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Volume 64(Pt 2);  2008 February 1

Protein Structure Communications

Sara Lejon, Jacob Flyvholm Cramer, Peter Nordberg

The crystal structure of the complex formed by human serum albumin and a bacterial albumin-binding domain with the drug compound naproxen and fatty acid decanoate bound to albumin is described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 64–69. Published online 2008 January 18. doi: 10.1107/S174430910706770X
PMCID:
PMC2374170
Fabiana Lica Imai, Koji Nagata, Naoto Yonezawa, Minoru Nakano, Masaru Tanokura

The crystal structure of Ca2+-bound human S100A13 at pH 7.5 is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 70–76. Published online 2008 January 31. doi: 10.1107/S1744309107068236
PMCID:
PMC2374179

Crystallization Communications

Orly Alber, Ilit Noach, Raphael Lamed, Linda J. W. Shimon, Edward A. Bayer, Felix Frolow

The cloning, expression, purification, crystallization and preliminary X-ray characterization of a novel class of cohesin module (type III) from the R. flavefaciens ScaE anchoring scaffoldin are described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 77–80. Published online 2008 January 18. doi: 10.1107/S1744309107067437
PMCID:
PMC2374186
Giri Gowda, Someswar Rao Sagurthi, H. S. Savithri, M. R. N. Murthy

The cloning, expression, purification, crystallization and preliminary X-ray crystallographic studies of mannose 6-phosphate isomerase from S. typhimurium are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 81–84. Published online 2008 January 18. doi: 10.1107/S1744309107067930
PMCID:
PMC2374180
Wanius Garcia, Regiane F. Travensolo, Nathalia C. Rodrigues, João R. C. Muniz, Célia S. Caruso, Eliana G. M. Lemos, Ana Paula U. Araujo, Emanuel Carrilho

Glutathione S-transferase from X. fastidiosa (xfGST) has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.23 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 85–87. Published online 2008 January 18. doi: 10.1107/S174430910706825X
PMCID:
PMC2374177
Martina Tylichová, Pierre Briozzo, David Kopečný, Julien Ferrero, Solange Moréra, Nathalie Joly, Jacques Snégaroff, Marek Šebela

A 2.8 Å resolution data set was collected from a crystal of a recombinant plant aminoaldehyde dehydrogenase from P. sativum. This enzyme oxidizes ω-aminoaldehydes arising from polyamine degradation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 88–90. Published online 2008 January 18. doi: 10.1107/S1744309107068522
PMCID:
PMC2374172
Pazilat Bahti, Shunmei Chen, Yang Li, Neil Shaw, Xuejun Zhang, Min Zhang, Chongyun Cheng, Gaojie Song, Jie Yin, Hua Zhang, Dongsheng Che, Abdulla Abbas, Hao Xu, Bi-Cheng Wang, Zhi-Jie Liu

A non-Pfam protein, AF1514, from A. fulgidus has been crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 91–93. Published online 2008 January 18. doi: 10.1107/S1744309107068649
PMCID:
PMC2374175
M. Kurz, I. Iturbe-Ormaetxe, R. Jarrott, S. L. O’Neill, K. A. Byriel, J. L. Martin, B. Heras

The first crystallization of a W. pipientis protein, α-DsbA1, was achieved using hanging-drop and sitting-drop vapour diffusion.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 94–97. Published online 2008 January 31. doi: 10.1107/S1744309108000055
PMCID:
PMC2374166
K. E. Van Straaten, A. Hoffort, D. R. J. Palmer, D. A. R. Sanders

Selenomethionine-substituted IDH was crystallized using the microbatch method. The crystals diffracted to beyond 2.0 Å resolution using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 98–101. Published online 2008 January 31. doi: 10.1107/S1744309108000328
PMCID:
PMC2374185
Sarani Rangarajan, Jeyaraman Jeyakanthan, Palappetty Mridula, Keiko Sakamoto, Yoshiaki Kitamura, Yoshihiro Agari, Akeo Shinkai, Akio Ebihara, Seiki Kuramitsu, Shigeyuki Yokoyama, Kanagaraj Sekar

The ribosomal protein (L30e) from M. jannaschii was cloned from the gene MJ1044, expressed, purified and crystallized. The crystal belongs to the primitive tetragonal space group P43 and diffracted to 1.9 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 102–104. Published online 2008 January 31. doi: 10.1107/S1744309108000341
PMCID:
PMC2374168
Kyung Hye Seo, Supangat, Hye Lim Kim, Young Shik Park, Che Ok Jeon, Kon Ho Lee

The 6-pyruvoyltetrahydropterin synthase from E. coli has been crystallized in two crystal forms. Diffraction data were collected from hexagonal- and rectangular-shaped crystals to 3.0 and 2.3 Å, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 105–107. Published online 2008 January 31. doi: 10.1107/S1744309108000626
PMCID:
PMC2374169
Jiahui Tao, Yunkun Wu, David Ron, Bingdong Sha

To investigate the mechanism by which P58(IPK) functions to promote protein folding within the ER, a P58(IPK) TPR fragment without the C-terminal J-domain has been crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 108–110. Published online 2008 January 31. doi: 10.1107/S1744309108000833
PMCID:
PMC2374174
Céline Lafaye, Thomas Iwena, Jean-Luc Ferrer, J. Simon Kroll, Mickael Griat, Laurence Serre

The Neisseria meningitidis genome possesses three genes encoding active DsbAs. To throw light on the reason for this genetic multiplicity, the three enzymes have been purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 111–114. Published online 2008 January 31. doi: 10.1107/S1744309108000754
PMCID:
PMC2374167
Michel Thépaut, Corinne Vivès, Guillaume Pompidor, Richard Kahn, Franck Fieschi

Crystals of the carbohydrate-recognition domain of human langerin were obtained that diffracted synchrotron radiation to 1.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 115–118. Published online 2008 January 31. doi: 10.1107/S1744309108001000
PMCID:
PMC2374187
Nenad Cicmil, Lu Shi

B. subtilis queD was overexpressed, purified and crystallized. A diffraction data set was collected to a resolution of 3.6 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 119–122. Published online 2008 January 31. doi: 10.1107/S1744309108000924
PMCID:
PMC2374184
Maren Hoffmann, Reinhard Braaz, Dieter Jendrossek, Oliver Einsle

The extracellular rubber-degrading enzyme rubber oxygenase A (RoxA) from Xanthomonas sp. strain 35Y has been crystallized and diffraction data have been collected to high resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 123–125. Published online 2008 January 31. doi: 10.1107/S1744309108001206
PMCID:
PMC2374182
Laura J. Byrnes, Adriana Badarau, Sergei B. Vakulenko, Clyde A. Smith

APH(2′′)-Ic is an enzyme that is responsible for high-level gentamicin resistance in E. gallinarum isolates. Crystals of the wild-type enzyme and three mutants have been prepared and a complete X-ray diffraction data set was collected to 2.15 Å resolution from an F108L crystal.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 126–129. Published online 2008 January 31. doi: 10.1107/S1744309108001450
PMCID:
PMC2374173
Young Jun An, Bo-Eun Ahn, Jung-Hye Roe, Sun-Shin Cha

A nickel uptake regulator (Nur) from Streptomyces coelicolor has been crystallized. A 2.4 Å native data set and a 3.0 Å Ni-MAD data set were collected using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 130–132. Published online 2008 January 31. doi: 10.1107/S1744309108001760
PMCID:
PMC2374181
Chang Zhang, Yuna Sun, Wei Wang, Yan Zhang, Ming Ma, Zhiyong Lou

Human calbindin-D28k has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.4 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 133–136. Published online 2008 January 31. doi: 10.1107/S1744309108001905
PMCID:
PMC2374171
Alena Stsiapanava, Tana Koudelakova, Mikalai Lapkouski, Martina Pavlova, Jiri Damborsky, Ivana Kuta Smatanova

Three mutants of the haloalkane dehalogenase DhaA derived from R. rhodochrous NCIMB 13064 were crystallized and diffracted to ultrahigh resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 137–140. Published online 2008 January 31. doi: 10.1107/S1744309108002066
PMCID:
PMC2374176
Christian Linke, Tom T. Caradoc-Davies, Thomas Proft, Edward N. Baker

The S. pyogenes laminin-binding protein Lbp, which is essential for adhesion to human laminin, has been expressed, purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 141–143. Published online 2008 January 31. doi: 10.1107/S1744309108002273
PMCID:
PMC2374178
Stephanie Ravaud, Klemens Wild, Irmgard Sinning

The overproduction, purification, crystallization and preliminary crystallographic studies of the native and selenomethionine-labelled P1 domain are reported here as a first step towards the elucidation of the molecular mechanism of YidC as a membrane-protein insertase.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 February 1; 64(Pt 2): 144–148. Published online 2008 January 31. doi: 10.1107/S1744309108002364
PMCID:
PMC2374188

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography