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This journal provides early access to some articles.
Volume 65(Pt 7);  July 1, 2009
Structural Communications
Andrea R. Conlan, Mark L. Paddock, Herbert L. Axelrod, Aina E. Cohen, Edward C. Abresch, Sandra Wiley, Melinda Roy, Rachel Nechushtai, Patricia A. Jennings
The crystal structure of the anti-diabetic drug target mitoNEET obtained from a GFP fusion construct (1.4 Å resolution, R factor = 20.2%) shows that the CDGSH 2Fe–2S binding domains are superimposable with previously determined non-fused constructs. However, there is considerable flexibility in the position of the outer mitochondrial tethering arms resulting in two different
...
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 654–659. Published online Jun 27, 2009. doi: 10.1107/S1744309109019605
PMCID:
PMC2705628
Roland Pfoh, Jose A. Cuesta-Seijo, George M. Sheldrick
In the presence of Mn2+, a new crystal form of an echinomycin–d(ACGTACGT) complex is found which shows mixed base pairing next to the bis-intercalation site.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 660–664. Published online Jun 27, 2009. doi: 10.1107/S1744309109019654
PMCID:
PMC2705629
Eugenia Magracheva, Serguei Kozlov, Colin L. Stewart, Alexander Wlodawer, Alexander Zdanov
The crystal structure of the R482W mutant of the C-terminal domain of lamin A/C, which is responsible for familial partial lipodystrophy, has been determined at 1.5 Å resolution. A completely novel aggregation state of the mutated protein may be responsible for the changes in biological activity.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 665–670. Published online Jun 27, 2009. doi: 10.1107/S1744309109020302
PMCID:
PMC2705630
Vicky G. Tsirkone, Kyriaki Dossi, Christina Drakou, Spyros E. Zographos, Maria Kontou, Demetres D. Leonidas
The structure of ribonuclease A in complex with uridine 5′-phosphate and uridine 5′-diphosphate has been determined at 1.4 Å resolution in order to facilitate the rational design of selective and potent inhibitors.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 671–677. Published online Jun 27, 2009. doi: 10.1107/S1744309109021423
PMCID:
PMC2705631
Crystallization Communications
Yasunori Nakashima, Hiroshi Nii, Blythe E. Janowiak, Owen W. Griffith, Takao Hibi
The S. agalactiae bifunctional ligase for glutathione synthesis has been crystallized. Native data were collected to 2.8 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 678–680. Published online Jun 27, 2009. doi: 10.1107/S1744309109018636
PMCID:
PMC2705632
S. S. Sundaresan, P. Ramesh, K. Sivakumar, M. N. Ponnuswamy
Purification, crystallization and preliminary X-ray analysis of haemoglobin from ostrich (Struthio camelus) has been carried out under 293 K temperature conditions. The ostrich is a large flightless bird which contains inositol tetrakisphosphate in erythrocytes and its whole blood oxygen affinity is higher. Efforts have been made to explore the structure–function relationship of ostrich
...
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 681–683. Published online Jun 27, 2009. doi: 10.1107/S1744309109019009
PMCID:
PMC2705633
Stefan Köster, Werner Kühlbrandt, Özkan Yildiz
The N-terminal domain of FeoB from Methanococcus jannaschii was overproduced, purified to homogeneity and crystallized in the presence of GTP and magnesium.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 684–687. Published online Jun 27, 2009. doi: 10.1107/S1744309109019216
PMCID:
PMC2705634
Andreas Winklmeier, Michael Weyand, Christina Schreier, Hans Robert Kalbitzer, Werner Kremer
The tetramerization domain of human Kv1.3 was cloned, expressed, purified and crystallized. The crystals belonged to space group I4 and diffracted to 1.2 Å resolution using synchrotron radiation.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 688–691. Published online Jun 27, 2009. doi: 10.1107/S1744309109019514
PMCID:
PMC2705635
Yang Liu, Huihao Zhou, Juanjuan Zhu, Yongxiang Gao, Liwen Niu, Jing Liu, Maikun Teng
An antibody–antigen complex consisting of a single-chain variable fragment of the potential therapeutic antibody chA21 and an N-terminal fragment (residues 1–192) of the human ErbB2 extracellular domain was expressed, purified and crystallized. X-ray diffraction data were collected to 2.45 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 692–694. Published online Jun 27, 2009. doi: 10.1107/S1744309109020107
PMCID:
PMC2705636
Xenia Bogdanović, Rajesh Kumar Singh, Johanna Hentschke, Bjarnheidur K. Gudmundsdóttir, Winfried Hinrichs
Crystallization and preliminary X-ray diffraction studies of AsaP1_E294A and AsaP1_E294Q, two inactive mutants of the toxic zinc metallopeptidase AsaP1 from A. salmonicida subsp. achromogenes, are reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 695–697. Published online Jun 27, 2009. doi: 10.1107/S1744309109020132
PMCID:
PMC2705637
A. T. Gonçalves, D. Marçal, M. A. Carrondo, F. J. Enguita
The expression, purification and crystallization of a glycerol dehydrogenase from S. typhimurium is decribed. The crystals diffracted to 3.5 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 698–701. Published online Jun 27, 2009. doi: 10.1107/S1744309109020296
PMCID:
PMC2705638
Kannan Raghunathan, Frank S. Vago, Terry Ball, Nafissa Yakubova, David Grindem, William J. Wedemeyer, Dennis N. Arvidson
Recombinant V. cholerae EpsH has been expressed, purified and crystallized. The crystals diffracted to 1.71 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 702–704. Published online Jun 27, 2009. doi: 10.1107/S1744309109020454
PMCID:
PMC2705639
Hannes Uchtenhagen, Samer Sourial, Rosmarie Friemann, Mariethe Ehnlund, Anna-Lena Spetz, Robert A. Harris, Chaithanya Madhurantakam, Adnane Achour
Neutralizing Fab fragments of the HIV-2-binding murine antibody 7C8 were generated after purification from hybridoma cell-culture supernatant. Crystallization conditions were determined and diffraction data were collected to 2.7 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 705–708. Published online Jun 27, 2009. doi: 10.1107/S1744309109020685
PMCID:
PMC2705640
Yu-He Liang, Xiang-Yu Liu, Juan Wang, Lan-Fen Li
In order to study the structure and function of B. subtilis glycinamide ribonucleotide transformylase, the purN gene was amplified, cloned into an expression vector and expressed in soluble form in Escherichia coli. The protein was purified to homogeneity and crystals suitable for X-ray data collection were obtained.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 709–711. Published online Jun 27, 2009. doi: 10.1107/S1744309109020703
PMCID:
PMC2705641
Nina Forsgren, Richard J. Lamont, Karina Persson
The C-terminal domain of the S. gordonii surface protein SspB has been expressed, purified and crystallized. Diffraction data have been collected to 2.1 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 712–714. Published online Jun 27, 2009. doi: 10.1107/S1744309109021046
PMCID:
PMC2705642
Matthew Mitchell, Hyun-Joo Nam, Adam Carter, Angela McCall, Chelsea Rence, Antonette Bennett, Brittney Gurda, Robert McKenna, Mark Porter, Yoshihisa Sakai, Barry J. Byrne, Nicholas Muzyczka, George Aslanidi, Sergei Zolotukhin, Mavis Agbandje-McKenna
The purification, crystallization and preliminary X-ray crystallographic analyses of the AAV9 viral capsid are reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 715–718. Published online Jun 27, 2009. doi: 10.1107/S1744309109021460
PMCID:
PMC2705643
Haiyan Zhao, Annie Heroux, Reuben D. Sequeira, Liang Tang
The regulatory domain of response regulator YycF from an essential two-component signal transduction system has been crystallized and X-ray data have been collected at 1.95 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 719–722. Published online Jun 27, 2009. doi: 10.1107/S1744309109022696
PMCID:
PMC2705644
Filipe Freire, Maria João Romão, Anjos L. Macedo, Susana S. Aveiro, Brian J. Goodfellow, Ana Luísa Carvalho
This manuscript describes the overexpression, purification and crystallization of human SOUL protein (hSOUL). hSOUL is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placenta.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 723–726. Published online Jun 27, 2009. doi: 10.1107/S174430910902291X
PMCID:
PMC2705645
Ae Kyung Park, Seung Min Bong, Jin Ho Moon, Young Min Chi
The response regulator DesR from S. pneumoniae was cloned, expressed, purified and crystallized. A complete data set was collected to 1.7 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 727–729. Published online Jun 27, 2009. doi: 10.1107/S1744309109023082
PMCID:
PMC2705646
Shabir Najmudin, Cecília Bonifácio, Américo G. Duarte, Sofia R. Pualeta, Isabel Moura, José J. G. Moura, Maria J. Romão
Diifraction data have been collected for the apo form of the orange protein from D. gigas to 2.25 Å resolution in-house and to beyond 2.0 Å resolution at ESRF, Grenoble. The crystals belonged to a trigonal space group, with unit-cell parameters a = 43, b = 43, c = 106 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 730–732. Published online Jun 30, 2009. doi: 10.1107/S1744309109023392
PMCID:
PMC2705647
Ritcha Mehra-Chaudhary, Carolyn E. Neace, Lesa J. Beamer
The enzyme phosphoglucosamine mutase from B. anthracis participates in the peptidoglycan-biosynthetic pathway. The expression, purification and crystallization of this enzyme are described; diffraction data have been collected to 2.7 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 733–735. Published online Jun 30, 2009. doi: 10.1107/S1744309109023409
PMCID:
PMC2705648
Dipak N. Patil, Preeti, Anshul Chaudhry, Ashwani K. Sharma, ­Shailly Tomar, Pravindra Kumar
A 21 kDa Kunitz-type proteinase inhibitor was purified from tamarind (T. indica) seeds, crystallized and characterized by X-ray diffraction.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 736–738. Published online Jun 30, 2009. doi: 10.1107/S1744309109023495
PMCID:
PMC2705649
Masashi Akiyama, Soichi Takeda, Koichi Kokame, Junichi Takagi, Toshiyuki Miyata
A fragment of the ADAMTS13 ancillary domains (ADAMTS13-DTCS) has been expressed, purified and crystallized and the crystals have been characterized by X-ray diffraction.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 739–742. Published online Jun 30, 2009. doi: 10.1107/S1744309109023410
PMCID:
PMC2705650
Yongbin Xu, Bo-Young Yun, Se-Hoon Sim, Kangseok Lee, Nam-Chul Ha
This article describes how CusB from E. coli was overexpressed and the recombinant protein purified using Ni–NTA affinity, Q anion-exchange and gel-filtration chromatography, and how the purified CusB protein was crystallized using the vapour-diffusion method.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Jul 1, 2009; 65(Pt 7): 743–745. Published online Jun 30, 2009. doi: 10.1107/S1744309109019873
PMCID:
PMC2705651
Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of
International Union of Crystallography