This journal provides early access to some articles.
Volume 68(Pt 7); July 1, 2012
Paul K. Fyfe, Gareth D. Westrop, Tania Ramos, Sylke Müller, Graham H. Coombs, William N. Hunter
A crystallographic and biochemical study of L. major cysteine synthase, which is a pyridoxyl phosphate-dependent enzyme, is reported. The structure was determined to 1.8 Å resolution and revealed that the cofactor has been lost and that a fragment of γ-poly-d-glutamic acid, a crystallization ingredient, was bound in the active site. The enzyme was inhibited by peptides.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 738–743. Published online 2012 June 22. doi: 10.1107/S1744309112019124
Benjamin Stieglitz, Katrin Rittinger, Lesley F. Haire
The expression, purification and crystallization of an N-terminal fragment of SHARPIN are reported. Diffraction-quality crystals were obtained using a two-dimensional grid-screen seeding technique.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 816–819. Published online 2012 June 28. doi: 10.1107/S1744309112022208
Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
Mads Gabrielsen, M. Florencia Rey-Burusco, Kate Griffiths, Andrew J. Roe, Alan Cooper, Brian O. Smith, Malcolm W. Kennedy, Betina Corsico
Na-FAR-1, a fatty acid- and retinol-binding protein, was expressed in bacteria, purified and crystallized. Crystals grew in two different morphologies under the same conditions.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 835–838. Published online 2012 June 28. doi: 10.1107/S1744309112023597