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Volume 67(Pt 10);  2011 October 1

Editorial

Manfred S. Weiss, Howard Einspahr
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1159. Published online 2011 September 30. doi: 10.1107/S1744309111039492
PMCID:
PMC3212352

Structural Communications

Victoria L. Green, Anil Verma, Raymond J. Owens, Simon E. V. Phillips, Stephen B. Carr

Metallo-β-lactamase enzymes confer resistance to a wide variety of commonly used antibiotics; this resistance poses a significant threat to human health where it arises in a pathogenic bacterium. Here, the structure of the novel metallo-β-lactamase NDM-1, which is capable of providing resistance against a broad spectrum of antibacterial agents, has been solved.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1160–1164. Published online 2011 September 6. doi: 10.1107/S1744309111029654
PMCID:
PMC3212353
Alexey Teplyakov, Galina Obmolova, Thomas Malia, Gary Gilliland

Comparison of the Fab structure in the free and antigen-bound form indicates an induced-fit mechanism of IL-13 recognition by antibody C836 through rigid-body rotation of the VL and VH domains.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1165–1167. Published online 2011 September 24. doi: 10.1107/S1744309111027746
PMCID:
PMC3212354
Tianjun Sun, Koto Hayakawa, Marie E. Fraser

ATP-citrate lyase catalyzes the production of acetyl-CoA from citrate, CoA and ATP. Crystals were grown of the amino-terminal portion of the human enzyme in the presence of tartrate, ATP and magnesium ions. The crystal structure shows the inhibitor tartrate and the product ADP–Mg2+ bound to the protein.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1168–1172. Published online 2011 September 24. doi: 10.1107/S1744309111028363
PMCID:
PMC3212355
Yan Kang, Yong-Xing He, Meng-Xi Zhao, Wei-Fang Li

The crystal structures of both native manganese SOD2 and iron-substituted SOD2 from S. cerevisiae were solved. The surface-potential properties showed a similar pattern to those of Cu/Zn-SODs.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1173–1178. Published online 2011 September 24. doi: 10.1107/S1744309111029186
PMCID:
PMC3212356
K. El Omari, B. Dhaliwal, J. Ren, N. G. A. Abrescia, M. Lockyer, K. L. Powell, A. R. Hawkins, D. K. Stammers

Structures of the nucleocapsid protein of human respiratory syncytial virus have been obtained from two crystal forms. The packing in one crystal form suggests that ionic interactions contribute to the stability of the native helical form of HRSVN.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1179–1183. Published online 2011 September 24. doi: 10.1107/S1744309111029228
PMCID:
PMC3212357
Jolanta Kopec, Robert Schnell, Gunter Schneider

The crystal structure of recombinant UbiX has been determined to 1.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1184–1188. Published online 2011 September 24. doi: 10.1107/S174430911102923X
PMCID:
PMC3212358
Chih-Wen Tseng, Tzu-Ping Ko, Rey-Ting Guo, Jian-Wen Huang, Hao-Ching Wang, Chun-Hsiang Huang, Ya-Shan Cheng, Andrew H.-J. Wang, Je-Ruei Liu

The structure of a complex of an endoglucanase with cellotriose was determined with a hexagonal unit cell and showed how the substrate interacts with the enzyme.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1189–1194. Published online 2011 September 24. doi: 10.1107/S1744309111032428
PMCID:
PMC3212359

Crystallization Communications

Anurag Misra, Tora Biswas, Sreetama Das, Uttara Marathe, Devinder Sehgal, Rajendra P. Roy, Ramakumar Suryanarayanarao

Sortase A from S. pneumoniae has been crystallized in two crystal forms: diamond-shaped (construct ΔN59SrtA) and rod-shaped (construct ΔN81SrtA). The diamond-shaped crystals diffracted poorly to 4.0 Å resolution and belonged to a tetragonal system, whereas the rod-shaped crystals diffracted to 2.91 Å resolution and belonged to space group P21.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1195–1198. Published online 2011 September 24. doi: 10.1107/S1744309111029952
PMCID:
PMC3212360
Wonchull Kang, Jin Kuk Yang

The hexameric ND1 fragment of p97/VCP was crystallized in complex with the UBX domain of FAF1. X-ray diffraction data were collected to 3.60 Å resolution and the crystals belonged to space group I222.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1199–1202. Published online 2011 September 24. doi: 10.1107/S1744309111031794
PMCID:
PMC3212361
Yogesh Mishra, Michael Hall, Neha Chaurasia, Lal Chand Rai, Stefan Jansson, Wolfgang P. Schröder, Uwe H. Sauer

Alkyl hydroperoxide reductase from Anabaena sp. PCC 7120 was expressed, purified and crystallized. Diffraction data to 2.5 Å resolution revealed that the crystals belonged to space group P212121, with unit-cell parameters a = 80, b = 102, c = 109.6 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1203–1206. Published online 2011 September 24. doi: 10.1107/S1744309111025747
PMCID:
PMC3212362
Karina Persson

Crystals of FimP from A. oris were obtained. To facilitate selenomethionine labelling, three methionines were introduced by site-directed mutagenesis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1207–1210. Published online 2011 September 24. doi: 10.1107/S1744309111025929
PMCID:
PMC3212363
Pravas Kumar Baral, Barbara Wieland, Mridula Swayampakula, Magdalini Polymenidou, Adriano Aguzzi, Nat N. V. Kav, Michael N. G. James

The complex of MoPrP(120–232) and Fab POM1 has been crystallized (space group C2, unit-cell parameters a = 83.68, b = 106.9, c = 76.25 Å, β = 95.6°). Diffraction data to 2.30 Å resolution have been collected using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1211–1213. Published online 2011 September 24. doi: 10.1107/S1744309111026273
PMCID:
PMC3212364
Barbara Addario, Shenghua Huang, Uwe H. Sauer, Lars Backman

The rod domain of α-actinin-2 from E. histolytica, the aetiological agent of human amoebiasis, was crystallized and synchrotron diffraction data were collected from crystals vitrified in liquid nitrogen.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1214–1217. Published online 2011 September 24. doi: 10.1107/S1744309111026066
PMCID:
PMC3212365
Baisong Zheng, Wen Yang, Yuguo Wang, Zhiyong Lou, Yan Feng

In order to confirm the effect on cocrystallization, two N-terminally truncated variants of a thermostable endoglucanase from the thermophilic bacterium Fervidobacterium nodosum Rt17-B1 were constructed, purified and cocrystallized at 291 K.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1218–1220. Published online 2011 September 29. doi: 10.1107/S1744309111033550
PMCID:
PMC3212366
Buabarn Kuaprasert, Kun Silprasit, Natharinee Horata, Pongsak Khunrae, Ratree Wongpanya, Nonlawat Boonyalai, Apichart Vanavichit, Kiattawee Choowongkomon

Crystals of betaine aldehyde dehydrogenase 2 from rice (O. sativa L.) belonged to a C-centred orthorhombic space group and diffraceted X-rays to 2.6 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1221–1223. Published online 2011 September 29. doi: 10.1107/S1744309111030971
PMCID:
PMC3212367
Damià Garriga, Laia Vives-Adrián, Mònica Buxaderas, Frederico Ferreira-da-Silva, Bruno Almeida, Sandra Macedo-Ribeiro, Pedro José Barbosa Pereira, Núria Verdaguer

The 2AB protein derived from the nonstructural P2 region of hepatitis A virus has been cloned, purified and crystallized. The preliminary characterization of native and selenomethionine-derivative crystals is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1224–1227. Published online 2011 September 29. doi: 10.1107/S1744309111026261
PMCID:
PMC3212368
Rui Zhang, Jinyong Zhang, Honglei Ding, Dongshui Lu, Yonglin Hu, Da-Cheng Wang, Quanming Zou

ChuZ–haemin complex crystals belonged to space group C2221, with unit-cell parameters a = 106.474, b = 106.698, c = 52.464 Å, α = β = γ = 90°. Diffraction data have been collected to 2.4 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1228–1230. Published online 2011 September 29. doi: 10.1107/S1744309111026194
PMCID:
PMC3212369
Daniel M. Passon, Mihwa Lee, Archa H. Fox, Charles S. Bond

A truncated heterodimer of human PSPC1–NONO protein, a paraspeckle-specific complex, has been crystallized and the diffraction data collected to a resolution of 1.9 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1231–1234. Published online 2011 September 29. doi: 10.1107/S1744309111026212
PMCID:
PMC3212370
Claudiu M. Nelersa, Brad J. Schmier, Arun Malhotra

The DHH-family phosphoesterase NrnA is involved in the degradation of small RNA oligomers. B. subtilis NrnA crystallized in space group P21 and diffracted X-rays to a resolution of 2.0 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1235–1238. Published online 2011 September 29. doi: 10.1107/S1744309111026509
PMCID:
PMC3212371
Jihui Ren, Gabriel Schaaf, Vytas A. Bankaitis, Eric A. Ortlund, Manish C. Pathak

Yeast Sfh3, a Sec14-like phosphatidylinositol-transfer protein with unique functional properties, has been crystallized. X-ray data sets were collected from native and selenomethionine-substituted crystals to 2.2 and 1.93 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1239–1243. Published online 2011 September 29. doi: 10.1107/S1744309111027096
PMCID:
PMC3212372
Kohsuke Nishimura, Kengo Kitadokoro, Yuki Takegahara, Yo Sugawara, Takuhiro Matsumura, Hajime Karatani, Yukako Fujinaga

The haemagglutinin subcomponent HA3 of the type B botulinum neurotoxin complex, which is important in toxin absorption from the gastrointestinal tract, has been expressed, purified and subsequently crystallized in two crystal forms at different pH values.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1244–1246. Published online 2011 September 29. doi: 10.1107/S1744309111027412
PMCID:
PMC3212373
Jae-Hee Jeong, Ji-Eun Bae, Yeon-Gil Kim

The crystallization of PBP4 from L. monocytogenes is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1247–1249. Published online 2011 September 29. doi: 10.1107/S1744309111027400
PMCID:
PMC3212374
Sang Ho Park, Ho Kyung Chung, Do Jin Kim, Mi Ra Han, Mi Seul Park, Uhtaek Oh, Hyun-Jung Kim, Byung Woo Han

The C-terminal cytosolic domain of mouse anoctamin 1 (mANO1, also known as TMEM16A) was cloned, overexpressed, purified and crystallized. The crystals belonged to space group P212121 and diffracted to 2.3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1250–1252. Published online 2011 September 29. doi: 10.1107/S1744309111027989
PMCID:
PMC3212375
Husam M. A. B. Alsarraf, Fabrice Laroche, Herman Spaink, Søren Thirup, Mickael Blaise

The crystallization of the TLDc domain of oxidation resistance protein 2 from zebrafish is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1253–1256. Published online 2011 September 29. doi: 10.1107/S1744309111027990
PMCID:
PMC3212376
Yano M. K. Yoga, Daouda A. K. Traore, Jacqueline A. Wilce, Matthew C. J. Wilce

The successful preparation of a mutant KH domain representing the first KH domain of PCBP1 and its crystallization in complex with a C-rich DNA are reported. This structure is anticipated to provide high-resolution information that will allow better understanding of the basis of cytosine specificity by PCBPs.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1257–1261. Published online 2011 September 30. doi: 10.1107/S1744309111028004
PMCID:
PMC3212377
Scott J. Callahan, Richard D. Morgan, Rinku Jain, Sharon A. Townson, Geoffrey G. Wilson, Richard J. Roberts, Aneel K. Aggarwal

To further understand the structural basis of MmeI (a type IIL restriction enzyme) DNA-binding specificity, the enzyme has been crystallized in complex with its DNA substrate.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1262–1265. Published online 2011 September 30. doi: 10.1107/S1744309111028041
PMCID:
PMC3212378
Craig Don Paul, Daouda A. K. Traore, Emma Byres, Jamie Rossjohn, Rodney J. Devenish, Csaba Kiss, Andrew Bradbury, Matthew C. J. Wilce, Mark Prescott

eCGP123, an extremely stable GFP with photoswitching properties, has been expressed, purified and crystallized. A diffraction data set has been collected at 2.10 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1266–1268. Published online 2011 September 30. doi: 10.1107/S1744309111028156
PMCID:
PMC3212379
Niels van Oosterwijk, Jan Knol, Lubbert Dijkhuizen, Robert van der Geize, Bauke W. Dijkstra

The gene for 3-ketosteroid Δ4-(5α)-dehydrogenase from R. jostii RHA1 was cloned and overexpressed in E. coli and the protein product was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group C2221 and diffraction data were collected to a resolution of 1.6 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1269–1273. Published online 2011 September 30. doi: 10.1107/S1744309111028727
PMCID:
PMC3212380
Aisyah M. Rehan, Ghader Bashiri, Neil G. Paterson, Edward N. Baker, Christopher J. Squire

The C-terminal domain of FbiB, a bifunctional protein that is essential for the biosynthesis of cofactor F420 in M. tuberculosis, has been expressed, purified and crystallized. The crystals diffracted to 2.0 Å resolution and were suitable for structure determination.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1274–1277. Published online 2011 September 30. doi: 10.1107/S1744309111028958
PMCID:
PMC3212381
Alzoray Rojas-Altuve, César Carrasco-López, Víctor M. Hernández-Rocamora, Jesús M. Sanz, Juan A. Hermoso

PaaX, the main regulator of the phenylacetic acid degradation pathway in bacteria, has been crystallized using the sitting-drop vapour-diffusion method. Preliminary X-ray data to 2.3 Å resolution allowed determination of its unit-cell parameters and space group and initiation of its structure determination.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1278–1280. Published online 2011 September 30. doi: 10.1107/S1744309111029873
PMCID:
PMC3212382
Hiromi Yoshida, Misa Teraoka, Akihide Yoshihara, Ken Izumori, Shigehiro Kamitori

Recombinant l-ribose isomerase from Acinetobacter sp. was crystallized. Diffraction data were collected to 2.2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1281–1284. Published online 2011 September 30. doi: 10.1107/S1744309111030351
PMCID:
PMC3212383
Noelia Bernardo-García, Sergio G. Bartual, Marcus Fulde, Simone Bergmann, Juan A. Hermoso

Phosphoglycerate kinase, a two-domain enzyme involved in the glycolytic pathway, has been crystallized. Both the N-terminal domain and a ternary complex of the full-length enzyme with substrates were crystallized by the sitting-drop vapour-diffusion method. Inclusion of the substrates was critical in order to obtain crystals of the full-length protein.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1285–1289. Published online 2011 September 30. doi: 10.1107/S1744309111030922
PMCID:
PMC3212384
Thomas J. Malia, Galina Obmolova, Jinquan Luo, Alexey Teplyakov, Raymond Sweet, Gary L. Gilliland

Methods that led to the successful crystallization of TLR3 ECD in complex with Fab fragments from three noncompeting, neutralizing anti-TLR3 antibodies are presented. The crystallization of this 238 kDa complex was achieved through fine purification of the quaternary complex of TLR3 ECD with three Fab fragments combined with microseed matrix screening and additive screening.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1290–1295. Published online 2011 September 30. doi: 10.1107/S1744309111030983
PMCID:
PMC3212385
Harkewal Singh, Thomas J. Reilly, Michael J. Calcutt, John J. Tanner

Methods for the expression, purification and crystallization of the class C acid phosphatase from M. bovis are reported. This enzyme is atypical in that it is nearly 20 kDa larger than other known class C acid phosphatases.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1296–1299. Published online 2011 September 30. doi: 10.1107/S1744309111031551
PMCID:
PMC3212386

Laboratory Communications

C. Abad-Zapatero, R. Oliete, S. Rodriguez-Puente, J. Pous, L. Martinelli, M. E. Johnson, A. Guasch

Protein crystals typically deteriorate upon soaking in solutions very different from those used to grow them (the ‘mother liquor’). This is particularly true of solutions containing lower salt concentrations and organic solvents (i.e. DMSO, ethanol). Optimization of the relative humidity environment of the crystals can compensate for these effects and allow recovery of diffraction quality.

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 October 1; 67(Pt 10): 1300–1308. Published online 2011 September 29. doi: 10.1107/S174430911103377X
PMCID:
PMC3212387

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography