77% emergence) than in its absence (74% emergence). Hypocotyl disease symptom ratings were lower (P = 0.0001) following triadimenol + mefenoxam + thiram seed treatment (0.53) as compared with carboxin + PCNB + mefenoxam (0.93). Root necrosis was lower (P = 0.002) following triadimenol + mefenoxam + thiram seed treatment (27%) as compared with carboxin + PCNB + mefenoxam (34%). In one field, in both years, aldicarb was associated with more root necrosis (58%) than in its absence (46%) (P = 0.004). At three other sites aldicarb did not affect root necrosis. Population densities of Meloidogyne incognita eggs and juveniles at midseason were greater (P = 0.005, P = 0.003, respectively) on PM 2200 RR (less resistant) than on PM 2326 RR (more resistant). Yield was affected by the plant genotype by aldicarb interaction (P = 0.02) but not by seed treatments. Aldicarb effect on yield was dependent on cultivar, whereas affect of seed treatment on root health was consistent and independent of cultivar and aldicarb. No conditions were identified when use of triadimenol + mefenoxam was detrimental.
PMCID: PMC2620942  PMID: 19262844
aldicarb; cotton; fungicides; Meloidogyne incognita; root-knot nematode; seedling disease; Thielaviopsis basicola
24.  Effect of Combining Soil Solarization with Certain Nematicides on Target and Nontarget Organisms and Plant Growth 
Journal of Nematology  1987;19(Annals 1):107-112.
Field experiments compared pesticidal and plant growth effects of soil solarization, alone and in combination, with overall applications of several nematicides. Nematodes, including Meloidogyne incognita J2, that were targeted for control were significantly reduced (P < 0.05) by solarization, 1,3-dichloropropene (44 and 132 liter/ha), ethoprop (13.5 kg/ha), metham sodium (64 liter/ha), formaldehyde (111 liter/ha), and by solarization-nematicide combinations. Control of Pythium ultimum also was obtained by all of the treatments; however, none of the chemicals or combinations of chemicals and solarization controlled nematodes or P. ultimum significantly better than solarization alone. Numbers of cotton (Gossypium hirsutum cv. Acala SJ-2) seed-applied Trichoderma viride and Bacillus subtilis which colonized the plant rhizosphere were not affected. Yield of carrot and survival of cotton seedlings was sometimes increased by solarization and (or) chemical treatments. No significant phytotoxicity from soil treatments was found on cotton or carrot.
PMCID: PMC2618686  PMID: 19290287
Bacillus subtilis; biological control; chemical control; Criconemella xenoplax; Daucus carota; 1,3-dichloropropene; ethoprop; formaldehyde; Gossypium hirsutum; Meloidogyne incognita; metham sodium; Pythium ultimum; ring nematode; solarization; southern root-knot nematode; Trichoderma viride
25.  Influence of 1,3-Dichloropropene, Fenamiphos, and Carbofuran on Meloidogyne incognita Populations and Yield of Chile Peppers 
Journal of Nematology  1994;26(4S):683-689.
Field trials were conducted during 1986, 1988, 1989, and 1991 to compare the effects of 1,3-dichloropropene, fenamiphos, and carbofuran on yield and quality of chile peppers (Capsicum annuum) in soil infested with Meloidogyne incognita. When compared with untreated plots, numbers of M. incognita juveniles recovered from soil 60 and(or) 90 days after chile pepper emergence were reduced (P = 0.05) following 1,3-D treatment every year except 1986. Nematode numbers were also reduced (P = 0.05) by fenamiphos in 1989. Chile pepper yields were significantly higher than those in untreated control plots (P = 0.05) all 4 years in plots treated with 1,3-D and in 1989 in plots treated with fenamiphos. Use of carbofuran did not significantly reduce nematode numbers or enhance yields in these experiments. Green chile pepper fruit quality was enhanced (P = 0.05) following 1,3-D treatments in 1988 and 1989 but was unaffected by fenamiphos or carbofuran application. Increasing placement depth of 1,3-D from 28 to 48 cm increased (P = 0.05) red chile pepper yield compared with that obtained with conventional placement in 1988 only, and did not affect green chile pepper yield.
PMCID: PMC2619573  PMID: 19279948
application technique; Capsicum annuum; carbofuran; chile pepper; crop quality; depth of placement; fenamiphos; fumigation; Meloidogyne incognita; nematode; 1,3-dichloropropene; root-knot nematode; yield

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