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Volume 66(Pt 5);  2010 May 1

Structural Communications

Tatsuki Ebisawa, Akihiro Yamamura, Yasuhiro Kameda, Kou Hayakawa, Koji Nagata, Masaru Tanokura

The crystal structure of a monomeric mutant of Azami-Green (mAG) from G. fascicularis was determined at 2.2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 485–489. Published online 2010 April 29. doi: 10.1107/S1744309110011127
PMCID:
PMC2864674
Kanchan Anand, Divya Mathur, Avishek Anant, Lalit C. Garg

The first crystal structure of phosphoglucose isomerase from M. tuberculosis H37Rv is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 490–497. Published online 2010 April 29. doi: 10.1107/S1744309110011656
PMCID:
PMC2864675
Chao-Pei Liu, Rui Xu, Zeng-Qiang Gao, Jian-Hua Xu, Hai-Feng Hou, Li-Qin Li, Zhun She, Lan-Fen Li, Xiao-Dong Su, Peng Liu, Yu-Hui Dong

The crystal structure of OPRTase from the caries pathogen Streptococcus mutans is reported at 2.4 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 498–502. Published online 2010 April 29. doi: 10.1107/S1744309110009243
PMCID:
PMC2864676

Crystallization Communications

Daiki Ogata, Toshihiko Ooi, Takaaki Fujiwara, Seiichi Taguchi, Isao Tanaka, Min Yao

In order to determine the structure–function relationship of the azo-dye reduction mechanism, an X-ray crystallographic study of azoreductases was performed. Selenomethionine-labelled AzrA (SeMet-AzrA) and AzrC were crystallized by the hanging-drop vapour-diffusion method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 503–505. Published online 2010 April 29. doi: 10.1107/S1744309110007785
PMCID:
PMC2864677
Somnath Mukherjee, Baisakhee Saha, Debajyoti Dutta, Amit Kumar Das

The purification, crystallization and preliminary X-ray crystallographic analysis of glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) from MRSA252 in the apo form is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 506–508. Published online 2010 April 29. doi: 10.1107/S1744309110007980
PMCID:
PMC2864678
Ved Prakash Dubey, Biswajit Pal, Subramanya Srikantan, Sambhavi Pottabathini, Prabir Kumar De, Rajan Sankaranarayanan

A female-specific lacrimal protein from Syrian hamsters has been crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P212121 and diffraction data were collected to 1.86 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 509–512. Published online 2010 April 29. doi: 10.1107/S1744309110008237
PMCID:
PMC2864679
Xiaoli Zhang, Jinyong Zhang, Xuhu Mao, Quanming Zou, Yonglin Hu, Da-Cheng Wang

Crystals of C-terminally His-tagged ThyX from H. pylori belonged to space group C2, with unit-cell parameters a = 221.92, b = 49.43, c = 143.02 Å, β = 98.84°, and diffracted to beyond 2.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 513–515. Published online 2010 April 29. doi: 10.1107/S174430911000864X
PMCID:
PMC2864680
Wenbing Tao, Feng Li, Haiping Liu, Xiangyu Bao, Weimin Gong, Shaoning Yu

N-terminal fragments of the cyclic AMP receptor protein from E. coli created by two different proteases, subtilisin and chymotrypsin, have been crystallized and diffracted to 2.0 and 2.8 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 516–519. Published online 2010 April 29. doi: 10.1107/S1744309110008675
PMCID:
PMC2864681
Tetsuya Kohno, Hiroto Yamaguchi, Toshio Hakoshima

The endonuclease/exonuclease/phosphatase-homology domain of phosphodiesterase PDE12 has been crystallized. The crystals diffracted to 2.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 520–522. Published online 2010 April 29. doi: 10.1107/S1744309110008766
PMCID:
PMC2864682
Tania F. Oliveira, John B. Carrigan, Muaawia A. Hamza, Michael A. Sharkey, Paul C. Engel, Amir R. Khan

Selenomethionine-derivatized glutamate dehydrogenase from P. asaccharolyticus has been crystallized. Diffraction data were collected to 3.5 Å resolution from crystals belonging to the rhombohedral space group H32 and structure determination is in progress.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 523–526. Published online 2010 April 29. doi: 10.1107/S1744309110010006
PMCID:
PMC2864683
Gil Bu Kang, Hye-Eun Song, Mun-Kyoung Kim, Hyung-Seop Youn, Jun Yop An, Jung-Gyu Lee, Kyung Ryung Park, Soo Hyun Eom

H. pylori MinE has been expressed, purified and crystallized. Native and MAD data sets have been collected from H. pylori MinE crystals to 2.8 and 3.0 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 527–529. Published online 2010 April 29. doi: 10.1107/S1744309110009784
PMCID:
PMC2864684
Wang-Hong Zhao, Xiu-Rong Zhan, Xiong-Zhuo Gao, Xiang Liu, Yi-Fei Zhang, Jiuxiang Lin, Lan-Fen Li, Shi-Cheng Wei, Xio-Dong Su

The SMU.2055 gene from the major caries pathogen Streptococcus mutans was cloned and native and SeMet-labelled SMU.2055 proteins were expressed at a high level. Diffraction-quality crystals of SeMet-labelled SMU.2055 were obtained using the sitting-drop vapour-diffusion method and diffracted to a resolution of 2.5 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 530–533. Published online 2010 April 29. doi: 10.1107/S1744309110010365
PMCID:
PMC2864685
Pramod K. Madoori, Andy-Mark W. H. Thunnissen

Two soluble His-tagged forms of the lytic transglycosylase MltF, one containing full length protein, the other containing its N-terminal domain, have been successfully overexpressed, purified and crystallized. X-ray diffraction data were collected for both crystal forms, including a MAD data set to 2.7 Å for the MltF N-terminal domain.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 534–538. Published online 2010 April 29. doi: 10.1107/S1744309110010596
PMCID:
PMC2864686
Hyun Koo Yeo, Jae Young Lee

Triclinic crystals of a self-complementary DNA heptacosamer with 20-base-pair duplexes flanked by 3′-terminal seven-nucleotide overhangs have been obtained. X-ray data were collected to 2.8 Å resolution using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 539–541. Published online 2010 April 29. doi: 10.1107/S1744309110010687
PMCID:
PMC2864687
Ana Lucia L. R. Zimbardi, Matheus P. Pinheiro, Marcelo Dias-Baruffi, M. Cristina Nonato

The N-terminal carbohydrate-recognition domain of human galectin-4 has been crystallized by the vapour-diffusion technique using polyethylene glycol as the precipitant agent. A complete data set was collected from a native crystal to 2.2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 542–545. Published online 2010 April 29. doi: 10.1107/S1744309110010778
PMCID:
PMC2864688
Tat-Cheung Cheng, Yu Wai Chen, Kam-Bo Wong, H. Y. Edwin Chan

A soluble domain spanning amino-acid residues 16–197 of the Drosophila Dribble protein was overexpressed in E. coli, purified and crystallized. X-ray diffraction data were collected to beyond 2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 546–548. Published online 2010 April 29. doi: 10.1107/S1744309110011206
PMCID:
PMC2864689
José Ignacio Jiménez, Iván Acebrón, José Luis García, Eduardo Díaz, José Miguel Mancheño

The Fe2+-dependent dioxygenase NicX from P. putida KT2440 has been crystallized using the vapour-diffusion method. Native data have been collected to 2.0 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 549–553. Published online 2010 April 29. doi: 10.1107/S174430911001119X
PMCID:
PMC2864690
Priadarsini Subburaman, Brian P. Austin, Gary X. Shaw, David S. Waugh, Xinhua Ji

The macrophage growth locus A (MglA) protein from F. tularensis crystallized in the hexagonal space group P61 or P65, with unit-cell parameters a = b = 125, c = 54 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 554–557. Published online 2010 April 29. doi: 10.1107/S1744309110009711
PMCID:
PMC2864691
Bärbel Kaufmann, Pavel Plevka, Richard J. Kuhn, Michael G. Rossmann

Crystals of infectious West Nile virus were obtained and diffracted at best to about 25 Å resolution. Preliminary analysis of the diffraction pattern suggested tight hexagonal packing of the intact virus.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 558–562. Published online 2010 April 30. doi: 10.1107/S1744309110009899
PMCID:
PMC2864692
Seiki Baba, Tatsuhiko Someya, Gota Kawai, Kouji Nakamura, Takashi Kumasaka

The RNA-binding protein Hfq from B. subtilis was crystallized using the hanging-drop vapour-diffusion method in two crystal forms that belonged to space groups I422 and F222; diffraction data were collected to 2.2 Å resolution from both forms.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 563–566. Published online 2010 April 30. doi: 10.1107/S1744309110009942
PMCID:
PMC2864693
Simone Culurgioni, Inés G. Muñoz, Alicia Palacios, Pilar Redondo, Francisco J. Blanco, Guillermo Montoya

The N-terminal domain of ING4 was overexpressed in Escherichia coli and purified to homogeneity. Crystallization experiments yielded crystals that were suitable for high-resolution X-ray diffraction analysis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 567–570. Published online 2010 April 30. doi: 10.1107/S1744309110010080
PMCID:
PMC2864694
Lídia Barata, Marta Sousa Silva, Linda Schuldt, Gonçalo da Costa, Ana M. Tomás, António E. N. Ferreira, Manfred S. Weiss, Ana Ponces Freire, Carlos Cordeiro

Glyoxalase I from L. infantum was cloned, overexpressed in E. coli, purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 571–574. Published online 2010 April 30. doi: 10.1107/S1744309110010754
PMCID:
PMC2864695
Sandeep Chhabra, Janet Newman, Thomas S. Peat, Ross T. Fernley, Joanne Caine, Jamie S. Simpson, James D. Swarbrick

The expression, purification and crystallization of S. aureus 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, an essential enzyme from the folate-biosynthesis pathway, is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 575–578. Published online 2010 April 30. doi: 10.1107/S1744309110010857
PMCID:
PMC2864696
Huirong Yang, Ping Wang, Zhenghong Dong, Xueyuan Li, Rui Gong, Ying Yang, Ze Li, Youwei Xu, Yanhui Xu

The expression, purification and crystallization of nosiheptide-resistance methyltransferase (NSR) from Streptomyces actuosus is described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 579–582. Published online 2010 April 30. doi: 10.1107/S1744309110011395
PMCID:
PMC2864697
Jani Huttu, Bishal Kumar Singh, Saligram Prabhakar Bhargav, Julia M. Sattler, Herwig Schüler, Inari Kursula

The expression, purification and crystallization of Plasmodium actin-depolymerization factors 1 and 2 are described. X-ray diffraction data were collected to 2.0 and 2.1 Å resolution, respectively, and the structures of both proteins in solution were characterized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 583–587. Published online 2010 April 30. doi: 10.1107/S1744309110011589
PMCID:
PMC2864698
Joel T. Weadge, Patrick P. Yip, Howard Robinson, Krista Arnett, Peter A. Tipton, P. Lynne Howell

The expression, purification and crystallization of the periplasmic protein AlgX from P. aeruginosa is described. The crystals diffracted to 2.1 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 588–591. Published online 2010 April 30. doi: 10.1107/S1744309110011851
PMCID:
PMC2864699
Mads Gabrielsen, Puteri Shafinaz Abdul-Rahman, Neil W. Isaacs, Onn Haji Hashim, Richard J. Cogdell

Champedak mannose-binding lectin was crystallized at 293 K. Crystallization conditions and preliminary X-ray diffraction analysis are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 592–594. Published online 2010 April 30. doi: 10.1107/S1744309110011760
PMCID:
PMC2864700
HaJeung Park, Erumbi S. Rangarajan, Jurgen Sygusch, Tina Izard

Rabbit muscle aldolase (RMA) was crystallized in complex with the low-complexity domain (LC4) of sorting nexin 9. Monoclinic crystals were obtained at room temperature that displayed large mosaicity and poor X-ray diffraction. However, orthorhombic RMA–LC4 crystals grown at 277 K under similar conditions exhibited low mosaicity, allowing data collection to 2.2 Å Bragg

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 595–600. Published online 2010 April 30. doi: 10.1107/S1744309110011875
PMCID:
PMC2864701
R. Jarrott, S. R. Shouldice, G. Gunčar, M. Totsika, M. A. Schembri, B. Heras

The cloning, purification, crystallization and preliminary crystallographic studies of three DsbA-like proteins present in S. enterica serovar Typhimurium, SeDsbA, SeDsbL and SeSrgA, are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 601–604. Published online 2010 April 30. doi: 10.1107/S1744309110011942
PMCID:
PMC2864702
Filipa G. Pinho, Célia V. Romão, Ana F. Pinto, Lígia M. Saraiva, Harald Huber, Pedro M. Matias, Miguel Teixeira, Tiago M. Bandeiras

Diffraction-quality crystals of I. hospitalis neelaredoxin have been produced. The cloning, expression, purification, crystallization and X-ray crystallographic analysis are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 605–607. Published online 2010 April 30. doi: 10.1107/S1744309110012030
PMCID:
PMC2864703
Nipawan Nuemket, Yoshikazu Tanaka, Kentaro Tsukamoto, Takao Tsuji, Keiji Nakamura, Shunji Kozaki, Min Yao, Isao Tanaka

To determine the binding mechanism of BoNT/OFD05 and its ganglioside receptors on neuronal cells, recombinant BoNT/OFD05 receptor-binding domain has been expressed, purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 608–610. Published online 2010 April 30. doi: 10.1107/S1744309110012182
PMCID:
PMC2864704
Gesa Volkers, Linda Schuldt, Gottfried J. Palm, Gerard D. Wright, Winfried Hinrichs

Crystallization and preliminary X-ray crystallographic analysis of the tetracycline-degrading monooxygenase TetX2 from B. thetaiotaomicron are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 611–614. Published online 2010 April 30. doi: 10.1107/S174430911001225X
PMCID:
PMC2864705

Laboratory Communications

Kannan Raghunathan, Paul T. Harris, Dennis N. Arvidson

A simple ‘melt test’ to distinguish salt crystals from macromolecule crystals is described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5): 615–620. Published online 2010 April 29. doi: 10.1107/S1744309110012078
PMCID:
PMC2864706

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography