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Volume 66(Pt 3);  2010 March 1

Structural Communications

Hugh P. Morgan, Iain W. McNae, Kun-Yi Hsin, Paul A. M. Michels, Linda A. Fothergill-Gilmore, Malcolm D. Walkinshaw

The first crystal structure of Leishmania mexicana pyruvate kinase (LmPYK) obtained at a neutral pH. LmPYK was co-crystallized with the small molecule 1,3,6,8-pyrenetetrasulfonic acid, which provides a helpful intermolecular bridge between macromolecules.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 215–218. Published online 2010 February 23. doi: 10.1107/S1744309109053494
PMCID:
PMC2833022
David R. Cooper, Katarzyna Grelewska, Chang-Yub Kim, Andrzej Joachimiak, Zygmunt S. Derewenda

The structure of DinB from G. stearothermophilus is described and compared with a number of recently reported structures of this unusual fold. Structural similarities are revealed that unite several distant protein families.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 219–224. Published online 2010 February 23. doi: 10.1107/S1744309109053913
PMCID:
PMC2833023
Michèle Salem, Yves Mauguen, Thierry Prangé

A specific Lys–Arg cross-link is observed in trimeric barnase crystals.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 225–228. Published online 2010 February 23. doi: 10.1107/S1744309109054037
PMCID:
PMC2833024
Alexey Teplyakov, Galina Obmolova, Alison Rogers, Gary L. Gilliland

The X-ray structure of the anti-IL-23 antibody CNTO4088 reveals an unusual conformation of CDR L1 despite the fact that the residues in key positions fit the pattern of the canonical structure.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 229–232. Published online 2010 February 23. doi: 10.1107/S1744309109054141
PMCID:
PMC2833025
Snezana Djordjevic, Xiaoxuan Zhang, Mark Bartlam, Sheng Ye, Zihe Rao, Christopher J. Danpure

The crystal structure of the G170R mutant form of human alanine:glyoxylate aminotransferase has been determined at 2.6 Å resolution. This mutation is associated with enzyme mistargeting in the hereditary kidney-stone disease primary hyperoxaluria type 1.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 233–236. Published online 2010 February 23. doi: 10.1107/S1744309109054645
PMCID:
PMC2833026
Justyna Kalinowska-Tłuścik, Linda Miallau, Mads Gabrielsen, Gordon A. Leonard, Sean M. McSweeney, William N. Hunter

The structure of a triclinic crystal form of 4-diphosphocytidyl-2C-methyl-d-erythritol kinase has been determined. Comparisons with a previously reported monoclinic crystal form raise questions about our knowledge of the quaternary structure of this enzyme.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 237–241. Published online 2010 February 23. doi: 10.1107/S1744309109054591
PMCID:
PMC2833027
Sum Chan, Iulia Giuroiu, Irina Chernishof, Michael R. Sawaya, Janet Chiang, Robert P. Gunsalus, Mark A. Arbing, L. Jeanne Perry

Crystal structures of ModA from M. acetivorans in the apo and ligand-bound conformations confirm domain rotation upon ligand binding.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 242–250. Published online 2010 February 23. doi: 10.1107/S1744309109055158
PMCID:
PMC2833028
Kazuhiro Ohta, Kazumasa Muramoto, Kyoko Shinzawa-Itoh, Eiki Yamashita, Shinya Yoshikawa, Tomitake Tsukihara

The structure of nitric oxide-treated bovine heart cytochrome c oxidase (CcO) in the fully reduced state was determined at 50 K under light illumination.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 251–253. Published online 2010 February 23. doi: 10.1107/S1744309109055109
PMCID:
PMC2833029
Honghua Ge, Yongxiang Gao, Yuzhi Hong, Min Zhang, Yazhong Xiao, Maikun Teng, Liwen Niu

The crystal structure of a fully active laccase from Trametes sp. AH28-2 is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 254–258. Published online 2010 February 23. doi: 10.1107/S1744309110000084
PMCID:
PMC2833030
V. I. Timofeev, R. N. Chuprov-Netochin, V. R. Samigina, V. V. Bezuglov, K. A. Miroshnikov, I. P. Kuranova

Crystals of insulin have been grown from a solution containing insulin and polysialic acid and the three-dimensional structure of insulin in these crystals has been solved at 1.6 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 259–263. Published online 2010 February 23. doi: 10.1107/S1744309110000461
PMCID:
PMC2833031

Crystallization Communications

Carlos A. Nieves-Marrero, Carlos R. Ruiz-Martínez, Rafael A. Estremera-Andújar, Luis A. González-Ramírez, Juan López-Garriga, José A. Gavira

oxyHbII crystals have been grown at pH 4, 5, 8 and 9 by capillary counterdiffusion technique by a two-step protocol: (i) mini screen (searching step) and (ii) pH screen (optimization step).

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 264–268. Published online 2010 February 24. doi: 10.1107/S1744309109053081
PMCID:
PMC2833032
Joana Rocha, Alma O. Popescu, Isabel Sá-Correia, Arsénio M. Fialho, Carlos Frazão

Crystals of B. cepacia IST408 UDP-glucose dehydrogenase (EC 1.1.1.22) were obtained in space group P212121 and diffracted to 2.09 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 269–271. Published online 2010 February 24. doi: 10.1107/S1744309109053500
PMCID:
PMC2833033
Huizheng Wang, Jiubiao Guo, Hai Pang, Xiuguo Zhang

The MaoC-like dehydratase from P. capsici was cloned, expressed and purified to homogeneity. Crystals were obtained that diffracted to 1.93 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 272–274. Published online 2010 February 24. doi: 10.1107/S1744309109053391
PMCID:
PMC2833034
Yasutoshi Kido, Tomoo Shiba, Daniel Ken Inaoka, Kimitoshi Sakamoto, Takeshi Nara, Takashi Aoki, Teruki Honma, Akiko Tanaka, Masayuki Inoue, Shigeru Matsuoka, Anthony Moore, Shigeharu Harada, Kiyoshi Kita

Cyanide-insensitive alternative oxidase from T. brucei brucei has been purified and crystallized for X-ray structure analysis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 275–278. Published online 2010 February 24. doi: 10.1107/S1744309109054062
PMCID:
PMC2833035
Shigetarou Mori, Keigo Shibayama, Jun-ichi Wachino, Yoshichika Arakawa

A novel diadenosine 5′,5′′′-P 1,P 4-tetraphosphate phosphorylase from M. tuberculosis H37Rv has been crystallized. The crystal belonged to space group C2 and diffracted X-rays to 1.9 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 279–281. Published online 2010 February 24. doi: 10.1107/S174430910905444X
PMCID:
PMC2833036
Miki Senda, Takashi Hatta, Kazuhide Kimbara, Toshiya Senda

A thermostable manganese(II)-dependent 2,3-dihydroxybiphenyl-1,2-dioxygenase derived from Bacillus sp. JF8 was crystallized in two forms using the sitting-drop vapour-diffusion method. Both crystals diffracted to approximately 1.3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 282–285. Published online 2010 February 24. doi: 10.1107/S1744309109054396
PMCID:
PMC2833037
Siqiang Niu, Miao Luo, Ailong Huang, Yibing Yin, Deqiang Wang

Here, the expression, purification, crystallization and preliminary crystallographic analysis of SP0987 from Streptococcus pneumoniae TIGR4 are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 286–288. Published online 2010 February 24. doi: 10.1107/S1744309110000163
PMCID:
PMC2833038
Wilson Wong, Ruth M. Kennan, Carlos J. Rosado, Julian I. Rood, James C. Whisstock, Corrine J. Porter

The subtilisin-like proteases AprV2 and BprV from virulent strains and AprB2 and BprB from benign strains of D. nodosus have been crystallized and X-ray diffraction data have been collected to 2.0, 2.0, 1.7 and 1.8 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 289–293. Published online 2010 February 24. doi: 10.1107/S1744309110000333
PMCID:
PMC2833039
Olga N. Pakhomova, Alexander B. Taylor, Argentina Becker, Stephen P. Holloway, T. R. Kannan, Joel B. Baseman, P. John Hart

Community-acquired respiratory distress syndrome toxin (CARDS TX) from M. pneumoniae was cloned, expressed, purified and crystallized. A diffraction data set from a native CARDS TX crystal was obtained at 2.2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 294–296. Published online 2010 February 24. doi: 10.1107/S1744309110000114
PMCID:
PMC2833040
Ángel Pereira-Rodríguez, Rafael Fernández-Leiro, M. Isabel González Siso, M. Esperanza Cerdán, Manuel Becerra, Julia Sanz-Aparicio

β-Galactosidase from K. lactis has been expressed in S. cerevisiae, purified by affinity chromatography and crystallized in its native form.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 297–300. Published online 2010 February 24. doi: 10.1107/S1744309109054931
PMCID:
PMC2833041
Hirofumi Komori, Daisuke Seo, Takeshi Sakurai, Yoshiki Higuchi

Ferredoxin-NADP+ oxidoreductase from B. subtilis has been crystallized by the sitting-drop vapour-diffusion method. Diffraction data were collected to 1.8 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 301–303. Published online 2010 February 24. doi: 10.1107/S1744309110000151
PMCID:
PMC2833042
Emmanuel Oluwadare Balogun, Daniel Ken Inaoka, Yasutoshi Kido, Tomoo Shiba, Takeshi Nara, Takashi Aoki, Teruki Honma, Akiko Tanaka, Masayuki Inoue, Shigeru Matsuoka, Paul A. M. Michels, Shigeharu Harada, Kiyoshi Kita

Glycerol kinase from human African trypanosomes has been purified and crystallized for X-ray structure analysis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 304–308. Published online 2010 February 24. doi: 10.1107/S1744309110000369
PMCID:
PMC2833043
Yasumitsu Sakamoto, Masakazu Ike, Nobutada Tanaka, Yoshiyuki Suzuki, Wataru Ogasawara, Hirofumi Okada, Takamasa Nonaka, Yasushi Morikawa, Kazuo T. Nakamura

An exo-β-d-glucosaminidase from T. reesei (Gls93) has been crystallized by the hanging-drop vapour-diffusion method. Diffraction data have been collected using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 309–312. Published online 2010 February 24. doi: 10.1107/S1744309110000606
PMCID:
PMC2833044
Masaaki Ishihara, Yoshio Kusakabe, Tsuyoshi Ohsumichi, Nobutada Tanaka, Masayuki Nakanishi, Yukio Kitade, Kazuo T. Nakamura

Mouse S-adenosyl-l-homocysteine hydrolase has been crystallized in the presence of the reaction product adenosine. Diffraction data to 1.55 Å resolution were collected using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 313–315. Published online 2010 February 24. doi: 10.1107/S1744309110000771
PMCID:
PMC2833045
Tiago M. Bandeiras, Célia V. Romão, João V. Rodrigues, Miguel Teixeira, Pedro M. Matias

Diffraction-quality crystals of A. fulgidus neelaredoxin have been produced. The expression, purification, crystallization and X-ray crystallographic analysis of A. fulgidus neelaredoxin are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 316–319. Published online 2010 February 24. doi: 10.1107/S1744309110000916
PMCID:
PMC2833046
Sompong Sansenya, James R. Ketudat Cairns, Rodjana Opassiri

Recombinant rice Os4BGlu12 β-glucosidase purified from E. coli was crystallized with and without 2,4-dinitrophenyl-2-deoxy-2-fluoro-β-d-glucopyranoside.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 320–323. Published online 2010 February 25. doi: 10.1107/S174430911000103X
PMCID:
PMC2833047
Asha Manikkoth Balakrishna, Cornelia Hunke, Gerhard Grüber

The full-length subunit E of the ATP synthase from P. horikoshii OT3 was cloned, overexpressed, purified and crystallized. The crystals belonged to space group I4 and diffracted to 3.3 Å resolution using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 324–326. Published online 2010 February 25. doi: 10.1107/S1744309110001016
PMCID:
PMC2833048
Mark A. Currie, Jarrett J. Adams, Sabrina Ali, Steven P. Smith, Zongchao Jia

A multimodular heterotrimeric complex encompassing a trimodular C-terminal fragment of the cellulosomal scaffoldin CipA from C. thermocellum bound to the type II cohesin module of SdbA and the type I dockerin module of CelD has been crystallized by the hanging-drop vapour-diffusion method and initial X-ray diffraction data analysis has been conducted.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 327–329. Published online 2010 February 25. doi: 10.1107/S1744309110001375
PMCID:
PMC2833049
Tomonobu Umeda, Nobutada Tanaka, Yoshio Kusakabe, Masayuki Nakanishi, Yukio Kitade, Kazuo T. Nakamura

1-Deoxy-d-xylulose 5-phosphate reductoisomerase from P. falciparum has been crystallized in the presence of NADPH. Diffraction data to 1.85 Å resolution have been collected using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 330–332. Published online 2010 February 25. doi: 10.1107/S1744309110001739
PMCID:
PMC2833050
Ken-ichi Aoki, Nobutada Tanaka, Yoshio Kusakabe, Chiharu Fukumi, Arayo Haga, Masayuki Nakanishi, Yukio Kitade, Kazuo T. Nakamura

Phosphoglucose isomerase from P. falciparum has been crystallized. Diffraction data to 1.8 Å resolution have been collected using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 333–336. Published online 2010 February 25. doi: 10.1107/S1744309110001740
PMCID:
PMC2833051
Ulrika W. Hultdin, Stina Lindberg, Christin Grundström, Anders Allgardsson, Shenghua Huang, Gunter Stier, Anders Öhman, Bernt Eric Uhlin, A. Elisabeth Sauer-Eriksson

The focB gene from E. coli was cloned and the protein was expressed and purified. The protein is oligomeric, most likely in the form of dimers. NMR analysis showed that line broadening significantly increases at temperatures below 293 K, presumably owing to increased conformational exchange or oligomerization. Crystals were obtained at room temperature and diffracted to better than 2.0 Å

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 337–341. Published online 2010 February 25. doi: 10.1107/S1744309110002204
PMCID:
PMC2833052
Junko Kato, Masato Kato

The catalytic subunits of the human pyruvate dehydrogenase phosphatase isoforms 1 and 2 have been purified and crystallized. The crystals of PDP1c and PDP2c diffracted to 2.45 and 2.0 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 342–345. Published online 2010 February 27. doi: 10.1107/S1744309110003131
PMCID:
PMC2833053
Annelies Van Hoorebeke, Jan Stout, John Kyndt, Manu De Groeve, Ina Dix, Tom Desmet, Wim Soetaert, Jozef Van Beeumen, Savvas N. Savvides

Preliminary crystallographic studies of recombinant cellobiose phosphorylase from Cellulomonas uda in complex with reaction substrates and products have led to high-quality diffraction data at high resolution, thus enabling structural studies to dissect the structural and mechanistic determinants of disaccharide phosphorylase activity.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 346–351. Published online 2010 February 27. doi: 10.1107/S1744309110002642
PMCID:
PMC2833054
Tewfik Soulimane, Sarah R. O’Kane, Olga Kolaj

Microcrystals of HpaB were formed while isolating integral membrane respiratory complexes from Thermus thermophilus. Further purification of the enzyme was achieved by repetitive crystallization. Subsequently, well shaped single crystals of the native enzyme that diffracted to 1.82 Å resolution were grown in sitting drops.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 352–356. Published online 2010 February 27. doi: 10.1107/S1744309110003714
PMCID:
PMC2833055
Ari Ora, Esko Oksanen, Tommi Kajander, Adrian Goldman, Sarah J. Butcher

Mouse peroxiredoxin II was crystallized in an orthorhombic space group and native X-ray diffraction data were collected.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 357–360. Published online 2010 February 27. doi: 10.1107/S1744309110003684
PMCID:
PMC2833056
Henning Tidow, Kim L. Hein, Lone Baekgaard, Michael G. Palmgren, Poul Nissen

Plant plasma-membrane Ca2+-ATPase is regulated via binding of calmodulin to its autoinhibitory N-terminal domain. In this study, the expression, purification, crystallization and preliminary X-ray diffraction analysis of this protein complex from A. thaliana are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 361–363. Published online 2010 February 27. doi: 10.1107/S1744309110003805
PMCID:
PMC2833057

Laboratory Communications

Harindarpal S. Gill

The effectiveness of using ultraviolet microscopes to illuminate protein crystals in high throughput screens is evaluated.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 March 1; 66(Pt 3): 364–372. Published online 2010 February 27. doi: 10.1107/S1744309110002022
PMCID:
PMC2833058

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography