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Volume 67(Pt 8);  2011 August 1

Structural Communications

Eiki Yamashita, Atsushi Nakagawa, Junichi Takahashi, Kin-ichi Tsunoda, Seiko Yamada, Shigeki Takeda

The C-terminal domain of a bacteriophage P2 tail-spike protein, gpV, was crystallized and its structure was solved at 1.27 Å resolution. The refined model showed a triple β-helix structure and the presence of iron, calcium and chloride ions.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 837–841. Published online 2011 July 13. doi: 10.1107/S1744309111005999
PMCID:
PMC3151111
Rajkumar Ganesan, Stjepan Jelakovic, Peer R. E. Mittl, Amedeo Caflisch, Markus G. Grütter

Structure-based identification and validation by protein X-ray crystallography of halomethyl ketone derivatives that inhibit caspase-3 without an aspartic acid in the P1 position is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 842–850. Published online 2011 July 13. doi: 10.1107/S1744309111018604
PMCID:
PMC3151112
Miroslaw Tarnawski, Szymon Krzywda, Wojciech Bialek, Mariusz Jaskolski, Andrzej Szczepaniak

The crystal structure of the RuBisCO assembly chaperone RbcX from a thermophilic cyanobacterium has been determined at 1.7 Å resolution. The dimeric structure is capable of a hinge movement (probably connected with binding of the RuBisCO large subunit) pivoted on a kink in two long antiparallel α-helices.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 851–857. Published online 2011 July 13. doi: 10.1107/S1744309111018860
PMCID:
PMC3151113
Philip S. Kerry, Elizabeth Long, Margaret A. Taylor, Rupert J. M. Russell

The structure of a monomeric effector domain from influenza A virus NS1 is presented from diffraction data extending to 1.8 Å resolution. Comparison of this and other NS1 effector-domain structures shows conformational changes at a strand–strand packing interface, hinting at a role for β-strand augmentation in NS1 function.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 858–861. Published online 2011 July 13. doi: 10.1107/S1744309111019312
PMCID:
PMC3151114
Kai Huang, Wei Zhao, Yongxiang Gao, Wenqing Wei, Maikun Teng, Liwen Niu

Saxthrombin, a snake-venom thrombin-like enzyme from Gloydius saxatilis, was purified and crystallized to obtain a high-quality crystal, from which data were acquired to 1.43 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 862–865. Published online 2011 July 13. doi: 10.1107/S1744309111022548
PMCID:
PMC3151115
Stefano Benini, Keith Wilson

The crystal structure of the M. tuberculosis soluble inorganic pyrophosphatase Rv3628 determined at pH 7.0 reveals conformational changes compared with the structure at pH 5.0. The conformational changes arise not only as a consequence of the different pH but also of the presence of two potassium ions bound to the protein.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 866–870. Published online 2011 July 26. doi: 10.1107/S1744309111023323
PMCID:
PMC3151116
Richard C. Page, Jeffrey G. Clark, Saurav Misra

The structure of immunoglobulin-like repeat 10 from human filamin A solved at 2.44 Å resolution suggests the potential effects of mutations correlated with otopalatodigital syndrome spectrum disorders.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 871–876. Published online 2011 July 26. doi: 10.1107/S1744309111024249
PMCID:
PMC3151117

Crystallization Communications

Netanel Tzarum, David Engelberg, Oded Livnah

The p38αY323T active mutant crystallized in two different forms representing different structural features. It has been shown that the crystal profile and the resultant structures are biased by the source microseeds.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 877–884. Published online 2011 July 13. doi: 10.1107/S1744309111017970
PMCID:
PMC3151118
Sean A. Dalrymple, Inder Sheoran, Susan G. W. Kaminskyj, David A. R. Sanders

UDP-glucose-4-epimerase from A. nidulans has been crystallized as a complex with the substrate UDP-galactose using the microbatch method under paraffin oil.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 885–887. Published online 2011 July 19. doi: 10.1107/S1744309111020914
PMCID:
PMC3151119
Nianzhi Zhang, Jianxun Qi, Xiaocheng Pan, ZhaoSan Chen, Xin Li, Feng Gao, Chun Xia

Crystals of swine CD8α diffracted to 1.8 Å resolution and belonged to space group P3221, with unit-cell parameters a = 80.97, b = 80.97, c = 95.19 Å; they contained two molecules in the asymmetric unit. The Matthews coefficient and solvent content were calculated to be 3.23 Å3 Da−1 and 61.89%, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 888–891. Published online 2011 July 19. doi: 10.1107/S1744309111020392
PMCID:
PMC3151120
Kengo Yasuhira, Naoki Shibata, Yasuhito Tanaka, Naoya Kumagai, Yusuke Tanaka, Keisuke Nagai, Dai-ichiro Kato, Masahiro Takeo, Seiji Negoro, Yoshiki Higuchi

Crystals of 6-aminohexanoate-oligomer hydrolase have been obtained by the sitting-drop vapour-diffusion method using sodium citrate as a precipitant. Diffraction data for native and K2PtCl4-derivative crystals were collected to resolutions of 2.00 and 2.20 Å, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 892–895. Published online 2011 July 19. doi: 10.1107/S1744309111022858
PMCID:
PMC3151121
Neelagandan Kamariah, Frank Eisenhaber, Sharmila Adhikari, Birgit Eisenhaber, Gerhard Grüber

The PIG-S71–467 subunit of the yeast GPI transamidase complex was cloned, overexpressed, purified and crystallized. The crystals belonged to space group C2 and diffracted to 3.2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 896–899. Published online 2011 July 19. doi: 10.1107/S1744309111024080
PMCID:
PMC3151122
Guilherme H. M. Salvador, Daniela P. Marchi-Salvador, Lucas B. Silveira, Andreimar M. Soares, Marcos R. M. Fontes

BmooPLA2-I, an acidic, catalytic and nontoxic phospholipase A2 from B. moojeni venom that is able to inhibit platelet aggregation and induce a hypotensive effect, has been crystallized. An X-ray diffraction data set was collected to 1.6 Å resolution and a molecular-replacement solution was obtained.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 900–902. Published online 2011 July 19. doi: 10.1107/S174430911102392X
PMCID:
PMC3151123
Gouri Deb, Karen Boeshanes, William K. Idler, Bijan Ahvazi

Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of a 12R-LOX–chaperone complex are reported. The crystals belonged to the space group P21 and diffracted to 4 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 903–906. Published online 2011 July 19. doi: 10.1107/S1744309111021361
PMCID:
PMC3151124
Xue Guo, Youwei Xu, Ping Wang, Ze Li, Yanhui Xu, Huirong Yang

The expression, purification and crystallization of a PHD domain of human JARID1B are described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 907–910. Published online 2011 July 19. doi: 10.1107/S1744309111021981
PMCID:
PMC3151125
Liping Zhang, Kui Wang, Zhongxing Mo, Yuhuan Liu, Xiaopeng Hu

β-Galactosidase from the psychrotrophic and halotolerant Planococcus sp. L4 (BgaP) was crystallized and a complete data set was collected. There are six protein subunits in the asymmetric unit.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 911–913. Published online 2011 July 20. doi: 10.1107/S1744309111022627
PMCID:
PMC3151126
Ken-ichi Miyazono, Nobumitsu Tabei, Kazuya Marushima, Yasuo Ohnishi, Sueharu Horinouchi, Masaru Tanokura

Glucokinase from S. griseus (SgGlkA) was expressed, purified and crystallized using the sitting-drop vapour-diffusion method. A crystal of SgGlkA in complex with glucose diffracted X-rays to 1.84 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 914–916. Published online 2011 July 20. doi: 10.1107/S1744309111022275
PMCID:
PMC3151127
Michaela Koháryová, Jiří Brynda, Pavlína Řezáčová, Marta Kollárová

Thioredoxin reductase from S. coelicolor was crystallized and diffraction data were collected to 2.4 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 917–921. Published online 2011 July 20. doi: 10.1107/S1744309111021385
PMCID:
PMC3151128
Alexandre Ciaccafava, Audrey Lartigue, Pascal Mansuelle, Sandra Jeudy, Chantal Abergel

The mimivirus L544 gene product was expressed in E. coli and crystallized; preliminary phasing of a MAD data set was performed using the selenium signal present in a crystal of recombinant selenomethionine-substituted protein.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 922–925. Published online 2011 July 20. doi: 10.1107/S1744309111020847
PMCID:
PMC3151129
Andrey V. Lyashenko, Oksana Belova, Azat G. Gabdulkhakov, Alexander A. Lashkov, Alexandr V. Lisov, Alexey A. Leontievsky, Al’bert M. Mikhailov

The purification, crystallization and preliminary X-ray structure analysis of the laccase from G. lucidum are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 926–929. Published online 2011 July 20. doi: 10.1107/S1744309111021129
PMCID:
PMC3151130
Byeong-Gu Han, Byung Il Lee

P. furiosus PF2050 has been crystallized. Diffraction data were collected to 1.56 Å resolution using synchrotron X-rays.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 930–932. Published online 2011 July 20. doi: 10.1107/S1744309111023888
PMCID:
PMC3151131
Jiang Yin, Grace Garen, Craig Garen, Michael N. G. James

The small regulatory subunit of acetohydroxylate synthase (IlvH) from M. tuberculosis has been crystallized and preliminary crystallographic analysis has been performed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 933–936. Published online 2011 July 20. doi: 10.1107/S1744309111021105
PMCID:
PMC3151132
Pei-Hsiu Wu, Jye-Jin Hsu, Ting-Wei Chiang, Yin-Cheng Hsieh, Hwan-You Chang, Shou-Lin Chang, Chun-Jung Chen

The crystallization of PA3346RS from P. aeruginosa is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 937–940. Published online 2011 July 21. doi: 10.1107/S1744309111023931
PMCID:
PMC3151133
Wenzheng Zhang, Wei Peng, Mingzhuo Zhao, Dejun Lin, Zonghao Zeng, Weihong Zhou, Mark Bartlam

Human Thrsp has been crystallized as a prelude to the determination of its three-dimensional structure by X-ray crystallography.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 941–946. Published online 2011 July 26. doi: 10.1107/S1744309111021099
PMCID:
PMC3151134
Quincy Tseng, Jillian Orans, Michael A. Hast, Ravi R. Iyer, Anita Changela, Paul L. Modrich, Lorena S. Beese

Human MutSβ is a 232 kDa heterodimer (MSH2–MSH3) involved in the lesion-recognition step of mismatch repair. Here, the overexpression, purification, biochemical characterization and cocrystallization of MutSβ with a duplex DNA substrate are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 947–952. Published online 2011 July 26. doi: 10.1107/S1744309111019300
PMCID:
PMC3151135
Rui Qiu, Fengbin Wang, Meiruo Liu, Zhenxing Yang, Tong Wu, Chaoneng Ji

Yeast tRNA-thiouridine modification protein 1 was overpressed in E. coli, purified and crystallized. The crystals belonged to space group I41 and diffracted to a resolution of 1.9 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 953–955. Published online 2011 July 27. doi: 10.1107/S1744309111024900
PMCID:
PMC3151136
Honghua Ge, Peisong Xu, Ying Xu, Zemin Fang, Yazhong Xiao

Lac15 from a marine microbial metagenome has been crystallized and preliminary crystallographic analysis has been performed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 956–958. Published online 2011 July 27. doi: 10.1107/S1744309111024912
PMCID:
PMC3151137
Jiang Yin, Craig R. Garen, Katherine Bateman, Minmin Yu, Emily Z. Alipio Lyon, Jeff Habel, Heungbok Kim, Li-wei Hung, Chang-Yub Kim, Michael N. G. James

O-Acetylhomoserine sulfhydrylase from M. tuberculosis H37Rv has been crystallized and preliminary X-ray crystallographic analysis has been performed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 959–963. Published online 2011 July 27. doi: 10.1107/S1744309111017611
PMCID:
PMC3151138
Ruimin Zhou, Cong Huang, Aili Zhang, Stephen G. Bell, Weihong Zhou, Luet-Lok Wong

The expression, purification and preliminary crystallographic analysis of CYP153C1, an alkane-binding cytochrome P450 enzyme, are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 964–967. Published online 2011 July 27. doi: 10.1107/S174430911102464X
PMCID:
PMC3151139
Tingting Ran, Yu Wang, Dongqing Xu, Weiwu Wang

To elucidate the mechanism of oxaloacetate decarboxylation by Cg1458, recombinant Cg1458 has been purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 968–970. Published online 2011 July 27. doi: 10.1107/S1744309111023220
PMCID:
PMC3151140

Laboratory Communications

Vivian Stojanoff, Jean Jakoncic, Deena A. Oren, V. Nagarajan, Jens-Christian Navarro Poulsen, Melanie A. Adams-Cioaba, Terese Bergfors, Morten O. A. Sommer

Microfluidic crystallization using the Crystal Former improves the identification of initial crystallization conditions relative to screening via vapour diffusion.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 971–975. Published online 2011 July 26. doi: 10.1107/S1744309111024456
PMCID:
PMC3151141

Addenda and Errata

Jae-Woo Ahn, Shin Youp Lee, Sangwoo Kim, Sun Ja Cho, Sun Bok Lee, Kyung-Jin Kim

An addendum to the article by Ahn et al. [Acta Cryst. (2011) F67, 689–691].

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 976. Published online 2011 July 13. doi: 10.1107/S1744309111025887
PMCID:
PMC3151142
Ae Kyung Park, Kyoung Sun Park, Hak Jun Kim, Hyun Park, In Young Ahn, Young Min Chi, Jin Ho Moon

A correction to the article by Park et al. [(2011). Acta Cryst. F67, 800–802].

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 August 1; 67(Pt 8): 977. Published online 2011 July 13. doi: 10.1107/S1744309111027060
PMCID:
PMC3151143

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography