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Volume 66(Pt 1);  January 1, 2010
Editorial
Howard Einspahr, Manfred S. Weiss
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 1. Published online Dec 25, 2009. doi: 10.1107/S1744309109055298
PMCID:
PMC2805522
Structural Communications
Chan-Ju Wang, Nicola Laurieri, Areej Abuhammad, Edward Lowe, Isaac Westwood, Ali Ryan, Edith Sim
Tyr131 plays an important role in determining the substrate specificity and thermal stability of azoreductase 1 from P. aeruginosa (pAzoR1). The structure shows that the substrate methyl red binds deeper in the active site of the mutant, which is reflected in the change in enzymic properties.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 2–7. Published online Dec 25, 2009. doi: 10.1107/S1744309109044741
PMCID:
PMC2805523
Damodharan Lakshminarasimhan, Subramaniam Eswaramoorthy, Stephen K. Burley, Subramanyam Swaminathan
The crystal structure of the hypothetical protein YqgQ from B. subtilis is reported at 2.1 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 8–11. Published online Dec 25, 2009. doi: 10.1107/S1744309109047009
PMCID:
PMC2805524
Crystallization Communications
Jens Radzimanowski, Stephanie Ravaud, Andrea Schott, Sabine Strahl, Irmgard Sinning
Overexpression, purification, crystallization and preliminary X-ray diffraction of the stromal-cell-derived factor 2-like protein of Arabidopsis thaliana are reported. The crystals belonged to the space group P61 and diffracted to 1.95 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 12–14. Published online Dec 25, 2009. doi: 10.1107/S1744309109042018
PMCID:
PMC2805525
Filipp Frank, Geneviève Virgili, Nahum Sonenberg, Bhushan Nagar
The MIF4G domain of DAP5 was crystallized in two distinct crystal forms. Diffraction patterns have been analyzed and preliminary analysis, including molecular replacement, is presented here.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 15–19. Published online Dec 25, 2009. doi: 10.1107/S1744309109044315
PMCID:
PMC2805526
Seung Kon Hong, Kook Han Kim, Eunice EunKyeong Kim
Malonyl-CoA:acyl-carrier protein transacylase (MCAT; FabD) from S. aureus has been cloned, overexpressed, purified and crystallized. The crystal belonged to space group P21, with unit-cell parameters a = 41.608, b = 86.717, c = 43.163 Å, α = γ = 90, β = 106.330°, and data were collected to 1.2 Å resolution using synchrotron radiation.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 20–22. Published online Dec 25, 2009. doi: 10.1107/S1744309109045989
PMCID:
PMC2805527
Koji Nishikawa, Yasuhito Shomura, Shinji Kawasaki, Youichi Niimura, Yoshiki Higuchi
NADH:rubredoxin oxidoreductase from C. acetobutylicum was expressed in E. coli, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.1 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 23–25. Published online Dec 25, 2009. doi: 10.1107/S1744309109047162
PMCID:
PMC2805528
Christopher D. Bahl, Daniel P. MacEachran, George A. O’Toole, Dean R. Madden
The P. aeruginosa virulence factor Cif, which inhibits cell-surface expression of the cystic fibrosis transmembrane conductance regulator, has been expressed, purified and crystallized.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 26–28. Published online Dec 25, 2009. doi: 10.1107/S1744309109047599
PMCID:
PMC2805529
Aleksandra A. Watson, Christopher A. O’Callaghan
Recombinant human CLEC5A was crystallized in the trigonal space group P31 and X-ray diffraction data were collected to 1.56 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 29–31. Published online Dec 25, 2009. doi: 10.1107/S1744309109047915
PMCID:
PMC2805530
Natalia E. Sibarani, Michael A. Gorman, Con Dogovski, Michael W. Parker, Matthew A. Perugini
The emergence of drug-resistant bacteria highlights the importance of identifying potential drug targets. Dihydrodipicolinate synthase (DHDPS) is a valid but as yet unexploited antimicrobial target that functions in the biosynthesis of (S)-lysine. In this study, the cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DHDPS from S. pneumoniae are described.
...
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 32–36. Published online Dec 25, 2009. doi: 10.1107/S174430910904771X
PMCID:
PMC2805531
Lilian Hor, Renwick C. J. Dobson, Con Dogovski, Craig A. Hutton, Matthew A. Perugini
Diaminopimelate (DAP) epimerase, an enzyme in the lysine-biosynthetic pathway, is a promising target for antibiotic development against pathogenic bacteria. Here, the cloning, expression, purification, crystallization and preliminary diffraction analysis of DAP epimerase from E. coli are reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 37–40. Published online Dec 25, 2009. doi: 10.1107/S1744309109047708
PMCID:
PMC2805532
Hwa Young Shin, Wonchull Kang, Sang Yoon Lee, Jin Kuk Yang
The N domain of p97/VCP was crystallized in complex with the UBX domain of FAF1. X-ray diffraction data were collected to 2.60 Å resolution and the crystals belonged to space group C2221.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 41–43. Published online Dec 25, 2009. doi: 10.1107/S1744309109047691
PMCID:
PMC2805533
Rafael Fernández-Leiro, Ángel Pereira-Rodríguez, M. Esperanza Cerdán, Manuel Becerra, Juliana Sanz-Aparicio
α-Galactosidase from S. cerevisiae has been purified and crystallized in glycosylated and deglycosylated states. X-ray diffraction data were collected to 1.95 Å resolution from the deglycosylated form.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 44–47. Published online Dec 25, 2009. doi: 10.1107/S1744309109047794
PMCID:
PMC2805534
Ha Yun Jung, Ki Jeung Lee, Kyung Ha Kim, Ji Hye Hyoung, Mi Ra Han, Hyun Kyoung Kim, Lin-Woo Kang, Yeh-Jin Ahn, Yong-Seok Heo
The GyrB subunit of DNA gyrase from X. oryzae pv. oryzae was expressed, purified and crystallized. Diffraction data were collected to a resolution of 2.10 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 48–50. Published online Dec 25, 2009. doi: 10.1107/S1744309109047721
PMCID:
PMC2805535
Giovanna Tosi, Simona Fermani, Giuseppe Falini, Letizia Polito, Massimo Bortolotti, Andrea Bolognesi
Stenodactylin is a type 2 RIP from the caudex of Adenia stenodactyla. Stenodactylin crystallization and preliminary X-ray diffraction data analysis are reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 51–53. Published online Dec 25, 2009. doi: 10.1107/S1744309109047654
PMCID:
PMC2805536
Young Jun An, Chang-Ro Lee, Supangat Supangat, Hyun Sook Lee, Jung-Hyun Lee, Sung Gyun Kang, Sun-Shin Cha
Lon is an oligomeric ATP-dependent protease that degrades defective or denatured proteins as well as some folded proteins for the control of cellular protein quality and metabolism. Lon from T. onnurineus NA1 has been purified and crystallized at 295 K.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 54–56. Published online Dec 25, 2009. doi: 10.1107/S1744309109048039
PMCID:
PMC2805537
Sudhir Dommaraju, Michael A. Gorman, Con Dogovski, F. Grant Pearce, Juliet A. Gerrard, Renwick C. J. Dobson, Michael W. Parker, Matthew A. Perugini
Given the recent rise in antimicrobial resistance, there is an urgent need to identify and characterize new antibiotic drug targets. One such target is dihydrodipicolinate reductase (DHDPR), which is an essential bacterial enzyme that catalyzes the second step in the lysine-biosynthesis pathway. In this paper, the cloning, expression, purification and crystallization of DHDPR from methicillin-resistant
...
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 57–60. Published online Dec 25, 2009. doi: 10.1107/S1744309109047964
PMCID:
PMC2805538
Eugenia Magracheva, Sergei Pletnev, Sergei Kotenko, Wei Li, Alexander Wlodawer, Alexander Zdanov
Interferon-λ1 and the extracellular domain of its receptor IFN-λ1R1 were expressed in insect cells and purified to homogenity. Ligand–receptor complexes of ligands expressed in insect cells and in E. coli were formed, purified by size-exclusion chromatography and crystallized. Preliminary X-ray studies showed that the crystals diffracted to better than 2.2 Å
...
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 61–63. Published online Dec 25, 2009. doi: 10.1107/S1744309109048817
PMCID:
PMC2805539
Shunsuke Tagami, Shun-ichi Sekine, Thirumananseri Kumarevel, Masaki Yamamoto, Shigeyuki Yokoyama
To elucidate which RNA polymerase structural state a particular T. thermophilus Gre-family protein (Gfh1) associates with, the T. thermophilus RNAP elongation complex was cocrystallized with Gfh1.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 64–68. Published online Dec 25, 2009. doi: 10.1107/S1744309109049215
PMCID:
PMC2805540
Joana Rocha, Ana Teresa Granja, Isabel Sá-Correia, Arsénio Fialho, Carlos Frazão
Crystals of S. elodea ATCC 31461 UDP-glucose dehydrogenase (EC 1.1.1.22) were obtained in space groups P622 and P43212 and diffracted to 2.4 and 3.4 Å resolution, respectively.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 69–72. Published online Dec 25, 2009. doi: 10.1107/S174430910904929X
PMCID:
PMC2805541
Xiaoyun Bai, Geng Meng, Guoming Li, Ming Luo, Xiaofeng Zheng
A C-terminal truncation construct of human PACSIN 1 (1–344) has been purified and crystallized. Diffraction data were collected to 3.0 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 73–75. Published online Dec 25, 2009. doi: 10.1107/S1744309109049549
PMCID:
PMC2805542
Anna Perederina, Olga Esakova, Chao Quan, Elena Khanova, Andrey S. Krasilnikov
This article describes the first successful crystallization of components of eukaryotic ribonucleases P/MRP. Yeast RNase MRP RNA domain P3 was crystallized in a complex with the proteins Pop6 and Pop7; the crystals diffracted to 3.25 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 76–80. Published online Dec 25, 2009. doi: 10.1107/S1744309109049707
PMCID:
PMC2805543
Marta Toth, Sergei Vakulenko, Clyde A. Smith
Aminoglycoside-2′′-phosphotransferase-IVa [APH(2′′)-IVa] is an enzyme that is responsible for high-level gentamicin resistance in E. casseliflavus isolates. Three different crystals of wild-type substrate-free APH(2′′)-IVa have been prepared and preliminary X-ray diffraction experiments have been undertaken on all three crystal forms.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 81–84. Published online Dec 25, 2009. doi: 10.1107/S1744309109050039
PMCID:
PMC2805544
Jonathan Elegheert, Debbie van den Hemel, Ina Dix, Jan Stout, Jozef Van Beeumen, Ann Brigé, Savvas N. Savvides
Of the four old yellow enzyme homologues found in S. oneidensis, SYE4 is the homologue most implicated in resistance to oxidative stress. SYE4 was recombinantly expressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 85–90. Published online Dec 25, 2009. doi: 10.1107/S1744309109050386
PMCID:
PMC2805545
César Santiago, Angel Gutiérrez-Rodríguez, Paul A. Tucker, Thilo Stehle, José M. Casasnovas
A complex of the measles virus hemagglutinin and the CD46 receptor representing the initial step of the cell infection has been crystallized.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 91–94. Published online Dec 25, 2009. doi: 10.1107/S1744309109050593
PMCID:
PMC2805546
Taichi Kumanomidou, Tomomi Nakagawa, Tsunehiro Mizushima, Atsuo Suzuki, Fuminori Tokunaga, Kazuhiro Iwai, Yukiko Yoshida, Keiji Tanaka, Takashi Yamane
The crystallization and preliminary X-ray diffraction studies of the Skp1–Fbg3 complex are reported. Crystallization by repeated microseeding using selected crystals as a source of microseeds is described.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 95–98. Published online Dec 25, 2009. doi: 10.1107/S1744309109050581
PMCID:
PMC2805547
Jianhua Zhang, Yong Chen, Feng Gao, Weihong Chen, Jianxun Qi, Chun Xia
Using a peptide derived from H5N1, a complex of duck MHC class I molecule (DuMHC I) with duck β2-­microglobulin (Duβ2m) was assembled and crystallized. Initial structure analysis indicated that the crystals did not contain the complete DuMHC I complex but instead contained DuMHC I α3-domain and Duβ2m subunits.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 99–101. Published online Dec 25, 2009. doi: 10.1107/S174430910905088X
PMCID:
PMC2805548
Jose Ignacio Baños-Sanz, Maider Villate, Julia Sanz-Aparicio, Charles Alistair Brearley, Beatriz González
Inositol 1,3,4,5,6-pentakisphosphate kinase from A. thaliana has been expressed in E. coli, purified and crystallized and diffraction data have been collected to 2.3 Å resolution. Two heavy-atom crystal derivatives are under study.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 102–106. Published online Dec 25, 2009. doi: 10.1107/S1744309109051057
PMCID:
PMC2805549
International Union of Crystallography
Notes for authors.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1, 2010; 66(Pt 1): 107–111. Published online Dec 25, 2009. doi: 10.1107/S1744309109047848
PMCID:
PMC2805550
Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of
International Union of Crystallography