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Volume 68(Pt 3);  2012 mars 1

Editorial

Howard Einspahr, Manfred S. Weiss

Editorial.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 252. Anglais. Published online 2012 February 29. doi: 10.1107/S174430911200680X
PMCID:
PMC3310523

Scientific Comment

Janet Newman, Evan E. Bolton, Jochen Müller-Dieckmann, Vincent J. Fazio, D. Travis Gallagher, David Lovell, Joseph R. Luft, Thomas S. Peat, David Ratcliffe, Roger A. Sayle, Edward H. Snell, Kerry Taylor, Pascal Vallotton, Sameer Velanker, Frank von Delft

Development of an ontology for the description of crystallization experiments and results is proposed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 253–258. Anglais. Published online 2012 February 15. doi: 10.1107/S1744309112002618
PMCID:
PMC3310524

Structural Communications

Elias Christoforides, Maria Dimou, Panagiotis Katinakis, Kostas Bethanis, Michael Karpusas

The crystal structure of the cytoplasmic cyclophilin A (CyPA) from the bacterium Azotobacter vinelandii complexed with a synthetic tetrapeptide has been determined by molecular replacement at 2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 259–264. Anglais. Published online 2012 February 15. doi: 10.1107/S1744309112000188
PMCID:
PMC3310525
Takayuki Nagae, Chiaki Kato, Nobuhisa Watanabe

Structures of IPMDH from the obligate piezophile S. benthica DB21MT-2 (SbIPMDH) and the nonpiezophile S. oneidensis MR-1 (SoIPMDH) were determined at atmospheric pressure. Comparison of the structures revealed that SbIPMDH is in a more open conformation and has a larger internal cavity volume than SoIPMDH.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 265–268. Anglais. Published online 2012 February 15. doi: 10.1107/S1744309112001443
PMCID:
PMC3310526
Harry M. Greenblatt, Tamara C. Otto, Melanie G. Kirkpatrick, Elena Kovaleva, Susan Brown, George Buchman, Douglas M. Cerasoli, Joel L. Sussman

Large quantities of recombinant human carboxylesterase 1 have been produced in an economical whole insect larvae system. The crystal structure of this enzyme is essentially identical to that produced by cell culture techniques.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 269–272. Anglais. Published online 2012 February 15. doi: 10.1107/S1744309112003326
PMCID:
PMC3310527
Nishant Kumar Varshney, R. Suresh Kumar, Zoya Ignatova, Asmita Prabhune, Archana Pundle, Eleanor Dodson, C. G. Suresh

A thermostable penicillin G acylase from A. faecalis has been crystallized in two space groups: C2221 and P41212. X-ray diffraction data were collected to 3.3 and 3.5 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 273–277. Anglais. Published online 2012 February 15. doi: 10.1107/S1744309111053930
PMCID:
PMC3310528

Crystallization Communications

Michael Hall, Thomas Kieselbach, Uwe H. Sauer, Wolfgang P. Schröder

The PsbP-domain-containing protein PPD6 from A. thaliana was expressed, purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 278–280. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309111042023
PMCID:
PMC3310529
Ning Wang, Meitian Wang, Yanyan Gao, Tingting Ran, Yanli Lan, Jian Wang, Langlai Xu, Weiwu Wang

A purified blue-light-absorbing proteorhodopsin D97N mutant protein (BPR_D97N) has been crystallized using the vapour-diffusion method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 281–283. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309111043612
PMCID:
PMC3310530
Subramaniapillai Kolappan, Erin N. Tracy, Lauren O. Bakaletz, Robert S. Munson, Jr, Lisa Craig

In order to characterize the type IV pili of nontypeable Haemophilus influenzae, an attempt to solve the atomic structure of the major pilin subunit PilA was initiated. A 1.73 Å resolution X-ray diffraction data set was collected from native N-terminally truncated PilA (ΔN-PilA).

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 284–287. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309111043910
PMCID:
PMC3310531
Eun Hyuk Jang, Jong Eun Lim, Young Min Chi, Ki Seog Lee

Succinic semialdehyde dehydrogenase (SSADH) from S. pyogenes was purified and crystallized. Crystals of native and NAD+-complexed SSADH diffracted to resolutions of 1.6 and 1.7 Å, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 288–291. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309111052055
PMCID:
PMC3310532
Tae-O Kim, Dong-Won Im, Ha Yun Jung, Seong Jung Kwon, Yong-Seok Heo

Enoyl-acyl carrier protein reductase (FabK) from S. mutans strain UA159 was cloned, overexpressed, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.40 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 292–294. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309112000115
PMCID:
PMC3310533
A. Yaremchuk, V. F. Shalak, O. V. Novosylna, B. S. Negrutskii, T. Crépin, A. V. El’skaya, M. Tukalo

Translation elongation factor eEF1A2 was purified to homogeneity from rabbit muscles. Obtained crystals of eEF1A2 diffracted to 2.5 Å resolution and belonged to space group P6122 or P6322.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 295–297. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309112000243
PMCID:
PMC3310534
Kyoung Hun Do, Hyun Ho Park

Casein kinase I-like protein from rice was crystallized. The crystals were found to belong to the monoclinic space group C2, with unit-cell parameters a = 108.83, b = 69.60, c = 55.85 Å, β = 109.47°. The crystals were obtained at 293 K and diffracted to a resolution of 2.0 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 298–300. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309112000474
PMCID:
PMC3310535
Yi-Ting Liao, Ko-Hsin Chin, Wei-Ting Kuo, Mary Lay-Cheng Chuah, Zhao-Xun Liang, Shan-Ho Chou

Crystals of the XccFimXEAL–c-di-GMP and SeMet-XccFimXEAL–c-di-GMP–XccPilZ complexes from X. campestris diffracted to resolutions of 2.5 and 2.7 Å, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 301–305. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309112000590
PMCID:
PMC3310536
Marcelo Vizoná Liberato, Wesley Cardoso Generoso, Wilson Malagó, Jr, Flávio Henrique-Silva, Igor Polikarpov

T. harzianum endoglucanase III was expressed in P. pastoris, purified and crystalized. A native X-ray diffraction data set was collected.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 306–309. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112000838
PMCID:
PMC3310537
Hideyuki Matsunami, Fadel A. Samatey, Shigehiro Nagashima, Katsumi Imada, Keiichi Namba

Salmonella FlgA, a periplasmic chaperone essential for flagellar P-ring assembly, has been expressed and purified and the crystals have been characterized by X-ray diffraction.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 310–313. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112001327
PMCID:
PMC3310538
Fung T. Lay, Prem K. Veneer, Mark D. Hulett, Marc Kvansakul

TPP3 is a class II plant defensin from tomato. Here, the expression, purification, crystallization and preliminary X-ray crystallographic analysis of recombinant TPP3 are reported in order to define its structure and function in relation to other class II plant defensins.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 314–316. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309112001510
PMCID:
PMC3310539
Yukie Maruyama, Takafumi Itoh, Yu Nishitani, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata

Alginate importer from Sphingomonas sp. A1 is a member of the ABC transporter superfamily that directly transports alginate polysaccharide into the cytoplasm. Crystals of alginate importer in complex with the periplasmic binding protein AlgQ2 diffracted X-rays to 3.3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 317–320. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112001893
PMCID:
PMC3310540
Denise Cavalcante Hissa, Gustavo Arruda Bezerra, Britta Obrist, Ruth Birner-Grünberger, Vânia Maria Maciel Melo, Karl Gruber

The purification, crystallization and MS analysis of a natural surfactant protein from the frog L. vastus are described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 321–323. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112002679
PMCID:
PMC3310541
Kanako Shinmura, Norifumi Muraki, Ayako Yoshida, Toshiharu Hase, Genji Kurisu

Cocrystallization of ferredoxin and glutamate synthase was successfully carried out and the resulting crystals were demonstrated to be suitable for structural determination of an electron-transfer complex of these two proteins.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 324–327. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112003387
PMCID:
PMC3310542
Misumi Kataoka, Han-Woo Kim, Kazuhiko Ishikawa

A hyperthermostable endoglucanase from P. furiosus expressed as a truncated form without the N-terminal amino acids was crystallized. The crystal diffracted to a resolution of 1.07 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 328–329. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112003740
PMCID:
PMC3310543
Takenori Satomura, Akihiro Hiraki, Tomoyuki Kawai, Ryushi Kawakami, Toshihisa Ohshima, Haruhiko Sakuraba

A galactose 1-phosphate uridylyltransferase from a hyperthermophilic archaeon was successfully isolated and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 330–332. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112003880
PMCID:
PMC3310544
Sam Griffiths, Robert T. Byrne, Alfred A. Antson, Fiona Whelan

The catalytic domain of human Dus2-like enzyme was purified and crystallized, and data were collected to 1.9 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 333–336. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309112003831
PMCID:
PMC3310545
Maria João Marques-Carvalho, João Henrique Morais-Cabral

The crystallization conditions and preliminary crystal characterization of the cytoplasmic cyclic nucleotide-binding homology domain from the mouse EAG potassium channel are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 337–339. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112004216
PMCID:
PMC3310546
Jian Gao, Yuanyuan Ma, Yuna Sun, Huadong Zhao, Dapeng Hong, Liming Yan, Zhiyong Lou

The cytoplasmic domain of BRI1-associated kinase 1 from A. thaliana has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.6 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 340–342. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309112004605
PMCID:
PMC3310547
Jun-ichi Wachino, Yoshihiro Yamaguchi, Shigetarou Mori, Yuriko Yamagata, Yoshichika Arakawa, Keigo Shibayama

The subclass B3 metallo-β-lactamase SMB-1 was crystallized by the hanging-drop vapour-diffusion method. Two types of crystals belonging to space group P31 were obtained.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 343–346. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112004691
PMCID:
PMC3310548
Abd Ghani Abd Aziz, Sergey N. Ruzheinikov, Svetlana E. Sedelnikova, Rahmah Mohamed, Sheila Nathan, Patrick J. Baker, David W. Rice

The L1 ribosomal protein from B. pseudomallei has been overexpressed, purified and crystallized in a form suitable for X-ray analysis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 347–350. Anglais. Published online 2012 February 22. doi: 10.1107/S1744309112004800
PMCID:
PMC3310549
Stephanie Glaser, Matthew K. Higgins

The crystallization of Tic22 from P. falciparum is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 351–354. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112004952
PMCID:
PMC3310550
Jing Chen, Hiroyuki Morita, Ryohei Kato, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe

Residues 38–440 of CdpNPT from A. fumigatus were overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 2.5 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 355–358. Anglais. Published online 2012 February 23. doi: 10.1107/S1744309112004964
PMCID:
PMC3310551
Radu Huculeci, Lieven Buts, Tom Lenaerts, Nico A. J. van Nuland, Abel Garcia-Pino

The SH2 domain of the human protein tyrosine kinase Fyn has been expressed, purified and crystallized in the unbound state and in complex with a high-affinity phosphotyrosine peptide.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 March 1; 68(Pt 3): 359–364. Anglais. Published online 2012 February 29. doi: 10.1107/S1744309112004186
PMCID:
PMC3310552

Les articles de Acta Crystallographica Section F: Structural Biology and Crystallization Communications ont été offerts à titre gracieux par International Union of Crystallography.