PMCCPMCCPMCC

Conseils de recherche
Les critères de recherche 

Avancée
Logo of actafjournal home pageInternational Union of Crystallographysearchsubscribearticle submissionthis issue
 
Cette revue donne accès immédiatement à certains articles

Volume 69(Pt 12);  2013 décembre 1

Editorial

Manfred S. Weiss, Howard Einspahr

The Editors of Acta F look forward to 2014.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1315. Published online 2013 November 30. doi: 10.1107/S1744309113031990
PMCID:
PMC3855710

Structural Communications

Andrei S. Halavaty, Keith Moffat

The X-ray structure of the light, oxygen or voltage domain 2 of phototropin 1 from A. thaliana (AtLOV2) in its dark-adapted state has been determined. The N-terminal flanking A′α helix of AtLOV2 plays a role in its dimerization.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1316–1321. Published online 2013 November 28. doi: 10.1107/S1744309113029199
PMCID:
PMC3855711
Stéphanie Baelen, Frédérique Dewitte, Bernard Clantin, Vincent Villeret

The structure of the secretion domain of HxuA from H. influenzae has been determined to 1.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1322–1327. Published online 2013 November 28. doi: 10.1107/S174430911302962X
PMCID:
PMC3855712
N. Schormann, S. Banerjee, R. Ricciardi, D. Chattopadhyay

The crystal structure of the uracil complex of Vaccinia virus uracil DNA glycosylase (D4) has been determined at 2.03 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1328–1334. Published online 2013 November 28. doi: 10.1107/S1744309113030613
PMCID:
PMC3855713
Markus Alahuhta, William S. Adney, Michael E. Himmel, Vladimir V. Lunin

The 1.82 Å resolution X-ray structure of a thermally tolerant glycoside hydrolase family 74 enzyme from A. cellulolyticus is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1335–1338. Published online 2013 November 28. doi: 10.1107/S1744309113030005
PMCID:
PMC3855714
Vadim Rimsa, Thomas C. Eadsforth, William N. Hunter

Two structures of human Siah1 at 1.95 and 1.58 Å resolution provide more complete models for this protein and identify conformational variability in the subdomain organization.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1339–1343. Published online 2013 November 28. doi: 10.1107/S1744309113031448
PMCID:
PMC3855715
Aiko Fujino, Kei Fukushima, Takaharu Kubota, Yoshiyuki Matsumoto, Midori Takimoto-Kamimura

The glycine-rich loop of MK2 (MAPKAP-K2) bound to the non-selective inhibitor TEI-L03090 adopts the β-form, which is commonly found in other kinase complexes; however, it differs from the α-form which is adopted when MK2 is bound to the MK2-selective inhibitor TEI-I01800. These results show how conformational change of MK2 influences its selectivity.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1344–1348. Published online 2013 November 28. doi: 10.1107/S1744309113030534
PMCID:
PMC3855716

Crystallization Communications

Parveen Goyal, Nani Van Gerven, Wim Jonckheere, Han Remaut

Structural insights into the type-VIII protein secretion system are described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1349–1353. Published online 2013 November 28. doi: 10.1107/S1744309113028054
PMCID:
PMC3855717
Ai-Fen Chai, Jodie M. Johnston, Richard D. Bunker, Esther M. M. Bulloch, Genevieve L. Evans, J. Shaun Lott, Edward N. Baker

MbtN (Rv1346) from M. tuberculosis was cloned, overexpressed, purified and crystallized. The crystals diffracted to 2.3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1354–1356. Published online 2013 November 28. doi: 10.1107/S1744309113027000
PMCID:
PMC3855718
Masaki Unno, Saya Kinjo, Kenji Kizawa, Hidenari Takahara

Human peptidylarginine deiminase type I was crystallized using polyethylene glycol as a precipitant. The crystals belonged to space group P61, with unit-cell parameters a = b = 90.3, c = 372.3 Å, and contained two molecules in the asymmetric unit.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1357–1359. Published online 2013 November 28. doi: 10.1107/S1744309113028704
PMCID:
PMC3855719
Takayuki Ohnuma, Naoyuki Umemoto, Toki Taira, Tamo Fukamizo, Tomoyuki Numata

A selenomethionine-labelled active-site mutant of ‘loopless’ family GH19 chitinase from B. coronatum has been co-crystallized with chitotetraose. X-ray diffraction data were collected to 1.58 Å resolution using a synchrotron-radiation source.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1360–1362. Published online 2013 November 28. doi: 10.1107/S1744309113028935
PMCID:
PMC3855720
Carmela Garcia-Doval, Daniel Luque, José R. Castón, Pascale Boulanger, Mark J. van Raaij

The trimeric L-shaped fibre protein from bacteriophage T5 has been analysed by electron microscopy and a C-terminal domain has been crystallized. Diffraction data have been obtained for two native crystal forms and one selenomethionine derivative.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1363–1367. Published online 2013 November 28. doi: 10.1107/S1744309113028959
PMCID:
PMC3855721
Shintaro Maeda, Kyoko Shinzawa-Itoh, Kaoru Mieda, Mami Yamamoto, Yumiko Nakashima, Yumi Ogasawara, Chimari Jiko, Kazutoshi Tani, Atsuo Miyazawa, Christoph Gerle, Shinya Yoshikawa

Two-dimensional crystallization conditions for intact bovine F-ATP synthase have been established.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1368–1370. Published online 2013 November 29. doi: 10.1107/S1744309113029072
PMCID:
PMC3855722
Sheng Ding, Ruiqing Zhou, Yaqin Zhu

The chimeric protein LKB1–14-3-3ζ was purified and crystallized. The X-ray diffraction were collected to a resolution of 2.9–Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1371–1373. Published online 2013 November 29. doi: 10.1107/S1744309113029230
PMCID:
PMC3855723
Abhimanyu K. Singh, Rosa Menéndez-Conejero, Carmen San Martín, Mark J. van Raaij

A C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus, has been crystallized. Diffraction data have been obtained to high resolution for several native crystal forms and a selenomethionine derivative.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1374–1379. Published online 2013 November 29. doi: 10.1107/S1744309113029308
PMCID:
PMC3855724
Nur Zazarina Ramly, Sergey N. Rouzheinikov, Svetlana E. Sedelnikova, Patrick J. Baker, Yock-Ping Chow, Kiew-Lian Wan, Sheila Nathan, David W. Rice

Recombinant SAG19 from E. tenella has been overexpressed, purified and crystallized in a form suitable for X-ray analysis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1380–1383. Published online 2013 November 29. doi: 10.1107/S1744309113029734
PMCID:
PMC3855725
Tatyana A. Sysoeva, Neela Yennawar, Marc Allaire, B. Tracy Nixon

This study reports the crystallization of a new nucleotide state of the ATPase domain of a bacterial transcription activator NtrC1 from the hyperthermophilic bacterium Aquifex aeolicus. Wild-type NtrC1 ATPase domain was crystallized in the presence of the ATP analog ADP–BeFx–Mg and the crystals diffracted anisotropically to at best 3.2, 5.2 and 3.2 Å resolution in the

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1384–1388. Published online 2013 November 29. doi: 10.1107/S174430911302976X
PMCID:
PMC3855726
Natalia Pakharukova, Minna Tuittila, Anton Zavialov

Donor-strand complemented AggA subunit of aggregative adherence fimbriae type I from Shiga toxin-producing E. coli O104:H4 was crystallized. Diffraction data were collected to 1.55 Å resolution. Initial phases were derived from a sulfur single anomalous diffraction experiment.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1389–1392. Published online 2013 November 29. doi: 10.1107/S1744309113029990
PMCID:
PMC3855727
Marta Sanz-Gaitero, Ruth Keary, Carmela Garcia-Doval, Aidan Coffey, Mark J. van Raaij

The N-terminal cysteine, histidine-dependent amidohydrolase (peptidase) domain of the S. aureus bacteriophage K endolysin LysK has been crystallized. Native and derivative data to a resolution of 1.7–1.8 Å have been obtained, which allowed structure solution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1393–1396. Published online 2013 November 29. doi: 10.1107/S1744309113030133
PMCID:
PMC3855728
Takamitsu Furuyama, Yoshikazu Ishii, Norimasa Ohya, Kazuhiro Tateda, Nancy D. Hanson, Akiko Shimizu-Ibuka

The class B carbapenemase IMP-18 from P. aeruginosa was overexpressed in E. coli, purified and crystallized. The crystals belonged to space group P41212 and diffracted to 2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1397–1400. Published online 2013 November 29. doi: 10.1107/S1744309113030480
PMCID:
PMC3855729
Dilip C. Badgujar, Ulka Sawant, Vikrant, Lumbini Yadav, M. V. Hosur, Ashok K. Varma

The crystallization and preliminary X-ray diffraction analysis of BRCA1 BRCT with ABRAXAS phosphopeptide are reported. Binding affinity using ITC has been compared.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1401–1404. Published online 2013 November 29. doi: 10.1107/S1744309113030649
PMCID:
PMC3855730
Beatriz Trastoy, Joseph V. Lomino, Lai-Xi Wang, Eric J. Sundberg

A comparative study of vapor diffusion versus liquid–liquid diffusion methods used for the crystallization of EndoS is reported. X-ray diffraction data to 2.6 and 1.9 Å resolution were collected for wild-type endoglycosidase and glycosynthase constructs of EndoS, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1405–1410. Published online 2013 November 29. doi: 10.1107/S1744309113030650
PMCID:
PMC3855731
Masahiko Kato, Yuichiro Kezuka, Chihoko Kobayashi, Kazuhiro Ishibashi, Takamasa Nonaka, Masayuki Ishikawa, Estuko Katoh

A crystal of Tm-1, which is an inhibitor protein of Tomato mosaic virus RNA replication, was obtained using the hanging-drop vapour-diffusion method at 293 K and diffracted X-rays to 2.7 Å resolution. It belonged to space group P1, with unit-cell parameters a = 77.97, b = 105.28, c = 110.62 Å, α = 94.6, β = 109.3, γ = 108.0°.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1411–1414. Published online 2013 November 29. doi: 10.1107/S1744309113030819
PMCID:
PMC3855732
Maren Thomsen, Lilly Skalden, Gottfried J. Palm, Matthias Höhne, Uwe T. Bornscheuer, Winfried Hinrichs

The (R)-selective amine transaminase from A. fumigatus was purified and crystallized. Diffraction data to 1.84 Å resolution for S-SAD phasing as well as high-resolution data to 1.27 Å were collected from a single crystal belonging to space group C2221.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1415–1417. Published online 2013 November 29. doi: 10.1107/S1744309113030923
PMCID:
PMC3855733
Agnesa Shala, Kevin H. Patel, Dasantila Golemi-Kotra, Gerald F. Audette

The expression, purification, crystallization and preliminary X-ray diffraction analysis of the N-terminal receiver domain of LytR, LytRN, from S. aureus is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1418–1421. Published online 2013 November 29. doi: 10.1107/S1744309113030972
PMCID:
PMC3855734
A. Paul, A. Mishra, A. Surolia, M. Vijayan

Argininosuccinate lyase from M. tuberculosis has been cloned, purified and crystallized, and preliminary X-ray studies on the crystals have been carried out.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1422–1424. Published online 2013 November 29. doi: 10.1107/S1744309113031138
PMCID:
PMC3855735
Alba Ruiz-Ramos, Nada Lallous, Araceli Grande-García, Santiago Ramón-Maiques

The recombinant aspartate transcarbamoylase domain of human CAD was expressed in E. coli, purified and crystallized in the presence and absence of the inhibitor PALA. X-ray diffraction data sets were collected for both crystal forms at 2.1 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1425–1430. Published online 2013 November 29. doi: 10.1107/S1744309113031114
PMCID:
PMC3855736
Miriam Rico-Jiménez, Francisco Muñoz-Martínez, Tino Krell, Jose A. Gavira, Estela Pineda-Molina

To determine the molecular basis underlying the ligand specificity of the ligand-binding regions of Pseudomonas chemotactic transducer A and B, complexes with amino acids have been purified and crystals have been produced.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1431–1435. Published online 2013 November 29. doi: 10.1107/S1744309113023592
PMCID:
PMC3855737
Jing Huang, Tong Wu, Zheng Guo, Tiantian Lou, Shaoning Yu, Weimin Gong, Chaoneng Ji

The Escherichia coli cyclic AMP receptor protein (CRP) is a prokaryotic global transcription activator protein that controls the expression of many different genes. cAMP-free D53H CRP crystals were obtained and diffracted at a resolution of 2.9 Å. Based on the systematic absences of the crystals, the likely space group is P212121 with the unit-cell parameters a = 76.66, b = 152.14,

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1436–1439. Published online 2013 November 29. doi: 10.1107/S174430911303145X
PMCID:
PMC3855738
Anil Kumar Verma, Arun Goyal, Filipe Freire, Pedro Bule, Immacolata Venditto, Joana L. A. Brás, Helena Santos, Vânia Cardoso, Cecília Bonifácio, Andrew Thompson, Maria João Romão, José A. M. Prates, Luís M. A. Ferreira, Carlos M. G. A. Fontes, Shabir Najmudin

The glycoside hydrolase family 30 module from the cellulosomal C. thermocellum XynGH30 was purified and crystallized and data were collected to 1.4 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 December 1; 69(Pt 12): 1440–1442. Published online 2013 November 30. doi: 10.1107/S1744309113025050
PMCID:
PMC3855739

Les articles de Acta Crystallographica Section F: Structural Biology and Crystallization Communications ont été offerts à titre gracieux par International Union of Crystallography.