PMCCPMCCPMCC

Search tips
Search criteria 

Advanced
Logo of actafjournal home pageInternational Union of Crystallographysearchsubscribearticle submissionthis issue
 
This journal provides early access to some articles.

Volume 66(Pt 4);  2010 April 1

Structural Communications

Amit Meir, Oded Livnah

The structure of the rhizavidin–biotin complex was determined via combined molecular replacement approaches. Both the apo and biotin complexed structures pack in unique oligomeric states in the corresponding crystals.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 373–378. Published online 2010 March 26. doi: 10.1107/S1744309110004112
PMCID:
PMC2852325
Marc-Michael Blum, Stephen J. Tomanicek, Harald John, B. Leif Hanson, Heinz Rüterjans, Benno P. Schoenborn, Paul Langan, Julian C.-H. Chen

The crystal structure of perdeuterated diisopropyl fluorophosphatase is reported and compared with the hydrogenated structure. Diffraction guidelines for neutron crystallography experiments are summarized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 379–385. Published online 2010 March 26. doi: 10.1107/S1744309110004318
PMCID:
PMC2852326
Andrey Galkin, Liudmila Kulakova, Rui Wu, Theodore E. Nash, Debra Dunaway-Mariano, Osnat Herzberg

The three-dimensional structure of carbamate kinase from the human parasite G. lamblia has been determined at 3 Å resolution. Steady-state kinetic parameters have been determined and dsRNA-silencing experiments indicate that the enzyme is essential for the survival of G. lamblia.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 386–390. Published online 2010 March 26. doi: 10.1107/S1744309110004665
PMCID:
PMC2852327
John Beale, Sang Yun Lee, So Iwata, Konstantinos Beis

Sulfur is an essential component for the biosynthesis of the sulfur-containing amino acids l-methionine and l-cysteine. The crystal structure of the periplasmic aliphatic sulfonate-binding protein SsuA from E. coli has been solved at 1.75 Å resolution in a substrate-free state. Comparison with the substrate-bound protein shows significant movement of one of the two domains.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 391–396. Published online 2010 March 26. doi: 10.1107/S1744309110006226
PMCID:
PMC2852328
Charles B. C. Cielo, Seiji Okazaki, Atsuo Suzuki, Tsunehiro Mizushima, Ryoji Masui, Seiki Kuramitsu, Takashi Yamane

The structure of ST0929 provides insight into the structural basis of the increase in the hydrolase side reaction that is observed for mutants in which a phenylalanine residue is replaced by a tyrosine residue in the subsite +1 tyrosine cluster of Sulfolobus sp.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 397–400. Published online 2010 March 26. doi: 10.1107/S1744309110006354
PMCID:
PMC2852329

Crystallization Communications

S. Caria, D. de Sanctis, F. J. Enguita, S. McSweeney

The expression, purification and crystallization of a catalytic and ATP-binding domain of PhoR histidine kinase from D. radiodurans is described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 401–404. Published online 2010 March 31. doi: 10.1107/S1744309109054220
PMCID:
PMC2852330
Sarah Batson, Dean Rea, Vilmos Fülöp, David I. Roper

E. coli d-alanyl-d-alanine ligase has been crystallized in complex with ADP and d-alanyl-d-alanine and in complex with ATP and d-alanyl-d-alanine.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 405–408. Published online 2010 March 31. doi: 10.1107/S1744309110003970
PMCID:
PMC2852331
Rishabh Jain, Rositsa Jordanova, Ingrid B. Müller, Carsten Wrenger, Matthew R. Groves

The collection of X-ray diffraction data from the aspartate aminotransferase of P. falciparum to a resolution of 2.8 Å is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 409–412. Published online 2010 March 31. doi: 10.1107/S1744309110003933
PMCID:
PMC2852332
Jan-Hendrik Hehemann, Gurvan Michel, Tristan Barbeyron, Mirjam Czjzek

A novel family GH16 β-agarase from the marine bacterium Zobellia galactanivorans was expressed, purified and crystallized. Hexagonal crystals belonging to space group P3121 diffracted to 2.2 Å resolution, whereas orthorhombic crystals belonging to space group P212121 diffracted to 1.5–Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 413–417. Published online 2010 March 31. doi: 10.1107/S174430911000429X
PMCID:
PMC2852333
Bernhard Sielaff, Ki Seog Lee, Francis T. F. Tsai

The crystallization and preliminary X-ray crystallographic analysis of a 23 kDa GroEL1 fragment from M. tuberculosis H37Rv is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 418–420. Published online 2010 March 31. doi: 10.1107/S1744309110004409
PMCID:
PMC2852334
Paul G. Leonard, Gary N. Parkinson, Jayesh Gor, Stephen J. Perkins, John E. Ladbury

Crystals of the oligomerization domain of the S. typhimurium histone-like nucleoid-structuring protein were obtained that diffracted X-rays to a resolution of at least 4.0 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 421–425. Published online 2010 March 31. doi: 10.1107/S1744309110004574
PMCID:
PMC2852335
Zhijie Li, Yujia Zhai, Junnan Fang, Qiangjun Zhou, Yunqi Geng, Fei Sun

Very-long-chain acyl-CoA dehydrogenase from Caenorhabditis elegans (cVLCAD) has been crystallized in space group C2 and its X-ray diffraction data set has been collected to 1.6 Å resolution. Unlike other VLCADs that were reported to form dimers, the purified cVLCAD was found as a homotetrameric protein according to static light-scattering measurements.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 426–430. Published online 2010 March 31. doi: 10.1107/S1744309110005002
PMCID:
PMC2852336
Tomáš Klumpler, Vojtěch Sedláček, Jaromír Marek, Michaela Wimmerová, Igor Kučera

The flavin-dependent enzyme FerB from P. denitrificans has been purified and both native and SeMet-substituted FerB have been crystallized. The two variants crystallized in two different crystallographic forms belonging to the monoclinic space group P21 and the orthorhombic space group P21212, respectively. X-ray diffraction data were collected to 1.75 Å resolution for both forms.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 431–434. Published online 2010 March 31. doi: 10.1107/S1744309110005099
PMCID:
PMC2852337
Lili Zong, Yong Chen, Jinghua Yan, Jianhua Zhang

CD8α exodomain protein, a crucial immune-system factor in rhesus macaque (M. mulatta), one of the best animal models for vaccine design, was assembled and crystallized. The full structure data will contribute to future studies of immune responses in rhesus macaques.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 435–438. Published online 2010 March 31. doi: 10.1107/S1744309110005178
PMCID:
PMC2852338
Ha Yun Jung, Kyung Ha Kim, Ji Hye Hyoung, Mi Ra Han, Hyun Kyoung Kim, Ki Jeung Lee, Yangmee Kim, Hak Jun Kim, Yong-Seok Heo

The GyrA subunit of DNA gyrase from C. psychrerythraea strain 34H was expressed, purified and crystallized. Diffraction data were collected to a resolution of 2.60 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 439–441. Published online 2010 March 31. doi: 10.1107/S1744309110005567
PMCID:
PMC2852339
Annelies Van Hoorebeke, Jan Stout, Ruben Van der Meeren, John Kyndt, Jozef Van Beeumen, Savvas N. Savvides

The inverting trehalose phosphorylase from Thermoanaerobacter sp. was crystallized and its structure was determined by molecular replacement. Its purification, crystallization and preliminary X-ray diffraction analysis are presented.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 442–447. Published online 2010 March 31. doi: 10.1107/S1744309110005749
PMCID:
PMC2852340
Inmaculada Pérez-Dorado, Reyes Sanles, Ana González, Pedro García, José L. García, Martín Martínez-Ripoll, Juan A. Hermoso

The complete pneumococcal autolysin LytC has been crystallized by the hanging-drop vapor-diffusion method. A SAD data set has been collected in-house from a Gd derivative up to 2.6 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 448–451. Published online 2010 March 31. doi: 10.1107/S1744309110006081
PMCID:
PMC2852341
Gordon Winter, Simon Dökel, Anne K. Jones, Patrick Scheerer, Norbert Krauss, Wolfgang Höhne, Bärbel Friedrich

The hydrogenase maturation factor HypF1 from R. eutropha H16 was successfully crystallized and data sets were collected to a maximum resolution of 1.65 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 452–455. Published online 2010 March 31. doi: 10.1107/S1744309110006196
PMCID:
PMC2852342
Nobuyuki Shibata, Megumi Kagiyama, Masahiro Nakagawa, Yoshinori Hirano, Toshio Hakoshima

Crystals of the Arabidopsis abscisic acid receptors PYL9/RCAR1, PYL5/RCAR8 and PYR1/RCAR11 have been obtained.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 456–459. Published online 2010 March 31. doi: 10.1107/S1744309110007037
PMCID:
PMC2852343
Margriet Ovaere, Arthur Van Aerschot, Mikhail Abramov, Piet Herdewijn, Luc Van Meervelt

The self-complementary octamer sequence GTGTACAC of the fully modified altritol nucleic acid has been crystallized. Diffraction data have been collected and processed to 3.0 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 460–462. Published online 2010 March 31. doi: 10.1107/S1744309110007050
PMCID:
PMC2852344
Ningning Zhuang, Kyung Hey Seo, Cong Chen, Hye-Lim Kim, Young Shik Park, Kon Ho Lee

Phenylalanine hydroxylase from D. discoideum has been crystallized. Diffraction data were collected to 2.07 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 463–466. Published online 2010 March 31. doi: 10.1107/S1744309110007220
PMCID:
PMC2852345
Sagar Chittori, D. K. Simanshu, H. S. Savithri, M. R. N. Murthy

2-Methylcitrate synthase from S. typhimurium was cloned, overexpressed, purified and crystallized. The crystals diffracted X-­rays to 2.4 Å resolution and belonged to the triclinic space group P1 (unit-cell parameters a = 92.068, b = 118.159, c = 120.659 Å, α = 60.84, β = 67.77, γ = 81.92°). Self-rotation function calculations

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 467–470. Published online 2010 March 31. doi: 10.1107/S1744309110007256
PMCID:
PMC2852346
Ho-Chang Ryu, Suk-Youl Park, Sung Haeng Lee, Jeong-Sun Kim

The crystallization of 2-keto-3-deoxy-6-phosphogluconate aldolase from Z. mobilis ZM4, a key enzyme in ethanol production, and collection of diffraction data to 1.80 Å resolution are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 471–473. Published online 2010 March 31. doi: 10.1107/S1744309110007323
PMCID:
PMC2852347
A. Y. Kovalevsky, S. Zoe Fisher, Sean Seaver, Marat Mustyakimov, Narayanasami Sukumar, Paul Langan, Timothy C. Mueser, B. Leif Hanson

Equine cyanomethemoglobin has been crystallized and X-ray and neutron diffraction data have been measured. Joint X-ray–neutron refinement is under way; the structural results should help to elucidate the differences between the hemoglobin R and T states.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 474–477. Published online 2010 March 31. doi: 10.1107/S1744309110007840
PMCID:
PMC2852348

Laboratory Communications

Karsten Dierks, Arne Meyer, Dominik Oberthür, Gert Rapp, Howard Einspahr, Christian Betzel

Excitation of intrinsic fluorescence at wavelengths longer than 300 nm is effective in the detection of protein crystals in crystallization trials set up in the most commonly used hardware.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 April 1; 66(Pt 4): 478–484. Published online 2010 March 27. doi: 10.1107/S1744309110007153
PMCID:
PMC2852349

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography