|Accueil | Aperçu | Revues | Soumettre | Nous Contacter | English|
Development of an ontology for the description of crystallization experiments and results is proposed.
The crystal structure of the cytoplasmic cyclophilin A (CyPA) from the bacterium Azotobacter vinelandii complexed with a synthetic tetrapeptide has been determined by molecular replacement at 2 Å resolution.
Structures of IPMDH from the obligate piezophile S. benthica DB21MT-2 (SbIPMDH) and the nonpiezophile S. oneidensis MR-1 (SoIPMDH) were determined at atmospheric pressure. Comparison of the structures revealed that SbIPMDH is in a more open conformation and has a larger internal cavity volume than SoIPMDH.
Large quantities of recombinant human carboxylesterase 1 have been produced in an economical whole insect larvae system. The crystal structure of this enzyme is essentially identical to that produced by cell culture techniques.
A thermostable penicillin G acylase from A. faecalis has been crystallized in two space groups: C2221 and P41212. X-ray diffraction data were collected to 3.3 and 3.5 Å resolution, respectively.
The PsbP-domain-containing protein PPD6 from A. thaliana was expressed, purified and crystallized.
A purified blue-light-absorbing proteorhodopsin D97N mutant protein (BPR_D97N) has been crystallized using the vapour-diffusion method.
In order to characterize the type IV pili of nontypeable Haemophilus influenzae, an attempt to solve the atomic structure of the major pilin subunit PilA was initiated. A 1.73 Å resolution X-ray diffraction data set was collected from native N-terminally truncated PilA (ΔN-PilA).
Succinic semialdehyde dehydrogenase (SSADH) from S. pyogenes was purified and crystallized. Crystals of native and NAD+-complexed SSADH diffracted to resolutions of 1.6 and 1.7 Å, respectively.
Enoyl-acyl carrier protein reductase (FabK) from S. mutans strain UA159 was cloned, overexpressed, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.40 Å.
Translation elongation factor eEF1A2 was purified to homogeneity from rabbit muscles. Obtained crystals of eEF1A2 diffracted to 2.5 Å resolution and belonged to space group P6122 or P6322.
Casein kinase I-like protein from rice was crystallized. The crystals were found to belong to the monoclinic space group C2, with unit-cell parameters a = 108.83, b = 69.60, c = 55.85 Å, β = 109.47°. The crystals were obtained at 293 K and diffracted to a resolution of 2.0 Å.
Crystals of the XccFimXEAL–c-di-GMP and SeMet-XccFimXEAL–c-di-GMP–XccPilZ complexes from X. campestris diffracted to resolutions of 2.5 and 2.7 Å, respectively.
T. harzianum endoglucanase III was expressed in P. pastoris, purified and crystalized. A native X-ray diffraction data set was collected.
Salmonella FlgA, a periplasmic chaperone essential for flagellar P-ring assembly, has been expressed and purified and the crystals have been characterized by X-ray diffraction.
TPP3 is a class II plant defensin from tomato. Here, the expression, purification, crystallization and preliminary X-ray crystallographic analysis of recombinant TPP3 are reported in order to define its structure and function in relation to other class II plant defensins.
Alginate importer from Sphingomonas sp. A1 is a member of the ABC transporter superfamily that directly transports alginate polysaccharide into the cytoplasm. Crystals of alginate importer in complex with the periplasmic binding protein AlgQ2 diffracted X-rays to 3.3 Å resolution.
The purification, crystallization and MS analysis of a natural surfactant protein from the frog L. vastus are described.
Cocrystallization of ferredoxin and glutamate synthase was successfully carried out and the resulting crystals were demonstrated to be suitable for structural determination of an electron-transfer complex of these two proteins.
A hyperthermostable endoglucanase from P. furiosus expressed as a truncated form without the N-terminal amino acids was crystallized. The crystal diffracted to a resolution of 1.07 Å.
A galactose 1-phosphate uridylyltransferase from a hyperthermophilic archaeon was successfully isolated and crystallized.
The catalytic domain of human Dus2-like enzyme was purified and crystallized, and data were collected to 1.9 Å resolution.
The crystallization conditions and preliminary crystal characterization of the cytoplasmic cyclic nucleotide-binding homology domain from the mouse EAG potassium channel are reported.
The cytoplasmic domain of BRI1-associated kinase 1 from A. thaliana has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.6 Å resolution.
The subclass B3 metallo-β-lactamase SMB-1 was crystallized by the hanging-drop vapour-diffusion method. Two types of crystals belonging to space group P31 were obtained.
The L1 ribosomal protein from B. pseudomallei has been overexpressed, purified and crystallized in a form suitable for X-ray analysis.
The crystallization of Tic22 from P. falciparum is reported.
Residues 38–440 of CdpNPT from A. fumigatus were overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 2.5 Å.
The SH2 domain of the human protein tyrosine kinase Fyn has been expressed, purified and crystallized in the unbound state and in complex with a high-affinity phosphotyrosine peptide.