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This journal provides early access to some articles.
Volume 66(Pt 9);  September 1, 2010
Structural Communications
Justyna E. Korczynska, Steffen Danielsen, Ulrika Schagerlöf, Johan P. Turkenburg, Gideon J. Davies, Keith S. Wilson, Edward J. Taylor
The X-ray structure of a fungal lysozyme shows a modified α/β-barrel-like fold and a likely active-site DXE motif.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 973–977. Published online Aug 21, 2010. doi: 10.1107/S1744309110025601
PMCID:
PMC2935209
Benjamin Schumacher, Malgorzata Skwarczynska, Rolf Rose, Christian Ottmann
The first structure of a 14-3-3 protein–phosphopeptide complex is reported at 1.15 Å resolution. The YAP 14-3-3-binding motif is revealed for the first time using crystallographic tools.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 978–984. Published online Aug 21, 2010. doi: 10.1107/S1744309110025479
PMCID:
PMC2935210
Jingzhi Li, Wenjun Cui, Bingdong Sha
Tom70 family members may exhibit structural plasticity when complexed with Hsp70/Hsp90. This structural plasticity enables Tom70 to accommodate various precursor substrates for mitochondrial translocation.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 985–989. Published online Aug 21, 2010. doi: 10.1107/S1744309110025522
PMCID:
PMC2935211
M. Pal, P. T. Erskine, R. S. Gill, S. P. Wood, J. B. Cooper
The type III secretion system needle-tip protein BipD has been crystallized in a form that diffracts X-rays to 1.5 Å resolution and the structure has been refined to an R factor of 16.1% and an R free of 19.8% at this resolution. The putative antiparallel dimer interface that was observed in earlier structures is conserved.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 990–993. Published online Aug 21, 2010. doi: 10.1107/S1744309110026333
PMCID:
PMC2935212
Jean-François Trempe, Nicholas R. Brown, Martin E. M. Noble, Jane A. Endicott
A new crystal form of Lys48-linked diubiquitin was obtained and its structure was determined by X-ray crystallography to 1.6 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 994–998. Published online Aug 21, 2010. doi: 10.1107/S1744309110027600
PMCID:
PMC2935213
Michael J. Rudolph, Gabriele A. Amodeo, Liang Tong
An inhibited conformation of the protein kinase domain (KD) of Snf1 is observed. The auto-inhibitory domain (AID) is disordered.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 999–1002. Published online Aug 21, 2010. doi: 10.1107/S1744309110028265
PMCID:
PMC2935214
Stephen J. Tomanicek, Ronny C. Hughes, Joseph D. Ng, Leighton Coates
When compared with their E. coli homologue, two X-ray crystal structures of T. maritima endonuclease IV differing in the composition of the trinuclear metal site point to the importance of the trinuclear site and its modulation among species to the function of this enzyme in the AP endonuclease IV family.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1003–1012. Published online Aug 21, 2010. doi: 10.1107/S1744309110028575
PMCID:
PMC2935215
Adriana Irimia, Lioudmila V. Loukachevitch, Robert L. Eoff, F. Peter Guengerich, Martin Egli
Structures of binary and ternary Dpo4 DNA polymerase complexes crystallized in the presence of Mg2+ have been determined. Comparisons with the corresponding Ca2+-form structures revealed subtle changes in the active-site geometry.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1013–1018. Published online Aug 21, 2010. doi: 10.1107/S1744309110029374
PMCID:
PMC2935216
Daniela De Luchi, Isabel Usón, Glenford Wright, Catherine Gouyette, Juan A. Subirana
The molecular structure of a complex of an anthraquinone derivative and the oligonucleotide d(UBrAGG) is presented. The anthraquinone molecules are stacked. Isolated base pairs are intercalated in the stack of drug molecules.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1019–1022. Published online Aug 21, 2010. doi: 10.1107/S1744309110030034
PMCID:
PMC2935217
Ana Cámara-Artigas, Monserrat Andújar-Sánchez, Emilia Ortiz-Salmerón, Celia Cuadri, Eva S. Cobos, Jose Manuel Martin-Garcia
The structure of an α-spectrin SH3-domain mutant with a stabilized hydrophobic core and distal loop has been solved at 1 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1023–1027. Published online Aug 21, 2010. doi: 10.1107/S1744309110030095
PMCID:
PMC2935218
Koen Robeyns, Piet Herdewijn, Luc Van Meervelt
The crystal structure of the tetragonal form of the non-self-complementary DNA sequence d[GCG(xT)GCG]/d(CGCACGC) with one incorporated CeNA (xT) moiety has been solved and is compared with the high-resolution orthorhombic form.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1028–1031. Published online Aug 26, 2010. doi: 10.1107/S1744309110031696
PMCID:
PMC2935219
Michael Koch, Joachim Diez, Armin Wagner, Günter Fritz
The structure of Ca2+-bound EF-hand protein S100A2 was determined by calcium and sulfur SAD at a wavelength of 0.90 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1032–1036. Published online Aug 26, 2010. doi: 10.1107/S1744309110030691
PMCID:
PMC2935220
Crystallization Communications
Thyageshwar Chandran, Alok Sharma, M. Vijayan
A galactose-specific lectin purified from the seeds of bitter gourd (M. charantia) has been crystallized and preliminary X-ray study of the crystals has been carried out.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1037–1040. Published online Aug 26, 2010. doi: 10.1107/S174430911002659X
PMCID:
PMC2935221
Francieli Colussi, Larissa C. Textor, Viviane Serpa, Roberto Nobuyuki Maeda, Nei Pereira, Jr, Igor Polikarpov
The catalytic domain of CHBI was purified from a cellular extract of T. harzianum. Diffraction-quality crystals were obtained and a native X-ray data set was collected using a synchrotron source.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1041–1044. Published online Aug 26, 2010. doi: 10.1107/S1744309110026886
PMCID:
PMC2935222
Andrey M. Grishin, Eunice Ajamian, Linhua Zhang, Miroslaw Cygler
The expression, purification, crystallization and preliminary crystallographic analysis of the PaaAC complex is reported. This is the main component of the E. coliphenylacetyl-coenzyme A oxygenase complex.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1045–1049. Published online Aug 26, 2010. doi: 10.1107/S174430911002748X
PMCID:
PMC2935223
Kavitha Marapakala, A. Abdul Ajees, Jie Qin, Banumathi Sankaran, Barry P. Rosen
A common biotransformation of arsenic is methylation to monomethylated, dimethylated and trimethylated species, which is catalyzed by the ArsM (or AS3MT) arsenic(III) S-adenosylmethionine methyltransferase. ArsM from the acidothermophilic alga Cyanidioschyzon sp. 5508 was expressed, purified and crystallized by the hanging-drop vapor-diffusion method and diffraction data were collected to 1.76 Å
...
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1050–1052. Published online Aug 26, 2010. doi: 10.1107/S1744309110027661
PMCID:
PMC2935224
Qianda Lu, Jinming Ma, Hui Rong, Jun Fan, Ye Yuan, Kuai Li, Yongxiang Gao, Xiao Zhang, Maikun Teng, Liwen Niu
Crystals of 5-aminolaevulinic acid dehydratase from B. subtilis are tetragonal, belonging to space group P42212, and diffract to 2.7 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1053–1055. Published online Aug 26, 2010. doi: 10.1107/S1744309110027582
PMCID:
PMC2935225
Ricardo Adaixo, João Henrique Morais-Cabral
The N-terminal PAS domains from the eukaryotic EAG potassium channels are thought to have a regulatory function. Here the expression, purification, crystallization and preliminary crystallographic characterization of two of these domains are described.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1056–1059. Published online Aug 26, 2010. doi: 10.1107/S1744309110027880
PMCID:
PMC2935226
Breann L. Brown, Rebecca Page
The E. coli antitoxin MqsA (YgiT/b3021) has been cocrystallized with mqsRA promoter DNA. A native data set has been collected to a resolution of 2.1 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1060–1063. Published online Aug 26, 2010. doi: 10.1107/S1744309110028617
PMCID:
PMC2935227
Yoshifumi Maeda, Daiju Doubayashi, Takumi Ootake, Masaya Oki, Bunzo Mikami, Hiroyuki Uchida
Formate oxidase from A. oryzae RIB40 was crystallized and diffraction data were collected to a resolution of 2.4 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1064–1066. Published online Aug 26, 2010. doi: 10.1107/S1744309110028605
PMCID:
PMC2935228
Kazuki Kawahara, Shota Nakamura, Yasuhiro Katsu, Daisuke Motooka, Yuki Hosokawa, Yukiko Kojima, Keiko Matsukawa, Hiroto Takinowaki, Susumu Uchiyama, Yuji Kobayashi, Kiichi Fukui, Tadayasu Ohkubo
In this study, the human condensin SMC2 hinge domain with short coiled coils was cloned, expressed, purified and crystallized in the orthorhombic space group C222 in native and SeMet-derivatized forms.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1067–1070. Published online Aug 26, 2010. doi: 10.1107/S1744309110028721
PMCID:
PMC2935229
Cerrone Cabanos, Hiroyuki Urabe, Taro Masuda, Mary Rose Tandang-Silvas, Shigeru Utsumi, Bunzo Mikami, Nobuyuki Maruyama
In this study, the core region of Ara h 1, one of the major peanut allergens, has been overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.25 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1071–1073. Published online Aug 26, 2010. doi: 10.1107/S1744309110029040
PMCID:
PMC2935230
Javier García-Nafría, Meike Baumgart, Michael Bott, Anthony J. Wilkinson, Keith S. Wilson
Crystallization of AcnR, a repressor of the aconitase gene in Corynebacterium glutamicum, is reported. Intentional manual scratching of the crystallization plates was applied to induce heterogeneous nucleation.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1074–1077. Published online Aug 26, 2010. doi: 10.1107/S1744309110029015
PMCID:
PMC2935231
Camila Ramos Santos, Fabio Marcio Squina, Andreia Meza Navarro, Roberto Ruller, Rolf Prade, Mario Tyago Murakami
The catalytic domain of a hyperthermostable endo-1,4-β-d-mannanase from T. petrophila RKU-1 has been cloned, overexpressed in E. coli cells, purified and crystallized in two distinct crystalline forms by the sitting-drop vapour-diffusion method.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1078–1081. Published online Aug 26, 2010. doi: 10.1107/S1744309110029131
PMCID:
PMC2935232
Kaoru Suzuki, Satoru Shimizu, Ella Czarina Magat Juan, Takahiro Miyamoto, Zhang Fang, Md. Mominul Hoque, Yoshiteru Sato, Masaru Tsunoda, Takeshi Sekiguchi, Akio Takénaka, Shi-Yuan Yang
The carbonic anhydrase αCA1 from C. reinhardtii is a dimeric class αCA enzyme with post-translational glycosylation at three asparagine residues and proteolytic removal of a short peptide. The mature enzyme has been crystallized, MAD data have been collected to 1.88 Å resolution and a preliminary solution of the crystal structure has been obtained.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1082–1085. Published online Aug 28, 2010. doi: 10.1107/S174430911002823X
PMCID:
PMC2935233
Keisuke Ito, Sohei Ito, Tatsuro Shimamura, Yasuaki Kawarasaki, Keiko Abe, Takumi Misaka, Takuya Kobayashi, So Iwata
In this study, the glucansucrase from the dental caries pathogen S. mutans was purified and crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1086–1088. Published online Aug 28, 2010. doi: 10.1107/S1744309110029714
PMCID:
PMC2935234
Sachin Wakadkar, Li-Qing Zhang, Duo-Chuan Li, Teemu Haikarainen, Prathusha Dhavala, Anastassios C. Papageorgiou
Cu,Zn superoxide dismutase from the thermophilic fungus C. thermophilum was expressed in P. pastoris, purified and crystallized. A complete data set was collected to 1.9 Å resolution using synchrotron radiation.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1089–1092. Published online Aug 28, 2010. doi: 10.1107/S1744309110030393
PMCID:
PMC2935235
Anu V. Chandran, J. Rajan Prabu, G. P. Manjunath, K. Neelakanteshwar Patil, K. Muniyappa, M. Vijayan
The C-terminal domain of M. tuberculosis LexA has been crystallized and its structure has been solved.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1093–1095. Published online Aug 28, 2010. doi: 10.1107/S174430911003068X
PMCID:
PMC2935236
Baldeep Khare, Alexandra Samal, Krishnan Vengadesan, K. R. Rajashankar, Xin Ma, I-Hsiu Huang, Hung Ton-That, Sthanam V. L. Narayana
The expression, purification, crystallization and preliminary X-ray diffraction analysis of pili-specific and housekeeping sortases from S. agalactiae are reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1096–1100. Published online Aug 28, 2010. doi: 10.1107/S1744309110031106
PMCID:
PMC2935237
Mor Sendovski, Margarita Kanteev, Vered Shuster Ben-Yosef, Noam Adir, Ayelet Fishman
The crystallization and primary crystallographic analysis of B. megaterium tyrosinase is reported. The purified protein was crystallized in the absence or presence of zinc ions to a resolution of 2.0 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1101–1103. Published online Aug 28, 2010. doi: 10.1107/S1744309110031520
PMCID:
PMC2935238
Carl A. Morrow, Anna Stamp, Eugene Valkov, Bostjan Kobe, James A. Fraser
IMP dehydrogenase from C. neoformans and C. gattii has been crystallized in complex with the substrate IMP and the cofactor NAD+.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1104–1107. Published online Aug 28, 2010. doi: 10.1107/S1744309110031659
PMCID:
PMC2935239
Janice A. Frias, Brandon R. Goblirsch, Lawrence P. Wackett, Carrie M. Wilmot
OleC crystals belonged to space group P3121 or P3221 and diffracted to 3.4 Å resolution at a third-generation synchrotron X-ray source.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1108–1110. Published online Aug 28, 2010. doi: 10.1107/S1744309110031751
PMCID:
PMC2935240
Juke Wang, Tong-Cun Zhang, Xinqi Liu
The preliminary crystallographic analysis of the N-terminal domain of FILIA is described in this paper. FILIA is a component of subcortical maternal complex, which plays critical roles in embryogenesis.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1111–1114. Published online Aug 28, 2010. doi: 10.1107/S1744309110031994
PMCID:
PMC2935241
Tomomi Sumida, Tatsuo Yanagisawa, Ryohei Ishii, Shigeyuki Yokoyama
GenX, a lysyl-tRNA synthetase paralogue from Escherichia coli, has been overexpressed in E. coli, purified by three chromatographic steps and cocrystallized with a lysyl adenylate analogue (LysAMS) by the hanging-drop vapour-diffusion method using PEG 4000 as a precipitant.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1115–1118. Published online Aug 28, 2010. doi: 10.1107/S1744309110032008
PMCID:
PMC2935242
Ekaterina Sviridova, Ladislav Bumba, Pavlina Rezacova, Katerina Prochazkova, Daniel Kavan, Karel Bezouska, Michal Kuty, Peter Sebo, Ivana Kuta Smatanova
Native FrpD43–271 and selenomethionine-derivative FrpD43–271 proteins were purified, crystallized and diffracted to resolutions of 2.25 and 2.00 Å, respectively.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1119–1123. Published online Aug 28, 2010. doi: 10.1107/S174430911003215X
PMCID:
PMC2935243
Naoyuki Kuwabara, Hiroshi Hashimoto, Noriyo Yamada, Satoru Unzai, Mitsunori Ikeguchi, Mamoru Sato, Yasuto Murayama, Hiroshi Iwasaki, Toshiyuki Shimizu
The crystallization of the homologous recombination mediators Swi5 and Sfr1 from fission yeast is reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1124–1126. Published online Aug 31, 2010. doi: 10.1107/S1744309110032239
PMCID:
PMC2935244
Jacqueline E. Day, Troii Hall, Lyle E. Pegg, Timothy E. Benson, Jens Hausmann, Satwik Kamtekar
Autotaxin (ATX), a pyrophosphatase/phosphodiesterase enzyme, is a promising drug target for many indications and is only distantly related to enzymes of previously determined structure. Here, the cloning, expression, purification, crystallization and preliminary diffraction analysis of ATX are reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1127–1129. Published online Aug 31, 2010. doi: 10.1107/S1744309110030228
PMCID:
PMC2935245
Jens Hausmann, Evangelos Christodoulou, Mobien Kasiem, Valeria De Marco, Laurens A. van Meeteren, Wouter H. Moolenaar, Danny Axford, Robin L. Owen, Gwyndaf Evans, Anastassis Perrakis
Autotaxin, a four-domain ~100 kDa mammalian glycoprotein, was expressed in stably transfected mammalian cells, purified from the medium and crystallized. Diffraction data from micrometre-thick crystal plates were collected on various European synchrotron beamlines and are presented and analysed.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Sep 1, 2010; 66(Pt 9): 1130–1135. Published online Aug 31, 2010. doi: 10.1107/S1744309110032938
PMCID:
PMC2935246
Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of
International Union of Crystallography