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This journal provides early access to some articles.
Volume 65(Pt 3);  March 1, 2009
Structural Communications
Steven B. Larson, John S. Day, Chieugiang Nguyen, Robert Cudney, Alexander McPherson
The crystal structure of proteinase K cocrystallized with digalacturonic acid and HEPES was solved at 1.32 Å resolution. The disaccharide was rotationally disordered about a twofold axis, but nonetheless contributed to intermolecular lattice interactions.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 192–198. Published online Feb 12, 2009. doi: 10.1107/S1744309109002218
PMCID:
PMC2650472
Haruhiko Sakuraba, Kazunari Yoneda, Takenori Satomura, Ryushi Kawakami, Toshihisa Ohshima
The crystal structure of a hyperthermophilic d-tagatose 3-epimerase-related protein with a unique active-site architecture was determined.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 199–203. Published online Feb 14, 2009. doi: 10.1107/S1744309109002115
PMCID:
PMC2650453
Charles E. Nichols, Sarah Sainsbury, Jingshan Ren, Thomas S. Walter, Anil Verma, David K. Stammers, Nigel J. Saunders, Raymond J. Owens
The structure of the MarR-family regulator NMB1585 from N. meningitidis has been solved using data extending to 2.1 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 204–209. Published online Feb 26, 2009. doi: 10.1107/S174430910900414X
PMCID:
PMC2650471
Crystallization Communications
Louise Aigrain, Denis Pompon, Gilles Truan, Solange Moréra
A 2.5 Å resolution data set was collected from a crystal of a soluble chimeric form of NADPH-cytochrome P450 reductase (CPR) produced using a fusion gene composed of the yeast FMN and the human FAD domains. The chimeric protein was crystallized in a modified conformation compared with the previously solved structures.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 210–212. Published online Feb 12, 2009. doi: 10.1107/S1744309109000700
PMCID:
PMC2650470
Tales Rocha Moura, Gustavo Arruda Bezerra, Maria Julia Barbosa Bezerra, Cícero Silvano Teixera, Eduardo Henrique Salviano Bezerra, Raquel Guimarães Benevides, Bruno Anderson Matias da Rocha, Luiz Augusto Gomes de Souza, Plínio Delatorre, Celso Shiniti Nagano, Benildo Sousa Cavada
Canavalia boliviana lectin (Cbol) was purified using a Sephadex G-50 column and crystallized in the presence of X-Man by hanging-drop vapour diffusion at 293 K. After optimization, crystals suitable for diffraction were obtained using 0.1 M HEPES pH 7.5 and 3.0 M sodium formate.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 213–215. Published online Feb 12, 2009. doi: 10.1107/S1744309109000797
PMCID:
PMC2650465
Chin-Yuan Chang, Yin-Cheng Hsieh, Ting-Yi Wang, Chun-Jung Chen, Tung-Kung Wu
The aminoacylhistidine dipeptidase encoded by V. alginolyticus pepD has been overexpressed and crystallized.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 216–218. Published online Feb 12, 2009. doi: 10.1107/S174430910900092X
PMCID:
PMC2650446
Baisong Zheng, Wen Yang, Yuguo Wang, Yan Feng, Zhiyong Lou
The thermophilic cellulase FnCel5A from F. nodosum Rt17-B1 has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.4 Å resolution for the native enzyme and to 1.7 Å resolution for the selenomethionyl derivative.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 219–222. Published online Feb 12, 2009. doi: 10.1107/S1744309109001316
PMCID:
PMC2650467
Kjell O. Håkansson, Aida Ćurović
Recombinant nucleotide-binding domain of E. coli Mg-ATPase was expressed, purified and used to produce crystals that diffracted to 1.53 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 223–225. Published online Feb 12, 2009. doi: 10.1107/S1744309109001419
PMCID:
PMC2650444
Harkewal Singh, Richard L. Felts, Li Ma, Thomas J. Malinski, Michael J. Calcutt, Thomas J. Reilly, John J. Tanner
Two members of the class C family of bacterial nonspecific acid phosphatases have been cloned, expressed, purified and crystallized. One of the crystal forms exhibited epitaxial twinning.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 226–231. Published online Feb 12, 2009. doi: 10.1107/S1744309109001511
PMCID:
PMC2650447
Ricardo M. F. Leal, Susana C. M. Teixeira, Matthew P. Blakeley, Edward P. Mitchell, V. Trevor Forsyth
The LADI-III diffractometer at the Institut Laue–Langevin has been used to carry out the first neutron crystallographic study of a DNA oligonucleotide in the A conformation. The crystal size was 0.06 mm3, the smallest ever used successfully for a study of this type. The results provide evidence of unexpected base protonation and illustrate the opportunities that now exist for nucleic
...
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 232–235. Published online Feb 12, 2009. doi: 10.1107/S1744309109002668
PMCID:
PMC2650452
Takanori Muto, Daisuke Tsuchiya, Kosuke Morikawa, Hisato Jingami
The site-specific unglycosylated mutant of metabotropic glutamate receptor 3 extracellular domain has been overexpressed, purified, and crystallized. A complete data set has been collected to 2.35 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 236–241. Published online Feb 14, 2009. doi: 10.1107/S1744309109002267
PMCID:
PMC2650456
Yong-Fu Li, Steven Poole, Fatima Rasulova, Annette L. McVeigh, Stephen J. Savarino, Di Xia
Three fusion proteins were generated in order to resolve the atomic structure of the CFA/I fimbriae of enterotoxigenic E. coli. CfaEB is a fusion of the minor and major CFA/I subunits, while CfaBB and CfaBBB are tandem fusions of two and three repeats, respectively, of the major subunit. Each protein was crystallized and the crystal structures of each of these fusions were determined successively by
...
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 242–247. Published online Feb 14, 2009. doi: 10.1107/S1744309109001584
PMCID:
PMC2650451
Markus G. Rudolph, Julia G. Wittmann, Dagmar Klostermeier
Two C-terminal fragments of the RNA helicase Hera have been crytallized in three crystal forms, one of which was phased by MAD using a single selenium site.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 248–252. Published online Feb 14, 2009. doi: 10.1107/S1744309108043145
PMCID:
PMC2650459
Sarah C. Atkinson, Renwick C. J. Dobson, Janet M. Newman, Michael A. Gorman, Con Dogovski, Michael W. Parker, Matthew A. Perugini
Dihydrodipicolinate synthase (DHDPS) catalyzes an important step in lysine biosynthesis. Here, the crystallization and preliminary diffraction analysis to 1.2 Å resolution of DHDPS from C. botulinum in the presence of its substrate pyruvate is reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 253–255. Published online Feb 14, 2009. doi: 10.1107/S1744309108039018
PMCID:
PMC2650466
Hanna Koskiniemi, Thadee Grocholski, Gunter Schneider, Jarmo Niemi
The cofactor-independent monooxygenase SnoaB from the nogalamycin-biosynthesis pathway and a designed selenomethionine-substituted double mutant were crystallized in space group P21212. The native enzyme diffracted to a resolution of 2.4 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 256–259. Published online Feb 14, 2009. doi: 10.1107/S1744309109001389
PMCID:
PMC2650448
Ryan Latimer, Kateryna Podzelinska, Alexei Soares, Anupam Bhattacharya, Leo C. Vining, Zongchao Jia, David L. Zechel
CmlS from S. venezuelae is a flavin-dependent halogenase that is involved in the biosynthesis of the widely used antibiotic chloramphenicol. Here, the crystallization of CmlS and analysis of the initial diffraction data are reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 260–263. Published online Feb 26, 2009. doi: 10.1107/S1744309108043091
PMCID:
PMC2650468
Hirofumi Komori, Kentaro Miyazaki, Yoshiki Higuchi
A putative two-domain-type laccase from a metagenome was crystallized using the sitting-drop vapour-diffusion method. Diffraction data were collected to a resolution of 1.7 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 264–266. Published online Feb 26, 2009. doi: 10.1107/S1744309109002462
PMCID:
PMC2650455
Doyoun Kim, Hye-Yeon Hwang, Yang-Gyun Kim, Kyeong Kyu Kim
The Z-DNA-binding domain of a PKR-like kinase (PKZ) from goldfish was crystallized in complex with d(TCGCGCG)2. The crystals belonged to space group C2 and diffracted to 1.7 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 267–270. Published online Feb 26, 2009. doi: 10.1107/S1744309109002504
PMCID:
PMC2650443
Fengping Ye, Lianming Liang, Qili Mi, Jinkui Yang, Zhiyong Lou, Yuna Sun, Yu Guo, Zhaohui Meng, Keqin Zhang
Two cuticle-degrading proteases Ver112 and PL646 were purified from the nematophagous fungi L. psalliotae and P. lilacinus, respectively. The protease Ver112 and a complex between PL646 and a tetrapeptide inhibitor were crystallized. Diffraction data were collected to 1.65 and 2.2 Å resolution, respectively.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 271–274. Published online Feb 26, 2009. doi: 10.1107/S1744309109003595
PMCID:
PMC2650454
Milana Voronov-Goldman, Ilit Noach, Raphael Lamed, Linda J. W. Shimon, Ilya Borovok, Edward A. Bayer, Felix Frolow
A cohesin-like module from the hyperthermophilic archaeon A. fulgidus was cloned, expressed, purified and crystallized. X-ray diffraction data were collected to 1.82 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 275–278. Published online Feb 26, 2009. doi: 10.1107/S1744309109002887
PMCID:
PMC2650457
Shu Jie Li, Qing Zhao, Qiangjun Zhou, Yujia Zhai
Here, the C-terminal domain of the human voltage-gated proton channel Hv1 (C-Hv1) was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 279–281. Published online Feb 26, 2009. doi: 10.1107/S1744309109003777
PMCID:
PMC2650464
Xiao-Yan Liu, Jian Lei, Xiang Liu, Xiao-Dong Su, Lanfen Li
In order to further illustrate the catalytic mechanism of arginine decarboxylase by determining the three-dimensional structure of the enzyme the speA gene was amplified from B. subtilis genomic DNA and cloned. The enzyme was expressed in Escherichia coli and purified to homogeneity by nickel-chelation chromatography followed by size-exclusion chromatography. High-quality crystals were obtained
...
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 282–284. Published online Feb 26, 2009. doi: 10.1107/S1744309109003856
PMCID:
PMC2650450
Daisuke Iyaguchi, Min Yao, Isao Tanaka, Eiko Toyota
An RNA-binding region of human HuR bound to an 11-base RNA fragment has been crystallized. The crystals diffracted to a resolution of 1.8 Å and belonged to space group P212121.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 285–287. Published online Feb 26, 2009. doi: 10.1107/S174430910900400X
PMCID:
PMC2650458
Juan Wang, Yan-Feng Zhou, Lan-Fen Li, Xiao-Dong Su
α-Enolase from human liver (hENO1) was expressed as a soluble protein and purified by affinity column chromatography and gel filtration. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 2.5 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 288–290. Published online Feb 26, 2009. doi: 10.1107/S1744309109004138
PMCID:
PMC2650463
Gordon King, Justine M. Hill, Jennifer L. Martin, Joshua S. Mylne
A C-terminal fragment of VERNALIZATION1 from A. thaliana, a DNA-binding protein required for the acceleration of flowering in response to prolonged cold treatment, was crystallized. X-ray diffraction data were collected to a resolution of 2.1 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 291–294. Published online Feb 26, 2009. doi: 10.1107/S1744309109004217
PMCID:
PMC2650445
Yohei Mukai, Teruya Nakamura, Yasuo Yoshioka, Shin-ichi Tsunoda, Haruhiko Kamada, Shinsaku Nakagawa, Yuriko Yamagata, Yasuo Tsutsumi
The formation, crystallization and preliminary X-ray diffraction analysis of the tumour necrosis factor α (TNF)–tumour necrosis factor receptor type 2 (TNFR2) complex are described. The initial electron-density map, which was calculated using only the phases of refined TNF trimer structures, could detect the main chains and side chains of TNFR2 around the TNF trimer.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 295–298. Published online Feb 26, 2009. doi: 10.1107/S1744309109004461
PMCID:
PMC2650442
Kasper Runager, Raquel García-Castellanos, Zuzana Valnickova, Torsten Kristensen, Niels Chr. Nielsen, Gordon K. Klintworth, F. Xavier Gomis-Rüth, Jan J. Enghild
Wild-type and mutant recombinant human transforming growth factor β-induced protein (TGFBIp) were cloned, purified and crystallized. Preliminary X-ray crystallography data were obtained from wild-type TGFBIp.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 299–303. Published online Feb 26, 2009. doi: 10.1107/S1744309109005016
PMCID:
PMC2650473
Cristiane R. Guzzo, Chuck S. Farah
The cloning and expression of recombinant PilZXAC1133, a protein belonging to the PilZ superfamily, are described. PilZ proteins are associated with the control of several complex behaviours in bacteria and in some cases have been shown to bind c-diGMP. PilZXAC1133 containing selenomethionine produced crystals that diffracted to 1.85 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 304–306. Published online Feb 26, 2009. doi: 10.1107/S1744309109005545
PMCID:
PMC2650461
Pricila Hauk, Cristiane R. Guzzo, Paulo L. Ho, Chuck S. Farah
Recombinant selenomethionine-labelled LipL32, the major surface protein of pathogenic Leptospira, has been purified and crystallized. Data sets from two crystals were collected, one of which diffracted to 2.25 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 307–309. Published online Feb 26, 2009. doi: 10.1107/S1744309109005533
PMCID:
PMC2650462
SeungBum Kim, Sangbum Joo, Sangyoung Yoon, Sungsoo Kim, Jongkook Moon, Yeonwoo Ryu, Kyeong Kyu Kim, T. Doohun Kim
Est-Y29, a novel oligomeric class C β-lactamase from a metagenomic library, was crystallized in space group I41 and diffraction data were collected to 1.49 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 310–312. Published online Feb 26, 2009. doi: 10.1107/S1744309109005442
PMCID:
PMC2650449
M. Balasubramanian, Pon. Sathya Moorthy, K. Neelagandan, M. N. Ponnuswamy
Preliminary studies were carried out to purify and crystallize the sample from cat (Felis silvestris catus), a low oxygen-affinity haemoglobin in different crystal forms.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 313–316. Published online Feb 26, 2009. doi: 10.1107/S1744309109004503
PMCID:
PMC2650469
Walter R. P. Novak, Aaron G. Moulin, Matthew P. Blakeley, Ilme Schlichting, Gregory A. Petsko, Dagmar Ringe
A 2.0 Å resolution neutron diffraction data set has been collected from a D2O-soaked γ-chymotrypsin crystal at low pH on the Institute Laue–Langevin LADI-III beamline.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Mar 1, 2009; 65(Pt 3): 317–320. Published online Feb 26, 2009. doi: 10.1107/S1744309109006630
PMCID:
PMC2650460
Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of
International Union of Crystallography