This journal provides early access to some articles.
Volume 68(Pt 7); July 1, 2012
Paul K. Fyfe, Gareth D. Westrop, Tania Ramos, Sylke Müller, Graham H. Coombs, William N. Hunter
A crystallographic and biochemical study of L. major cysteine synthase, which is a pyridoxyl phosphate-dependent enzyme, is reported. The structure was determined to 1.8 Å resolution and revealed that the cofactor has been lost and that a fragment of γ-poly-d-glutamic acid, a crystallization ingredient, was bound in the active site. The enzyme was inhibited by peptides.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 738–743. Published online 2012 June 22. doi: 10.1107/S1744309112019124
Crystallization and X-ray diffraction studies of arginine kinase from the white Pacific shrimp Litopenaeus vannamei
Alonso A. Lopez-Zavala, Rogerio R. Sotelo-Mundo, Karina D. Garcia-Orozco, Felipe Isac-Martinez, Luis G. Brieba, Enrique Rudiño-Piñera
The crystallization and preliminary X-ray diffraction analysis at 1.25 Å resolution of free-ligand arginine kinase from the Pacific whiteleg shrimp Litopenaeus vannamei are reported. Crystals belong to space group P212121, phases were determined by molecular replacement and refinement was performed with Phenix.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 783–785. Published online 2012 June 27. doi: 10.1107/S1744309112020180
Benjamin Stieglitz, Katrin Rittinger, Lesley F. Haire
The expression, purification and crystallization of an N-terminal fragment of SHARPIN are reported. Diffraction-quality crystals were obtained using a two-dimensional grid-screen seeding technique.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 816–819. Published online 2012 June 28. doi: 10.1107/S1744309112022208
Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus
Mads Gabrielsen, M. Florencia Rey-Burusco, Kate Griffiths, Andrew J. Roe, Alan Cooper, Brian O. Smith, Malcolm W. Kennedy, Betina Corsico
Na-FAR-1, a fatty acid- and retinol-binding protein, was expressed in bacteria, purified and crystallized. Crystals grew in two different morphologies under the same conditions.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 835–838. Published online 2012 June 28. doi: 10.1107/S1744309112023597
Dustin J. Little, John C. Whitney, Howard Robinson, Patrick Yip, Mark Nitz, P. Lynne Howell
Construct engineering and crystallization of E. coli PgaB using in situ proteolysis and mass spectrometry is reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 842–845. Published online 2012 June 28. doi: 10.1107/S1744309112022075