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Volume 67(Pt 11);  2011 November 1

Editorial

Howard M. Einspahr, Manfred S. Weiss
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1309. Published online 2011 October 29. doi: 10.1107/S1744309111044277
PMCID:
PMC3212441

Structural Communications

James E. Tooley, Victor Khangulov, Jonathan P. B. Lees, Jamie L. Schlessman, Maria C. Bewley, Annie Heroux, Jürgen Bosch, R. Blake Hill

A 1.75 Å resolution crystal structure of the Fis1 cytoplasmic domain from Saccharomyces cerevisiae is reported which adopts a tetratricopeptide-repeat fold.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1310–1315. Published online 2011 October 25. doi: 10.1107/S1744309111029368
PMCID:
PMC3212442
Brahm J. Yachnin, Damien Y. Colin, Jordan P. Volpato, Maximilian Ebert, Joelle N. Pelletier, Albert M. Berghuis

A modification of previously published protocols for the crystallization of homotetrameric R67 DHFR was used to crystallize a mixed wild-type and variant tandem dimer of R67 DHFR. Surprisingly, a fully wild-type crystal structure was obtained, apparently as a consequence of selective proteolytic degradation of the variant.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1316–1322. Published online 2011 October 25. doi: 10.1107/S1744309111030417
PMCID:
PMC3212443
Farhad Forouhar, Nabila Saadat, Munif Hussain, Jayaraman Seetharaman, Insun Lee, Haleema Janjua, Rong Xiao, Ritu Shastry, Thomas B. Acton, Gaetano T. Montelione, Liang Tong

Crystal structures of the putative ATP pyrophosphatase PF0828 reveal a new domain (EGT domain), and a large conformational change upon ATP binding.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1323–1327. Published online 2011 October 25. doi: 10.1107/S1744309111031447
PMCID:
PMC3212444
Shuai Chen, Yibei Xiao, Rajesh Ponnusamy, Jinzhi Tan, Jian Lei, Rolf Hilgenfeld

The crystal structure of the SH3 domain of the p85β subunit of human PI3K was determined to 2.0 Å resolution by molecular replacement. The overall structure is very similar to that of the p85α subunit of PI3K. The binding of two proline-rich ligand peptides to p85β SH3 was also characterized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1328–1333. Published online 2011 October 25. doi: 10.1107/S1744309111031691
PMCID:
PMC3212445
Toshiyuki Chatake, Takuya Ishikawa, Yasuhide Yanagisawa, Taro Yamada, Ichiro Tanaka, Satoru Fujiwara, Yukio Morimoro

A high-resolution X-ray crystallographic study of the effects of solvent deuteration on the crystallization of proteinase K shows negligibly small degradations of the crystals owing to solvent deuteration and small structural differences between nondeuterated and deuterated crystals of proteinase K.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1334–1338. Published online 2011 October 25. doi: 10.1107/S1744309111031903
PMCID:
PMC3212446
William J. Cook, Olga Senkovich, Debasish Chattopadhyay

The crystal structure of the catalytic domain of ARF GTPase-activating protein (ARFGAP) of Plasmodium falciparum has been determined at 2.4 Å resolution and compared with the structures of mammalian ARFGAPs.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1339–1344. Published online 2011 October 25. doi: 10.1107/S1744309111032507
PMCID:
PMC3212447
Christopher M. Bianchetti, Nathaniel L. Elsen, Brian G. Fox, George N. Phillips, Jr

The structural characterization of cellobiose phosphorylase from C. thermocellum reveals the residues involved in phosphate coordination and provides insight into substrate binding and discrimination.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1345–1349. Published online 2011 October 25. doi: 10.1107/S1744309111032660
PMCID:
PMC3212448
Kelly H. Kim, Suraaj Aulakh, Wendy Tan, Mark Paetzel

The assembly of bacterial outer membrane proteins is catalyzed by the BAM complex, which is made up of five proteins (BamA–E). Structural and bioinformatic analysis of the C-terminal domain of E. coli BamC (BamCC) suggests that a negatively charged conserved surface on BamCC may be an important protein–protein interaction site.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1350–1358. Published online 2011 October 25. doi: 10.1107/S174430911103363X
PMCID:
PMC3212449

Crystallization Communications

Veerendra Kumar, Nagarajan Mallika, J. Sivaraman

The expression, purification, crystallization and diffraction of two methyltransferases BT_2972 and BVU_3255 from two Bacteroides species of antibiotic-resistant pathogens from the human intestine are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1359–1362. Published online 2011 October 27. doi: 10.1107/S1744309111031812
PMCID:
PMC3212450
Miha Pavšič, Brigita Lenarčič

The extracellular part of the human epithelial cell-adhesion molecule (EpCAM; CD326) was cloned, mutated to prevent N-linked glycosylation, expressed in insect cells, purified and crystallized. Diffraction data were collected to 1.95 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1363–1366. Published online 2011 October 27. doi: 10.1107/S1744309111031897
PMCID:
PMC3212451
Yuka Echizen, Tomoya Tsukazaki, Naoshi Dohmae, Ryuichiro Ishitani, Osamu Nureki

SecDF is a multi-path membrane protein required for efficient protein translocation and integration via the Sec translocon. Crystals of the first periplasmic domain of SecDF, an essential element for SecDF function, diffracted X-rays to 2.3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1367–1370. Published online 2011 October 27. doi: 10.1107/S1744309111031885
PMCID:
PMC3212452
Karina U. Sturm, Martin H. Griessl, Carolin Wagner, Jörg Deiwick, Michael Hensel, Yves A. Muller

SiiE from S. enterica is a giant adhesion molecule with a total of 5559 amino acids that is specifically required for initial contact between the pathogen and polarized epithelial cells. Using limited proteolysis, a fragment of SiiE that encompasses Ig domains 50–52 was produced and crystallized and diffraction data were collected to 1.85 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1371–1374. Published online 2011 October 27. doi: 10.1107/S1744309111032039
PMCID:
PMC3212453
Xiaoting Qiu, Kai Huang, Yiwei Liu, Xiao Zhang, Yongxiang Gao

This paper reports the cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of the first C2 domain of synaptotagmin 5.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1375–1377. Published online 2011 October 27. doi: 10.1107/S1744309111032155
PMCID:
PMC3212454
Ivana Leščić Ašler, Jasenka Pigac, Dušica Vujaklija, Marija Luić, Zoran Štefanić

A lipolytic enzyme of the SGNH-hydrolase family from S. rimosus was inhibited with 3,4-dichloroisocoumarin, crystallized and analyzed using X-ray diffraction.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1378–1381. Published online 2011 October 27. doi: 10.1107/S1744309111032222
PMCID:
PMC3212455
Samir Das, Sanjay Dey, Trina Roy, Udayaditya Sen

A heat-shock protein from V. cholerae (VcHsp31) has been cloned, expressed, purified and crystallized. Crystals of VcHsp31 belonged to a monoclinic space group and diffracted to 1.9 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1382–1385. Published online 2011 October 27. doi: 10.1107/S1744309111032970
PMCID:
PMC3212456
Michael D. W. Griffin, Jagan M. Billakanti, Juliet A. Gerrard, Renwick C. J. Dobson, F. Grant Pearce

Dihydrodipicolinate synthase (DHDPS) catalyzes the first committed step of the lysine-biosynthetic pathway in plants and bacteria. The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis (to 2.5 Å resolution) of DHDPS2 from A. thaliana are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1386–1390. Published online 2011 October 27. doi: 10.1107/S1744309111033276
PMCID:
PMC3212457
Xingyou Ye, Ping Xiao, Xiaowei Hu, Yunyun Chen, Liping Zhang, Wei Xie, Xiaopeng Hu

The recombinant RPB5 subunit of human RNA polymerase II has been crystallized by vapour diffusion in hanging drops.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1391–1393. Published online 2011 October 27. doi: 10.1107/S1744309111033288
PMCID:
PMC3212458
Megha Aggarwal, Sonali Dhindwal, Shivendra Pratap, Richard J. Kuhn, Pravindra Kumar, Shailly Tomar

A 17 kDa capsid protease domain from Aura virus was purified, crystallized together with its complex with dioxane and characterized by the X-ray diffraction method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1394–1398. Published online 2011 October 27. doi: 10.1107/S174430911103404X
PMCID:
PMC3212459
Sara N. Andres, Murray S. Junop

A complex of the human DNA-repair proteins XRCC4 and XLF was crystallized under high-salt and extreme dehydration conditions to produce diffraction to 3.9 Å resolution. Initial phasing information was obtained from molecular replacement with single-wavelength anomalous diffraction using tantalum bromide clusters.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1399–1402. Published online 2011 October 27. doi: 10.1107/S1744309111033549
PMCID:
PMC3212460
Jinsue Song, Hyerim Hong, Saehae Choi, Yong Hee Lee, Eiki Yamashita, Suk-Chul Bae, Il Yeong Park, Soo Jae Lee

The overproduction, purification, crystallization and preliminary X-ray analysis of the MST2 SARAH domain are described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1403–1405. Published online 2011 October 27. doi: 10.1107/S1744309111033823
PMCID:
PMC3212461
Guido Hansen, Britta Schwarzloh, Annika Rennenberg, Volker T. Heussler, Rolf Hilgenfeld

The macromolecular complex of ICP (inhibitor of cysteine proteases) from P. berghei and falcipain-2 from P. falciparum has been prepared and crystallized, and a diffraction data set has been collected to a resolution of 2.6 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1406–1410. Published online 2011 October 27. doi: 10.1107/S1744309111034592
PMCID:
PMC3212462
Ibolya Leveles, Gergely Róna, Imre Zagyva, Ábris Bendes, Veronika Harmat, Beáta G. Vértessy

The cloning, purification, crystallization and preliminary X-ray diffraction analysis of a novel staphylococcal phage dUTPase is reported. This protein contains a specific polypeptide insertion that is potentially responsible for modulation of expression of superantigenicity island genes.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1411–1413. Published online 2011 October 27. doi: 10.1107/S1744309111034580
PMCID:
PMC3212463
Takuo Osawa, Hideko Inanaga, Satoshi Kimura, Naohiro Terasaka, Tsutomu Suzuki, Tomoyuki Numata

A. fulgidus TiaS was cocrystallized with tRNAIle2 and ATP and X-ray diffraction data were collected to 2.9 Å resolution using a synchrotron-radiation source.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1414–1416. Published online 2011 October 27. doi: 10.1107/S1744309111034890
PMCID:
PMC3212464
Lennart Wirthmueller, Jonathan D. Jones, Mark J. Banfield

RXLR3 is an oomycete effector from H. arabidopsidis. Crystals of the RXLR3 effector domain were obtained and X-ray data were recorded to a resolution of 1.8 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1417–1420. Published online 2011 October 27. doi: 10.1107/S1744309111035901
PMCID:
PMC3212465
Ju Young Bae, Hyun Ho Park

The PYD domain of human NALP3 was crystallized. The crystals were found to belong to the primitive monoclinic space group P21, with unit-cell parameters a = 42.03, b = 60.14, c = 51.61 Å, β = 107.40°. The crystals were obtained at 293 K and diffracted to a resolution of 1.7 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1421–1424. Published online 2011 October 27. doi: 10.1107/S1744309111035937
PMCID:
PMC3212466
Takenori Shibahara, Takenori Satomura, Ryushi Kawakami, Toshihisa Ohshima, Haruhiko Sakuraba

A dye-linked d-lactate dehydrogenase from a hyperthermophilic archaeon was successfully isolated and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1425–1427. Published online 2011 October 27. doi: 10.1107/S1744309111036098
PMCID:
PMC3212467
Melina Haupt, Matthew P. Blakeley, Susana C. M. Teixeira, Sax A. Mason, Edward P. Mitchell, Jonathan B. Cooper, V. Trevor Forsyth

Human transthyretin, a hormone-binding protein of high abundance in blood and cerebrospinal fluid, is intrinsically amyloidogenic. Preliminary results from a neutron diffraction analysis of this protein that may shed light on the mechanism of tetramer dissociation and successive fatal aggregation are presented.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1428–1431. Published online 2011 October 27. doi: 10.1107/S1744309111036244
PMCID:
PMC3212468
Marcus Fislage, Martine Roovers, Stefan Münnich, Louis Droogmans, Wim Versées

Two orthologous putative tRNA methyltransferases from P. furiosus and T. thermophilus have been expressed, purified and crystallized. X-ray diffraction data were collected to 2.2 and 2.05 Å, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1432–1435. Published online 2011 October 27. doi: 10.1107/S1744309111036347
PMCID:
PMC3212469
Yanaisis Álvarez, María Esteban-Torres, Iván Acebrón, Blanca de las Rivas, Rosario Muñoz, Martín Martínez-Ripoll, José M. Mancheño

The Q88Y25_Lacpl esterase from L. plantarum WCFS1 has been recombinantly expressed, purified and crystallized. A native diffraction data set has been collected to 2.24 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1436–1439. Published online 2011 October 27. doi: 10.1107/S1744309111036682
PMCID:
PMC3212470
Fukun Chen, Kui Lin, Zhenyi Zhang, Leyi Chen, Xiaoshan Shi, Chunyang Cao, Zhijun Wang, Jingdan Liang, Zixin Deng, Geng Wu

DndE is one of five essential proteins that are required for the DNA phosphorothioation process. However, its exact biochemical role in sulfur modification of DNA remains unclear. In this study, the DndE protein homologue from Salmonella enterica serovar Cerro 87 was overexpressed, purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1440–1442. Published online 2011 October 27. doi: 10.1107/S1744309111036694
PMCID:
PMC3212471
Jinxiu Zhang, Xue Yang, Yuequan Shen, Jiafu Long

Four different L27PATJ–(L27N,L27C)Pals1–L27MALS constructs have been cloned, expressed, purified and crystallized. Crystals of tripartite complex 1 of L27PATJ–(L27N,L27C)Pals1–L27MALS diffracted to 2.05 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1443–1447. Published online 2011 October 27. doi: 10.1107/S174430911103689X
PMCID:
PMC3212472
Xiaoting Qiu, Kai Huang, Jinming Ma, Yongxiang Gao

The expression, purification, crystallization and X-ray diffraction analysis of tRNA m1A58 methyltransferase from S. cerevisiae are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1448–1450. Published online 2011 October 27. doi: 10.1107/S174430911103733X
PMCID:
PMC3212473
Mohammad Syed Ahangar, Yogesh Khandokar, Nazia Nasir, Rajan Vyas, Bichitra K. Biswal

HisB from M. tuberculosis was cloned, overexpressed in M. smegmatis, purified and crystallized in three crystal forms.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1451–1456. Published online 2011 October 27. doi: 10.1107/S1744309111037201
PMCID:
PMC3212474
Cecilia Ortíz, Nicole Larrieux, Andrea Medeiros, Horacio Botti, Marcelo Comini, Alejandro Buschiazzo

The crystallization of glucose-6-phosphate dehydrogenase from the human pathogen T. cruzi is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1457–1461. Published online 2011 October 27. doi: 10.1107/S1744309111037821
PMCID:
PMC3212475
Alessia Ruggiero, Jonathan D. Dattelbaum, Anna Pennacchio, Luisa Iozzino, Maria Staiano, Matthew S. Luchansky, Bryan S. Der, Rita Berisio, Sabato D’Auria, Luigi Vitagliano

In this study, the hyperthermostable arginine-binding protein isolated from T. maritima has been crystallized in ligand-free and arginine-bound forms. Crystals of the apo and holo forms diffracted to 2.2 and 2.7 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 November 1; 67(Pt 11): 1462–1465. Published online 2011 October 27. doi: 10.1107/S1744309111037341
PMCID:
PMC3212476

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography