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Volume 63(Pt 5);  2007 May 1

Protein Structure Communications

Obidimma C. Ezezika, Sandra Haddad, Ellen L. Neidle, Cory Momany

A general tetramerization and oligomerization scheme is proposed for BenM and other LysR-type transcriptional regulators based on two newly characterized structures of the effector-binding domain of BenM. An analysis of these structures in the light of other LysR-type structures may explain the general solubility problems associated with this protein family.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 361–368. Published online 2007 April 28. doi: 10.1107/S1744309107019185
PMCID:
PMC2334995

Structural Genomics Communications

Vaheh Oganesyan, Paul D. Adams, Jarmila Jancarik, Rosalind Kim, Sung-Hou Kim

Using single-wavelength anomalous dispersion data obtained from a gold-derivatized crystal, the X-ray crystal structure of the protein 067745_AQUAE from the prokaryotic organism Aquifex aeolicus has been determined to a resolution of 2.0 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 369–374. Published online 2007 April 28. doi: 10.1107/S1744309107018945
PMCID:
PMC2335016

Crystallization Communications

Ryo Takahashi, Shota Nakamura, Takuya Yoshida, Yuji Kobayashi, Tadayasu Ohkubo

Human nicotinamide phosphoribosyltransferase has been crystallized using microseeding methods and X-ray diffraction data have been collected at 2.0 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 375–377. Published online 2007 April 6. doi: 10.1107/S1744309107006069
PMCID:
PMC2335003
Ruhu Qi, Susanne Fetzner, Aaron J. Oakley

Preliminary crystallographic data for 1H-3-hydroxy-4-oxoquinoline 2,4-dioxygenase from P. putida 33/1 are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 378–381. Published online 2007 April 6. doi: 10.1107/S1744309107013760
PMCID:
PMC2335004
Roberto A. Steiner, Ursula Frerichs-Deeken, Susanne Fetzner

Preliminary crystallographic data are reported for 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) from A. nitroguajacolicus Rü61a.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 382–385. Published online 2007 April 6. doi: 10.1107/S174430910701353X
PMCID:
PMC2335005
Pojchong Chotiyarnwong, Guillaume B. Stewart-Jones, Michael J. Tarry, Wanwisa Dejnirattisai, Christian Siebold, Michael Koch, David I. Stuart, Karl Harlos, Prida Malasit, Gavin Screaton, Juthathip Mongkolsapaya, E. Yvonne Jones

Crystals of an MHC class I molecule bound to naturally occurring peptide variants from the dengue virus NS3 protein contained high levels of solvent and required optimization of cryoprotectant and dehydration protocols for each complex to yield well ordered diffraction, a process facilitated by the use of a free-mounting system.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 386–392. Published online 2007 April 6. doi: 10.1107/S1744309107013693
PMCID:
PMC2334998
Naoko Shimada, Bunzo Mikami, Seiya Watanabe, Keisuke Makino

l-2-Keto-3-deoxyarabonate dehydratase was overexpressed, purified and crystallized at 291 K using the hanging-drop vapour-diffusion method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 393–395. Published online 2007 April 6. doi: 10.1107/S1744309107015102
PMCID:
PMC2334997
Kenji Murata, Andrew J. Fisher, Jerry L. Hedrick

The 24 kDa egg lectin of Chinook salmon (Oncorhynchus tshawytscha) was purified by affinity chromatography from salmon eggs and crystallized by the hanging-drop vapor-diffusion method using 15/4 EO/OH (pentaerythritol ethoxylate) as a precipitant.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 396–398. Published online 2007 April 6. doi: 10.1107/S1744309107015345
PMCID:
PMC2335001
Michael A. Joyce, Edward R. Brownie, Koto Hayakawa, Marie E. Fraser

Attempts to crystallize succinyl-CoA synthetase from the thermophile T. aquaticus were thwarted by proteolysis of the β-subunit and preferential crystallization of a truncated form. Crystals of the full-length enzyme were grown after the purification protocol was modified to include frequent additions of protease inhibitors.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 399–402. Published online 2007 April 14. doi: 10.1107/S1744309107017113
PMCID:
PMC2335007
Takuto Matsui, Shinsuke Nishimura, Kenichi Kijima, Yasushi Kawasaki, Fumio Tashiro, Shigetoshi Miura

Crystals of the rat histone H10 globular domain have been grown in the trigonal space group P3121 and diffracted to a resolution of 1.98 Å. Molecular-replacement solutions were identified and refinement has been started.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 403–405. Published online 2007 April 14. doi: 10.1107/S1744309107015357
PMCID:
PMC2334994
Bin Ren, Joëlle Kuhn, Laurence Meslet-Cladiere, Hannu Myllykallio, Rudolf Ladenstein

A RecB-like nuclease from the archaeon Pyrococcus abyssi was expressed, purified and crystallized. The crystals belong to the orthorhombic space group C2221 with a = 81.5, b = 159.8, c = 100.8 Å, and a native data set was collected to 2.65 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 406–408. Published online 2007 April 14. doi: 10.1107/S1744309107015278
PMCID:
PMC2334999
Mamta Bajaj, Hideaki Moriyama

The first crystallization of deoxyuridine triphosphate nucleotidohydrolase from plant, Arabidopsis thaliana, has been performed. An additive, taurine, was effective in producing the single crystal.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 409–411. Published online 2007 April 14. doi: 10.1107/S1744309107016004
PMCID:
PMC2335013
Neratur K. Lokanath, Kudigana J. Pampa, Toshimi Kamiya, Naoki Kunishima

A putative UDP-N-acetyl-d-mannosamine dehydrogenase from P. horikoshii OT3 has been crystallized in space group P21, with unit-cell parameters a = 80.28, b = 69.24, c = 83.10 Å, β = 114.4°. X-ray diffraction data have been collected to 1.80 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 412–414. Published online 2007 April 14. doi: 10.1107/S1744309107016685
PMCID:
PMC2334993
Carrie-Lynn Keiski, Patrick Yip, Howard Robinson, Lori L. Burrows, P. Lynne Howell

The cloning, expression, purification and crystallization of wild-type AlgK from P. fluorescens is described. The crystals diffract to 2.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 415–418. Published online 2007 April 14. doi: 10.1107/S1744309107016880
PMCID:
PMC2335008
Nicolas Soler, Dominique Fourmy, Satoko Yoshizawa

The mRNA-binding domain of E. coli selenocysteine-specific elongation factor SelB (residues 478–614; SelB-WH3/4) was overproduced in E. coli and its cognate mRNA ligand, 23 nucleotides of the SECIS RNA hairpin, was prepared by in vitro transcription. The purified SelB-WH3/4–SECIS RNA complex crystallized in space group C2 and diffracted to 2.3 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 419–421. Published online 2007 April 20. doi: 10.1107/S174430910701723X
PMCID:
PMC2335015
Ying Peng, Feng Xu, Stephen G. Bell, Luet-Lok Wong, Zihe Rao

Palustrisredoxin reductase (RPA3782, PuR), a flavin-dependent ferredoxin reductase, is an essential component of the Class I cytochrome P450 systems in Rhodopseudomonas palustris CGA009. Crystals of PuR that diffract to 2.2 Å resolution have been obtained.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 422–425. Published online 2007 April 20. doi: 10.1107/S1744309107017411
PMCID:
PMC2335014
X. Lois Hermo-Parrado, Pablo Guardado-Calvo, Antonio L. Llamas-Saiz, Gavin C. Fox, Lorena Vazquez-Iglesias, José Martínez-Costas, Javier Benavente, Mark J. van Raaij

The avian reovirus double-stranded RNA-binding and core protein σA has been crystallized in space group P1, with unit-cell parameters a = 103.2, b = 129.9, c = 144.0 Å, α = 93.8, β = 105.1, γ = 98.2°. A complete data set has been collected to 2.3 Å resolution and analyzed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 426–429. Published online 2007 April 20. doi: 10.1107/S1744309107017988
PMCID:
PMC2335010
E. Bartholomeus Kuettner, Thomas M. Kriegel, Antje Keim, Manfred Naumann, Norbert Sträter

Crystals of the enzyme hexokinase 1 from the yeast K. lactis (KlHxk1) suitable for X-ray analysis were obtained in various space groups.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 430–433. Published online 2007 April 20. doi: 10.1107/S1744309107018271
PMCID:
PMC2335009
Julia M. Richardson, David J. Finnegan, Malcolm D. Walkinshaw

Full-length Mos1 transposase has been crystallized in a complex with inverted-repeat DNA. Crystals diffract to 3.25 Å resolution and display noncrystallographic twofold symmetry.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 434–437. Published online 2007 April 20. doi: 10.1107/S1744309107019045
PMCID:
PMC2335011
Jhe-Le Tu, Ko-Hsin Chin, Andrew H.-J. Wang, Shan-Ho Chou

A bacterial UMP kinase from the plant pathogen X. campestris pathovar campestris has been overexpressed in E. coli, purified and crystallized in a strong magnetic field. The crystals diffracted to 2.35 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 438–442. Published online 2007 April 20. doi: 10.1107/S1744309107018787
PMCID:
PMC2335002
Masaya Yamaguti, Nobuo N. Suzuki, Yuko Fujioka, Yoshinori Ohsumi, Fuyuhiko Inagaki

S. cerevisiae Atg10, an E2-like enzyme that mediates the conjugation reaction between Atg12 and Atg5, was crystallized and diffracted to 2.3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 443–445. Published online 2007 April 21. doi: 10.1107/S1744309107017472
PMCID:
PMC2335012
Shankar Prasad Kanaujia, Chellamuthu Vasuki Ranjani, Jeyaraman Jeyakanthan, Masami Nishida, Yoshiaki Kitamura, Seiki Baba, Akio Ebihara, Nobutaka Shimizu, Noriko Nakagawa, Akeo Shinkai, Masaki Yamamoto, Seiki Kuramitsu, Yoshitsugu Shiro, Kanagaraj Sekar, Shigeyuki Yokoyama

The glucose dehydrogenase (GDH) protein from T. thermophilus HB8 was cloned, expressed, purified and crystallized. GDH crystals belong to space group P21 and diffract to 1.9 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 446–448. Published online 2007 April 28. doi: 10.1107/S1744309107014819
PMCID:
PMC2334996
Gert Bange, Georg Petzold, Klemens Wild, Irmgard Sinning

Preliminary crystallographic data are reported for the third SRP GTPase FlhF from Bacillus subtilis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 449–451. Published online 2007 April 28. doi: 10.1107/S1744309107020180
PMCID:
PMC2335006
Jaldappagari Seetharamappa, Muse Oke, Huanting Liu, Stephen A. McMahon, Kenneth A. Johnson, Lester Carter, Mark Dorward, Michal Zawadzki, Ian M. Overton, C. A. Johannes van Niekirk, Shirley Graham, Catherine H. Botting, Garry L. Taylor, Malcolm F. White, Geoffrey J. Barton, Peter J. Coote, James H. Naismith

As part of work on S. aureus, the crystallization of Sar2028, a protein that is upregulated in MRSA, is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1; 63(Pt 5): 452–456. Published online 2007 April 28. doi: 10.1107/S1744309107019562
PMCID:
PMC2335000

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography