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Volume 66(Pt 7);  July 1, 2010

Structural Communications

Somporn Katekaew, Buabarn Kuaprasert, Takao Torikata, Yoshimitsu Kakuta, Makoto Kimura, Kazunari Yoneda, Tomohiro Araki

The structure of GTRNase has been solved at 1.60 Å resolution by the molecular-replacement technique.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 755–759. Published online Jun 23, 2010. doi: 10.1107/S1744309110016751
PMCID:
PMC2898455
Olga Moskaleva, Bogdan Melnik, Azat Gabdulkhakov, Maria Garber, Stanislav Nikonov, Elena Stolboushkina, Alexei Nikulin

The results obtained demonstrate the great importance of solvent-inaccessible conserved hydrogen bonds between the Hfq monomers in the stabilization of the hexamer structure.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 760–764. Published online Jun 23, 2010. doi: 10.1107/S1744309110017331
PMCID:
PMC2898456
Kimihiko Mizutani, Mayuko Toyoda, Kenta Sagara, Nobuyuki Takahashi, Atsuko Sato, Yuji Kamitaka, Seiya Tsujimura, Yuji Nakanishi, Toshiyuki Sugiura, Shotaro Yamaguchi, Kenji Kano, Bunzo Mikami

The crystal structure of bilirubin oxidase (BOD) from M. verrucaria has been determined at 2.3 Å resolution using a merohedrally twinned crystal. BOD has four copper-coordination sites that are almost identical to those of other multicopper oxidases and is also very similar to them in overall structure.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 765–770. Published online Jun 23, 2010. doi: 10.1107/S1744309110018828
PMCID:
PMC2898457
Tian-Min Fu, Xiang Liu, Lanfen Li, Xiao-Dong Su

The crystal structure of smu.1377c, a hypothetical protein from S. mutans, shows a similar fold to Sua5_YciO_YrdC-family proteins and indicates its functional role in tRNA modification.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 771–775. Published online Jun 23, 2010. doi: 10.1107/S1744309110018944
PMCID:
PMC2898458
Ikechukwu Achilonu, Samantha Gildenhuys, Loren Fisher, Jonathan Burke, Sylvia Fanucchi, B. Trevor Sewell, Manuel Fernandes, Heini W. Dirr

The role of a topologically conserved isoleucine in the structure of glutathione transferase was investigated by replacing the Ile71 residue in human GSTA1-1 by alanine or valine.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 776–780. Published online Jun 23, 2010. doi: 10.1107/S1744309110019135
PMCID:
PMC2898459
Jeff E. Habel, Evan H. Bursey, Beom-Seop Rho, Chang-Yub Kim, Brent W. Segelke, Bernhard Rupp, Min S. Park, Thomas C. Terwilliger, Li-Wei Hung

Crystal and solution structures of Rv1848 protein and their implications in the biological assembly of Mtb urease is presented.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 781–786. Published online Jun 23, 2010. doi: 10.1107/S1744309110019536
PMCID:
PMC2898460
Chen Hu, Xuan Zhang, Yan-Bin Teng, Hai-Xi Hu, Wei-Fang Li

The crystal structure of autophagy-related protein Atg8 from the silkworm B. mori has two additional helices at the N-terminus before the expected ubiquitin fold.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 787–790. Published online Jun 23, 2010. doi: 10.1107/S1744309110018464
PMCID:
PMC2898461

Crystallization Communications

Hideaki Morioka, Yasuhiro Miki, Kei Saito, Koji Tomoo, Toshimasa Ishida, Tomokazu Hasegawa, Akihito Yamano, Chiaki Takada, Katsushiro Miyamoto, Hiroshi Tsujibo

BxlA from Streptomyces thermoviolaceus OPC-520 (molecular weight 82 kDa) was crystallized by the hanging-drop vapour-diffusion method at 289 K.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 791–793. Published online Jun 24, 2010. doi: 10.1107/S1744309110013400
PMCID:
PMC2898462
David C. Briggs, James G. Smedley, III, Bruce A. McClane, Ajit K. Basak

The crystallization of the C. perfringens enterotoxin is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 794–797. Published online Jun 24, 2010. doi: 10.1107/S1744309110016507
PMCID:
PMC2898463
Pascal Retailleau, Alexander A. Ishchenko, Nikita A. Kuznetsov, Murat Saparbaev, Solange Moréra

2.2 and 2.7 Å resolution data sets were collected from crystals of the human apurinic/apyrimidinic endonuclease 1 (APE1) truncated of the first 61 N-terminal residues in the presence of a 15-mer DNA containing an oxidatively damaged base as the target base. For both complexes, APE1 crystallized with a 6-mer DNA, suggesting that nucleotide incision and 3′→5′ exonuclease

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 798–800. Published online Jun 24, 2010. doi: 10.1107/S1744309110017021
PMCID:
PMC2898464
Mayuko Toyoda, Keiji Jitsumori, Bunzo Mikami, Lawrence P. Wackett, Tatsuo Kurihara, Nobuyoshi Esaki

l-Azetidine-2-carboxylate hydrolase from Pseudomonas sp. strain A2C was crystallized and diffraction data were collected to a resolution of 1.38 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 801–804. Published online Jun 24, 2010. doi: 10.1107/S1744309110017045
PMCID:
PMC2898465
Georgiana Petrareanu, Mihaela C. Balasu, Ulrich Zander, Axel J. Scheidig, Stefan E. Szedlacsek

The expression, purification, preliminary crystallization and crystallographic analysis of phosphoketolase from L. lactis ssp. lactis (strain IL 1403) are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 805–807. Published online Jun 24, 2010. doi: 10.1107/S174430911001732X
PMCID:
PMC2898466
Anandhi Anandan, Corinne Vallet, Travis Coyle, Ibrahim M. Moustafa, Alice Vrielink

Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 808–810. Published online Jun 24, 2010. doi: 10.1107/S1744309110017185
PMCID:
PMC2898467
Timothy D. Panosian, David P. Nannemann, Brian O. Bachmann, T. M. Iverson

Two crystal forms of an Mn2+-dependent phosphopentomutase were identified from chemically distinct conditions by sparse-matrix screening with and without the inclusion of 50 mM Mn2+. The crystals identified in the presence of Mn2+ were of dramatically better diffraction quality than those identified in the absence of added Mn2+.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 811–814. Published online Jun 24, 2010. doi: 10.1107/S1744309110017549
PMCID:
PMC2898468
I-Jung Chen, Chia-Cheng Chou, Chia-Ling Liu, Cheng-Chung Lee, Lou-Sing Kan, Ming-Hon Hou

Crystals of the N-terminal domain of nucleocapsid protein from human coronavirus OC43 were obtained that diffracted X-rays to a resolution of at least 1.7 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 815–818. Published online Jun 24, 2010. doi: 10.1107/S1744309110017616
PMCID:
PMC2898469
Rafael Miguez Couñago, Stephen B. Fleming, Andrew A. Mercer, Kurt L. Krause

The chemokine-binding protein from orf virus was purified and crystallized. The morphology and diffraction behaviour of these crystals was significantly improved through the use of additives known as Silver Bullets.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 819–823. Published online Jun 24, 2010. doi: 10.1107/S1744309110018166
PMCID:
PMC2898470
Natalie Zeytuni, Raz Zarivach

Here, the crystallization and preliminary X-ray analysis of recombinant and purified Magnetospirillum magneticum and M. gryphiswaldense MamA are reported for the first time.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 824–827. Published online Jun 24, 2010. doi: 10.1107/S1744309110018300
PMCID:
PMC2898471
Xenia Bogdanović, Martin Hesseler, Gottfried J. Palm, Uwe T. Bornscheuer, Winfried Hinrichs

The crystallization and preliminary X-ray diffraction studies of DppA from P. pacifica SIR-I are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 828–830. Published online Jun 24, 2010. doi: 10.1107/S1744309110018932
PMCID:
PMC2898472
Carla Protsko, Claire Vieille, Maris Laivenieks, Lata Prasad, David A. R. Sanders, Louis T. J. Delbaere

The secondary alcohol dehydrogenase mutant I86A from Thermoanaerobacter ethanolicus (TeSADH) was crystallized in novel crystallization conditions. Diffraction data to 3.2 Å were collected at the Canadian Light Source.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 831–833. Published online Jun 24, 2010. doi: 10.1107/S1744309110018981
PMCID:
PMC2898473
Daichi Morimoto, Shin Isogai, Takeshi Tenno, Hidehito Tochio, Masahiro Shirakawa, Mariko Ariyoshi

Lys48-linked tetraubiquitin, hexaubiquitin and octaubiquitin were enzymatically synthesized, purified and crystallized. X-ray diffraction data sets for tetraubiquitin and hexaubiquitin were collected at 1.6 and 1.8 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 834–837. Published online Jun 24, 2010. doi: 10.1107/S1744309110018804
PMCID:
PMC2898474
Klaus Breicha, Marion Müller, Werner Hummel, Karsten Niefind

The alcohol dehydrogenase Gre2p from S. cerevisiae catalyses the stereospecific reduction of a variety of different keto compounds and can therefore be applied as a valuable biocatalyst. The crystallization of the complex of Gre2p with NADP+ and its preliminary X-ray analysis are described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 838–841. Published online Jun 24, 2010. doi: 10.1107/S1744309110018889
PMCID:
PMC2898475
Asako Kounosu, Kazuya Hasegawa, Toshio Iwasaki, Takashi Kumasaka

A hyperthermophilic archaeal Rieske-type [2Fe–2S] ferredoxin (ARF) from S. solfataricus P1 has been crystallized as a recombinant protein with a vector-derived long N-terminal extension region. The P43212 crystals of recombinant ARF diffracted to 1.85 Å resolution using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 842–845. Published online Jun 24, 2010. doi: 10.1107/S1744309110019263
PMCID:
PMC2898476
Sachiko Takahashi, Toshiharu Tsurumura, Kosuke Aritake, Naoki Furubayashi, Masaru Sato, Mari Yamanaka, Erika Hirota, Satoshi Sano, Tomoyuki Kobayashi, Tetsuo Tanaka, Koji Inaka, Hiroaki Tanaka, Yoshihiro Urade

High-quality crystals of human haematopoietic prostaglandin D synthase in complex with novel inhibitors were obtained in microgravity.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 846–850. Published online Jun 24, 2010. doi: 10.1107/S1744309110020828
PMCID:
PMC2898477
Zhongyuan Liu, Qiangjun Zhou, Xinfang Mao, Xiangdong Zheng, Jiubiao Guo, Fuchun Zhang, Tingyi Wen, Hai Pang

Cecropin B derived from the hemolymph of Bombyx mori has been crystallized by the hanging-drop vapour-diffusion method. The crystal diffracted to 1.43 Å resolution using X-ray radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 851–853. Published online Jun 24, 2010. doi: 10.1107/S1744309110020130
PMCID:
PMC2898478
Lei Jin, Chungen Pan, Zhi Qi, Z. Hong Zhou, Shibo Jiang

The Fab fragment of NC-1, a murine antibody that specifically recognizes the six-helix bundle core of HIV-1 gp41, has been crystallized in space group P3221. An X-ray diffraction data set was collected at 3.2 Å resolution and a clear molecular-replacement solution was obtained for solution of the structure.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 854–857. Published online Jun 24, 2010. doi: 10.1107/S1744309110019287
PMCID:
PMC2898479
Hua Xiang, Fangfang Gao, Dacheng Wang, Jing Liu, Jia Hu, Liqing Zhang, Shentao Li, Xuming Deng

The adhesive domain of SdrE from Staphylococcus aureus was recombinantly expressed in Escherichia coli and crystallized. X-ray diffraction data were collected to 1.8 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 858–861. Published online Jun 24, 2010. doi: 10.1107/S1744309110020907
PMCID:
PMC2898480
J. A. Gómez Barroso, H. Pereira, M. Miranda, C. Pereira, R. C. Garratt, C. F. Aguilar

T. cruzi TcNDPK1 was overexpressed in Escherichia coli as an N-­terminally poly-His-tagged fusion protein and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2010; 66(Pt 7): 862–865. Published online Jun 26, 2010. doi: 10.1107/S1744309110013886
PMCID:
PMC2898481

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography