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1.  Proceedings of The 8th Romanian National HIV/AIDS Congress and The 3rd Central European HIV Forum 
Alexiev, Ivailo | Dimitrova, Reneta | Gancheva, Anna | Kostadinova, Asya | Stoycheva, Mariyana | Nikolova, Daniela | Elenkov, Ivaylo | Tilișcan, Cătălin | Predescu, Mioara | Păunescu, Bogdan | Streinu-Cercel, Anca | Săndulescu, Oana | Șchiopu, Claudiu Mihai | Hristache, Mădălina | Brîndușe, Lăcrămioara Aurelia | Streinu-Cercel, Adrian | Todorovic, Marija | Siljic, Marina | Salemovic, Dubravka | Nikolic, Valentina | Pesic-Pavlovic, Ivana | Ranin, Jovan | Jevtovic, Djordje | Stanojevic, Maja | Tudor, Ana Maria | Vlad, Delia | Mărdărescu, Mariana | Petrea, Sorin | Petre, Cristina | Neagu-Drăghicenoiu, Ruxandra | Ungurianu, Rodica | Cibea, Alina | Chirilă, Odette | Anghelina, Cristian | Coserea, Ileana | Krikelli, Pantelia-Amalia | Pavlitina, Eirini | Psichogiou, Mina | Lamnisos, Demetris | Williams, Leslie | Korobchuk, Anya | Skaathun, Britt | Smyrnov, Pavlo | Schneider, John | Sypsa, Vana | Paraskevis, Dimitrios | Hatzakis, Angelos | Friedman, Samuel R. | Nikolopoulos, Georgios K. | Dragović, Gordana | Srdić, Danica | Khawla, Al Musalhi | Soldatović, Ivan | Nikolić, Jelena | Jevtović, Djordje | Nair, Devaki | Temereanca, Aura | Rosca, Adelina | Ene, Luminita | Soontornniyomkij, Benchawa | Diaconu, Carmen | Dita, Claudia | Achim, Cristian | Ruta, Simona | Benea, Șerban | Moroti, Ruxandra | Jipa, Raluca | Manea, Eliza | Stan, Andrada | Benea, Elisabeta | Oțelea, Dan | Hristea, Adriana | Hristea, Adriana | Lăpădat, Irina | Jipa, Raluca | Moroti, Ruxandra | Benea, Șerban | Antonică, Doina | Panait, Irina | Petre, Roxana | Kowalska, Justyna D. | Pietraszkiewicz, Ewa | Grycner, Ewa | Firlag-Burkacka, Ewa | Horban, Andrzej | Vlaicu, Ovidiu | Bănică, Leontina | Paraschiv, Simona | Tudor, Ana-Maria | Moroti, Ruxandra | Oțelea, Dan | Dimitrijević, Bojana | Soldatović, Ivan | Jevtović, Đorđe | Kusić, Jovana | Salemović, Dubravka | Ranin, Jovan | Dragović, Gordana | Florea, Dragoș | Bădicuț, Ioana | Rafila, Alexandru | Camburu, Cornel | Histrea, Adriana | Frățilă, Mihaela | Oțelea, Dan | Gmizic, Ivana | Salemovic, Dubravka | Pesic-Pavlovic, Ivana | Siljic, Marina | Nikolic, Valentina | Djonin-Nenezic, Miljana | Milosevic, Ivana | Brmbolic, Branko | Stanojevic, Maja | Streinu-Cercel, Anca | Săndulescu, Oana | Neguț, Alina Cristina | Predescu, Mioara | Mărdărescu, Alexandra | Săndulescu, Mihai | Streinu-Cercel, Adrian | Pérez, Ana Belen | Chueca, Natalia | Álvarez, Marta | Alados, Juan Carlos | Rivero, Antonio | Vera, Francisco | Delgado, Marcial | Salmeron, Javier | Jiménez, Miguel | Blanco, Maria José | Diago, Moises | Garcia-deltoro, Miguel | Alvarez, Marta | Téllez, Francisco | García, Federico | Tănase, Diana | Manea, Eliza | Bacruban, Rodica | Florea, Dragoș | Oțelea, Dan | Rafila, Alexandru | Mărdărescu, Mariana | Hristea, Adriana | Grgic, Ivana | Planinic, Ana | Santak, Maja | Gorenec, Lana | Lepej, Snjezana Zidovec | Vince, Adriana | Manea, Eliza | Hristea, Adriana | Benea, Șerban | Moroti, Ruxandra | Tănase, Diana | Niculae, Cristian M. | Merisor, Simona | Jipa, Raluca | Paraskevis, Dimitrios | Kostaki, Evangelia | Nikolopoulos, Georgios K. | Sypsa, Vana | Psichogiou, Mina | Paraskeva, Dimitra | Skoutelis, Athanassios | Malliori, Meni | Friedman, Samuel R. | Hatzakis, Angelos | Hackiewicz, Malgorzata | Zabek, Piotr | Firlag-Burkacka, Ewa | Horban, Andrzej | Kowalska, Justyna Dominika | Lunar, Maja M. | Mlakar, Jana | Poljak, Mario | Bănică, Leontina | Martin, Eliza | Gheorghiță, Valeriu | Petrescu, Andrei | Oțelea, Dan | Popescu, Costin-Ioan | Paraschiv, Simona | Neaga, Emil | Ovidiu, Vlaicu | Juncu, Andrei | Bănică, Leontina | Paraschiv, Simona | Oțelea, Dan | Popescu, Costin-Ioan | Luca, Adrian | Lazăr, Florin | Luca, Anca Elena | Ene, Luminița | Achim, Cristian | Gingăraş, Cosmina | Anton, Ștefan Adrian | Rădoi, Roxana | Tetradov, Simona | Țârdei, Grațiela | Nica, Maria | Capşa, Razvan Alexandru | Achim, Cristian L. | Oprea, Cristiana | Ene, Luminița | Szymańska, Bogna | Gawron, Natalia | Pluta, Agnieszka | Łojek, Emilia | Firląg-Burkacka, Ewa | Horban, Andrzej | Bornstein, Robert | Burcoș, Olivia | Erscoiu, Simona Manuela | Cojanu, Filofteia Bănicioiu | Toderan, Andreea | Nica, Maria | Popa, Ionuț Cristian | Ceaușu, Emanoil | Calistru, Petre Iacob | Arbune, Manuela | Alexandrache, Mirela | Arbune, Anca-Adriana | Voinescu, Doina-Carina | Diaconu, Ioan-Alexandru | Stratan, Laurențiu | Aramă, Victoria | Nichita, Luciana | Diaconu, Alexandra | Negru, Anca | Orfanu, Alina | Leuștean, Anca | Ion, Daniela Adriana | Ianache, Irina | Oprea, Cristiana | Leuștean, Anca | Popescu, Cristina | Orfanu, Alina | Negru, Anca | Catana, Remulus | Murariu, Cristina | Diaconu, Ioan-Alexandru | Rădulescu, Mihaela | Tilișcan, Cătălin | Aramă, Victoria | Marincu, Iosif | Poptelecan, Patricia | Bică, Valeria | Lazăr, Florin | Tirnea, Livius | Ianache, Irina | Rădoi, Roxana | Nica, Manuela | Țârdei, Grațiela | Ene, Luminița | Ceaușu, Emanoil | Calistru, Petre | Oprea, Cristiana | Osoianu, Iurie | Halacu, Ala | Stoian, Andreea Cristina | Dumitrescu, Florentina | Diaconescu, Iulian | Cupșa, Augustin | Giubelan, Lucian | Ionescu, Loredana | Niculescu, Irina | Chiriac, Carmen | Șincu, Nina | Kezdi, Iringo Zaharia | Georgescu, Anca | Țilea, Brândușa | Girbovan, Cristina | Incze, Andrea | Fodor, Andrea | Cibea, Alina | Mărdărescu, Mariana | Petre, Cristina | Drăghicenoiu, Ruxandra | Ungurianu, Rodica | Tudor, Ana Maria | Vlad, Delia | Matei, Carina | Dumea, Elena | Petcu, Lucian Cristian | Cambrea, Simona Claudia | Dumitrescu, Florentina | Cupsa, Augustin | Stoian, Andreea Cristina | Giubelan, Lucian | Niculescu, Irina | Diaconescu, Iulian | Hurezeanu, Dan | Dragonu, Livia | Cotulbea, Mioara | Erscoiu, Simona Manuela | Popa, Ionuț Cristian | Stroie, Denisa | Ionescu, Petronela | Duță, Nedeea | Dobrea, Camelia | Voican, Irina | Ceaușu, Emanoil | Calistru, Petre Iacob | Lazăr, Florin | Giubelan, Lucian | Cupșa, Augustin | Diaconescu, Iulian | Dumitrescu, Florentina | Hurezeanu, Dan | Dragonu, Livia | Niculescu, Irina | Stoian, Andreea Cristina | Obretin, Oana | Stănescu, Mariana | Jianu, Mihai | Gorenec, Lana | Lepej, Snjezana Zidovec | Grgic, Ivana | Planinic, Ana | Bes, Janja Iscic | Vince, Adriana | Begovac, Josip | Horga, Luminița Elena | Itu, Corina | Horga, Luminița Elena | David-Aldea, Laura Augusta | Ciorogar, Anca | Jianu, Cristian | Lupșe, Mihaela | Caramangiu, Iuliana | Roșca, Ovidiu | Cialma, Monica | Ardeleanu, Andreea | Marincu, Iosif | Jipa, Raluca | Manea, Eliza | Benea, Șerban | Lăpădat, Irina | Irimescu, Nicoleta | Panait, Irina | Niculae, Cristian | Hristea, Adriana | Kusic, Jovana | Jevtovic, Djordje | Salemovic, Dubravka | Ranin, Jovan | Dimitrijevic, Bozana | Dragovic, Gordana | Aldea-David, Laura-Augusta | Manciuc, Carmen | Nicolau, Cristina | Prisăcariu, Liviu | Largu, Alexandra | Mărdărescu, Mariana | Streinu-Cercel, Adrian | Petre, Cristina | Iancu, Marieta | Vintilă, Sanda | Vitelaru, Daniela | Ionel, Iosif | Șchiopu, Claudiu Mihai | Mărdărescu, Alexandra-Henriette | Micsanschi, Pavel | Holban, Tiberiu | Bîstrițchi, Ina | Pârțână, Lucia | Nagîț, Angela | Popovici, Svetlana | Talmaci, Maria | Cucerova, Irina | Mitrescu, Sorina Georgiana | Mihalcea, Dana | Caramangiu, Iulia | Roșca, Ovidiu | Maricu, Iosif | Negru, Anca | Munteanu, Daniela | Aramă, Victoria | Mihăilescu, Raluca | Diaconu, Ioan | Catana, Remulus | Popescu, Cristina | Orfanu, Alina | Leuștean, Anca | Rădulescu, Mihaela | Tilișcan, Cătălin | Năstase, Raluca | Molagic, Violeta | Duport, Irina | Dragomirescu, Cristina | Aramă, Ștefan Sorin | Negruț, Nicoleta M. | Niță, Violeta Elena | Munteanu, Daniela Ioana | Mihăilescu, Raluca | Diaconu, Ioan | Negru, Anca | Popescu, Cristina | Aramă, Victoria | Orfanu, Alina | Popescu, Cristina | Leuștean, Anca | Negru, Anca | Catana, Remulus | Diaconu, Ioan | Tilișcan, Cătălin | Aramă, Victoria | Aramă, Sorin Ștefan | Pavlovia, Ivana Pesic | Salemovic, Dubravka | Ranin, Jovan | Jevtovic, Djordje | Roșca, Ovidiu | Ardeleanu, Andreea | Caramangiu, Iulia | Desaga, Daniela | Bică, Valerica | Mitrescu, Sorina | Marincu, Iosif | Siljic, Marina | Salemovic, Dubravka | Nikolic, Valentina | Jevtovic, Djordje | Pesic-Pavlovic, Ivana | Ranin, Jovan | Todorovic, Marija | Stanojevic, Maja | Șincu, Nina-Ioana | Georgescu, Anca | Țilea, Brândușa | Kezdi, Iringo Zaharia | Incze, Andrea | Gârbovan, Cristina | Chiriac, Carmen Lucia | Luca, Anca Elena | Lazăr, Florin | Luca, Adrian | Ene, Luminița | Rădoi, Roxana | Talnariu, Adina | Suciu, Silvia | Achim, Cristian | Iacob, Diana Gabriela | Florea, Dragoș | Iacob, Simona | Arbune, Manuela | Drăgănescu, Miruna | Iancu, Alina | Moroti, Ruxandra | Niculae, Cristian M. | Merisor, Simona | Manea, Eliza | Benea, Serban | Stan, Andrada | Hrisca, Raluca | Jipa, Raluca | Tanase, Diana | Hristea, Adriana | Grgic, Ivana | Planinic, Ana | Gorenec, Lana | Lepej, Snjezana Zidovec | Vince, Adriana
BMC Infectious Diseases  2016;16(Suppl 3):290.
O1 HIV-1 diversity in Bulgaria (current molecular epidemiological picture)
Ivailo Alexiev, Reneta Dimitrova, Anna Gancheva, Asya Kostadinova, Mariyana Stoycheva, Daniela Nikolova, Ivaylo Elenkov
O2 Knowledge, attitudes and practices of the general population on HIV/AIDS, hepatitis B and C in Romania
Cătălin Tilișcan, Mioara Predescu, Bogdan Păunescu, Anca Streinu-Cercel, Oana Săndulescu, Claudiu Mihai Șchiopu, Mădălina Hristache, Lăcrămioara Aurelia Brîndușe, Adrian Streinu-Cercel
O3 The prevalence of human leukocyte antigen-B*57:01 allele carriers and CXCR4 tropism among newly diagnosed HIV infected patients in Serbia
Marija Todorovic, Marina Siljic, Dubravka Salemovic, Valentina Nikolic, Ivana Pesic-Pavlovic, Jovan Ranin, Djordje Jevtovic, Maja Stanojevic
O4 HIV transmission among stable serodiscordant couples from the former Pediatric Cohort follow up in the National Institute of Infectious Diseases
Ana Maria Tudor, Delia Vlad, Mariana Mărdărescu, Sorin Petrea, Cristina Petre, Ruxandra Neagu-Drăghicenoiu, Rodica Ungurianu, Alina Cibea, Odette Chirilă, Cristian Anghelina, Ileana Coserea
O5 Unemployment is associated with syringe sharing among people who inject drugs in Greece
Pantelia-Amalia Krikelli, Eirini Pavlitina, Mina Psichogiou, Demetris Lamnisos, Leslie Williams, Anya Korobchuk, Britt Skaathun, Pavlo Smyrnov, John Schneider, Vana Sypsa, Dimitrios Paraskevis, Angelos Hatzakis, Samuel R. Friedman, Georgios K. Nikolopoulos
O6 Correlation of adipocytokine levels in different types of lipodystrophy in HIV/AIDS patients
Gordana Dragović, Danica Srdić, Al Musalhi Khawla, Ivan Soldatović, Jelena Nikolić, Djordje Jevtović, Devaki Nair
O7 IP10 – a possible biomarker for the progression of HIV infection
Aura Temereanca, Adelina Rosca, Luminita Ene, Benchawa Soontornniyomkij, Carmen Diaconu, Claudia Dita, Cristian Achim, Simona Ruta
O8 A permanent challenge: persistent low viremia in HIV positive patients on ART
Șerban Benea, Ruxandra Moroti, Raluca Jipa, Eliza Manea, Andrada Stan, Elisabeta Benea, Dan Oțelea, Adriana Hristea
O9 Infections in IDUs according to their HIV status
Adriana Hristea, Irina Lăpădat, Raluca Jipa, Ruxandra Moroti, Șerban Benea, Doina Antonică, Irina Panait, Roxana Petre
O10 Trends in combined antiretroviral therapy used in methadone program integrated with HIV care - 20 years of experience
Justyna D. Kowalska, Ewa Pietraszkiewicz, Ewa Grycner, Ewa Firlag-Burkacka, Andrzej Horban
O11 Extracellular cyclophilin A – inflammatory mediator in HIV infected patients
Ovidiu Vlaicu, Leontina Bănică, Simona Paraschiv, Ana-Maria Tudor, Ruxandra Moroti, Dan Oțelea
O12 High cardiovascular disease risk in Serbian population, an issue of concern
Bojana Dimitrijević, Ivan Soldatović, Đorđe Jevtović, Jovana Kusić, Dubravka Salemović, Jovan Ranin, Gordana Dragović
O13 Genotypic rifampicin resistance in HIV/ tuberculosis coinfected patients from a tertiary level infectious diseases hospital
Dragoș Florea, Ioana Bădicuț, Alexandru Rafila, Cornel Camburu, Adriana Histrea, Mihaela Frățilă, Dan Oțelea
O14 Occurrence of residual HCV RNA in liver and peripheral blood mononuclear cells among patients with chronic hepatitis C infection and/or HCV/HIV coinfection after IFN-based therapy
Ivana Gmizic, Dubravka Salemovic, Ivana Pesic-Pavlovic, Marina Siljic, Valentina Nikolic, Miljana Djonin-Nenezic, Ivana Milosevic, Branko Brmbolic, Maja Stanojevic
O15 Romanian nationwide screening for infection with HIV and hepatitis B and C viruses
Anca Streinu-Cercel, Oana Săndulescu, Alina Cristina Neguț, Mioara Predescu, Alexandra Mărdărescu, Mihai Săndulescu, Adrian Streinu-Cercel
O16 Treatment emergent variants to combined direct antiviral agents therapy against hepatitis C virus
Ana Belen Pérez, Natalia Chueca, Marta Álvarez, Juan Carlos Alados, Antonio Rivero, Francisco Vera, Marcial Delgado, Javier Salmeron, Miguel Jiménez, Maria José Blanco, Moises Diago, Miguel Garcia-deltoro, Marta Alvarez, Francisco Téllez, Federico García
O17 Clinical and epidemiological aspects in tuberculosis/HIV coinfected patients
Diana Tănase, Eliza Manea, Rodica Bacruban, Dragoș Florea, Dan Oțelea, Alexandru Rafila, Mariana Mărdărescu, Adriana Hristea
O18 Resistance to NS3 protease inhibitors in persons with chronic hepatitis C infected with hepatitis C virus subtype 1a from Croatia
Ivana Grgic, Ana Planinic, Maja Santak, Lana Gorenec, Snjezana Zidovec Lepej, Adriana Vince
O19 Analysis of a simplified diagnostic score for tuberculous meningitis in HIV-infected adults with meningitis
Eliza Manea, Adriana Hristea, Șerban Benea, Ruxandra Moroti, Diana Tănase, Cristian M. Niculae, Simona Merisor, Raluca Jipa
O20 Molecular tracing of the origin of HIV-1 infection among persons who inject drugs in Athens: a phyloethnic study
Dimitrios Paraskevis, Evangelia Kostaki, Georgios K. Nikolopoulos, Vana Sypsa, Mina Psichogiou, Dimitra Paraskeva, Athanassios Skoutelis, Meni Malliori, Samuel R. Friedman, Angelos Hatzakis
O21 The dynamics of virological response to HIV-1 infection and antiretroviral therapy initiation in patients with and without HLA-B*5701 Allele
Malgorzata Hackiewicz, Piotr Zabek, Ewa Firlag-Burkacka, Andrzej Horban, Justyna Dominika Kowalska
O22 Increase in the numbers of non-B subtypes and potential recombinant forms circulating among Slovenian MSM in the recent years
Maja M. Lunar, Jana Mlakar, Mario Poljak
O23 Genotyping intrahost polymorphisms in hepatitis C virus E2 protein associated with resistance to antibody neutralization
Leontina Bănică, Eliza Martin, Valeriu Gheorghiță, Andrei Petrescu, Dan Oțelea, Costin-Ioan Popescu, Simona Paraschiv
O24 Genotyping of HCV NS3 protease inhibitors resistance and phenotyping of rare double resistance mutations in HCV cell culture system
Emil Neaga, Vlaicu Ovidiu, Andrei Juncu, Leontina Bănică, Simona Paraschiv, Dan Oțelea, Costin-Ioan Popescu
O25 Employment status controls the relationship between neurocognitive impairment and depression in a cohort of young HIV-infected adults since childhood
Adrian Luca, Florin Lazăr, Anca Elena Luca, Luminița Ene, Cristian Achim
O26 Predictors of survival in parenterally-infected HIV positive children and youth diagnosed with progressive multifocal leukoencephalopathy
Cosmina Gingăraş, Ștefan Adrian Anton, Roxana Rădoi, Simona Tetradov, Grațiela Țârdei, Maria Nica, Razvan Alexandru Capşa, Cristian L. Achim, Cristiana Oprea, Luminița Ene
O27 Neurocognitive and brain functioning in HIV-infected young MSM treated with cART
Bogna Szymańska, Natalia Gawron, Agnieszka Pluta, Emilia Łojek, Ewa Firląg – Burkacka, Andrzej Horban, Robert Bornstein, et HARMONIA3 Study Group
O28 Clinical value of RT-PCR detection of Toxoplasma gondii DNA in cerebrospinal fluid
Olivia Burcoș, Simona Manuela Erscoiu, Filofteia Bănicioiu Cojanu, Andreea Toderan, Maria Nica, Ionuț Cristian Popa, Emanoil Ceaușu, Petre Iacob Calistru
O29 Characteristics of sleep disorders in Romanian adults infected with human immunodeficiency virus
Manuela Arbune, Mirela Alexandrache, Anca-Adriana Arbune, Doina-Carina Voinescu
O30 Diagnosing neuroHIV: the rift between clinicians and pathologists
Ioan-Alexandru Diaconu, Laurențiu Stratan, Victoria Aramă, Luciana Nichita, Alexandra Diaconu, Anca Negru, Alina Orfanu, Anca Leuștean, Daniela Adriana Ion
O31 A challenging neurological complication in a HIV-infected young woman with multiple opportunistic infections
Irina Ianache, Cristiana Oprea
O32 Brain abscess with uncertain etiology in a late-presenter HIV infected patient
Anca Leuștean, Cristina Popescu, Alina Orfanu, Anca Negru, Remulus Catana, Cristina Murariu, Ioan-Alexandru Diaconu, Mihaela Rădulescu, Cătălin Tilișcan, Victoria Aramă
O33 Cerebral toxoplasmosis and left crural monoparesis with fatal evolution in a noncompliant patient with AIDS C3
Iosif Marincu, Patricia Poptelecan, Valeria Bică, Florin Lazăr, Livius Tirnea
O34 Opportunistic infections still a problem in HIV-infected patients in cART era: a Romanian single center experience
Irina Ianache, Roxana Rădoi, Manuela Nica, Grațiela Țârdei, Luminița Ene, Emanoil Ceaușu, Petre Calistru, Cristiana Oprea
P1: Epidemiological aspects of co-infection of HIV/TB in Moldova
Iurie Osoianu, Ala Halacu
P2 Perinatal exposure at HIV infection in Oltenia region
Andreea Cristina Stoian, Florentina Dumitrescu, Iulian Diaconescu, Augustin Cupșa, Lucian Giubelan, Loredana Ionescu, Irina Niculescu
P3 Women living with HIV in Mureș county
Carmen Chiriac, Nina Șincu, Iringo Zaharia Kezdi, Anca Georgescu, Brândușa Țilea, Cristina Girbovan, Andrea Incze, Andrea Fodor
P4 Late diagnosis of HIV infection in children - a challenge for Romania
Alina Cibea, Mariana Mărdărescu, Cristina Petre, Ruxandra Drăghicenoiu, Rodica Ungurianu, Ana Maria Tudor, Delia Vlad, Carina Matei
P5 Cirrhosis Assessment in Patients Co-infected HIV-Hepatitis B Virus
Elena Dumea, Lucian Cristian Petcu, Simona Claudia Cambrea
P6 HIV late presenters in Craiova Regional Center, Romania
Florentina Dumitrescu, Augustin Cupsa, Andreea Cristina Stoian, Lucian Giubelan, Irina Niculescu, Iulian Diaconescu, Dan Hurezeanu, Livia Dragonu, Mioara Cotulbea
P7 Some aspects of malignancies in patients HIV / AIDS
Simona Manuela Erscoiu, Ionuț Cristian Popa, Denisa Stroie, Petronela Ionescu, Nedeea Duță, Camelia Dobrea, Irina Voican, Emanoil Ceaușu, Petre Iacob Calistru
P8 Factors associated with resilience among people living with HIV in Romania
Florin Lazăr
P9 Fever in HIV-infected patients: a thorny problem to be solved by the clinicians
Lucian Giubelan, Augustin Cupșa, Iulian Diaconescu, Florentina Dumitrescu, Dan Hurezeanu, Livia Dragonu, Irina Niculescu, Andreea Cristina Stoian, Oana Obretin, Mariana Stănescu, Mihai Jianu
P10 Th1, Th2, Th9, Th17 and Th22 cytokines in acute and chronic HIV-1 infection
Lana Gorenec, Snjezana Zidovec Lepej, Ivana Grgic, Ana Planinic, Janja Iscic Bes, Adriana Vince, Josip Begovac
P11 Dyslipidemia in HIV-infected patients treated with protease inhibitors – case report
Luminița Elena Horga
P12 Why use less treatment for the metabolic abnormalities in HIV patients-too many drugs?
Corina Itu, Luminița Elena Horga, Laura Augusta David-Aldea, Anca Ciorogar, Cristian Jianu, Mihaela Lupșe
P13 Sacral Herpes Zoster, with hyperalgesic form, in a patient with C3 stage HIV infection
Iuliana Caramangiu, Ovidiu Roșca, Monica Cialma, Andreea Ardeleanu, Iosif Marincu
P14 Factors associated with in-hospital mortality in tuberculous and cryptococcal meningitis
Raluca Jipa, Eliza Manea, Șerban Benea, Irina Lăpădat, Nicoleta Irimescu, Irina Panait, Cristian Niculae, Adriana Hristea
P15 Lipodystrophy: still present adverse event in resource-limited settings
Jovana Kusic, Djordje Jevtovic, Dubravka Salemovic, Jovan Ranin, Bozana Dimitrijevic, Gordana Dragovic
P16 TB and HIV coinfected patient, an emergent challenge - case report
Laura-Augusta Aldea-David
P17 Efficacy of prophylactic antiretroviral treatment in new-born infants from HIV-positive mothers in 2012-2014, for the North-Eastern part of Romania
Carmen Manciuc, Cristina Nicolau, Liviu Prisăcariu, Alexandra Largu
P18 Surveillance of mother to child transmission of HIV in Romania – 31 December 2015
Mariana Mărdărescu, Adrian Streinu-Cercel, Cristina Petre, Marieta Iancu, Sanda Vintilă, Daniela Vitelaru, Iosif Ionel, Claudiu Mihai Șchiopu, Alexandra-Henriette Mărdărescu
P19 The antiretroviral therapy failure and the need to select the effective treatment in the Republic of Moldova
Pavel Micsanschi, Tiberiu Holban, Ina Bîstrițchi, Lucia Pârțână, Angela Nagîț, Svetlana Popovici, Maria Talmaci, Irina Cucerova
P20 Disseminated cryptococcosis in a patient with C3 HIV stage and multiresistant to antiretroviral therapy with lethal evolution
Sorina Georgiana Mitrescu, Dana Mihalcea, Iulia Caramangiu, Ovidiu Roșca, Iosif Maricu
P21 Aspects of tuberculosis infection in HIV-positive patients from Romania – our experience
Anca Negru, Daniela Munteanu, Victoria Aramă, Raluca Mihăilescu, Ioan Diaconu, Remulus Catana, Cristina Popescu, Alina Orfanu, Anca Leuștean, Mihaela Rădulescu, Cătălin Tilișcan, Raluca Năstase, Violeta Molagic, Irina Duport, Cristina Dragomirescu, Ștefan Sorin Aramă
P22 Dyslipidemia in HIV-infected patients
Nicoleta M Negruț
P23 Challenges in the management of an HIV seropositive patient with psoriasis undergoing immunomodulator therapy
Violeta Elena Niță, Daniela Ioana Munteanu, Raluca Mihăilescu, Ioan Diaconu, Anca Negru, Cristina Popescu, Victoria Aramă
P24 Acute peritonitis as a sign of IRIS in an HIV-infected patient with MAC latent infection
Alina Orfanu, Cristina Popescu, Anca Leuștean, Anca Negru, Remulus Catana, Ioan Diaconu, Cătălin Tilișcan, Victoria Aramă, Sorin Ștefan Aramă
P25 The virologic outcome of the treatment of chronic hepatitis B among HIV co-infected patients on HAART
Ivana Pesic Pavlovia, Dubravka Salemovic, Jovan Ranin, Djordje Jevtovic
P26 A case of HIV encephalopathy with aphasia, agnosia, apraxia and right homonymous hemianopsia
Ovidiu Roșca, Andreea Ardeleanu, Iulia Caramangiu, Daniela Desaga, Valerica Bică, Sorina Mitrescu, Iosif Marincu
P27 Molecular footprints on human immunodeficiency virus -1 genome and association with phylogenetic clustering among subtype B infected patients in Serbia
Marina Siljic, Dubravka Salemovic, Valentina Nikolic, Djordje Jevtovic, Ivana Pesic-Pavlovic, Jovan Ranin, Marija Todorovic , Maja Stanojevic
P28 Neurosyphilis and human immunodeficiency virus infection: double challenge
Nina-Ioana Șincu, Anca Georgescu, Brândușa Țilea, Iringo Zaharia Kezdi, Andrea Incze, Cristina Gârbovan, Carmen Lucia Chiriac
P29 Differences between HIV-infected adults since childhood and non HIV-infected persons on managing everyday life
Anca Elena Luca, Florin Lazăr, Adrian Luca, Luminița Ene, Roxana Rădoi, Adina Talnariu, Silvia Suciu, Cristian Achim
P30 Molecular detection of Bartonella quintana in a HIV immunodepressed patient with fever and isolated lymphadenopathy - Case report
Diana Gabriela Iacob, Dragoș Florea, Simona Iacob
P31 Present epidemiological characteristics of HIV/AIDS newly diagnosed cases in South-Eastern Romania
Manuela Arbune, Miruna Drăgănescu, Alina Iancu
P32 The gender’s preferences among opportunists?
Ruxandra Moroti, Cristian M Niculae, Simona Merisor, Eliza Manea, Serban Benea, Andrada Stan, Raluca Hrisca, Raluca Jipa, Diana Tanase, Adriana Hristea
P33 Polymorphism of interleukin-28B gene in persons with chronic hepatitis C from Croatia
Ivana Grgic, Ana Planinic, Lana Gorenec, Snjezana Zidovec Lepej, Adriana Vince
doi:10.1186/s12879-016-1480-8
PMCID: PMC4928154  PMID: 27356504
2.  HIV transmission clusters among injecting drug users in Romania 
Romanian biotechnological letters  2017;22(1):12307-12315.
Injection drug use is increasingly an important route of HIV transmission in Romania (from 1.5% of the newly diagnosed cases prior to 2010 to 31% in 2013). In this study we investigated the viral characteristics and relationships in newly HIV infected persons who inject drugs in Bucharest, Romania.
Results
HIV-1 pol sequencing, followed by phylogenetic and clustering analysis was performed on blood from 37 injecting drug users (IDUs) newly diagnosed with HIV infection. While HIV subtype F1, the dominant strain in Romania since 1990, remains prevalent, new subtypes were found including G, B, B/G and B/F recombinants. Overall, 27 of the available sequences (72.9%) clustered with at least one other. Network and phylogenetic analysis revealed tight monophyletic clusters for both subtypes F and G, with short genetic distances between sequences, suggesting recent numerous acute to acute transmissions or single burst-type episodes. No transmitted drug-resistance mutations were identified. Greater immunosuppression was present in subjects forming the subtype G cluster, possibly indicating a faster rate of progression associated with this subtype.
Conclusions
The recent increasing numbers of IDU related HIV transmissions in Bucharest, has resulted in closely-knit transmission networks that maychange the genetic profile of the local HIV epidemic.
PMCID: PMC5713907
HIV; transmission networks; drug use; subtype G; subtype F
3.  Outcomes of iontophoretic corneal collagen crosslinking in keratoconic eyes with very thin corneas 
Medicine  2017;96(47):e8758.
Abstract
The purpose of this retrospective study was to report the results of iontophoretic corneal collagen crosslinking (I-CXL) with riboflavin and ultraviolet A irradiation in patients affected by keratoconus, each with thinnest pachymetry values of <400 μ (with epithelium) and not treatable using standard epithelium-off technique.
Fifteen eyes of 15 patients affected by progressive keratoconus and with thinnest pachymetry values <400 μ underwent I-CXL. The uncorrected (UDVA) and corrected (CDVA) distance visual acuity, maximum and minimum keratometry (K max and K min) readings, corneal thickness at the thinnest point (CTTP), endothelial cell density (ECD), and intraocular pressure (IOP) were assessed before I-CXL, at 1, 3, 6, and 12 months postoperatively.
The mean UDVA and CDVA significantly increased 12 months after I-CXL (P = .002 for both comparisons). The K max readings significantly decreased at 6 and 12 months postoperatively (P = .04 and P = .02, respectively). The mean CTTP improved at the end of the follow-up (P = .008). ECD was unchanged. No side effects or damage to the limbal region was observed during the follow-up period.
I-CXL has been proved to be effective in halting keratoconus progression in eyes with very thin corneas, with no side effects during the follow-up period. This procedure could be used in patients with more advanced keratoconus.
doi:10.1097/MD.0000000000008758
PMCID: PMC5708971
iontophoretic corneal collagen crosslinking; keratoconus; pachymetry; riboflavin; thin cornea
4.  Lettuce‐produced hepatitis C virus E1E2 heterodimer triggers immune responses in mice and antibody production after oral vaccination 
Plant Biotechnology Journal  2017;15(12):1611-1621.
Summary
The hepatitis C virus (HCV) is a major etiologic agent for severe liver diseases (e.g. cirrhosis, fibrosis and hepatocellular carcinoma). Approximately 140 million people have chronic HCV infections and about 500 000 die yearly from HCV‐related liver pathologies. To date, there is no licensed vaccine available to prevent HCV infection and production of a HCV vaccine remains a major challenge. Here, we report the successful production of the HCV E1E2 heterodimer, an important vaccine candidate, in an edible crop (lettuce, Lactuca sativa) using Agrobacterium‐mediated transient expression technology. The wild‐type dimer (E1E2) and a variant without an N‐glycosylation site in the E2 polypeptide (E1E2∆N6) were expressed, and appropriate N‐glycosylation pattern and functionality of the E1E2 dimers were demonstrated. The humoral immune response induced by the HCV proteins was investigated in mice following oral administration of lettuce antigens with or without previous intramuscular prime with the mammalian HEK293T cell‐expressed HCV dimer. Immunization by oral feeding only resulted in development of weak serum levels of anti‐HCV IgM for both antigens; however, the E1E2∆N6 proteins produced higher amounts of secretory IgA, suggesting improved immunogenic properties of the N‐glycosylation mutant. The mice group receiving the intramuscular injection followed by two oral boosts with the lettuce E1E2 dimer developed a systemic but also a mucosal immune response, as demonstrated by the presence of anti‐HCV secretory IgA in faeces extracts. In summary, our study demonstrates the feasibility of producing complex viral antigens in lettuce, using plant transient expression technology, with great potential for future low‐cost oral vaccine development.
doi:10.1111/pbi.12743
PMCID: PMC5698045  PMID: 28419665
Hepatitis C virus; E1E2 heterodimer; N‐glycosylation; lettuce; edible vaccine; transient expression; lettuce‐produced proteins; molecular farming
5.  Overcoming Monocarboxylate Transporter 8 (MCT8)-Deficiency to Promote Human Oligodendrocyte Differentiation and Myelination 
EBioMedicine  2017;25:122-135.
Cell membrane thyroid hormone (TH) transport can be facilitated by the monocarboxylate transporter 8 (MCT8), encoded by the solute carrier family 16 member 2 (SLC16A2) gene. Human mutations of the gene, SLC16A2, result in the X-linked-inherited psychomotor retardation and hypomyelination disorder, Allan-Herndon-Dudley syndrome (AHDS). We posited that abrogating MCT8-dependent TH transport limits oligodendrogenesis and myelination. We show that human oligodendrocytes (OL), derived from the NKX2.1-GFP human embryonic stem cell (hESC) reporter line, express MCT8. Moreover, treatment of these cultures with DITPA (an MCT8-independent TH analog), up-regulates OL differentiation transcription factors and myelin gene expression. DITPA promotes hESC-derived OL myelination of retinal ganglion axons in co-culture. Pharmacological and genetic blockade of MCT8 induces significant OL apoptosis, impairing myelination. DITPA treatment limits OL apoptosis mediated by SLC16A2 down-regulation primarily signaling through AKT phosphorylation, driving myelination. Our results highlight the potential role of MCT8 in TH transport for human OL development and may implicate DITPA as a promising treatment for developmentally-regulated myelination in AHDS.
Graphical Abstract
Image 2
Highlights
•NKX2.1-based sorting enhances OL derivation from hESC•MCT8 is required for the survival of OL precursor cells•DITPA promotes OL differentiation and myelination•DITPA overrides SLC16A2 (MCT8) down-regulation to potentiate myelination
Thyroid hormone is vital for oligodendrocyte differentiation and myelination. Lee and colleagues show that MCT8 is an integral thyroid hormone transporter for oligodendrocytes derived from human embryonic stem cells. Knockdown of this transporter induces apoptosis of OLs, which could be prevented by the provision of DITPA.
doi:10.1016/j.ebiom.2017.10.016
PMCID: PMC5704066  PMID: 29111262
NKX2.1; Human embryonic stem cells; Monocarboxylate transporter 8; Allan-Herndon-Dudley syndrome; Thyroid hormone; Di-iodothyropropionic acid (DITPA); Oligodendrocytes
6.  EUS needle identification comparison and evaluation (NICE) study (with videos) 
Gastrointestinal endoscopy  2016;84(3):424-433.e2.
Background and Aims
Endoscopic ultrasound (EUS)-guided fine-needle aspiration (FNA) or biopsy is widely practiced. Optimal sonographic visualization of the needle is critical for image guided interventions. There are several commercially available needles but no bench-top testing and direct comparison of these needles to reveal their inherent echogenicity. The aims are to provide bench-top data that can be used to guide clinical applications and to promote future device research and development.
Methods
Descriptive bench-top testing and comparison. Bench-top testing of 8 commonly used EUS-FNA needles (all of 22 gauge in size): SonoTip Pro Control (Medi-Globe); Expect Slimline (Boston Scientific); EchoTip, EchoTip Ultra, EchoTip ProCore High Definition, (Cook Medical); ClearView (Conmed); EZ Shot2 (Olympus); BNX (Beacon Endoscopic); and 2 new prototype needles that are coated by echogenic polymers by Medi-Globe. Blinded evaluation of standardized and unedited videos by 43 EUS endoscopists and 17 radiologists specialized in gastrointestinal ultrasound examination that is unfamiliar with EUS needle devices.
Results
There was no significant difference in the ratings and rankings of these needles between endosonographers and radiologists. Overall, one prototype needle was rated as the best, ranking 10% to 40% higher than all other needles (p<0.01). Among the commercially available needles, the EchoTip Ultra needle and the ClearView needle were top choices. The EZ Shot 2 needle was ranked statistically lower than other needles (30%–75% worse, p<0.001).
Conclusions
All FNA needles have their inherent and different echogenicity, and these differences are similarly recognized by EUS endoscopists and radiologists. Needles with polymeric coating from the entire shaft to the needle tip may offer better echogenicity.
doi:10.1016/j.gie.2016.01.068
PMCID: PMC5570521  PMID: 26873530
Endoscopic ultrasound; needle; bench-top testing; echogenicity; tip echo-definition; shaft echo-definition; high angles better echogenicity
7.  Bacterial membrane vesicles transport their DNA cargo into host cells 
Scientific Reports  2017;7:7072.
Bacterial outer membrane vesicles (OMVs) are extracellular sacs containing biologically active products, such as proteins, cell wall components and toxins. OMVs are reported to contain DNA, however, little is known about the nature of this DNA, nor whether it can be transported into host cells. Our work demonstrates that chromosomal DNA is packaged into OMVs shed by bacteria during exponential phase. Most of this DNA was present on the external surfaces of OMVs, with smaller amounts located internally. The DNA within the internal compartments of Pseudomonas aeruginosa OMVs were consistently enriched in specific regions of the bacterial chromosome, encoding proteins involved in virulence, stress response, antibiotic resistance and metabolism. Furthermore, we demonstrated that OMVs carry DNA into eukaryotic cells, and this DNA was detectable by PCR in the nuclear fraction of cells. These findings suggest a role for OMV-associated DNA in bacterial-host cell interactions and have implications for OMV-based vaccines.
doi:10.1038/s41598-017-07288-4
PMCID: PMC5539193  PMID: 28765539
8.  Citicoline – a neuroprotector with proven effects on glaucomatous disease  
Romanian Journal of Ophthalmology  2017;61(3):152-158.
Citicoline is the generic name of cytidine-5’-diphosphocholine (CDP-choline), an endogenous compound that is able to increase the levels of neurotransmitters in the central nervous system by interacting with the synthesis of cellular membranes phospholipids, especially phosphatidylcholine. Exogenous Citicoline, administered by ingestion or injection, is hydrolyzed and dephosphorylated in order to form cytidine and choline, which resynthesize CDP-choline inside brain cells. It has proven neuroprotective effects in Alzheimer disease, stroke, and Parkinson’s disease, as well as in glaucoma and amblyopia. Citicoline acts as a neuroprotector for those patients with progressive glaucomatous disease in spite of well-controlled intraocular pressure. The purpose of this review was to outline the main features of Citicoline and the evidences of its effect in glaucoma.
PMCID: PMC5710031
Citicoline; neuroprotection; glaucoma
9.  Functional results of cataract surgery in the treatment of phacomorphic glaucoma  
Romanian Journal of Ophthalmology  2017;61(3):202-206.
Purpose. Analysis of functional outcome and complications reported in patients diagnosed with phacomorphic glaucoma, in which phacoemulsification or extracapsular extraction of the lens was performed.
Methods. The retrospective study included 38 eyes diagnosed with phacomorphic glaucoma. In 25 cases, group 1, the lens was removed by phacoemulsification and in 13 cases, group 2, by extracapsular extraction. Intraocular pressure, visual acuity, and anterior chamber depth were evaluated preoperative and postoperative. The incidence of intra and postoperative complications was analyzed. The minimum follow-up period was 12 months.
Results. The mean IOP decreased from the preoperative value of 38.4 +/ - 11.3 mmHg to 13.5 +/ - 3.4 mmHg in group 1 and 11.5 +/ - 3.2 mmHg in group 2. Persistent corneal edema was observed in 32% patients from group 1 and 23% of the patients from group 2. The inflammatory reaction of anterior chamber prevailed in patients from group 2 (46.1%). ACD modified from the preoperative mean of 1.3 +/- 0.5 mm to 2.3 +/- 0.2 mm in both groups. At the end of follow-up in both groups, the average BCVA was 0.6. 18.42% of the cases required long-term topical hypotensive therapy.
Conclusions. Both phacoemulsification and extracapsular extraction were safe and effective procedures in the treatment of phacomorphic glaucoma, ensuring a rapid functional recovery and a satisfactory long-term IOP control. Although the rate of immediate postoperative complications and the final functional outcome were better in patients treated with phacoemulsification, not all the cases could be subjected to this type of surgery.
PMCID: PMC5710039
cataract surgery; phacomorphic; glaucoma
10.  Incidence of ocular hypertension after intravitreal injection of anti-VEGF agents in the treatment of neovascular AMD  
Romanian Journal of Ophthalmology  2017;61(3):207-211.
Purpose. The assessment of the incidence of ocular hypertension over a period of 1 year in patients treated with multiple intravitreal injections of anti-VEGF agents for neovascular AMD.
Methods. The study comprised 58 eyes diagnosed with neovascular age-related macular degeneration and receiving PRN intravitreal treatment with anti-VEGF agents (bevacizumab or aflibercept). The follow-up period was 1 year. Intraocular pressure was measured by using the Goldmann applanation tonometry before the intravitreal injection, at 24 hours after the administration of the anti-VEGF agent and at 1 and 4 weeks. Patients diagnosed with glaucoma or who underwent ophthalmic surgery were excluded.
Results. The patients received an average of 7.54 intravitreal injections. The mean baseline intraocular pressure was 15.3 mm Hg; 19.8 mm Hg at 24 hours; 17,4 mmHg at 1 week and 14.8 mmHg at 4 weeks after the administration of the anti-VEGF agent. 4 patients required long-term topical hypotensive treatment. Raised intraocular pressure was related to increased frequency of treatment. At 1 year follow up, an average difference of 2.1 mmHg compared to baseline was registered in the cases that have received more than 6 intravitreal injections. By comparison, in the cases treated with a reduced number of doses of intravitreal anti VEGF agent, the difference from baseline was 0,9 mmHg. There were no significant differences in mean IOP depending on the anti VEGF (bevacizumab or aflibercept) agent used.
Conclusions. Intravitreal treatment with anti VEGF agents produces a transient increase in intraocular pressure, predominantly immediately following administration, without causing long-term increased values.
PMCID: PMC5710040
intraocular pressure; intravitreal injection; neovascular AMD; anti-VEGF
11.  Trends in Regenerative Medicine: Repigmentation in Vitiligo Through Melanocyte Stem Cell Mobilization 
Medicinal research reviews  2016;37(4):907-935.
Vitiligo is the most frequent human pigmentary disorder, characterized by progressive autoimmune destruction of mature epidermal melanocytes. Of the current treatments offering partial and temporary relief, ultraviolet (UV) light is the most effective, coordinating an intricate network of keratinocyte and melanocyte factors that control numerous cellular and molecular signaling pathways. This UV-activated process is a classic example of regenerative medicine, inducing functional melanocyte stem cell populations in the hair follicle to divide, migrate, and differentiate into mature melanocytes that regenerate the epidermis through a complex process involving melanocytes and other cell lineages in the skin. Using an in-depth correlative analysis of multiple experimental and clinical data sets, we generated a modern molecular research platform that can be used as a working model for further research of vitiligo repigmentation. Our analysis emphasizes the active participation of defined molecular pathways that regulate the balance between stemness and differentiation states of melanocytes and keratinocytes: p53 and its downstream effectors controlling melanogenesis; Wnt/β-catenin with proliferative, migratory, and differentiation roles in different pigmentation systems; integrins, cadherins, tetraspanins, and metalloproteinases, with promigratory effects on melanocytes; TGF-β and its effector PAX3, which control differentiation. Our long-term goal is to design pharmacological compounds that can specifically activate melanocyte precursors in the hair follicle in order to obtain faster, better, and durable repigmentation.
doi:10.1002/med.21426
PMCID: PMC5466503  PMID: 28029168
vitiligo; repigmentation; melanocyte stem cell; melanoblast; regeneration
12.  Ultrasound and microbubble induced release from intracellular compartments 
BMC Biotechnology  2017;17:45.
Background
Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB-induced release of cellular materials to the extracellular milieu. This study investigates USMB effects on the molecular release from cells through membrane-disruption and exocytosis.
Results
USMB induced the release of 19% and 67% of GFP from the cytoplasm in viable and non-viable cells, respectively. Tfn release from early/recycling endosomes increased by 23% in viable cells upon USMB treatment. In addition, the MFI of LAMP-1 antibody increased by 50% in viable cells, suggesting USMB-stimulated lysosome exocytosis. In non-viable cells, labeling of LAMP-1 intracellular structures in the absence of cell permeabilization by detergents suggests that USMB-induced cell death correlates with lysosomal permeabilization.
Conclusions
In conclusion, USMB enhanced the molecular release from the cytoplasm, lysosomes, and early/recycling endosomes.
Electronic supplementary material
The online version of this article (doi:10.1186/s12896-017-0364-3) contains supplementary material, which is available to authorized users.
doi:10.1186/s12896-017-0364-3
PMCID: PMC5437622  PMID: 28521780
Ultrasound and microbubble; Sonoporation; Endocytosis; Exocytosis; Intracellular uptake; Intracellular release; Acoustic cavitation bioeffects; In vitro ultrasound bioeffects; Cellular bioeffects of ultrasound
13.  Pseudophakic bullous keratopathy  
Pseudophakic bullous keratopathy is characterized by corneal stromal edema with epithelial and subepithelial bullae due to cell loss and endothelial decompensation through trauma during cataract surgery. Patients present decreased vision, tearing, and pain caused by ruptured epithelial bullae. Cataract affects approximately 20 million people worldwide, and this complication can occur in 1 to 2% of the cataract surgeries. This study reviewed the bullous keratopathy etiopathogenesis and the clinical and surgical treatment available for this corneal disease.
PMCID: PMC5710027
corneal disease treatment; corneal surgery; bullous keratopathy; cataract extraction
14.  The acyltransferase LYCAT controls specific phosphoinositides and related membrane traffic 
Molecular Biology of the Cell  2017;28(1):161-172.
Phosphoinositides (PIPs) control membrane traffic. PIPs have an acyl profile unique among phospholipids. The acyltransferase LYCAT localizes to phosphatidylinositol synthase vesicles, selectively regulates levels and locale of PIPs, and controls related membrane traffic, indicating that dynamic acyl remodeling selectively controls certain PIPs.
Phosphoinositides (PIPs) are key regulators of membrane traffic and signaling. The interconversion of PIPs by lipid kinases and phosphatases regulates their functionality. Phosphatidylinositol (PI) and PIPs have a unique enrichment of 1-stearoyl-2-arachidonyl acyl species; however, the regulation and function of this specific acyl profile remains poorly understood. We examined the role of the PI acyltransferase LYCAT in control of PIPs and PIP-dependent membrane traffic. LYCAT silencing selectively perturbed the levels and localization of phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] and phosphatidylinositol-3-phosphate and the membrane traffic dependent on these specific PIPs but was without effect on phosphatidylinositol-4-phosphate or biosynthetic membrane traffic. The acyl profile of PI(4,5)P2 was selectively altered in LYCAT-deficient cells, whereas LYCAT localized with phosphatidylinositol synthase. We propose that LYCAT remodels the acyl chains of PI, which is then channeled into PI(4,5)P2. Our observations suggest that the PIP acyl chain profile may exert broad control of cell physiology.
doi:10.1091/mbc.E16-09-0668
PMCID: PMC5221620  PMID: 28035047
15.  B2 adrenergic receptors and morphological changes of the enteric nervous system in colorectal adenocarcinoma 
World Journal of Gastroenterology  2017;23(7):1250-1261.
AIM
To study the morphology of the enteric nervous system and the expression of beta-2 adrenergic (B2A) receptors in primary colorectal cancer.
METHODS
In this study, we included forty-eight patients with primary colorectal cancer and nine patients for control tissue from the excision of a colonic segment for benign conditions. We determined the clinicopathological features and evaluated the immunohistochemical expression pattern of B2A receptors as well as the morphological changes of the enteric nervous system (ENS). In order to assess statistical differences, we used the student t-test for comparing the means of two groups and one-way analysis of variance with Bonferroni’s post hoc analysis for comparing the means of more than two groups. Correlations were assessed using the Pearson’s correlation coefficient.
RESULTS
B2A receptors were significantly associated with tumor grading, tumor size, tumor invasion, lymph node metastasis (P < 0.05), while there were no statistically significant associations with gender, CRC location and gross appearance (P > 0.05). We observed, on one hand, a decrease of the relative area for both Auerbach and Meissner plexuses with the increase of the tumor grading, and on the other hand, an increase of the relative area of other nervous elements not in the Meissner plexus or in the Auerbach plexus with the tumor grading. For G1 tumors we found that epithelial B2A area showed an inverse correlation with the Auerbach plexus areas [r(14) = -0.531, P < 0.05], while for G2 tumors, epithelial B2A areas showed an indirect variation with both the Auerbach plexus areas [r(14) = -0.453, P < 0.05] and the Meissner areas [r(14) = -0.825, P < 0.01]. For G3 tumors, the inverse dependence increased for both Auerbach [r(14) = -0.587, P < 0.05] and Meissner [r(14) = -0.934, P < 0.05] plexuses.
CONCLUSION
B2A receptors play an important role in colorectal carcinogenesis and can be utilized as prognostic factors. Furthermore, study of the ENS in colorectal cancer may lead to targeted molecular therapies.
doi:10.3748/wjg.v23.i7.1250
PMCID: PMC5323450
Beta-2 adrenergic receptors; Enteric nervous system; Colorectal adenocarcinoma; Spectral unmixing immunohistochemistry; Tumor grading
16.  Evaluation of Iontophoretic Collagen Cross-linking for Early Stage of Progressive Keratoconus Compared to Standard Cross-linking: A Non-Inferiority Study 
Ophthalmology and Therapy  2017;6(1):147-160.
Introduction
The purpose of this study was to evaluate the efficacy and safety of iontophoretic collagen cross-linking (I-CXL) compared to epithelium-off standard collagen cross-linking (CXL) in treating the early stages of progressive keratoconus.
Methods
This retrospective cohort study at Oftaprof Clinic, Iasi, Romania included 40 eyes of 40 patients with progressive keratoconus stage I according to the Amsler classification who underwent I-CXL and the results were compared with a matched group of 40 eyes from 40 patients who received standard CXL. The follow-up period was 24 months. Uncorrected (UCVA) and corrected (CDVA) distance visual acuities, corneal topography, and pachymetry were evaluated in all patients.
Results
The mean patient age was 26.52 ± 3.77 years for the standard CXL group and 28.32 ± 4.91 for the I-CXL group. The mean UCVA and CDVA improved significantly in both groups. At 12 months, the improvement of UCVA was greater in the I-CXL group (P < 0.05). There was a statistically significant different trend in CDVA between groups with a more favorable outcome for the standard CXL group (P < 0.01). The manifest cylinder decreased by a mean of 0.962 ± 0.114 D in the epithelium-off CXL group and by 0.831 ± 0.082 D in the I-CXL group (P < 0.001). At 24 months, the K max values improved by 1.2 ± 0.199 D in the standard group and by 0.908 ± 0.177 D in the I-CXL group (P < 0.001).
Conclusion
All parameters either improved or remained unchanged after the iontophoretic collagen cross-linking intervention. I-CXL was found to be as effective as the standard technique.
Electronic supplementary material
The online version of this article (doi:10.1007/s40123-017-0076-8) contains supplementary material, which is available to authorized users.
doi:10.1007/s40123-017-0076-8
PMCID: PMC5449295  PMID: 28160249
Keratoconus; Iontophoretic collagen cross-linking; Standard cross-linking; Riboflavin
17.  Terson’s Syndrome – case report  
Terson’s Syndrome is represented by a vitreous, retrohyaloid, retinal, or subretinal hemorrhage occurring consequent to an acute intracranial hemorrhage or elevated intracranial pressure. The outcome may include a complete clearing of the blood and the restoration of VA or persistent hemorrhage.
This report presents the case of a 43-year-old woman who underwent bilateral surgery for a persistent vitreous hemorrhage and a hematoma underneath the internal limiting membrane in the left eye. The event followed shortly after a subarachnoid hemorrhage due to the rupture of a posterior communicating artery aneurism. Vitrectomy was performed in both eyes, together with the peeling of the internal limiting membrane in the left eye, followed by a bilateral good outcome.
PMCID: PMC5710052
Terson’s syndrome; persistent hemorrhage; epiretinal membrane; vitrectomy
18.  Variation in branchial expression among insulin-like growth-factor binding proteins (igfbps) during Atlantic salmon smoltification and seawater exposure 
BMC Physiology  2017;17:2.
Background
In preparation for migration from freshwater to marine habitats, Atlantic salmon (Salmo salar L.) undergo smoltification, a transformation that includes the acquisition of hyposmoregulatory capacity. The growth hormone (Gh)/insulin-like growth-factor (Igf) axis promotes the development of branchial ionoregulatory functions that underlie ion secretion. Igfs interact with a suite of Igf binding proteins (Igfbps) that modulate hormone activity. In Atlantic salmon smolts, igfbp4,−5a,−5b1,−5b2,−6b1 and−6b2 transcripts are highly expressed in gill. We measured mRNA levels of branchial and hepatic igfbps during smoltification (March, April, and May), desmoltification (July) and following seawater (SW) exposure in March and May. We also characterized parallel changes in a broad suite of osmoregulatory (branchial Na+/K+-ATPase (Nka) activity, Na + /K + /2Cl − cotransporter 1 (nkcc1) and cystic fibrosis transmembrane regulator 1 (cftr1) transcription) and endocrine (plasma Gh and Igf1) parameters.
Results
Indicative of smoltification, we observed increased branchial Nka activity, nkcc1 and cftr1 transcription in May. Branchial igfbp6b1 and -6b2 expression increased coincidentally with smoltification. Following a SW challenge in March, igfbp6b1 showed increased expression while igfbp6b2 exhibited diminished expression. igfbp5a,−5b1 and−5b2 mRNA levels did not change during smolting, but each had lower levels following a SW exposure in March.
Conclusions
Salmonids express an especially large suite of igfbps. Our data suggest that dynamic expression of particular igfbps accompanies smoltification and SW challenges; thus, transcriptional control of igfbps may provide a mechanism for the local modulation of Igf activity in salmon gill.
doi:10.1186/s12899-017-0028-5
PMCID: PMC5242021  PMID: 28100217
Insulin-like growth-factor; Binding proteins; Growth hormone; Atlantic salmon; Smoltification; Osmoregulation; Gill; Liver; Salinity
19.  Integrins and Cell Metabolism: An Intimate Relationship Impacting Cancer 
Integrins are important regulators of cell survival, proliferation, adhesion and migration. Once activated, integrins establish a regulated link between the extracellular matrix and the cytoskeleton. Integrins have well-established functions in cancer, such as in controlling cell survival by engagement of many specific intracellular signaling pathways and in facilitating metastasis. Integrins and associated proteins are regulated by control of transcription, membrane traffic, and degradation, as well as by a number of post-translational modifications including glycosylation, allowing integrin function to be modulated to conform to various cellular needs and environmental conditions. In this review, we examine the control of integrin function by cell metabolism, and the impact of this regulation in cancer. Within this context, nutrient sufficiency or deprivation is sensed by a number of metabolic signaling pathways such as AMP-activated protein kinase (AMPK), mammalian target of rapamycin (mTOR) and hypoxia-inducible factor (HIF) 1, which collectively control integrin function by a number of mechanisms. Moreover, metabolic flux through specific pathways also controls integrins, such as by control of integrin glycosylation, thus impacting integrin-dependent cell adhesion and migration. Integrins also control various metabolic signals and pathways, establishing the reciprocity of this regulation. As cancer cells exhibit substantial changes in metabolism, such as a shift to aerobic glycolysis, enhanced glucose utilization and a heightened dependence on specific amino acids, the reciprocal regulation of integrins and metabolism may provide important clues for more effective treatment of various cancers.
doi:10.3390/ijms18010189
PMCID: PMC5297821  PMID: 28106780
AMPK; mTOR; HIF1; membrane traffic; glycosylation; hypoxia; nutrient deficit; cancer metabolism; metabolic stress
20.  Nanostructured SnO2–ZnO composite gas sensors for selective detection of carbon monoxide 
A series of SnO2–ZnO composite nanostructured (thin) films with different amounts of SnO2 (from 0 to 50 wt %) was prepared and deposited on a miniaturized porous alumina transducer using the sol–gel and dip coating method. The transducer, developed by our research group, contains Au interdigital electrodes on one side and a Pt heater on the other side. The sensing films were characterized using SEM and AFM techniques. Highly toxic and flammable gases (CO, CO2, CH4, and C3H8) were tested under lab conditions (carrier gas was dry air) using a special gas sensing cell developed by our research group. The gas concentrations varied between 5 and 2000 ppm and the optimum working temperatures were in the range of 210–300 °C. It was found that the sensing performance was influenced by the amount of oxide components present in the composite material. Improved sensing performance was achieved for the ZnO (98 wt %)–SnO2 (2 wt %) composite as compared to the sensors containing only the pristine oxides. The sensor response, cross-response and recovery characteristics of the analyzed materials are reported. The high sensitivity (R S = 1.21) to low amounts of CO (5 ppm) was reported for the sensor containing a composite sensitive film with ZnO (98 wt %)–SnO2 (2 wt %). This sensor response to CO was five times higher as compared to its response to CO2, CH4, and C3H8, thus the sensor is considered to be selective for CO under these test conditions.
doi:10.3762/bjnano.7.195
PMCID: PMC5238679  PMID: 28144552
composite thin film sensor; dip coating; low detection limit; miniaturized alumina transducer; selective CO detection; sol–gel
21.  Myocardial Gene Expression Profiling to Predict and Identify Cardiac Allograft Acute Cellular Rejection: The GET-Study 
PLoS ONE  2016;11(11):e0167213.
Aims
Serial invasive endomyocardial biopsies (EMB) remain the gold standard for acute cellular rejection (ACR) diagnosis. However histological grading has several limitations. We aimed to explore the value of myocardial Gene Expression Profiling (GEP) for diagnosing and identifying predictive biomarkers of ACR.
Methods
A case-control study nested within a retrospective heart transplant patients cohort included 126 patients with median (IQR) age 50 (41–57) years and 111 (88%) males. Among 1157 EMB performed, 467 were eligible (i.e, corresponding to either ISHLT grade 0 or ≥3A), among which 36 were selected for GEP according to the grading: 0 (CISHLT, n = 13); rejection ≥3A (RISHLT, n = 13); 0 one month before ACR (BRISHLT, n = 10).
Results
We found 294 genes differentially expressed between CISHLT and RISHLT, mainly involved in immune activation, and inflammation. Hierarchical clustering showed a clear segregation of CISHLT and RISHLT groups and heterogeneity of GEP within RISHLT. All EMB presented immune activation, but some RISHLT EMB were strongly subject to inflammation, whereas others, closer to CISHLT, were characterized by structural modifications with lower inflammation level. We identified 15 probes significantly different between BRISHLT and CISHLT, including the gene of the muscular protein TTN. This result suggests that structural alterations precede inflammation in ACR. Linear Discriminant Analysis based on these 15 probes was able to identify the histological status of every 36 samples.
Conclusion
Myocardial GEP is a helpful method to accurately diagnose ACR, and predicts rejection one month before its histological occurrence. These results should be considered in cardiac allograft recipients’ care.
doi:10.1371/journal.pone.0167213
PMCID: PMC5127573  PMID: 27898719
22.  The 12th Edition of the Scientific Days of the National Institute for Infectious Diseases “Prof. Dr. Matei Bals” and the 12th National Infectious Diseases Conference 
Niculae, Cristian-Mihail | Manea, Eliza | Jipa, Raluca | Merisor, Simona | Moroti, Ruxandra | Benea, Serban | Hristea, Adriana | Neguț, Alina Cristina | Săndulescu, Oana | Streinu-Cercel, Anca | Mărculescu, Dana | Andrei, Magdalena Lorena | Ilie, Veronica | Popa, Marcela | Bleotu, Coralia | Chifiriuc, Carmen | Popa, Mircea Ioan | Streinu-Cercel, Adrian | Orfanu, Alina | Popescu, Cristina | Leuștean, Anca | Catană, Remulus | Negru, Anca | Badea, Alexandra | Orfanu, Radu | Tilișcan, Cătălin | Aramă, Victoria | Aramă, Ştefan Sorin | Vișan, Constanța-Angelica | Drăgănescu, Anca-Cristina | Bilașco, Anuța | Kouris, Camelia | Merișescu, Mădălina | Vasile, Magdalena | Slavu, Diana-Maria | Vintilă, Sabina | Osman, Endis | Oprea, Alina | Sandu, Sabina | Luminos, Monica | Orfanu, Alina | Aramă, Victoria | Aramă, Ştefan Sorin | Leuştean, Anca | Catană, Remulus | Negru, Anca | Popescu, Gabriel Adrian | Popescu, Cristina | Stanculete, Ramona Georgiana | Enoiu, Ana Vaduva | Marinescu, Adelina Raluca | Lazureanu, Voichita | Marinescu, Adelina-Raluca | Crișan, Alexandru | Lăzureanu, Voichița | Musta, Virgil | Nicolescu, Narcisa | Laza, Ruxandra | Negru, Anca-Ruxandra | Munteanu, Daniela-Ioana | Mihăilescu, Raluca | Catană, Remulus | Dorobăț, Olga | Rafila, Alexandru | Căpraru, Emilia | Niculescu, Marius | Marinescu, Rodica | Lupescu, Olivera | Predescu, Vlad | Streinu-Cercel, Adrian | Aramă, Victoria | Tălăpan, Daniela | Popescu, Ramona Ștefania | Bradu, Luminița | Florea, Dragoș | Streinu-Cercel, Adrian | Leca, Daniela Anicuta | Bunea, Elena | Teodor, Andra | Miftode, Egidia | Merișescu, Mădălina | Jugulete, Gheorghiță | Streinu-Cercel, Adrian | Florea, Dragoș | Luminos, Monica | Popescu, Ramona Ștefania | Dobrotă, Anamaria | Ilie, Adina | Preoțescu, Liliana Lucia | Hristea, Adriana | Jipa, Raluca | Irimescu, Nicoleta | Panait, Irina | Manea, Eliza | Merisor, Simona | Niculae, Cristian | Tălăpan, Daniela | Gavriliu, Liana Cătălina | Benea, Otilia Elisabeta | Benea, Șerban | Rafila, Alexandru | Dorobăț, Olga | Popoiu, Mona | Dragonu, Livia | Cupşa, Augustin | Diaconescu, Iulian | Niculescu, Irina | Giubelan, Lucian | Dumitrescu, Florentina | Stoian, Andreea Cristina | Guţă, Camelia | Puiu, Simona | Irina, Bunescu | Vallée, Marilyse | Huletsky, Ann | Boudreau, Dominique K. | Bérubé, Ève | Giroux, Richard | Longtin, Jean | Longtin, Yves | Bergeron, Michel G. | Roșculeț, Cleo Nicoleta | Toma, Dalila-Ana | Ciuca, Catrinel | Tălăpan, Daniela | Apostolescu, Cătălin | Rogoz, Andrei | Stangaciu, Andrei | Mitescu, Viorica | Vladoiu, Tudor | Iovănescu, Doina | Oana, Michaela | Costin, Simona | Neguț, Alina Cristina | Săndulescu, Oana | Streinu-Cercel, Anca | Moțoi, Maria Magdalena | Popa, Mircea Ioan | Streinu-Cercel, Adrian | Tălăpan, Daniela | Dorobăț, Olga Mihaela | Popoiu, Mona | Mihai, Alexandru | Iovănescu, Doina | Roşculeț, Cleo | Apostolescu, Cătălin | Popescu, Gabriel-Adrian | Abagiu, Adrian | Moroti-Constantinescu, Ruxandra | Hristea, Adriana | Aramă, Victoria | Benea, Otilia | Simoiu, Mădălina | Bacruban, Rodica | Streinu-Cercel, Adrian | Rafila, Alexandru | Dorobăț, Olga Mihaela | Tălăpan, Daniela | Mihai, Alexandru | Bădicuț, Ioana | Popoiu, Mona | Borcan, Alina | Rafila, Alexandru | Popescu, Gabriel Adrian | Hurmuzache, Mihnea | Enache, Georgiana | Ciocan, Alexandra | Bararu, Mircea | Popazu, Madalina | Iovănescu, Doina Viorica | Roșculeț, Cleo Nicoleta | Rogoz, Andrei | Apostolescu, Cătălin Gabriel | Mitescu, Viorica | Vladoiu, Tudor | Toma, Dalila | Ciuca, Catrinel | Iliescu, Laura | Minzala, Georgiana | Toma, Letitia | Baciu, Mihaela | Tanase, Alina | Orban, Carmen | Pantea, Victor | Placinta, Gheorghe | Cebotarescu, Valentin | Cojuhari, Lilia | Jimbei, Paulina | Popescu, Cristina | Leuștean, Anca | Dragomirescu, Cristina | Orfanu, Alina | Murariu, Cristina | Stratan, Laurențiu | Badea, Alexandra | Tilișcan, Cătălin | Munteanu, Daniela | Năstase, Raluca | Molagic, Violeta | Rădulescu, Mihaela | Catană, Remulus | Aramă, Victoria | Popescu, Cristina | Stratan, Laurențiu | Catană, Remulus | Leuștean, Anca | Dragomirescu, Cristina | Badea, Alexandra | Murariu, Cristina | Năstase, Raluca | Molagic, Violeta | Munteanu, Daniela | Tilișcan, Cătălin | Rădulescu, Mihaela | Orfanu, Alina | Diaconu, Ioan | Negru, Anca | Bodosca, Iulia | Niță, Violeta | Aramă, Victoria | Leuștean, Anca | Aramă, Victoria | Orfanu, Alina | Catană, Remulus | Stratan, Laurențiu | Dragomirescu, Cristina | Murariu, Cristina | Badea, Alexandra | Tilișcan, Cătălin | Munteanu, Daniela | Molagic, Violeta | Năstase, Raluca | Rădulescu, Mihaela | Popescu, Cristina | Popescu, Cristina | Dragomirescu, Cristina | Leuștean, Anca | Murariu, Cristina | Stratan, Laurențiu | Badea, Alexandra | Catană, Remulus | Orfanu, Alina | Năstase, Raluca Mihaela | Molagic, Violeta | Munteanu, Daniela | Tilișcan, Cătălin | Aramă, Victoria | Aramă, Victoria | Catană, Remulus | Dragomirescu, Cristina | Murariu, Cristina | Leuștean, Anca | Stratan, Laurențiu | Badea, Alexandra | Orfanu, Alina | Negru, Anca | Năstase, Raluca | Molagic, Violeta | Munteanu, Daniela | Tilișcan, Cătălin | Rădulescu, Mihaela | Diaconu, Ioan | Niță, Violeta | Bodoșca, Iulia | Popescu, Cristina | Popescu, Cristina | Badea, Alexandra | Leuștean, Anca | Orfanu, Alina | Negru, Anca | Stratan, Laurențiu | Dragomirescu, Cristina | Catană, Remulus | Murariu, Cristina | Molagic, Violeta | Năstase, Raluca | Tilișcan, Cătălin | Munteanu, Daniela | Rădulescu, Mihaela | Diaconu, Ioan | Niță, Violeta | Bodoșca, Iulia | Aramă, Victoria | Popescu, Cristina | Orfanu, Alina | Leuștean, Anca | Badea, Alexandra | Stratan, Laurențiu | Catană, Remulus | Tilișcan, Cătălin | Aramă, Victoria | Popescu, Cristina | Murariu, Cristina | Dragomirescu, Cristina | Leuștean, Anca | Stratan, Laurențiu | Orfanu, Alina | Badea, Alexandra | Catană, Remulus | Negru, Anca | Tilișcan, Cătălin | Munteanu, Daniela | Rădulescu, Mihaela | Molagic, Violeta | Năstase, Raluca Mihaela | Diaconu, Ioan Alexandru | Bodoșca, Iulia | Niță, Violeta | Aramă, Victoria | Erturk, Yagmur | Săndulescu, Oana | Neguț, Alina Cristina | Șchiopu, Claudiu Mihai | Streinu-Cercel, Adrian | Streinu-Cercel, Anca | Molagic, Violeta | Tilișcan, Cătălin | Popescu, Cristina | Mihăilescu, Raluca | Munteanu, Daniela | Năstase, Raluca | Negru, Anca | Tenita, Angelica | Aramă, Victoria | Aramă, Ștefan Sorin | Iacob, Simona Alexandra | Iacob, Diana Gabriela | Luminos, Monica | Streinu-Cercel, Anca | Săndulescu, Oana | Predescu, Mioara | Mărdărescu, Alexandra | Tilișcan, Cătălin | Săndulescu, Mihai | Șchiopu, Claudiu Mihai | Streinu-Cercel, Adrian | Roșculeț, Cleo Nicoleta | Ciuca, Catrinel Olimpia | Toma, Dalila Ana | Apostolescu, Cătălin Gabriel | Rogoz, Andrei | Mitu, Cristina Elena | Stangaciu, Andrei | Mitescu, Viorica Daniela | Vladoiu, Tudor Gheorghe | Iovănescu, Doina Viorica | Săndulescu, Oana | Streinu-Cercel, Anca | Stoica, Monica Andreea | Preoțescu, Liliana Lucia | Manolache, Daniela | Ceapraga, Gabriela Jana | Moțoi, Maria Magdalena | Bradu, Luminița | Ilie, Adina | Mircea, Gabriela | Durbală, Ionel | Streinu-Cercel, Adrian | Russu, Irina | Holban, Tiberiu | Pantilimonov, Tatiana | Chiriacov, Galina | Macvovei, Arcadie | Scorohodico, Elena | Dmitriev, Oleg | Costache, Diana Alexandra | Benea, Anca | Manea, Eliza | Niculae, Cristian | Jipa, Raluca | Hristea, Adriana | Benea, Elisabeta | Moroti, Ruxandra | Benea, Șerban | Mitran, Mihai | Georgescu, Carmen | Mitran, Loredana | Vladareanu, Simona | Magirescu, Andreea Ioana | Andreev, Viorica | Nicolau, Cristina | Largu, Alexandra | Dorobat, Carmen | Manciuc, Carmen | Andreev, Viorica | Magirescu, Andreea Ioana | Isac, Ina | Nicolau, Cristina | Largu, Alexandra | Dorobat, Carmen | Manciuc, Carmen | Șerban, Iulia Gabriela | Resul, Ghiulendan | Marcaș, Consuela | Marincu, Iosif | Poptelecan, Patricia | Trincă, Bogdan | Mitrescu, Sorina | Tudor, Anca | Vlad, Daliborca | Tirnea, Livius | Baydaroglu, Nurcan | Neguț, Alina Cristina | Săndulescu, Oana | Manolache, Daniela | Ceapraga, Gabriela | Stoica, Monica Andreea | Streinu-Cercel, Anca | Streinu-Cercel, Adrian | Manciuc, Carmen | Pagute, Mariana | Nicolau, Cristina | Dorobăț, Carmen | Largu, Alexandra | Diaconu, Ioan-Alexandru | Stratan, Laurențiu | Ion, Daniela | Nichita, Luciana | Popescu, Cristina | Năstase, Raluca | Munteanu, Daniela | Molagic, Violeta | Tilișcan, Cătălin | Rădulescu, Mihaela | Diaconu, Alexandra | Negru, Anca | Orfanu, Alina | Dragomirescu, Cristina | Catană, Remulus | Leuștean, Anca | Duport-Dodot, Irina | Murariu, Cristina | Bodoșca, Iulia | Niță, Violeta | Badea, Alexandra | Aramă, Victoria | Mărdărescu, Mariana | Petre, Cristina | Iancu, Marieta | Ungurianu, Rodica | Cibea, Alina | Drăghicenoiu, Ruxandra | Tudor, Ana Maria | Vlad, Delia | Petrea, Sorin | Matei, Carina | Oțelea, Dan | Crăciun, Carmen | Anghelina, Cristian | Mărdărescu, Alexandra | Dumea, Elena | Streinu-Cercel, Adrian | Rugină, Sorin | Petcu, Lucian Cristian | Halichidis, Stela | Cambrea, Simona Claudia | Chiriac, Carmen | Bodnar, Nina-Ioana | Zaharia-Kezdi, Iringo-Erzsebet | Gîrbovan, Cristina | Incze, Andrea | Georgescu, Anca Meda | Iacob, Simona Alexandra | Iacob, Diana Gabriela | Panaitescu, Eugenia | Luminos, Monica | Cojocaru, Manole | Iacob, Simona Alexandra | Iacob, Diana Gabriela | Luminos, Monica | Laurențiu, Vochita | Andreia, Vochita | Radu, Opreanu | Bogdan, Trinca | Ovidiu, Rosca | Iosif, Marincu | Zamfir, Ramona | Angelescu, Alina | Popa, Alena Andreea | Jipa, Raluca | Moroti, Ruxandra | Hristea, Adriana | Gavriliu, Liana | Benea, Șerban | Benea, Elisabeta | Popa, Alena-Andreea | Ducu, Georgeta | Camburu, Daniela | Cozma, Alina | Podani, Manuela | Dumitriu, Roxana | Gavriliu, Liana | Benea, Șerban | Benea, Elisabeta | Stoian, Andreea Cristina | Dumitrescu, Florentina | Cupșa, Augustin | Giubelan, Lucian | Niculescu, Irina | Ionescu, Loredana | Dragonu, Livia | Abagiu, Adrian Octavian | Stoica, Loredana Nicoleta | Blaga, Catrinel | Koulosousas, Archontis | Ștefănescu, Roxana | Atomoaie, Alice | Paraschiv, Florentina | Duna, Florin Matache | Olteanu, Rodica | Ion, Roxana | Zota, Alexandra | Jaballah, Isra Ennour | Mahfoud, Lara | Preda, Georgeta | Constantin, Magda | Nicolae, Ilinca | Ene, Corina Daniela | Mitran, Mădălina Irina | Benea, Vasile | Tampa, Mircea | Georgescu, Simona Roxana | Bodoșca, Iulia Cristina | Murariu, Cristina | Tilișcan, Cătălin | Aramă, Victoria | Popescu, Cristina | Munteanu, Daniela | Rădulescu, Mihaela | Molagic, Violeta | Năstase, Raluca | Orfanu, Alina | Leuștean, Anca | Catană, Remulus | Negru, Anca | Streinu-Cercel, Adrian | Aramă, Sorin | Caramăngiu, Iuliana | Rosca, Ovidiu | Cialma, Monica | Opreanu, Radu | Vochita, Laurențiu | Marincu, Iosif | Murărescu, Vlad | Palaghiță, Marilena | Neguț, Alina Cristina | Camburu, Cornel | Streinu-Cercel, Adrian | Duşan, Irina | Poptelecan, Patricia | Trincă, Bogdan | Mitrescu, Sorina | Tirnea, Livius | Marincu, Iosif | Nicolescu, Narcisa | Crișan, Alexandru | Lăzureanu, Voichița | Laza, Ruxandra | Musta, Virgil | Marinescu, Adelina-Raluca | Bîrlad, Andreea | Miron, Victor Daniel | Drăgănescu, Anca Cristina | Vișan, Constanța-Angelica | Bilașco, Anuța | Pițigoi, Daniela | Săndulescu, Oana | Luminos, Monica Luminița | Luminos, Monica | Osman, Endis | Vasile, Magdalena | Drăgănescu, Anca Cristina | Vișan, Constanța-Angelica | Bilașco, Anuța | Kouris, Camelia | Șchiopu, Sabina | Merișescu, Mădălina | Luminos, Monica | Drăgănescu, Anca Cristina | Vișan, Constanța-Angelica | Bilașco, Anuța | Kouris, Camelia | Osman, Endis | Vintilă, Sabina | Vasile, Magda | Merișescu, Mădălina | Gavriliu, Liana Cătălina | Benea, Otilia Elisabeta | Angelescu, Alina | Zamfir, Ramona | Camburu, Daniela | Ducu, Georgeta | Cozma, Alina | Dumitriu, Roxana | Podani, Manuela | Benea, Șerban | Ionică, Mihaela | Jugulete, Gheorghiță | Stăncescu, Adina | Popescu, Cristina Elena | Marin, Luminița | Zaharia, Diana | Dumitrescu, Cristina | Tudor, Lucia | Vintilă, Sabina | Vișan, Constanța-Angelica | Drăgănescu, Anca Cristina | Bilașco, Anuța | Vasile, Magda | Merișescu, Mădălina | Kouris, Camelia | Negulescu, Cristina | Osman, Endis | Slavu, Diana-Maria | Vintilă, Sabina | Pițigoi, Daniela | Luminos, Monica | Caliman-Sturdza, Olga Adriana | Roșculeț, Cleo | Ciuca, Catrinel Olimpia | Toma, Dalila | Apostolescu, Cătălin | Rogoz, Andrei | Stangaciu, Andrei | Mitescu, Viorica | Iovănescu, Doina | Camburu, Cornel | Manu, Bogdana | Vaduva-Enoiu, Ana | Stanculete, Ramona Georgiana | Marinescu, Adelina Raluca | Lazureanu, Voichita Elena | Niță, Elena-Violeta | Dumitru, Sînziana | Munteanu, Daniela-Ioana | Negru, Anca Ruxandra | Catană, Remulus | Diaconu, Ioan | Manu, Bogdana | Ionescu, Ligia | Ion, Liliana | Tilișcan, Cătălin | Aramă, Victoria | Iovănescu, Doina Viorica | Roșculeț, Cleo Nicoleta | Rogoz, Andrei | Apostolescu, Cătălin | Mitescu, Viorica | Vladoiu, Tudor | Toma, Dalila | Ciuca, Catrinel | Șerban, Iulia Gabriela | Neacșu, Marioara | Georgescu, Simona Roxana | Benea, Vasile | Ene, Corina Daniela | Tampa, Mircea | Mitran, Cristina Iulia | Nicolae, Ilinca | Pribac, George Ciprian | Prisca, Mirandolina | Ursoiu, Fulvia | Neamtu, Carmen | Totolici, Bogdan | Cotoraci, Coralia | Ardelean, Aurel | Albu, Simona Elena | Carsote, Mara | Miclăuș, Beatrice | Mihai, Diana | Săndulescu, Oana | Vasiliu, Cristina | Vasiliu, Cristina | Carsote, Mara | Gorgoi, Corina | Miclăuș, Beatrice | Mihai, Diana | Săndulescu, Oana | Albu, Simona Elena | Blescun, Amelia | Breaza, Gelu | Vintila, Sabina | Mihai, Felicia | Omer, Meilin | Dragan, Cornel | Pitigoi, Daniela | Ciucu, Mirela | Ionescu, Marius-Dan | Roskanovic, Cristina | Barbu, Valentina | Diaconescu, Iulian | Dumitrescu, Florentina | Niculescu, Irina | Ionică, Mihaela | Zamfir, Ramona-Alexandra | Cozma, Alina | Benea, Otilia Elisabeta | Dumitru, Alexandra-Sînziana | Munteanu, Daniela-Ioana | Niță, Violeta | Popescu, Cristina | Bodosca, Iulia | Tenita, Angelica | Ispas, Viorica | Aramă, Victoria | Benea, Vasile | Georgescu, Simona Roxana | Tampa, Mircea | Leahu, Diana Oana | Safta, Cristina Maria | Benea, Mihaela Anca | Săndulescu, Oana | Munteanu, Octavian | Bohâlțea, Roxana | Trașcă, Livia | Cîrstoiu, Monica | Iovănescu, Doina Viorica | Roșculeț, Cleo Nicoleta | Rogoz, Andrei | Apostolescu, Cătălin Gabriel | Mitescu, Viorica Daniela | Vladoiu, Tudor Gheorghe | Toma, Dalila | Ciuca, Catrinel | Georgescu, Mădălina | Pițigoi, Daniela | Ivanciuc, Alina Elena | Lazar, Mihaela | Ionescu, Teodora | Cherciu, Carmen Maria | Țecu, Cristina | Mihai, Maria Elena | Nițescu, Maria | Bacruban, Rodica | Azamfire, Delia | Dumitrescu, Aura | Ianosik, Elena | Leca, Daniela | Duca, Elena | Teodor, Andra | Bejan, Codrina | Ceaușu, Emanoil | Florescu, Simin-Aysel | Popescu, Corneliu | Târdei, Grațiela | Juganariu, Codrina | Lupulescu, Emilia | Rodina, Ligia | Cocuz, Maria Elena | Jugulete, Gheorghiță | Stăncescu, Adina | Popescu, Cristina Elena | Marin, Luminița | Zaharia, Diana | Dumitrescu, Cristina | Osman, Endis | Niculescu, Irina | Cupșa, Augustin | Diaconescu, Iulian | Dumitrescu, Florentina | Dragonu, Livia | Stoian, Andreea | Giubelan, Lucian | Roskanovic, Cristina | Zamfir, Ramona-Alexandra | Ionica, Mihaela | Benea, Otilia-Elisabeta | Sîrbu, Maria-Cristina | Dobrotă, AnaMaria | Neguț, Alina Cristina | Duda, Roxana | Bacruban, Rodica | Pițigoi, Daniela | Dragomirescu, Cristiana Cerasella | Tălăpan, Daniela | Dorobăț, Olga | Streinu-Cercel, Adrian | Streinu-Cercel, Anca | Ionica, Mihaela | Zamfir, Ramona-Alexandra | Cozma, Alina | Benea, Otilia Elisabeta | Fendrihan, Sergiu | Scortan, Ecaterina | Popa, Mircea Ioan | Popescu, Corneliu P. | Benea, Șerban N. | Petcu, Andra E. | Hristea, Adriana | Abagiu, Adrian | Podea, Iuliana A. | Jipa, Raluca E. | Ducu, Georgeta | Hrișcă, Raluca M. | Florea, Dragoș | Nica, Manuela | Manea, Eliza | Merișor, Simona | Nicolae, Cristian M. | Florescu, Simin A. | Dumitru, Irina M. | Ceaușu, Emanoil | Rugină, Sorin | Moroti, Ruxandra V. | Pițigoi, Daniela | Ionescu, Teodora | Săndulescu, Oana | Nițescu, Maria | Nițescu, Bogdan | Mustaţă, Iulia Monica | Boldeanu, Sorina Claudia | Furtunescu, Florentina | Streinu-Cercel, Adrian | Iacob, Diana Gabriela | Iacob, Simona Alexandra | Gheorghe, Mihaela | Slavcovici, Adriana | Tripon, Raluca | Iubu, Roxana | Marcu, Cristian | Sabou, Mihaela | Muntean, Monica | Chiriac, Ion | Holban, Tiberiu | Tazlavanu, Liviu | Jipa, Raluca | Manea, Eliza | Cernat, Roxana | Iringo, Kezdi | Vâță, Andrei | Arbune, Manuela | Moisil, Teodora | Hristea, Adriana | Ene, Corina-Daniela | Nicolae, Ilinca | Georgescu, Roxana Simona | Ene, Corina-Daniela | Ene, Cosmin-Victor | Georgescu, Roxana Simona | Ciortea, Marilena | Dulgheru, Lucreția | Nicolae, Ilinca | Luca, Mihaela Cătălina | Harja-Alexa, Ioana-Alina | Nemescu, Roxana | Popazu, Mădălina | Luca, Andrei Ștefan | Bancescu, Gabriela | Dabu, Bogdan | Bancescu, Adrian | Manea, Eliza | Jipa, Raluca | Hristea, Adriana | Ilie, Adina Elena | Pohrib, Săftica-Mariana | Neguț, Alina Cristina | Tache, Maria-Sabina | Moțoi, Maria Magdalena | Săndulescu, Oana | Iliescu, Ion Aurel | Streinu-Cercel, Adrian | Tecu, Cristina | Mihai, Maria-Elena | Lazăr, Mihaela | Cherciu, Carmen | Ivanciuc, Alina | Pițigoi, Daniela | Lupulescu, Emilia | Paliu, Mirela | Curescu, Manuela | Cerbu, Bianca | Marincu, Iosif | Mihai, Maria Elena | Cherciu, Carmen Maria | Ivanciuc, Alina Elena | Tecu, Cristina | Lupulescu, Emilia | Bunescu, Irina | Holban, Tiberiu | Pasnin, Ana | Semeniuc, Stela | Popovici, Raisa | Chiriacov, Galina
BMC Infectious Diseases  2016;16(Suppl 4):31-76.
Table of contents
A1 The outcome of patients with recurrent versus non-recurrent pneumococcal meningitis in a tertiary health-care hospital in Bucharest
Cristian-Mihail Niculae, Eliza Manea, Raluca Jipa, Simona Merisor, Ruxandra Moroti, Serban Benea, Adriana Hristea
A2 Influence of bacteriophages on sessile Gram-positive and Gram-negative bacteria
Alina Cristina Neguț, Oana Săndulescu, Anca Streinu-Cercel, Dana Mărculescu, Magdalena Lorena Andrei, Veronica Ilie, Marcela Popa, Coralia Bleotu, Carmen Chifiriuc, Mircea Ioan Popa, Adrian Streinu-Cercel
A3 The utility of inflammatory biomarkers in the prognostic evaluation of septic patients – past, present and future
Alina Orfanu, Cristina Popescu, Anca Leuștean, Remulus Catană, Anca Negru, Alexandra Badea, Radu Orfanu, Cătălin Tilișcan, Victoria Aramă, Ştefan Sorin Aramă
A4 Etiologic and clinical features of bacterial meningitis in infants
Constanța-Angelica Vișan, Anca-Cristina Drăgănescu, Anuța Bilașco, Camelia Kouris, Mădălina Merișescu, Magdalena Vasile, Diana-Maria Slavu, Sabina Vintilă, Endis Osman, Alina Oprea, Sabina Sandu, Monica Luminos
A5 The diagnostic and prognostic role of neutrophil to lymphocyte count ratio in sepsis
Alina Orfanu, Victoria Aramă, Ştefan Sorin Aramă, Anca Leuştean, Remulus Catană, Anca Negru, Gabriel Adrian Popescu, Cristina Popescu
A6 Whooping cough in a HIV positive patient
Ramona Georgiana Stanculete, Ana Vaduva Enoiu, Adelina Raluca Marinescu, Voichita Lazureanu
A7 Cronobacter sakazakii sepsis in varicella patient
Adelina-Raluca Marinescu, Alexandru Crișan, Voichița Lăzureanu, Virgil Musta, Narcisa Nicolescu, Ruxandra Laza
A8 Anaerobes an underdiagnosed cause of prosthesis joint infection
Anca-Ruxandra Negru, Daniela-Ioana Munteanu, Raluca Mihăilescu, Remulus Catană, Olga Dorobăț, Alexandru Rafila, Emilia Căpraru, Marius Niculescu, Rodica Marinescu, Olivera Lupescu, Vlad Predescu, Adrian Streinu-Cercel, Victoria Aramă, Daniela Tălăpan
A9 Streptococcus pneumoniae meningitis presenting with normal CSF – case presentation
Ramona Ștefania Popescu, Luminița Bradu, Dragoș Florea, Adrian Streinu-Cercel
A10 Extrapulmonary manifestations of infection with Mycoplasma pneumoniae – study on 24 cases
Daniela Anicuta Leca, Elena Bunea, Andra Teodor, Egidia Miftode
A11 The molecular diagnosis of severe bacterial sepsis in pediatric population
Mădălina Merișescu, Gheorghiță Jugulete, Adrian Streinu-Cercel, Dragoș Florea, Monica Luminos
A12 Acute Staphylococcus aureus endocarditis with multiple septic complications in a patient with diabetes mellitus – case presentation
Ramona Ștefania Popescu, Anamaria Dobrotă, Adina Ilie, Liliana Lucia Preoțescu
A13 Is Streptococcus suis meningitis an under-diagnosed zoonosis?
Adriana Hristea, Raluca Jipa, Nicoleta Irimescu, Irina Panait, Eliza Manea, Simona Merisor, Cristian Niculae, Daniela Tălăpan
A14 Klebsiella pneumoniae isolated from blood. Antimicrobial resistance – past and present
Liana Cătălina Gavriliu, Otilia Elisabeta Benea, Șerban Benea, Alexandru Rafila, Olga Dorobăț, Mona Popoiu
A15 Antibiotics resistance in Staphylococcus aureus isolated from blood cultures
Livia Dragonu, Augustin Cupşa, Iulian Diaconescu, Irina Niculescu, Lucian Giubelan, Florentina Dumitrescu, Andreea Cristina Stoian, Camelia Guţă, Simona Puiu
A16 Predominance of CTX-M enzymes in extended-spectrum β-lactamase-producing Enterobacteriaceae in two hospitals of Quebec City
Bunescu Irina, Marilyse Vallée, Ann Huletsky, Dominique K. Boudreau, Ève Bérubé, Richard Giroux, Jean Longtin, Yves Longtin, Michel G. Bergeron
A17 Postoperative meningoencephalitis with Acinetobacter baumannii XDR – a therapeutic challenge - Case report
Cleo Nicoleta Roșculeț, Dalila-Ana Toma, Catrinel Ciuca, Daniela Tălăpan, Cătălin Apostolescu, Andrei Rogoz, Andrei Stangaciu, Viorica Mitescu, Tudor Vladoiu, Doina Iovănescu
A18 Septic arthritis with Burkholderia cepacia
Michaela Oana, Simona Costin
A19 A novel approach for managing hard-to-treat infections
Alina Cristina Neguț, Oana Săndulescu, Anca Streinu-Cercel, Maria Magdalena Moțoi, Mircea Ioan Popa, Adrian Streinu-Cercel
A20 Nineteen months surveillance for multidrug resistant organisms (MDRO) by detecting asymptomatic colonization
Daniela Tălăpan, Olga Mihaela Dorobăț, Mona Popoiu, Alexandru Mihai, Doina Iovănescu, Cleo Roşculeț, Cătălin Apostolescu, Gabriel-Adrian Popescu, Adrian Abagiu, Ruxandra Moroti-Constantinescu, Adriana Hristea, Victoria Aramă, Otilia Benea, Mădălina Simoiu, Rodica Bacruban, Adrian Streinu-Cercel, Alexandru Rafila
A21 Antimicrobial resistance of Gram-positive cocci isolated from clinical specimens in the National Institute of Infectious Diseases “Prof Dr. Matei Balș” between 2009–2015
Olga Mihaela Dorobăț, Daniela Tălăpan, Alexandru Mihai, Ioana Bădicuț, Mona Popoiu, Alina Borcan, Alexandru Rafila
A22 The high percentage of carbapenem-resistant Gram-negative bacilli in Romania: an analysis and some proposals
Gabriel Adrian Popescu
A23 Etiological, clinical and therapeutic considerations on 78 cases of healthcare associated meningitis or ventriculitis admitted in the “Sf. Parascheva” infectious diseases clinical hospital, Iași, from 2011 to 2015
Mihnea Hurmuzache, Georgiana Enache, Alexandra Ciocan, Mircea Bararu, Madalina Popazu
A24 Nosocomial infection dynamics in an Intensive Care Department – an overview (epidemiological and clinical monitoring, advanced therapeutic intervention).
Doina Viorica Iovănescu, Cleo Nicoleta Roșculeț, Andrei Rogoz Cătălin Gabriel Apostolescu, Viorica Mitescu, Tudor Vladoiu, Dalila Toma, Catrinel Ciuca
A25 Safety and efficacy of interferon free treatment in patients with HCV chronic hepatitis- experience of a single Internal Medicine center
Laura Iliescu, Georgiana Minzala, Letitia Toma, Mihaela Baciu, Alina Tanase, Carmen Orban
A26 Viusid in treatment of chronic viral hepatitis B and C
Victor Pantea, Gheorghe Placinta, Valentin Cebotarescu, Lilia Cojuhari, Paulina Jimbei
A27 The management of hyperbilirubinemia in HCV cirrhotic patients who underwent therapy with direct acting antivirals
Cristina Popescu, Anca Leuștean, Cristina Dragomirescu, Alina Orfanu, Cristina Murariu, Laurențiu Stratan, Alexandra Badea, Cătălin Tilișcan, Daniela Munteanu, Raluca Năstase, Violeta Molagic, Mihaela Rădulescu, Remulus Catana, Victoria Aramă
A28 The efficacy of ombitasvir-paritaprevir/ritonavir, dasabuvir and ribavirin in patients with genotype 1 HCV compensated cirrhosis
Cristina Popescu, Laurențiu Stratan, Remulus Catana, Anca Leuștean, Cristina Dragomirescu, Alexandra Badea, Cristina Murariu, Raluca Năstase, Violeta Molagic, Daniela Munteanu, Cătălin Tilișcan, Mihaela Rădulescu, Alina Orfanu, Ioan Diaconu, Anca Negru, Iulia Bodosca, Violeta Niță, Victoria Aramă
A29 The efficacy of direct acting antivirals regimen without ribavirin in HCV genotype 1b infected patients with compensated cirrhosis
Anca Leuștean, Victoria Aramă, Alina Orfanu, Remulus Catana, Laurențiu Stratan, Cristina Dragomirescu, Cristina Murariu, Alexandra Badea, Cătălin Tilișcan, Daniela Munteanu, Violeta Molagic, Raluca Năstase, Mihaela Rădulescu, Cristina Popescu
A30 Liver decompensation during ombitasvir-paritaprevir/ritonavir-dasabuvir and ribavirin regimen in HCV infected patients with Child-Pugh A cirrhosis
Cristina Popescu, Cristina Dragomirescu, Anca Leuștean, Cristina Murariu, Laurențiu Stratan, Alexandra Badea, Remulus Catană, Alina Orfanu, Raluca Mihaela Năstase, Violeta Molagic, Daniela Munteanu, Cătălin Tilișcan, Victoria Aramă
A31 The safety of direct acting antivirals in HCV compensated cirrhotic patients - an interim analysis
Victoria Aramă, Remulus Catană, Cristina Dragomirescu, Cristina Murariu, Anca Leuștean, Laurențiu Stratan, Alexandra Badea, Alina Orfanu, Anca Negru, Raluca Năstase, Violeta Molagic, Daniela Munteanu, Cătălin Tilișcan, Mihaela Rădulescu, Ioan Diaconu, Violeta Niță, Iulia Bodoșca, Cristina Popescu
A32 The access of patients with HCV compensated cirrhosis to the National Program of therapy with direct acting antivirals
Cristina Popescu, Alexandra Badea, Anca Leuștean, Alina Orfanu, Anca Negru, Laurențiu Stratan, Cristina Dragomirescu, Remulus Catană, Cristina Murariu, Violeta Molagic, Raluca Năstase, Cătălin Tilișcan, Daniela Munteanu, Mihaela Rădulescu, Ioan Diaconu, Violeta Niță, Iulia Bodoșca, Victoria Aramă
A33 Severe reactivation of chronic hepatitis B after discontinuation of nucleos(t)ide analogues – a case series
Cristina Popescu, Alina Orfanu, Anca Leuștean, Alexandra Badea, Laurențiu Stratan, Remulus Catană, Cătălin Tilișcan, Victoria Aramă
A34 The dynamic of hematological disorders during direct acting antivirals therapy for HCV compensated cirrhosis
Cristina Popescu, Cristina Murariu, Cristina Dragomirescu, Anca Leuștean, Laurențiu Stratan, Alina Orfanu, Alexandra Badea, Remulus Catană, Anca Negru, Cătălin Tilișcan, Daniela Munteanu, Mihaela Rădulescu, Violeta Molagic, Raluca Mihaela Năstase, Ioan Alexandru Diaconu, Iulia Bodoșca, Violeta Niță, Victoria Aramă
A35 Behaviors, attitudes and risk factors for viral hepatitis in international medical students vs. the general population in Romania
Yagmur Erturk, Oana Săndulescu, Alina Cristina Neguț, Claudiu Mihai Șchiopu, Adrian Streinu-Cercel, Anca Streinu-Cercel
A36 Characteristics of hepatitis C virus reactivation due to immunosuppressive therapy in Romanian HCV infected patients with hematological malignancies
Violeta Molagic, Cătălin Tilișcan, Cristina Popescu, Raluca Mihăilescu, Daniela Munteanu, Raluca Năstase, Anca Negru, Angelica Tenita, Victoria Aramă, Ștefan Sorin Aramă
A37 The dynamic IFN-gamma serum levels during successful peginterferon-a 2a/ribavirin therapy in HCV chronic infection
Simona Alexandra Iacob, Diana Gabriela Iacob, Monica Luminos
A38 Overlapping risk factors for transmission of HBV, HCV and HIV in the general population in Romania
Anca Streinu-Cercel, Oana Săndulescu, Mioara Predescu, Alexandra Mărdărescu, Cătălin Tilișcan, Mihai Săndulescu, Claudiu Mihai Șchiopu, Adrian Streinu-Cercel
A39 Acute hepatitis - an uncommon neurological complication
Cleo Nicoleta Roșculeț, Catrinel Olimpia Ciuca, Dalila Ana Toma, Cătălin Gabriel Apostolescu, Andrei Rogoz, Cristina Elena Mitu, Andrei Stangaciu, Viorica Daniela Mitescu, Tudor Gheorghe Vladoiu, Doina Viorica Iovănescu
A40 Regression of liver fibrosis following sustained virological response in patients with chronic HCV infection and cirrhosis
Oana Săndulescu, Anca Streinu-Cercel, Monica Andreea Stoica, Liliana Lucia Preoțescu, Daniela Manolache, Gabriela Jana Ceapraga, Maria Magdalena Moțoi, Luminița Bradu, Adina Ilie, Gabriela Mircea, Ionel Durbală, Adrian Streinu-Cercel
A41 Preliminary results of treatment with sofosbuvir and daclatasvir of patients with chronic hepatitis C
Irina Russu, Tiberiu Holban, Tatiana Pantilimonov, Galina Chiriacov, Arcadie Macvovei, Elena Scorohodico, Oleg Dmitriev
A42 HIV-syphilis coinfection
Diana Alexandra Costache, Anca Benea, Eliza Manea, Cristian Niculae, Raluca Jipa, Adriana Hristea, Elisabeta Benea, Ruxandra Moroti, Șerban Benea
A43 Thrombophilia – additional risk factor for the evolution of pregnancy in HIV-positive patients
Mihai Mitran, Carmen Georgescu, Loredana Mitran, Simona Vladareanu
A44 The incidence of oropharyngeal candidiasis in hospitalized HIV infected pediatric Romanian cohort between 1 January - 31 December 2015
Andreea Ioana Magirescu, Viorica Andreev, Cristina Nicolau, Alexandra Largu, Carmen Dorobat, Carmen Manciuc
A45 TB incidence in HIV infected patients during the year of 2015
Viorica Andreev, Andreea Ioana Magirescu, Ina Isac, Cristina Nicolau, Alexandra Largu, Carmen Dorobat, Carmen Manciuc
A46 Retrospective analysis of HIV/AIDS deaths recorded in the Clinical Infectious Diseases Hospital, Constanța in the period 01 January 2014–30 June 2016. Epidemiological considerations.
Iulia Gabriela Șerban, Ghiulendan Resul, Consuela Marcaș
A47 Acute liver failure with favorable evolution in an HIV-HBV coinfected patient
Iosif Marincu, Patricia Poptelecan, Bogdan Trincă, Sorina Mitrescu, Anca Tudor, Daliborca Vlad, Livius Tirnea
A48 Lifestyle impact on HIV management
Nurcan Baydaroglu, Alina Cristina Neguț, Oana Săndulescu, Daniela Manolache, Gabriela Ceapraga, Monica Andreea Stoica, Anca Streinu-Cercel, Adrian Streinu-Cercel
49. HIV positive mothers newborns - clinical experience from January 2012 to June 2016
Carmen Manciuc, Mariana Pagute, Cristina Nicolau, Carmen Dorobăț, Alexandra Largu
A50 Rediscovering HIV-associated progressive multifocal leukoencephalopathy and HIV encephalopathy: clinical suspicion and subsequent brain autopsies
Ioan-Alexandru Diaconu, Laurențiu Stratan, Daniela Ion, Luciana Nichita, Cristina Popescu, Raluca Năstase, Daniela Munteanu, Violeta Molagic, Cătălin Tilișcan, Mihaela Rădulescu, Alexandra Diaconu, Anca Negru, Alina Orfanu, Cristina Dragomirescu, Remulus Catană, Anca Leuștean, Irina Duport-Dodot, Cristina Murariu, Iulia Bodoșca, Violeta Niță, Alexandra Badea, Victoria Aramă
A51 Antenatal surveillance of pregnant women with risk behavior and its impact on mother-to-child HIV transmission in Romania
Mariana Mărdărescu, Cristina Petre, Marieta Iancu, Rodica Ungurianu, Alina Cibea, Ruxandra Drăghicenoiu, Ana Maria Tudor, Delia Vlad, Sorin Petrea, Carina Matei, Dan Oțelea, Carmen Crăciun, Cristian Anghelina, Alexandra Mărdărescu
A52 Noninvasive assessments (APRI, Fib-4, transient elastography) of fibrosis in patients with HIV and HIV/HBV infection
Elena Dumea, Adrian Streinu-Cercel, Sorin Rugină, Lucian Cristian Petcu, Stela Halichidis, Simona Claudia Cambrea
A53 Undetectable HIV viral load – the main goal in the management of HIV-infected patients
Carmen Chiriac, Nina-Ioana Bodnar, Iringo-Erzsebet Zaharia-Kezdi, Cristina Gîrbovan, Andrea Incze, Anca Meda Georgescu
A54 LPS serum levels and correlation with immunological, virological and clinical outcome in HIV infected patients
Simona Alexandra Iacob, Diana Gabriela Iacob, Eugenia Panaitescu, Monica Luminos, Manole Cojocaru
A55 LL37 human cathelicidin serum levels are positively correlated with IFN gamma and alanine aminotransferase level in HCV infection
Simona Alexandra Iacob, Diana Gabriela Iacob, Monica Luminos
A56 Early diagnosis of pulmonary tuberculosis in a non-compliant HIV/AIDS late presenter patient
Vochita Laurențiu, Vochita Andreia, Opreanu Radu, Trinca Bogdan, Rosca Ovidiu, Marincu Iosif
A57 Evolution of antiretroviral regimens in naϊve patients in 2016
Ramona Zamfir, Alina Angelescu, Alena Andreea Popa, Raluca Jipa, Ruxandra Moroti, Adriana Hristea, Liana Gavriliu, Șerban Benea, Elisabeta Benea
A58 The unfavorable risk factors for HIV infected persons with positive blood cultures hospitalized at the National Institute for Infectious Diseases “Prof. Dr. Matei Balș” in 2015
Alena-Andreea Popa, Georgeta Ducu, Daniela Camburu, Alina Cozma, Manuela Podani, Roxana Dumitriu, Liana Gavriliu, Șerban Benea, Elisabeta Benea
A59 Epidemiological aspects of HIV infection in Oltenia region
Andreea Cristina Stoian, Florentina Dumitrescu, Augustin Cupșa, Lucian Giubelan, Irina Niculescu, Loredana Ionescu, Livia Dragonu
A60 HIV risk behaviors and prevalence among patients in methadone maintenance therapy (MMT) from Arena center, Bucharest
Adrian Octavian Abagiu, Loredana Nicoleta Stoica, Catrinel Blaga, Archontis Koulosousas, Roxana Ștefănescu, Alice Atomoaie, Florentina Paraschiv, Florin Matache Duna
A61 Therapeutic options in a case of severe psoriasis associated with both HIV infection and hepatitis C virus previously treated with fumaric acid esters
Rodica Olteanu, Roxana Ion, Alexandra Zota, Isra Ennour Jaballah, Lara Mahfoud, Georgeta Preda, Magda Constantin
A62 Prevalence of autoantibodies against gangliosides in asymptomatic HIV-infected patients
Ilinca Nicolae, Corina Daniela Ene, Mădălina Irina Mitran, Vasile Benea, Mircea Tampa, Simona Roxana Georgescu
A63 Subclinical inflammation in HIV-infected patients undergoing antiretroviral therapy – a cross sectional study
Iulia Cristina Bodoșca, Cristina Murariu, Cătălin Tilișcan, Victoria Aramă, Cristina Popescu, Daniela Munteanu, Mihaela Rădulescu, Violeta Molagic, Raluca Năstase, Alina Orfanu, Anca Leuștean, Remulus Catană, Anca Negru, Adrian Streinu-Cercel, Sorin Aramă
A64 Severe Guillain-Barré syndrome occurring after chickenpox with favorable evolution
Iuliana CAramăngiu, Ovidiu Rosca, Monica Cialma, Radu Opreanu, Laurențiu Vochita, Iosif Marincu
A65 Echovirus 30 infection with pulmonary and cardiac complications – case report
Vlad Murărescu, Marilena Palaghiță, Alina Cristina Neguț, Cornel Camburu, Adrian Streinu-Cercel
A66 Herpetic encephalitis with favorable evolution in an adult immunocompetent patient
Irina Duşan, Patricia Poptelecan, Bogdan Trincă, Sorina Mitrescu, Livius Tirnea, Iosif Marincu
A67 Clinical-evolutional aspects in present-day measles
Narcisa Nicolescu, Alexandru Crișan, Voichița Lăzureanu, Ruxandra Laza, Virgil Musta, Adelina-Raluca Marinescu, Andreea Bîrlad
A68 Pneumococcal superinfection in children with influenza
Victor Daniel Miron, Anca Cristina Drăgănescu, Constanța-Angelica Vișan, Anuța Bilașco, Daniela Pițigoi, Oana Săndulescu, Monica Luminița Luminos
A69 Varicella complicated with transverse myelitis - case presentation
Monica Luminos, Endis Osman, Magdalena Vasile, Anca Cristina Drăgănescu, Constanța-Angelica Vișan, Anuța Bilașco, Camelia Kouris, Sabina Șchiopu, Mădălina Merișescu
A70 Clinical forms of enterovirus infections during the summer season of 2016
Monica Luminos, Anca Cristina Drăgănescu, Constanța-Angelica Vișan, Anuța Bilașco, Camelia Kouris, Endis Osman, Sabina Vintilă, Magda Vasile, Mădălina Merișescu
A71 Face off – HIV and lymphoma – case series presentation
Liana Cătălina Gavriliu, Otilia Elisabeta Benea, Alina Angelescu, Ramona Zamfir, Daniela Camburu, Georgeta Ducu, Alina Cozma, Roxana Dumitriu, Manuela Podani, Șerban Benea, Mihaela Ionică
A72 Coxsackie infection complicated by pancytopenia – pediatric case report
Gheorghiță Jugulete, Adina Stăncescu, Cristina Elena Popescu, Luminița Marin, Diana Zaharia, Cristina Dumitrescu, Lucia Tudor, Sabina Vintilă
A73 Viral respiratory infections in children in the season 2015–2016
Constanța-Angelica Vișan, Anca Cristina Drăgănescu, Anuța Bilașco, Magda Vasile, Mădălina Merișescu, Camelia Kouris, Cristina Negulescu, Endis Osman, Diana-Maria Slavu, Sabina Vintilă, Daniela Pițigoi, Monica Luminos
A75 The severity of A H1N1 Influenza infection in the 2015–2016 season
Cleo Roșculeț, Catrinel Olimpia Ciuca, Dalila Toma, Cătălin Apostolescu, Andrei Rogoz, Andrei Stangaciu, Viorica Mitescu, Doina Iovănescu, Cornel Camburu, Bogdana Manu
A76 Acute respiratory distress syndrome in a child with measles
Ana Vaduva-Enoiu, Ramona Georgiana Stanculete, Adelina Raluca Marinescu, Voichita Elena Lazureanu
A77 Management challenges of right-sided infectious endocarditis in an HIV positive patient – case presentation
Elena-Violeta Niță, Sînziana Dumitru, Daniela-Ioana Munteanu, Anca Ruxandra Negru, Remulus Catană, Ioan Diaconu, Bogdana Manu, Ligia Ionescu, Liliana Ion, Cătălin Tilișcan, Victoria Aramă
A78 Bacterial infection in critical patients with severe A H1N1 influenza virus infection (epidemiology, development, therapeutic decisions)
Doina Viorica Iovănescu, Cleo Nicoleta Roșculeț, Andrei Rogoz, Cătălin Apostolescu, Viorica Mitescu, Tudor Vladoiu, Dalila Toma, Catrinel Ciuca
A79 Epidemiological aspects of severe acute respiratory infection cases (SARI) in the season 2015–2016, in the Clinical Hospital of Infectious Diseases – Constanța, Romania
Iulia Gabriela Șerban, Marioara Neacșu
A80Overexpression of IL-6 trans signaling pathway in viral infections
Simona Roxana Georgescu, Vasile Benea, Corina Daniela Ene, Mircea Tampa, Cristina Iulia Mitran, Ilinca Nicolae
A81 Acute viral hepatitis B with persistent HBsAg – description and evolution
George Ciprian Pribac, Mirandolina Prisca, Fulvia Ursoiu, Carmen Neamtu, Bogdan Totolici, Coralia Cotoraci, Aurel Ardelean
A82 Prevalence of cervical pathogens in a population of pregnant female patients monitored in a tertiary care hospital in Bucharest, Romania
Simona Elena Albu, Mara Carsote, Beatrice Miclăuș, Diana Mihai, Oana Săndulescu, Cristina Vasiliu
A83 Prevalence of group B Streptococcus during pregnancy in a cohort of patients monitored in a tertiary care hospital in Bucharest, Romania
Cristina Vasiliu, Mara Carsote, Corina Gorgoi, Beatrice Miclăuș, Diana Mihai, Oana Săndulescu, Simona Elena Albu
A84 Infectious hematoma in the gastrocnemius muscle – case presentation
Amelia Blescun, Gelu Breaza
A85 Reflections towards the underexplored HTLV Romanian viral circulation - adult T‐cell leukemia/lymphomas, a case series
Sabina Vintila, Felicia Mihai, Meilin Omer, Cornel Dragan, Daniela Pitigoi
A86 A febrile confusion syndrome with acute onset – case presentation
Mirela Ciucu, Marius-Dan Ionescu, Cristina Roskanovic, Valentina Barbu, Iulian Diaconescu, Florentina Dumitrescu, Irina Niculescu
A87 Retrobulbar optic neuritis in a HIV-positive patient - case report
Mihaela Ionică, Ramona-Alexandra Zamfir, Alina Cozma, Otilia Elisabeta Benea
A88 A rare presentation of Q fever – case presentation
Alexandra-Sînziana Dumitru, Daniela-Ioana Munteanu, Violeta Niță, Cristina Popescu, Iulia Bodosca, Angelica Tenita, Viorica Ispas, Victoria Aramă
A89 Tinea incognita – case presentation
Vasile Benea, Simona Roxana Georgescu, Mircea Tampa, Diana Oana Leahu, Cristina Maria Safta, Mihaela Anca Benea
A90 Incidence and risk factors associated with TORCH infections during pregnancy
Oana Săndulescu, Octavian Munteanu, Roxana Bohâlțea, Livia Trașcă, Monica Cîrstoiu
A91 Acute respiratory failure in critical patients with sepsis
Doina Viorica Iovănescu, Cleo Nicoleta Roșculeț, Andrei Rogoz, Cătălin Gabriel Apostolescu, Viorica Daniela Mitescu, Tudor Gheorghe Vladoiu, Dalila Toma, Catrinel Ciuca
A92 Cochleo-vestibular deficit secondary to Granulicatella elegans meningitis
Mădălina Georgescu
A93 Influenza 2015/2016 – clinical, epidemiological and virological characteristics of cases admitted in three infectious diseases hospitals
Daniela Pițigoi, Alina Elena Ivanciuc, Mihaela Lazar, Teodora Ionescu, Carmen Maria Cherciu, Cristina Țecu, Maria Elena Mihai, Maria Nițescu, Rodica Bacruban, Delia Azamfire, Aura Dumitrescu, Elena Ianosik, Daniela Leca, Elena Duca, Andra Teodor, Codrina Bejan, Emanoil Ceaușu, Simin-Aysel Florescu, Corneliu Popescu, Grațiela Târdei, Codrina Juganariu, Emilia Lupulescu
A94 Severe complications of varicella requiring hospitalization in previously healthy children in Brașov county
Ligia Rodina, Maria Elena Cocuz
A95 Clinical forms of Clostridium difficile colitis in children
Gheorghiță Jugulete, Adina Stăncescu, Cristina Elena Popescu, Luminița Marin, Diana Zaharia, Cristina Dumitrescu, Endis Osman
A96 Community-acquired pneumonia – demographic, clinical and etiological aspects
Irina Niculescu, Augustin Cupșa, Iulian Diaconescu, Florentina Dumitrescu, Livia Dragonu, Andreea Stoian, Lucian Giubelan, Cristina Roskanovic
A97 Acute myocarditis in an adult patient with chickenpox - Case report
Ramona-Alexandra Zamfir, Mihaela Ionica, Otilia-Elisabeta Benea
A98 Caustic oropharyngeal wound with acute group F streptococcal superinfection mimicking diphtheria – case report and differential diagnosis
Maria-Cristina Sîrbu, AnaMaria Dobrotă, Alina Cristina Neguț, Roxana Duda, Rodica Bacruban, Daniela Pițigoi, Cristiana Cerasella Dragomirescu, Daniela Tălăpan, Olga Dorobăț, Adrian Streinu-Cercel, Anca Streinu-Cercel
A99 Clostridium difficile infection in HIV-positive patients admitted in the National Institute for Infectious Diseases “Prof. Dr. Matei Balș” in 2015
Mihaela Ionica, Ramona-Alexandra Zamfir, Alina Cozma, Otilia Elisabeta Benea
A100 Title: Epidemiology of Candida oral infections (stomatitis) in Romania
Sergiu Fendrihan, Ecaterina Scortan, Mircea Ioan Popa
A101 Anthrax case series in south-eastern Romania
Corneliu P Popescu, Șerban N Benea, Andra E Petcu, Adriana Hristea, Adrian Abagiu, Iuliana A Podea, Raluca E Jipa, Georgeta Ducu, Raluca M Hrișcă, Dragoș Florea, Manuela Nica, Eliza Manea, Simona Merișor, Cristian M Nicolae, Simin A Florescu, Irina M Dumitru, Emanoil Ceaușu, Sorin Rugină, Ruxandra V Moroti
A102 Knowledge, risk perception and attitudes of healthcare workers at the National Institute for Infectious Diseases “Prof. Dr. Matei Balș” regarding Ebola
Daniela Pițigoi, Teodora Ionescu, Oana Săndulescu, Maria Nițescu, Bogdan Nițescu, Iulia Monica Mustaţă, Sorina Claudia Boldeanu, Florentina Furtunescu, Adrian Streinu-Cercel
A103 A case of abdominopelvic actinomycosis with successful short-term antibiotic treatment
Diana Gabriela Iacob, Simona Alexandra Iacob, Mihaela Gheorghe
A104 A case of pneumonia caused by Raoultella planticola
Iulian Diaconescu, Irina Niculescu, Floretina Dumitrescu, Lucian Giubelan
A105 Vitamin D deficiency and sepsis in childhood
Adriana Slavcovici, Raluca Tripon, Roxana Iubu, Cristian Marcu, Mihaela Sabou, Monica Muntean
A106 The clinical and epidemiological aspects and prophylaxis of Lyme disease among patients who presented with tick bites to the Clinical Infectious Disease Hospital “Toma Ciorbă”
Ion Chiriac, Tiberiu Holban, Liviu Tazlavanu
A107 Drug-resistant tuberculosis in HIV infected patients
Raluca Jipa, Eliza Manea, Roxana Cernat, Kezdi Iringo, Andrei Vâță, Manuela Arbune, Teodora Moisil, Adriana Hristea
A108 Kidney injury molecule-1 and urinary tract infections
Corina-Daniela Ene, Ilinca Nicolae, Roxana Simona Georgescu
A109 The impact of microbiological agents on serum gangliosides in patients with benign prostate hyperplasia
Corina-Daniela Ene, Cosmin-Victor Ene, Roxana Simona Georgescu, Marilena Ciortea , Lucreția Dulgheru, Ilinca Nicolae
A110 Toxocariasis - the experience of the Iași Infectious Diseases Hospital between 2013–2015
Mihaela Cătălina Luca, Ioana-Alina Harja-Alexa, Roxana Nemescu, Mădălina Popazu, Andrei Ștefan Luca
A111 Species of anaerobic Gram-positive cocci involved in odontogenic abscesses
Gabriela Bancescu, Bogdan Dabu, Adrian Bancescu
A112 Clostridium difficile infection recurrences
Eliza Manea, Raluca Jipa, Adriana Hristea
A113 Differential diagnosis of staphylococcal and tuberculous osteodiscitis – case report
Adina Elena Ilie, Săftica-Mariana Pohrib, Alina Cristina Neguț, Maria-Sabina Tache, Maria Magdalena Moțoi, Oana Săndulescu, Ion Aurel Iliescu, Adrian Streinu-Cercel
A114 Severe clinical forms of respiratory syncytial virus infections
Cristina Tecu, Maria-Elena Mihai, Mihaela Lazăr, Carmen Cherciu, Alina Ivanciuc, Daniela Pițigoi, Emilia Lupulescu
A115 Acinetobacter baumannii postoperative sepsis associated with Clostridium difficile enterocolitis in an immune suppressed elderly patient
Mirela Paliu, Manuela Curescu, Bianca Cerbu, Iosif Marincu
A116 Risk factors and their impact on psychopathology and quality of life among people living with HIV/AIDS in Romania
Fulvia Ursoiu, Mirandolina Prișcă, George Ciprian Pribac
A117 Antivirals susceptibility of influenza viruses circulating in Romania
Maria Elena Mihai, Carmen Maria Cherciu, Alina Elena Ivanciuc, Cristina Tecu, Emilia Lupulescu
A118 Retrospective study of hospitalized cases of sepsis at the Hospital Clinic of Infectious Diseases “Toma Ciorbă”
Irina Bunescu, Tiberiu Holban, Ana Pasnin, Stela Semeniuc, Raisa Popovici, Galina Chiriacov
doi:10.1186/s12879-016-1877-4
PMCID: PMC5103241
23.  Intraperitoneal Fat through GRP78: A Risk Factor for Endometrial Cancer 
Introduction. The identification of biological markers that indicate an increased risk for the development or recurrence of endometrial cancer (EC) in obese women might be useful for decreasing EC mortality and morbidity. Glucose-regulated protein 78 (GRP78) is a major protein of the endoplasmic reticulum expressed in all normal cells. Overexpression of GRP78 has been reported to be a tumoral biomarker. Increased detection of GRP78 is positively correlated with the tumoral stage and prognosis. This study aimed to identify a correlation between intraperitoneal fat, plasma GRP78 levels, and EC. Materials and Methods. Two groups of patients were included in the study: group I, 44 patients diagnosed with EC, and group II, 44 patients without gynecological pathology or inflammatory disorders. Visceral fat was determined by ultrasound and plasma GRP78 levels were measured. Results. Plasma GRP78 levels were significantly higher in patients with EC compared to the control group. Intraperitoneal fat was in a positive linear correlation with the plasma GRP78 level (p < 0.0001). Conclusion. The measurement of the GRP78 level associated with the determination of intraperitoneal fat can be a useful predictor for EC.
doi:10.1155/2016/3496538
PMCID: PMC5086347  PMID: 27822448
24.  Lysis of HIV-1 Infected Autologous CD4+ Primary T cells by Interferon-alpha Activated NK cells Requires NKp46 and NKG2D 
AIDS (London, England)  2015;29(14):1767-1773.
Objective
Autologous HIV-1 infected CD4+ primary T cells (aHIV+CD4) have been shown to be largely resistant to Natural Killer (NK) cell mediated lysis due to viral strategies of immune evasion. We have previously shown that a pre-activation of NK cells with Plasmacytoid Dendritic Cells can significantly augment lysis of aHIV+CD4 through a mechanism dependent on Interferon-alpha (IFN-α).
Design
The goal of the present study is to identify the specific NK activating receptors involved in NK lysis of aHIV+CD4 following IFN-α activation.
Methods
PBMC were incubated with aHIV+CD4 to induce the secretion of endogenous levels of IFN-α and drive NK activation. We then utilized a standard chromium lysis assay to assess the degree of IFN-α activated lysis of aHIV+CD4 in the presence or absence of masking antibodies to a panel of NK activating receptors and co-receptors.
Results
Direct recognition of HIV-1 infected, but not uninfected, autologous CD4+ primary T cells by PBMC induced the secretion IFN-α (Median 2280 pg/ml, p<0.001, n=9) that, in turn, activated NK cells (p<0.001, n=12) and significantly increased their cytolytic potential against aHIV+CD4 (p<0.01, n=12). The masking of NKp46 (p<0.01, n=8) and NKG2D (p<0.05, n=8), but not 2B4, NTBA, NKp30 or NKp44, significantly reduced IFN-α activated lysis of aHIV+CD4.
Conclusions
Taken together, these results demonstrate that endogenous levels of IFN-α secreted by pDCs induce NK cells to lyse aHIV+CD4 via the engagement of NKp46 and NKG2D.
doi:10.1097/QAD.0000000000000777
PMCID: PMC4571461  PMID: 26372382
AIDS; NK Cells; NKp46; NKG2D; Interferon-alpha; Cytotoxicity
25.  Reciprocal Regulation of Endocytosis and Metabolism 
The cellular uptake of many nutrients and micronutrients governs both their cellular availability and their systemic homeostasis. The cellular rate of nutrient or ion uptake (e.g., glucose, Fe3+, K+) or efflux (e.g., Na+) is governed by a complement of membrane transporters and receptors that show dynamic localization at both the plasma membrane and defined intracellular membrane compartments. Regulation of the rate and mechanism of endocytosis controls the amounts of these proteins on the cell surface, which in many cases determines nutrient uptake or secretion. Moreover, the metabolic action of diverse hormones is initiated upon binding to surface receptors that then undergo regulated endocytosis and show distinct signaling patterns once internalized. Here, we examine how the endocytosis of nutrient transporters and carriers as well as signaling receptors governs cellular metabolism and thereby systemic (whole-body) metabolite homeostasis.
The endocytosis of nutrient carriers (e.g., LDLR), transporters (e.g., GLUTs), and signaling receptors (e.g., IR) controls cellular metabolism and systemic metabolite homeostasis.
doi:10.1101/cshperspect.a016964
PMCID: PMC4067987  PMID: 24984778

Results 1-25 (139)