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Volume 67(Pt 3);  2011 March 1

Structural Communications

Matthew W. Vetting, Subray S. Hegde, Yong Zhang, John S. Blanchard

The pentapeptide repeat protein AlbG, provides self-resistance to the nonribosomally encoded hybrid polyketide-peptide termed albicidin. Analysis of the AlbG three-dimensional structure and the sequences of other pentapeptide repeat proteins that confer resistance to topiosomerase poisons suggests they have a similar dimer interface which may be critical to their interaction with topoisomerases.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 296–302. Published online 2011 February 18. doi: 10.1107/S1744309110053315
PMCID:
PMC3053150
Jean-Denis Docquier, Manuela Benvenuti, Vito Calderone, Gian-Maria Rossolini, Stefano Mangani

TEM-72 is a quadruple mutant of TEM-1 and shows extended-spectrum β-lactamase properties. The present structure shows the presence of a citrate anion bound to the TEM-72 active site and supports the use of polycarboxylates as a scaffold for the design of broad-spectrum inhibitors of serine β-lactamases.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 303–306. Published online 2011 February 18. doi: 10.1107/S1744309110054680
PMCID:
PMC3053151

Crystallization Communications

Tung B. K. Le, Clare E. M. Stevenson, Mark J. Buttner, David M. Lawson

Crystals of SimR, a TetR-like efflux pump repressor from S. antibioticus, were obtained and X-ray data were recorded to a resolution of 2.3 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 307–309. Published online 2011 February 18. doi: 10.1107/S1744309110053078
PMCID:
PMC3053152
Suk-Youl Park, Jeong-Hoh Park, Jeong-Sun Kim

The crystallization of an ASCH domain-containing protein from Z. mobilis ZM4 and the collection of diffraction data to 2.1 Å resolution are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 310–312. Published online 2011 February 18. doi: 10.1107/S1744309110053467
PMCID:
PMC3053153
Aya Watanabe, Kunio Hirata, Yoshinori Hagiwara, Yuko Yutani, Masakazu Sugishima, Masaki Yamamoto, Keiichi Fukuyama, Kei Wada

Biliverdin reductase (BVR) from Synechocystis sp. PCC6803 and its selenomethionine derivative were overexpressed and purified. X-ray diffraction data from an SeMet BVR microcrystal were collected to 3.0 Å resolution on microfocus beamline BL32XU at SPring-8.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 313–317. Published online 2011 February 18. doi: 10.1107/S1744309110053431
PMCID:
PMC3053154
Iker Oyenarte, María Lucas, Inmaculada Gómez García, Luis Alfonso Martínez-Cruz

Preliminary crystallographic analysis of the CBS domain protein MJ1004 from Methanocaldococcus jannaschii.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 318–324. Published online 2011 February 23. doi: 10.1107/S1744309110053479
PMCID:
PMC3053155
Kenneth Verstraete, Bert Remmerie, Jonathan Elegheert, Beatrice Lintermans, Guy Haegeman, Peter Vanhoenacker, Kathleen Van Craenenbroeck, Savvas N. Savvides

The preparative purification and crystallization of Flt3 receptor–ligand complexes are described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 325–331. Published online 2011 February 23. doi: 10.1107/S1744309111003319
PMCID:
PMC3053156
Sarah M. Tiggelaar, Estelle Mossou, Phil Callow, Shirley Callow, Susana C. M. Teixeira, Edward P. Mitchell, Anna Mitraki, V. Trevor Forsyth

The first high-angle neutron fibre diffraction results from an amyloid system are described. These were obtained using H2O/D2O isototopic replacement, and emphasise the ability of neutron fibre diffraction, used in conjunction with deuterium labelling approaches, to study novel aspects of amyloid conformation and hydration.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 332–335. Published online 2011 February 23. doi: 10.1107/S1744309111002351
PMCID:
PMC3053157
Natalie J. Gunn, Michael A. Gorman, Renwick C. J. Dobson, Michael W. Parker, Terrence D. Mulhern

The Src-homology 2 (SH2) domain of Csk-family protein tyrosine kinases acts as a conformational switch to regulate their catalytic activity, which in turn promotes the inhibition of their proto-oncogenic targets, the Src-family kinases. Here, the expression, purification, small-angle X-ray scattering and preliminary diffraction analysis of the SH2 domain of the Csk-homologous kinase is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 336–339. Published online 2011 February 23. doi: 10.1107/S1744309110053728
PMCID:
PMC3053158
Ahmed Akrem, Sadaf Iqbal, Friedrich Buck, Arne Meyer, Markus Perbandt, Wolfgang Voelter, Christian Betzel

Crystals of a chitinase from C. vernus were monoclinic, belonging to space group C2 with unit-cell parameters a = 172.3, b = 37.1, c = 126.4 Å, β = 127°, and diffracted to 2.1 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 340–343. Published online 2011 February 23. doi: 10.1107/S1744309110053698
PMCID:
PMC3053159
Hirotaka Narita, Atsushi Nakagawa, Yasunori Yamamoto, Toshiaki Sakisaka, Yoshimi Takai, Mamoru Suzuki

The whole extracellular regions of nectin-1 (nectin-1-EC) and nectin-2 (nectin-2-EC) were expressed in E. coli as inclusion bodies, solubilized in 8 M urea and then refolded by rapid dilution. Refolded nectin-1-EC and nectin-2-EC were subsequently purified using three chromatographic steps and crystallized by the hanging-drop vapour-diffusion method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 344–348. Published online 2011 February 23. doi: 10.1107/S174430911100337X
PMCID:
PMC3053160
Inmaculada Gómez García, Iker Oyenarte, Luis Alfonso Martínez-Cruz

This work describes the purification and preliminary crystallographic analysis of the CBS-pair regulatory domain of the human ancient domain protein 4 (ACDP4), also known as CNNM4.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 349–353. Published online 2011 February 23. doi: 10.1107/S1744309110053856
PMCID:
PMC3053161
Guillaume Gotthard, Julien Hiblot, Mikael Elias, Eric Chabrière

A lactonase from the hyperthermophilic archaeon S. islandicus has been crystallized. In combination with biochemical and bioengineering studies, it is expected that the structure of this protein will provide insight into the natural function of the phosphotriesterase-like lactonase family.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 354–357. Published online 2011 February 25. doi: 10.1107/S1744309110053819
PMCID:
PMC3053162
Jason van Rooyen, Hassan Belrhali, Valarie Abratt, B. Trevor Sewell

This work demonstrates how the digestion of a surface-exposed loop of B. fragilis type III glutamine synthetase results in crystal-packing rearrangements and explains how these changes facilitated the first structure solution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 358–363. Published online 2011 February 25. doi: 10.1107/S1744309110053893
PMCID:
PMC3053163
Cristina Yunta, Martin Martinez-Ripoll, Armando Albert

Serine/threonine protein kinase SnRK2.6/OST1 (OPEN STOMATA 1) from A. thaliana has been crystallized by the hanging-drop vapour-diffusion method and a native data set has been collected to 2.8 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 364–368. Published online 2011 February 25. doi: 10.1107/S1744309110053807
PMCID:
PMC3053164
Jessica Huyet, Maryse Gilbert, Michel R. Popoff, Ajit Basak

The crystallization of the C. perfringens delta-toxin is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 369–371. Published online 2011 February 25. doi: 10.1107/S1744309110054187
PMCID:
PMC3053165
Agnieszka J. Pietrzyk, Anna Bujacz, Małgorzata Łochyńska, Mariusz Jaskólski, Grzegorz Bujacz

Juvenile hormone-binding protein and PBMHP-12, two major 30 kDa proteins, have been isolated and purified from the haemolymph of B. mori. The proteins were crystallized and the crystals diffracted X-rays to 2.9 and 1.3 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 372–376. Published online 2011 February 25. doi: 10.1107/S1744309110054564
PMCID:
PMC3053166
Alexandra Müller, Christine Schlicker, Maria Fehringer, Bernd Masepohl, Eckhard Hofmann

The DNA-binding domain of R. capsulatus MopB has been purified and crystallized for X-ray structure analysis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 377–379. Published online 2011 February 25. doi: 10.1107/S1744309110054710
PMCID:
PMC3053167
Linda Schuldt, Ruth Suchowersky, Katharina Veith, Jochen Mueller-Dieckmann, Manfred S. Weiss

The regulatory domain of M. tuberculosis aspartokinase, the enzyme which catalyses the first reaction step in the biosynthesis of the amino acids lysine, methionine and threonine, has been cloned, expressed, purified and crystallized. Preliminary X-ray diffraction analysis of several crystals revealed the presence of five distinct crystal forms.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 380–385. Published online 2011 February 25. doi: 10.1107/S1744309111000030
PMCID:
PMC3053168
Valentina Zorzini, Sarah Haesaerts, Niles P. Donegan, Zhibiao Fu, Ambrose L. Cheung, Nico A. J. van Nuland, Remy Loris

Crystals of S. aureus MazF belong to space group P212121, diffract to 2.1 Å resolution and contain two MazF dimers in the asymmetric unit.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 386–389. Published online 2011 February 25. doi: 10.1107/S1744309111000571
PMCID:
PMC3053169
Susana Gonçalves, Ana M. Esteves, Nuno Borges, Helena Santos, Pedro M. Matias

The cloning, expression, purification, crystallization and preliminary crystallographic analysis of mannosyl-3-phosphoglycerate phosphatase (MpgP) from T. thermophilus HB27 are reported. The stability of MpgP in solution was studied by size-exclusion chromatography and differential scanning fluorimetry assays.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 390–396. Published online 2011 February 25. doi: 10.1107/S1744309111002843
PMCID:
PMC3053170
Maryna Lahoda, Radka Chaloupkova, Alena Stsiapanava, Jiri Damborsky, Ivana Kuta Smatanova

A mutant of the haloalkane dehalogenase DhaA (DhaA31) from R. rhodochrous NCIMB 13064 and its complex with 1,2,3-trichloropropane were crystallized and the crystals diffracted to high resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 397–400. Published online 2011 February 25. doi: 10.1107/S1744309111001254
PMCID:
PMC3053171
Mohd Saif Khusaini, Raja Noor Zaliha Raja Abd. Rahman, Mohd Shukuri Mohamad Ali, Thean Chor Leow, Mahiran Basri, Abu Bakar Salleh

Crystallographic data of organic solvent-tolerant lipase from Bacillus sp. strain 42 was collected at 2.0 Å with unit-cell parameters a =117.41, b = 80.85, c = 99.44 Å, β=96.40°. The protein–solvent interactions will be studied since lipase 42 was stable in water-miscible solvent.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 401–403. Published online 2011 February 25. doi: 10.1107/S1744309111002028
PMCID:
PMC3053172
Claire Debarnot, Isabelle Imbert, François Ferron, Laure Gluais, Isabelle Varlet, Nicolas Papageorgiou, Mickaël Bouvet, Julien Lescar, Etienne Decroly, Bruno Canard

The expression, purification and crystallization of the SARS coronavirus nsp16 RNA-cap AdoMet-dependent (nucleoside-2′O)-methyltransferase in complex with its activating factor nsp10 are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 404–408. Published online 2011 February 25. doi: 10.1107/S1744309111002867
PMCID:
PMC3053173
Hiroyuki Morita, Takahiro Mori, Kiyofumi Wanibuchi, Ryohei Kato, Shigetoshi Sugio, Ikuro Abe

4-Coumarate:CoA ligase 2 from A. thaliana has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to a resolution of 1.6 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 409–411. Published online 2011 February 25. doi: 10.1107/S1744309111002181
PMCID:
PMC3053174
Sean Jackson, Seiji Sugiman-Marangos, Kelvin Cheung, Murray Junop

A truncated version of pleckstrin encompassing the amino-terminal PH domain and the central DEP domain has been crystallized and preliminary diffraction analysis has been conducted. The quaternary structure and phospholipid-binding properties of this pleckstrin truncation were analyzed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 412–416. Published online 2011 February 25. doi: 10.1107/S174430911005092X
PMCID:
PMC3053175
Orapin Ariyawutthiphan, Toyoyuki Ose, Muneya Tsuda, YongGui Gao, Min Yao, Atsushi Minami, Hideaki Oikawa, Isao Tanaka

Crystals of a geranyl pyrophosphate methyltransferase in the biosynthetic pathway of the off-flavor terpenoid alcohol, 2-methylisoborneol were obtained in the absence and presence of cofactor, cofactor analog and substrate.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 417–420. Published online 2011 February 25. doi: 10.1107/S1744309110051523
PMCID:
PMC3053176

Laboratory Communications

Xenia Bogdanović, Winfried Hinrichs

The effect of temperature during crystallization setup was analyzed and was found to influence precipitate formation and the crystal shape of the inactive E294A mutant of A. salmonicida ssp. achromogenes protease 1.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 March 1; 67(Pt 3): 421–423. Published online 2011 February 25. doi: 10.1107/S1744309111001783
PMCID:
PMC3053177

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography