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Volume 68(Pt 5);  2012 May 1

Structural Communications

Valerie J. Klema, Bryan J. Johnson, Judith P. Klinman, Carrie M. Wilmot

The structure of copper amine oxidase 1 from H. polymorpha in its metal-free precursor (apo) form is reported along with structures of the apo protein in complex with CuI and CoII.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 501–510. Published online 2012 April 20. doi: 10.1107/S1744309112012857
PMCID:
PMC3374502
Liqin Du, Yu Luo

Hexameric rings of RadA recombinase from M. voltae have been crystallized. Structural comparisons suggest that homologues of RadA tend to form double-ringed assemblies.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 511–516. Published online 2012 April 20. doi: 10.1107/S1744309112010226
PMCID:
PMC3374503
Sangwoo Kim, Akira Nishide, Yasushi Saeki, Kenji Takagi, Keiji Tanaka, Koichi Kato, Tsunehiro Mizushima

A new crystal structure of yeast Rpn14 with an E384A mutation was determined at 1.6 Å resolution. The improved high-resolution structure provides a framework for understanding proteasome assembly.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 517–521. Published online 2012 April 20. doi: 10.1107/S1744309112011359
PMCID:
PMC3374504
Katherine S. H. Beckham, Olwyn Byron, Andrew J. Roe, Mads Gabrielsen

The crystal structure of the C61S mutant of Tpx from E. coli is reported at 1.97 Å resolution. A brief structural comparison with homologues is presented.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 522–526. Published online 2012 April 20. doi: 10.1107/S1744309112011487
PMCID:
PMC3374505
Jacqueline Vitali, Aditya K. Singh, Alexei S. Soares, Michael J. Colaneri

The structure of the catalytic chain of Methanococcus jannaschii aspartate transcarbamoylase has been determined in a hexagonal crystal form and gives insight into the possible paths that the substrate carbamoyl phosphate may follow to reach the active site during catalysis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 527–534. Published online 2012 April 20. doi: 10.1107/S1744309112011037
PMCID:
PMC3374506

Crystallization Communications

Susely F. S. Tada, Antonio Marcos Saraiva, Gabriela S. Lorite, Luciana K. Rosselli-Murai, Alexandre César Pelloso, Marcelo Leite dos Santos, Daniela B. B. Trivella, Mônica A. Cotta, Anete Pereira de Souza, Ricardo Aparicio

Initial crystallographic studies of the X. fastidiosa small heat-shock protein HSP17.9 are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 535–539. Published online 2012 April 20. doi: 10.1107/S1744309112009347
PMCID:
PMC3374507
Yoichi Suwa, Jun Ohtsuka, Takuya Miyakawa, Fabiana Lica Imai, Masahiko Okai, Yoriko Sawano, Yoshihiko Yasohara, Michihiko Kataoka, Sakayu Shimizu, Masaru Tanokura

Carbonyl reductase S1 from C. magnoliae was expressed, purified and crystallized. The crystals obtained diffracted X-rays to 1.90 Å resolution and belonged to space group P6122 or P6522.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 540–542. Published online 2012 April 20. doi: 10.1107/S1744309112011645
PMCID:
PMC3374508
Jianru Pan, Kangjing Zhou, Guangjin Zheng, Shutao Liu, Pingfan Rao

A cell membrane permeable SOD, SOD-TAT fusion protein, was expressed, purified and crystallized. X-ray diffraction data have been collected to 3.2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 543–546. Published online 2012 April 20. doi: 10.1107/S1744309112012031
PMCID:
PMC3374509
Aaron Hakim, Durga Thakral, Darren F. Zhu, Jennifer B. Nguyen

A novel hyperactive antifreeze protein from R. inquisitor (RiAFP) has been overexpressed, purified and crystallized. A complete native X-ray diffraction data set was recorded to 1.3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 547–550. Published online 2012 April 20. doi: 10.1107/S1744309112010421
PMCID:
PMC3374510
Ali Rohman, Niels van Oosterwijk, Bauke W. Dijkstra

3-Ketosteroid Δ1-dehydrogenase from Rhodococcus erythropolis SQ1 was successfully crystallized and its initial structure was solved.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 551–556. Published online 2012 April 20. doi: 10.1107/S1744309112011025
PMCID:
PMC3374511
Ah Reum Han, Hyun Sook Kim, Gye Yoon Cho, Ho Sam Ki, Hwa-Young Kim, Kwang Yeon Hwang

The MsrA domain of MsrAB from H. influenzae was crystallized. X-ray diffraction data were collected to 1.6 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 557–559. Published online 2012 April 20. doi: 10.1107/S1744309112011256
PMCID:
PMC3374512
Bum Han Ryu, Tri Duc Ngo, Eunjin Jang, Sungsoo Kim, Hansol Ju, Kyeong Kyu Kim, T. Doohun Kim

Esterase A from C. crescentus CB15 was crystallized in space group C2221 and diffraction data were collected to a resolution of 1.62 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 560–564. Published online 2012 April 21. doi: 10.1107/S1744309112009992
PMCID:
PMC3374513
Chen Wang, Xuexin Fan, Xiaofang Cao, Xiang Liu, Lanfen Li, Xiaodong Su

Two hypothetical ribose-5-phosphate isomerases from S. mutans have been produced in E. coli and crystallized. The crystals diffracted to high resolutions suitable for crystallographic analyses.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 565–568. Published online 2012 April 21. doi: 10.1107/S1744309112009761
PMCID:
PMC3374514
S. D. Carter, J. N. Barr, T. A. Edwards

The Crimean–Congo haemorrhagic fever virus nucleocapsid protein was expressed in E. coli, purified and crystallized. X-ray diffraction data were collected to 2.1 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 569–573. Published online 2012 April 21. doi: 10.1107/S1744309112009736
PMCID:
PMC3374515
Lei Jin, Michael H. A. Roehrl, Li Xiao, Xiuyun He, Haibin Li, Linhu Ge, Bingyi Shi

A trypsin-resistant catalytic domain of human calcineurin α (A subunit, residues 20–347) was crystallized in space group P21212. An X-ray diffraction data set was collected to 2.87 Å resolution and the structure was solved by molecular replacement.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 574–579. Published online 2012 April 21. doi: 10.1107/S1744309112007890
PMCID:
PMC3374516
Ilona Rissanen, Alice Pawlowski, Karl Harlos, Jonathan M. Grimes, David I. Stuart, Jaana K. H. Bamford

The major capsid proteins VP16 and VP17 of bacteriophage P23-77 have been crystallized using both recombinant and purified virus and preliminary diffraction analyses have been performed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 580–583. Published online 2012 April 21. doi: 10.1107/S1744309112010330
PMCID:
PMC3374517
Francis Wolfram, Kritica Arora, Howard Robinson, Ana Mirela Neculai, Patrick Yip, P. Lynne Howell

The expression, purification and crystallization of the alginate lyase AlgL from P. aeruginosa is described. The crystals diffracted to a resolution of 1.64 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 584–587. Published online 2012 April 21. doi: 10.1107/S1744309112012808
PMCID:
PMC3374518
Stefano Da Vela, Marta Ferraroni, Boris A. Kolvenbach, Eva Keller, Philippe F. X. Corvini, Andrea Scozzafava, Fabrizio Briganti

Crystals of hydroquinone dioxygenase from Sphingomonas sp. strain TTNP3 belonged to space group P21 and contained two heterotetramers in the asymmetric unit related by 222 noncrystallographic symmetry. X-ray data were collected to a maximum resolution of 2.5 Å using a synchrotron source.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 588–590. Published online 2012 April 21. doi: 10.1107/S1744309112012341
PMCID:
PMC3374519
Christian Roth, Stefan R. Kaschabek, Janosch A. D. Gröning, Thomas Handrek, Michael Schlömann, Norbert Sträter

The crystallization and preliminary X-ray diffraction analysis of a novel chloromuconolactone dehalogenase from R. opacus 1CP are described. The oligomeric state was determined based on the self-rotation function.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 591–595. Published online 2012 April 21. doi: 10.1107/S1744309112011888
PMCID:
PMC3374520

Laboratory Communications

Todd W. Geders, Kathryn Gustafson, Barry C. Finzel

The utility of differential scanning fluorimetry for homogeneity assessment and crystallization improvement of PLP-dependent enzymes is demonstrated using the potential drug target BioA from M. tuberculosis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 596–600. Published online 2012 April 21. doi: 10.1107/S1744309112012912
PMCID:
PMC3374521
Wenying Qin, Russell A. Judge, Kenton L. Longenecker, Larry R. Solomon, John E. Harlan

An on-column ligand- and detergent-exchange method was developed to obtain ligand–protein complexes for an adamantane series of compounds with 11β-HSD1 after a variety of other complexation methods had failed. An interesting byproduct of the method was the observation of artificial trimers in the crystal structures.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 601–605. Published online 2012 April 21. doi: 10.1107/S1744309112010172
PMCID:
PMC3374522
Jinguang Huang, Yanmei Gong, Dan Huang, Lesley Haire, Junfeng Liu, Youliang Peng

Addition of protease instead of seeds using a robot can be used to optimize the concentration of protease in in situ proteolysis experiments and has been successfully tested using two proteins.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1; 68(Pt 5): 606–609. Published online 2012 April 24. doi: 10.1107/S174430911201161X
PMCID:
PMC3374523

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography