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Volume 66(Pt 8);  2010 August 1

Structural Communications

Boguslaw Stec, Anton Cheltsov, José Luis Millán

Re-refinement of three models of human placental alkaline phosphatase resulted in improved models for the protein and led to proper definition of the bound ligands. The PNP molecules found in the new models have consistent interactions at the original peripheral site and an additional peripheral site.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 866–870. Published online 2010 July 27. doi: 10.1107/S1744309110019767
PMCID:
PMC2917279
Emerich Mihai Gazdag, Ion Cristian Cirstea, Reinhard Breitling, Julius Lukeš, Wulf Blankenfeldt, Kirill Alexandrov

The structures of two new crystal forms of human Cu/Zn superoxide dismutase produced in the eukaryotic expression host L. tarentolae are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 871–877. Published online 2010 July 27. doi: 10.1107/S1744309110019330
PMCID:
PMC2917280
Markus Alahuhta, Qi Xu, Roman Brunecky, William S. Adney, Shi-You Ding, Michael E. Himmel, Vladimir V. Lunin

The 1.6 Å resolution structure of a fibronectin type III-like module from Clostridium thermocellum (PDB code 3mpc) with two molecules in the asymmetric unit is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 878–880. Published online 2010 July 27. doi: 10.1107/S1744309110022529
PMCID:
PMC2917281
Stéphane Réty, Lenka Řežábková, Barbara Dubanchet, Jan Šilhán, Pierre Legrand, Anita Lewit-Bentley

The crystal structure of the catalytic core of the primate foamy virus integrase was solved to 2 Å resolution and in several different crystal forms. The effect of divalent cations and of mutations on the structure is discussed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 881–886. Published online 2010 July 27. doi: 10.1107/S1744309110022852
PMCID:
PMC2917282
Prem Singh Kaushal, Ramappa K. Talawar, Umesh Varshney, M. Vijayan

The molecule of uracil-DNA glycosylase from M. tuberculosis exhibits domain motion on binding to DNA or a proteinaceous inhibitor. The highly conserved DNA-binding region interacts with a citrate ion in the structure.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 887–892. Published online 2010 July 27. doi: 10.1107/S1744309110023043
PMCID:
PMC2917283
Mayumi Kanagawa, Seiki Baba, Akio Ebihara, Akeo Shinkai, Ken Hirotsu, Ryosuke Mega, Kwang Kim, Seiki Kuramitsu, Gen-ichi Sampei, Gota Kawai

Structures of hypoxanthine-guanine phosphoribosyltransferase from T. thermophilus HB8 in the unliganded form, in complex with IMP and in complex with GMP are reported at 2.1, 1.9 and 2.2 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 893–898. Published online 2010 July 27. doi: 10.1107/S1744309110023079
PMCID:
PMC2917284

Crystallization Communications

Matt Horsham, Harriet Saxby, James Blake, Neil W. Isaacs, Tim J. Mitchell, Alan Riboldi-Tunnicliffe

Purified transketolase from L. salivarius has been crystallized using the vapour-diffusion technique.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 899–901. Published online 2010 July 27. doi: 10.1107/S1744309110012157
PMCID:
PMC2917285
Jing Li, Xiaojiao Xue, Jianbin Ruan, Minhao Wu, Zhiqiang Zhu, Jianye Zang

The tandem tudor domain of Sgf29 from S. cerevisiae has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 1.92 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 902–904. Published online 2010 July 27. doi: 10.1107/S1744309110016726
PMCID:
PMC2917286
Hyung Ho Lee, Se Won Suh

MnmE from P. aeruginosa was crystallized and X-ray diffraction data were collected to 2.69 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 905–908. Published online 2010 July 27. doi: 10.1107/S1744309110018750
PMCID:
PMC2917287
Xia Cheng, Guangwen Lu, Jianxun Qi, Hao Cheng, Feng Gao, Jundong Wang, Jinghua Yan

Crystals of SAICAR synthase from S. suis serotype 2 were obtained in the presence of 40 mM aspartic acid substrate; they belonged to space group P2 and diffracted to 2.8 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 909–912. Published online 2010 July 27. doi: 10.1107/S1744309110020518
PMCID:
PMC2917288
Xiaotian Liu, Minhao Wu, Demeng Sun, Jianye Zang

The E. coli transcription repressor LsrR has been overexpressed, purified and crystallized. Diffraction data were collected to about 3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 913–915. Published online 2010 July 27. doi: 10.1107/S1744309110020695
PMCID:
PMC2917289
Tom J. Petty, Taisuke Nishimura, Soheila Emamzadah, Caroline Gabus, Jerzy Paszkowski, Thanos D. Halazonetis, Stéphane Thore

In order to investigate its function in transcriptional gene silencing, the highly conserved motif 2 from A. thaliana Morpheus’ molecule 1 protein was expressed, purified and crystallized. X-ray diffraction analysis is reported to a resolution of 3.2 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 916–918. Published online 2010 July 29. doi: 10.1107/S1744309110021068
PMCID:
PMC2917290
Mary Rose Tandang-Silvas, Laura Carrazco-Peña, Ana Paulina Barba de la Rosa, Juan Alberto Osuna-Castro, Shigeru Utsumi, Bunzo Mikami, Nobuyuki Maruyama

High level expression of soluble amaranth 11S proglobulin in Escherichia coli and its purification are described. Crystallization of the recombinant protein and crystal data collection are also presented.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 919–922. Published online 2010 July 29. doi: 10.1107/S1744309110021032
PMCID:
PMC2917291
Tae-ho Jang, Bokyung Kim, Ok Kyoung Park, Ju Young Bae, Byung-Gee Kim, Hyungdon Yun, Hyun Ho Park

The omega-transaminase from V. fluvialis JS17 was crystallized. Crystals were found to belong to the orthorhombic space group P212121, with unit-cell parameters a = 78.43, b = 95.95, c = 122.89 Å. The crystals were obtained at 293 K and diffracted to a resolution of 2.5 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 923–925. Published online 2010 July 29. doi: 10.1107/S1744309110021573
PMCID:
PMC2917292
Zhen Xu, Weili Yang, Nuo Shi, Yongxiang Gao, Maikun Teng, Liwen Niu

The SET/TAF-Iβ that lacked the first 22 residues of the N-terminus from Homo sapiens was recombinantly expressed in Escherichia coli and crystallized. X-ray diffraction data were collected to 2.7 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 926–928. Published online 2010 July 29. doi: 10.1107/S1744309110021779
PMCID:
PMC2917293
Jiang Gu, Xiaowei Ji, Jianxun Qi, Ying Ma, Xuhu Mao, Quanming Zou

In this study, recombinant OmpAC from EHEC was purified and crystallized and a diffraction data set was collected to 2.7 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 929–931. Published online 2010 July 29. doi: 10.1107/S1744309110016891
PMCID:
PMC2917294
Yan Lin, Ping Wang, Huirong Yang, Yanhui Xu

Crystallization of the ADP-ribosyltransferase HopU1 is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 932–934. Published online 2010 July 29. doi: 10.1107/S1744309110022463
PMCID:
PMC2917295
Guoming Li, Linglong Qu, Geng Meng, Xiaoyun Bai, Kesheng Dai, Xiaofeng Zheng

Full-length human Fas apoptosis inhibitory molecule (FAIM) and two truncation constructs have successfully been cloned, expressed and purified in Escherichia coli. FAIM (1–90) was crystallized and diffracted to a resolution of 2.5 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 935–937. Published online 2010 July 29. doi: 10.1107/S1744309110022657
PMCID:
PMC2917296
Paul T. Harris, Kannan Raghunathan, Rachel R. Spurbeck, Cindy G. Arvidson, Dennis N. Arvidson

Recombinant L. jensenii enolase has been expressed, purified and crystallized. The crystals diffracted to 3.25 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 938–940. Published online 2010 July 29. doi: 10.1107/S1744309110022748
PMCID:
PMC2917297
Ryuichiro Suzuki, Byung-Jun Kim, Tsuyoshi Shibata, Yuki Iwamoto, Takane Katayama, Hisashi Ashida, Takayoshi Wakagi, Hirofumi Shoun, Shinya Fushinobu, Kenji Yamamoto

Xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from B. breve was overexpressed and crystallized. The crystals belonged to the tetragonal space group I422 and diffracted to beyond 1.7 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 941–943. Published online 2010 July 29. doi: 10.1107/S1744309110023845
PMCID:
PMC2917298
Susmita Khamrui, Maitree Biswas, Udayaditya Sen, Jhimli Dasgupta

A chemotaxis response regulator CheY3 from V. cholerae has been cloned, overexpressed, purified and crystallized. The crystals of CheY3 diffracted to 1.86 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 944–947. Published online 2010 July 29. doi: 10.1107/S1744309110022797
PMCID:
PMC2917299
Hiroyuki Morita, Kiyofumi Wanibuchi, Ryohei Kato, Shigetoshi Sugio, Ikuro Abe

Curcuminoid synthase from O. sativa has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 2.5 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 948–950. Published online 2010 July 29. doi: 10.1107/S1744309110023572
PMCID:
PMC2917300
Weijie You, Xiaoting Qiu, Yujie Zhang, Jinming Ma, Yongxiang Gao, Xiao Zhang, Liwen Niu, Maikun Teng

The putative aldose 1-epimerase YeaD from Escherichia coli was crystallized and diffraction data were collected to a resolution of 1.9 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 951–953. Published online 2010 July 29. doi: 10.1107/S1744309110024140
PMCID:
PMC2917301
Lu Lu, Jie Nan, Wei Mi, Chun-Hong Wei, Lan-Fen Li, Yi Li

Tubulin-folding cofactor A from A. thaliana has been crystallized and preliminarily analyzed using X-ray diffraction.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 954–956. Published online 2010 July 29. doi: 10.1107/S1744309110023900
PMCID:
PMC2917302
Lisandro H. Otero, Paola R. Beassoni, Carlos E. Domenech, Angela T. Lisa, Armando Albert

Phosphorylcholine phosphatase from P. aeruginosa has been crystallized. Diffraction data have been collected to 2.7 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 957–960. Published online 2010 July 29. doi: 10.1107/S1744309110024061
PMCID:
PMC2917303
Irene Russo Krauss, Antonello Merlino, Antonio Randazzo, Lelio Mazzarella, Filomena Sica

The complex between human α-thrombin and a modified thrombin-binding aptamer has been crystallized. Diffraction data were collected to 2.15 Å resolution, the structure was solved by molecular replacement and refinement of the model is in progress.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 961–963. Published online 2010 July 29. doi: 10.1107/S1744309110024632
PMCID:
PMC2917304
Stefan Harth, Alexander Kotzsch, Walter Sebald, Thomas Dieter Mueller

The crystallization of BMP receptor type IA bound to the neutralizing antibody Fab fragment AbD1556 obtained by phage-display selection is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 964–968. Published online 2010 July 29. doi: 10.1107/S1744309110024681
PMCID:
PMC2917305

Laboratory Communications

Neela Yennawar, Sava Denev, Venkataraman Gopalan, Hemant Yennawar

The effect of laser irradiation on freshly prepared protein drops was explored and found to be a viable tool to induce nucleation. In our experiment, the number of positive crystallization hits during the screening process increased. In some instances the speed of crystal growth, the size of the crystals and the resolution of X-ray diffraction showed a marked improvement after laser exposure. Under

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 August 1; 66(Pt 8): 969–972. Published online 2010 July 30. doi: 10.1107/S1744309110023857
PMCID:
PMC2917306

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography