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Volume 67(Pt 4);  2011 April 1

Structural Communications

Andrea Schmidt, Martha Teeter, Edgar Weckert, Victor S. Lamzin

A protein crystallographic data collection using the PETRA II source sets the record in crystallographic resolution for a biological macromolecule (0.48 Å) for the small protein crambin and increases available data for refinement by a factor of 1.5. The quality of the data collected is evident by comparing the merging R factor between two different crambin data sets, collected from

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Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 424–428. Published online 2011 March 24. doi: 10.1107/S1744309110052607
PMCID:
PMC3080141
D. Wade Abbott, Alisdair Boraston

EndoD from S. pneumoniae is a large multimodular virulence factor that contains a putative carbohydrate-binding module (CBM). Here, the structure of the putative CBM is described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 429–433. Published online 2011 March 24. doi: 10.1107/S1744309111001874
PMCID:
PMC3080142
Oluwatoyin Ajibola Asojo, Oluyomi Adebola Asojo, Michelle N. Ngamelue, Kohei Homma, Oksana Lockridge

The cocrystallization of recombinant full-length human BChE with cocaine yields a 3.0 Å resolution structure of the complex with the hydrolysis product benzoic acid. The benzoate is positioned in the acyl cavity, where it appears to forms a hydrogen bond to the active-site Ser.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 434–437. Published online 2011 March 24. doi: 10.1107/S1744309111004805
PMCID:
PMC3080143
Andrew C. Kruse, Medora J. Huseby, Ke Shi, Jeff Digre, Douglas H. Ohlendorf, Cathleen A. Earhart

The structure of a mutant β toxin from S. aureus in which a hydrophobic β-hairpin has been deleted shows unanticipated domain swapping leading to the formation of conformationally flexible dimers.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 438–441. Published online 2011 March 24. doi: 10.1107/S1744309111005239
PMCID:
PMC3080144
Vadim Rimsa, Thomas Eadsforth, William N. Hunter

Crystallographic analysis of the human SENP1 catalytic domain identified a well ordered Co2+ ion that contributes to intermolecular interactions relevant to crystallization of the enzyme. The presence of this ion was overlooked in previous studies.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 442–445. Published online 2011 March 24. doi: 10.1107/S1744309111005835
PMCID:
PMC3080145
Teresa E. Clarke, Vladimir Romanov, Robert Lam, Scott A. Gothe, Srinivasa R. Peddi, Ekaterina B. Razumova, Richard S. A. Lipman, Arthur A. Branstrom, Nickolay Y. Chirgadze

The structure of peptidyl-tRNA hydrolase from F. tularensis, the causative agent of tularemia, is comparable to that of other bacterial peptidyl-tRNA hydrolases, with most residues in the active site conserved amongst the family. The resultant reagents, structural data, and analyses provide essential information for structure-based design of novel inhibitors for this class of proteins.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 446–449. Published online 2011 March 26. doi: 10.1107/S174430911100515X
PMCID:
PMC3080146

Crystallization Communications

Keigo Inoue, Nobutada Tanaka, Arayo Haga, Kyohei Yamasaki, Tomonobu Umeda, Yoshio Kusakabe, Yasumitsu Sakamoto, Takamasa Nonaka, Yoshihiro Deyashiki, Kazuo T. Nakamura

The α isoform of human autotaxin has been crystallized. Diffraction data were collected to 3.0 Å resolution using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 450–453. Published online 2011 March 24. doi: 10.1107/S174430911005311X
PMCID:
PMC3080147
Yi Yang, James A. Garnett, Stephen Matthews

The Enteroaggregative Escherichia coli AAF/II pilus plays an important role in the attachment to and invasion of the host during the initial stages of colonization. Here, the major adhesive subunit of AAF/II has been crystallized at pH 3.4 and diffraction data have been collected to 2.1 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 454–456. Published online 2011 March 25. doi: 10.1107/S1744309111001412
PMCID:
PMC3080148
Ok Kyeung Park, Hyun Ho Park

In this study, the CIDE domains of Drep2 and Drep3 from Drosophila melanogaster were purified in Escherichia coli, after which they formed a stable complex in vitro and were crystallized by the hanging-drop vapour-diffusion method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 457–460. Published online 2011 March 25. doi: 10.1107/S1744309111003113
PMCID:
PMC3080149
Kenneth P. Holbourn, Matthew D. Lloyd, Andrew S. Thompson, Michael D. Threadgill, K. Ravi Acharya

The human histone deacetylase sirtuin 1 was expressed and purified. Crystals were obtained and diffracted to 3.45 Å in space group P622.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 461–463. Published online 2011 March 25. doi: 10.1107/S1744309111003277
PMCID:
PMC3080150
Fiona Cuskin, Alexandra S. Solovyova, Richard J. Lewis, Paul R. Race

The expression, purification and crystallization of the trans-acting acyltransferase PksC from the bacillaene hybrid polyketide synthase/nonribosomal peptide synthetase is described. The crystals belonged to the orthorhombic space group P212121 and diffracted to 1.44 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 464–466. Published online 2011 March 25. doi: 10.1107/S1744309111003484
PMCID:
PMC3080151
Paula S. Salgado, Robert Yan, Fiona Rowan, Ernesto Cota

Details of the expression and crystallization of the N-terminal fragment of Als9-2, an adhesin from the human commensal/pathogenic fungus C. albicans, are reported. Preliminary analysis of the collected X-ray data is also discussed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 467–470. Published online 2011 March 25. doi: 10.1107/S1744309111003460
PMCID:
PMC3080152
Sudipta Bhattacharyya, Debajyoti Dutta, Ananta Kumar Ghosh, Amit Kumar Das

The cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of an inositol monophosphatase family protein (SAS2203) from S. aureus MSSA476 is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 471–474. Published online 2011 March 25. doi: 10.1107/S1744309111003496
PMCID:
PMC3080153
Raquel Melo Alves, Patricia Rosa Feliciano, Suely Vilela Sampaio, Maria Cristina Nonato

l-Amino-acid oxidase from B. atrox has been crystallized by combining seeding with oil modulation of vapour equilibration. A complete data set has been collected to 2.3 Å resolution from a native crystal.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 475–478. Published online 2011 March 25. doi: 10.1107/S1744309111003770
PMCID:
PMC3080154
Julia Drebes, Markus Perbandt, Carsten Wrenger, Christian Betzel

5-(Hydroxyethyl)-4-methylthiazole kinase from S. aureus, which is essential to vitamin B1 metabolism, has been crystallized in space group P1. The crystals diffracted to 2.1 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 479–481. Published online 2011 March 25. doi: 10.1107/S1744309111004192
PMCID:
PMC3080155
Pei-Long Sun, Shu-Xia Lv, Jian-Hua Zhou, Xin-Qi Liu

The equine infectious anaemia virus gp45 ectodomain was cloned, expressed and crystallized. Preliminary crystallographic analysis showed that the protein belonged to space group P63 and contained one molecule per asymmetric unit.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 482–485. Published online 2011 March 25. doi: 10.1107/S174430911100460X
PMCID:
PMC3080156
Ankit Gupta, Clara L. Kielkopf

This work describes the expression, purification, circular dichroism spectrum and crystallization of a central domain from human Splicing Factor 1. A native, 2.5 Å resolution data set is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 486–490. Published online 2011 March 25. doi: 10.1107/S1744309111004623
PMCID:
PMC3080157
Ana S. Luís, Victor D. Alves, Maria J. Romão, José A. M. Prates, Carlos M. G. A. Fontes, Shabir Najmudin

The novel N-terminal carbohydrate-binding module of the family 5 glycoside hydrolase endoglucanase Cel5A from E. cellulosolvens has been crystallized and MAD data have been collected to 1.75 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 491–493. Published online 2011 March 25. doi: 10.1107/S1744309111004246
PMCID:
PMC3080158
Nobuo Okazaki, Takao Arimori, Masami Nakazawa, Kazutaka Miyatake, Mitsuhiro Ueda, Taro Tamada

The catalytic domain of a novel chitinase, which is a member of GH family 23, from the moderately thermophilic bacterium Ralstonia sp. A-471 was crystallized and diffraction data were collected to 1.85 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 494–497. Published online 2011 March 26. doi: 10.1107/S1744309111004751
PMCID:
PMC3080159
Ondřej Vaněk, Jiří Brynda, Kateřina Hofbauerová, Zdeněk Kukačka, Petr Pachl, Karel Bezouška, Pavlína Řezáčová

The fungal β-N-acetylhexosaminidase from A. oryzae was crystallized and diffraction data were collected from two crystal forms to 3.2 and 2.4 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 498–503. Published online 2011 March 26. doi: 10.1107/S1744309111004945
PMCID:
PMC3080160
SangYoun Park, Brian R. Crane

CheW from T. maritima has been crystallized (space group P63, unit-cell parameters a = b = 61.265, c = 361.045 Å). Diffraction data have been collected to 3.1 Å resolution using synchrotron X-ray radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 504–506. Published online 2011 March 30. doi: 10.1107/S1744309111005951
PMCID:
PMC3080161
Joane Kathelen Rustiguel, Matheus Pinto Pinheiro, Ana Paula Ulian Araújo, Maria Cristina Nonato

Recombinant chlorocatechol 1,2-dioxygenase from P. putida has been crystallized in the presence of polyethylene glycol as the precipitant agent and magnesium acetate as an additive. The structure was solved by the MR-SAD approach.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 507–509. Published online 2011 March 30. doi: 10.1107/S174430911100635X
PMCID:
PMC3080162
Tae-ho Jang, Jin Hee Park, Ju-Hong Jeon, Dong-Sup Lee, Kihang Choi, In-Gyu Kim, Young Whan Kim, Hyun Ho Park

The N-terminal domain of the human ribosomal protein L7a (RPL7a) was crystallized. The crystals were found to belong to the tetragonal space group P4122 or P4322, with unit-cell parameters a = 92.28, b = 92.28, c = 236.59 Å. The crystals were obtained at 293 K and diffracted to a resolution of 3.5 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 510–512. Published online 2011 March 30. doi: 10.1107/S1744309111006415
PMCID:
PMC3080163
Jin Myung Choi, Hee Gon Kim, Jeong-Sun Kim, Hyung-Seop Youn, Soo Hyun Eom, Sung-Lim Yu, Si Wouk Kim, Sung Haeng Lee

In order to obtain molecular insights into the methanol-oxidizing system of M. aminisulfidivorans, a native heterotetrameric α2β2 methanol dehydrogenase complex was directly purified from M. aminisulfidivorans MPT grown in the presence of methanol and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 513–516. Published online 2011 March 30. doi: 10.1107/S1744309111006713
PMCID:
PMC3080164
Christin Römer, Silke I. Patzer, Reinhard Albrecht, Kornelius Zeth, Volkmar Braun

The colicin M immunity protein Cmi protects E. coli cells against killing by colicin M. The Cmi protein was produced for structure determination and crystals were obtained which diffracted to high resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 517–520. Published online 2011 March 30. doi: 10.1107/S1744309111006737
PMCID:
PMC3080165
Zheng Fan, Jianxun Qi, Yawei Shi, Yiwei Liu

A recombinant cyclic imide hydrolase from P. putida YZ-26 has been crystallized by the hanging-drop method. This will be helpful in understanding the role of CIH in pyrimidine metabolism and organic acid bioconversion.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 521–523. Published online 2011 March 30. doi: 10.1107/S1744309111006725
PMCID:
PMC3080166
Joseph Abhilash, K. Geethanandan, S. R. Bharath, C. Sadasivan, M. Haridas

A galactose specific lectin was purified from the seeds of a tropical tree, Butea monosperma. Its X-ray structure was solved by molecular replacement.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 April 1; 67(Pt 4): 524–526. Published online 2011 March 30. doi: 10.1107/S1744309111006853
PMCID:
PMC3080167

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography