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Volume 68(Pt 7);  2012 July 1

Structural Communications

Sarah Sainsbury, Jingshan Ren, Nigel J. Saunders, David I. Stuart, Raymond J. Owens

The crystal structure of the regulatory domain of NMB2055, a putative MetR regulator, was solved at 2.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 730–737. Published online 2012 June 22. doi: 10.1107/S1744309112010603
PMCID:
PMC3388910
Paul K. Fyfe, Gareth D. Westrop, Tania Ramos, Sylke Müller, Graham H. Coombs, William N. Hunter

A crystallographic and biochemical study of L. major cysteine synthase, which is a pyridoxyl phosphate-dependent enzyme, is reported. The structure was determined to 1.8 Å resolution and revealed that the cofactor has been lost and that a fragment of γ-poly-d-glutamic acid, a crystallization ingredient, was bound in the active site. The enzyme was inhibited by peptides.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 738–743. Published online 2012 June 22. doi: 10.1107/S1744309112019124
PMCID:
PMC3388911
Kim Henrick, Miriam Hirshberg

The 1.85 Å resolution structure of the signal transduction protein TRAP is presented. The overall fold of TRAP is an unsymmetrical eight-stranded β-barrel with five helices.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 744–750. Published online 2012 June 22. doi: 10.1107/S1744309112020167
PMCID:
PMC3388912
Sebastian Himmel, Christian Grosse, Sebastian Wolff, Claudia Schwiegk, Stefan Becker

The crystal structure of the RBD-PRDI fragment of the antiterminator protein GlcT from Bacillus subtilis has been solved at 2 Å resolution. The structure represents an inactive state of the protein.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 751–756. Published online 2012 June 22. doi: 10.1107/S1744309112020635
PMCID:
PMC3388913

Crystallization Communications

Hiroaki Nakano, Allin Hosokawa, Ryuji Tagawa, Koji Inaka, Kazunori Ohta, Toru Nakatsu, Hiroaki Kato, Kunihiko Watanabe

In this work, Pz peptidase B, an intracellular M3 metallopeptidase that is found in the thermophile Geobacillus collagenovorans MO-1, was crystallized using the counter-diffusion method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 757–759. Published online 2012 June 27. doi: 10.1107/S1744309112018969
PMCID:
PMC3388914
Xueyong Xu, Xiaoyan Wang, Jingjin Ding, Da-Cheng Wang

The complex of CCM3 and the C-terminal domain of MST4 has been successfully constructed, purified and crystallized. The crystal diffracted to a resolution of 2.4 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 760–763. Published online 2012 June 27. doi: 10.1107/S1744309112016843
PMCID:
PMC3388915
Xing Zhou, Yue Tao, Minhao Wu, Dandan Zhang, Jianye Zang

The JmjC domain-containing histone demethylase NO66 from H. sapiens was overproduced in E. coli, purified and crystallized. Diffraction data were collected to 2.29 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 764–766. Published online 2012 June 27. doi: 10.1107/S174430911201740X
PMCID:
PMC3388916
Shang-ke Chen, Kui Wang, Yuhuan Liu, Xiaopeng Hu

A novel feruloyl esterase (EstF27) identified from a soil metagenomic library has been crystallized and a complete data set was collected from a single cooled crystal using an in-house X-ray source.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 767–770. Published online 2012 June 27. doi: 10.1107/S1744309112017812
PMCID:
PMC3388917
Geraldine Buysschaert, Kenneth Verstraete, Savvas N. Savvides, Bjorn Vergauwen

An atypical short-chain dehydrogenase/reductase from Vibrio vulnificus was expressed, purified and cocrystallized with NADPH by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected to 1.80 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 771–774. Published online 2012 June 27. doi: 10.1107/S1744309112018672
PMCID:
PMC3388918
Ye Yuan, Xiao Wang, Xu Li, Maikun Teng, Liwen Niu, Yongxiang Gao

This paper reports the cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of acyl-protein thioesterase 1 from S. cerevisiae.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 775–777. Published online 2012 June 27. doi: 10.1107/S1744309112019276
PMCID:
PMC3388919
Kazuki Kato, Hiroshi Nishimasu, Emiko Mihara, Ryuichiro Ishitani, Junichi Takagi, Junken Aoki, Osamu Nureki

The extracellular region of mouse Enpp1 was expressed, purified and crystallized. An X-ray diffraction data set was collected to 3.0 Å resolution by employing a helical data-collection strategy involving a micro-focus synchrotron beam.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 778–782. Published online 2012 June 27. doi: 10.1107/S1744309112019306
PMCID:
PMC3388920
Alonso A. Lopez-Zavala, Rogerio R. Sotelo-Mundo, Karina D. Garcia-Orozco, Felipe Isac-Martinez, Luis G. Brieba, Enrique Rudiño-Piñera

The crystallization and preliminary X-ray diffraction analysis at 1.25 Å resolution of free-ligand arginine kinase from the Pacific whiteleg shrimp Litopenaeus vannamei are reported. Crystals belong to space group P212121, phases were determined by molecular replacement and refinement was performed with Phenix.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 783–785. Published online 2012 June 27. doi: 10.1107/S1744309112020180
PMCID:
PMC3388921
Debajyoti Dutta, Sudipta Bhattacharyya, Amit Kumar Das

The cloning, overexpression, purification, crystallization in a triclinic space group and preliminary X-ray diffraction analysis of the high-molecular-weight ketoacyl reductase FabG4 complexed with NADH are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 786–789. Published online 2012 June 27. doi: 10.1107/S1744309112020301
PMCID:
PMC3388922
Kayla Baretta, Craig Garen, Jiang Yin, Michael N. G. James

Approximately five decades have passed with only one or two new antibiotics making it into clinical use. Phosphoglycerate kinase from A. baumanii has been selected as a potential target for antibiotic development; this paper presents the initial structural biological results from this research.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 790–792. Published online 2012 June 27. doi: 10.1107/S1744309112020222
PMCID:
PMC3388923
Christian Linke, Nikolai Siemens, Martin J. Middleditch, Bernd Kreikemeyer, Edward N. Baker

The putative adhesion domain of the multidomain protein Epf from S. pyogenes has been crystallized in space groups P21 and P212121. The crystals diffracted to 2.0 and 1.6 Å resolution, respectively, at the Australian Synchrotron.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 793–797. Published online 2012 June 28. doi: 10.1107/S1744309112020313
PMCID:
PMC3388924
Xinghua Qin, Ziwei Chen, Tao Xu, Ping Li, Guoqin Liu

The GhKCH2 motor domain was crystallized and the pH of the crystallization buffer was shown to have a significant effect on the crystal morphology and diffraction quality.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 798–801. Published online 2012 June 28. doi: 10.1107/S1744309112016351
PMCID:
PMC3388925
Lei Han, Zheng Liu, Xinqi Liu, Dewen Qiu

The overexpression, purification, crystallization and preliminary X-ray diffraction analysis of protein elicitor PevD1 from Verticillium dahliae are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 802–805. Published online 2012 June 28. doi: 10.1107/S1744309112020556
PMCID:
PMC3388926
Hackwon Do, Jun Hyuck Lee, Sung Gu Lee, Hak Jun Kim

The ice-binding protein FfIBP from F. frigoris PS1 was overexpressed, purified, characterized and crystallized, and preliminary X-ray crystallographic analysis was performed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 806–809. Published online 2012 June 28. doi: 10.1107/S1744309112020465
PMCID:
PMC3388927
Chi-Hung Huang, Chao-Hung Kao, Chia-Shin Yang, Chi-Huang Chang, Sheng-Chia Chen, Shu-Min Kuan, Yen-Chao Su, Yu-Han Huang, Ming-Chung Chang, Yeh Chen

The S. epidermidis carrier protein DltC has been crystallized in order to elucidate the functional role of DltC in the alanylation of lipoteichoic acids in bacteria.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 810–812. Published online 2012 June 28. doi: 10.1107/S1744309112021720
PMCID:
PMC3388928
Hye Jin Kim, Eunha Hwang, Young-Hyun Han, Saehae Choi, Woo Cheol Lee, Hye-Yeon Kim, Young Ho Jeon, Chaejoon Cheong, Hae-Kap Cheong

The crystallization of the human NORE1 SARAH domain is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 813–815. Published online 2012 June 28. doi: 10.1107/S1744309112021744
PMCID:
PMC3388929
Benjamin Stieglitz, Katrin Rittinger, Lesley F. Haire

The expression, purification and crystallization of an N-terminal fragment of SHARPIN are reported. Diffraction-quality crystals were obtained using a two-dimensional grid-screen seeding technique.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 816–819. Published online 2012 June 28. doi: 10.1107/S1744309112022208
PMCID:
PMC3388930
Neta Tanner, Gali Prag

The yeast Epsin-1 (ent1) gene has been cloned and expressed in Escherichia coli. The protein product of a construct containing the ENTH-UIM modules was purified to homogeneity and subjected to crystallization screening using the sitting-drop vapour-diffusion method. Refined conditions containing polyethylene glycol 3350 and Tacsimate yielded thin rod-like crystals.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 820–823. Published online 2012 June 28. doi: 10.1107/S1744309112022488
PMCID:
PMC3388931
Laura A. Lallemand, James G. McCarthy, Sean McSweeney, Andrew A. McCarthy

A hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase involved in chlorogenic acid biosynthesis in C. canephora was crystallized using the vapour-diffusion method. A diffraction data set was collected to 3.0 Å resolution on the microfocus beamline (ID23-2) at ESRF and a structure solution was obtained using molecular replacement.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 824–828. Published online 2012 June 28. doi: 10.1107/S1744309112019082
PMCID:
PMC3388932
Kai Deng, Feng Gao, Peng Zheng, Weimin Gong, Zhe Sun

The purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of δ-COP, a medium-sized subunit of the COPI complex, are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 829–831. Published online 2012 June 28. doi: 10.1107/S1744309112022798
PMCID:
PMC3388933
Michelle L. Tonkin, Shannon Brown, Josh R. Beck, Peter J. Bradley, Martin J. Boulanger

To characterize the ISP family of proteins present in apicomplexan parasites, ISP1 from T. gondii was expressed, purified and crystallized. Two crystal forms (cubic and orthorhombic) were analyzed by X-ray diffraction and data were processed to 2.05 and 2.1 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 832–834. Published online 2012 June 28. doi: 10.1107/S174430911202297X
PMCID:
PMC3388934
Mads Gabrielsen, M. Florencia Rey-Burusco, Kate Griffiths, Andrew J. Roe, Alan Cooper, Brian O. Smith, Malcolm W. Kennedy, Betina Corsico

Na-FAR-1, a fatty acid- and retinol-binding protein, was expressed in bacteria, purified and crystallized. Crystals grew in two different morphologies under the same conditions.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 835–838. Published online 2012 June 28. doi: 10.1107/S1744309112023597
PMCID:
PMC3388935
Haitian Fan, Wei Sun, Zhe Sun, Feng Gao, Weimin Gong

A protease involved in turnover of a photosynthesis reaction centre protein has been crystallized and preliminary diffraction analysis was performed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 839–841. Published online 2012 June 28. doi: 10.1107/S1744309112023603
PMCID:
PMC3388936
Dustin J. Little, John C. Whitney, Howard Robinson, Patrick Yip, Mark Nitz, P. Lynne Howell

Construct engineering and crystallization of E. coli PgaB using in situ proteolysis and mass spectrometry is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 July 1; 68(Pt 7): 842–845. Published online 2012 June 28. doi: 10.1107/S1744309112022075
PMCID:
PMC3388937

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography