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Volume 64(Pt 11);  2008 November 1

Protein Structure Communications

Shinji Kakuda, Kazuhisa Okada, Hiroshi Eguchi, Kazuya Takenouchi, Wataru Hakamata, Masaaki Kurihara, Midori Takimoto-Kamimura

The crystal structure of the ligand-binding domain of rat VDR in complex with a nonsecosteroidal vitamin D3 analogue YR301 has been determined at 2.0 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 970–973. Published online 2008 October 31. doi: 10.1107/S1744309108026754
PMCID:
PMC2581693
Hironori Suzuki, Masato Kawasaki, Takeshi Kakiuchi, Hideki Shibata, Soichi Wakatsuki, Masatoshi Maki

Ca2+-bound and Zn2+-bound forms of ALG-2 were crystallized and X-ray diffraction data were collected to 3.1 and 3.3 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 974–977. Published online 2008 October 31. doi: 10.1107/S1744309108030297
PMCID:
PMC2581688
Matthew W. Vetting, James C. Errey, John S. Blanchard

The structure of Rv0802c was determined in an unliganded form to 2.0 Å resolution utilizing single-wavelength anomalous dispersion from a samarium soak that resulted in a single bound Sm3+:citrate2 complex.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 978–985. Published online 2008 October 31. doi: 10.1107/S1744309108031679
PMCID:
PMC2581710
Rui Hao, Lei Chen, Jia-Wei Wu, Zhi-Xin Wang

The crystal structure of the MH2 domain of Mad, which closely matches those of other Smad MH2 domains, is reported at 3.2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 986–990. Published online 2008 October 31. doi: 10.1107/S1744309108033034
PMCID:
PMC2581694

Crystallization Communications

Janet Newman, Tam M. Pham, Thomas S. Peat

This paper describes how four general optimization techniques, growth-rate modulation, fine screening, seeding and additive screening, have been adapted for automation in a medium-throughput crystallization service facility.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 991–996. Published online 2008 October 31. doi: 10.1107/S1744309108029667
PMCID:
PMC2581684
Qiangmin Zhang, Feng Gao, Jianxun Qi, Hao Cheng, Yiwei Liu, George F. Gao

2-Keto-3-deoxy-6-phosphogluconate aldolase from pathogenic S. suis, an attractive drug target, was cloned, overexpressed, purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 997–999. Published online 2008 October 25. doi: 10.1107/S1744309108028340
PMCID:
PMC2581687
Inmaculada Gómez García, Caren L. Freel Meyers, Christopher T. Walsh, David M. Lawson

Crystals of NovN, an O-carbamoyltransferase from S. spheroides, were obtained in monoclinic and orthorhombic forms and native X-ray data were recorded to a maximum of 2.3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1000–1002. Published online 2008 October 25. doi: 10.1107/S1744309108030145
PMCID:
PMC2581686
Hiroyoshi Matsumura, Motoyasu Adachi, Shigeru Sugiyama, Shino Okada, Megumi Yamakami, Taro Tamada, Koushi Hidaka, Yoshio Hayashi, Tooru Kimura, Yoshiaki Kiso, Tomoya Kitatani, Sho Maki, Hiroshi Y. Yoshikawa, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Ryota Kuroki, Yusuke Mori

In order to determine the protonation states of the residues within the active site of an HIV-1 protease–inhibitor complex, a crystal of HIV-1 protease complexed with inhibitor (KNI-272) was grown to a size of 1.4 mm3 for neutron diffraction study. The crystal diffracted to 2.3 Å resolution with sufficient quality for further structure determination.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1003–1006. Published online 2008 October 25. doi: 10.1107/S1744309108029679
PMCID:
PMC2581681
Zui Fujimoto, Hitomi Ichinose, Satoshi Kaneko

Exo-α-1,5-l-arabinofuranosidase from S. avermitilis NBRC14893 was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 2.2 Å and belonged to space group P212121.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1007–1009. Published online 2008 October 25. doi: 10.1107/S1744309108029692
PMCID:
PMC2581701
Hideaki Ogata, Aruna Goenka Agrawal, Amrit Pal Kaur, Richard Goddard, Wolfgang Gärtner, Wolfgang Lubitz

The crystallization of adenylylsulfate reductase isolated from the sulfate-reducing bacterium D. vulgaris Miyazaki F is described. The crystals diffracted to 1.7 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1010–1012. Published online 2008 October 25. doi: 10.1107/S1744309108029588
PMCID:
PMC2581697
Cong Chen, Kyung Hye Seo, Hye Lim Kim, Ningning Zhuang, Young Shik Park, Kon Ho Lee

The dihydropteridine reductase from D. discoideum has been crystallized. Diffraction data were collected from a rectangular-shaped crystal to 2.16 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1013–1015. Published online 2008 October 25. doi: 10.1107/S1744309108028479
PMCID:
PMC2581689
Myoung-Ki Hong, Jin-Kwang Kim, Hyesoon Kim, Junho Jung, Yeh-Jin Ahn, Jeong-Gu Kim, Byoung-Moo Lee, Lin-Woo Kang

Xoo2316 from X. oryzae pv. oryzae, a predicted 6-phosphogluconolactonase, the second enzyme of the pentose phosphate pathway, has been cloned, purified and crystallized. A native data set was collected to 2.4 Å resolution and a MAD data set was collected to 2.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1016–1018. Published online 2008 October 28. doi: 10.1107/S1744309108025852
PMCID:
PMC2581699
K. El Omari, K. Scott, B. Dhaliwal, J. Ren, N. G. A. Abrescia, J. Budworth, M. Lockyer, K. L. Powell, A. R. Hawkins, D. K. Stammers

Crystals of the nucleocapsid protein of human respiratory syncytial virus have been obtained in two forms, the better of which diffracted to 3.6 Å resolution and contained ten subunits in the asymmetric unit.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1019–1023. Published online 2008 October 28. doi: 10.1107/S1744309108031059
PMCID:
PMC2581706
Keizo Yamamoto, Hideo Miyake, Masami Kusunoki, Shigeyoshi Osaki

The crystallization and preliminary X-ray analysis of isomaltase is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1024–1026. Published online 2008 October 28. doi: 10.1107/S174430910803114X
PMCID:
PMC2581680
Yuko Kurahashi, Mitsuaki Sugahara, Hideo Ago, Sae Aoyama, Naoko Takahashi, Koji Takio, Michiko Katsukawa, Shozo Yamamoto, Masashi Miyano

The Fab fragment of a monoclonal anti-prostaglandin E2 antibody was prepared and its complex with prostaglandin E2 was crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1027–1030. Published online 2008 October 28. doi: 10.1107/S174430910803131X
PMCID:
PMC2581702
Phuong-Thuy Ho Ngo, Jin-Kwang Kim, Hyesoon Kim, Junho Jung, Yeh-Jin Ahn, Jeong-Gu Kim, Byoung-Moo Lee, Lin-Woo Kang

Peptide deformylase (PDF) catalyzes the removal of the N-formyl group from the N-terminus of nascent polypeptides. This process is essential for the survival of bacteria. As it is an antibacterial drug target, PDF from X. oryzae pv. oryzae was cloned, purified and crystallized for preliminary X-ray studies.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1031–1033. Published online 2008 October 28. doi: 10.1107/S1744309108031631
PMCID:
PMC2581682
Abel Garcia-Pino, Minh-Hoa Dao-Thi, Ehud Gazit, Roy David Magnuson, Lode Wyns, Remy Loris

Crystals of bacteriophage P1 Doc were grown in the free state, in complex with a 22-amino-acid C-terminal peptide of Phd and in complex with full-length Phd.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1034–1038. Published online 2008 October 28. doi: 10.1107/S1744309108031722
PMCID:
PMC2581698
Shin-ichi Terawaki, Ken Kitano, Toshio Hakoshima

The membrane-targeting domains of the Rac-specific guanine nucleotide-exchange factors Tiam1 and Tiam2 were purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1039–1042. Published online 2008 October 31. doi: 10.1107/S1744309108031692
PMCID:
PMC2581685
César Carrasco-López, Cesar Godoy, Blanca de las Rivas, Gloria Fernández-Lorente, José M. Palomo, José M. Guisán, Roberto Fernández-Lafuente, Martín Martínez-Ripoll, Juan A. Hermoso

BTL2, a thermostable enantioselective biocatalyst of interest for industrial applications, has been crystallized using the sitting-drop vapour-diffusion method. Preliminary X-ray data to 2.2 Å resolution allowed the determination of its unit-cell parameters and space group and initiation of its structure determination.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1043–1045. Published online 2008 October 31. doi: 10.1107/S1744309108031928
PMCID:
PMC2581703
Yuko Fujioka, Nobuo N. Noda, Minako Matsushita, Yoshinori Ohsumi, Fuyuhiko Inagaki

The coiled-coil domain of Atg16 was expressed, purified and crystallized. The resultant crystal diffracted to 2.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1046–1048. Published online 2008 October 31. doi: 10.1107/S1744309108031898
PMCID:
PMC2581691
Ching-Chang Cho, Ching-Wei Luo, Chun-Hua Hsu

PAT, an acetyltransferase from the archaeon S. solfataricus that specifically acetylates the chromatin protein Alba, was expressed, purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1049–1051. Published online 2008 October 31. doi: 10.1107/S1744309108031965
PMCID:
PMC2581692
Stephen A. McMahon, Muse Oke, Huanting Liu, Kenneth A. Johnson, Lester Carter, Nadia Kadi, Malcolm F. White, Gregory L. Challis, James H. Naismith

AcsD, an NRPS-independent siderophore synthetase, has been crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1052–1055. Published online 2008 October 31. doi: 10.1107/S1744309108032132
PMCID:
PMC2581690
Laurent Cappadocia, Jurgen Sygusch, Normand Brisson

The Whirly domain of StWhy2 was crystallized both in the free form and in complex with single-stranded DNA.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1056–1059. Published online 2008 October 31. doi: 10.1107/S1744309108032399
PMCID:
PMC2581700
Chunhui Jiang, Rui Bao, Yuxing Chen

Arrowhead protease inhibitor A, a member of the serine protease inhibitor family, from S. sagittifolia has been cloned, expressed, purified and crystallized in complex with bovine trypsin.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1060–1062. Published online 2008 October 31. doi: 10.1107/S1744309108032454
PMCID:
PMC2581705
Xiaotian Zhong, Madhavan Buddha, Guido Guidotti, Ron Kriz, Will Somers, Lidia Mosyak

The ecto-enzymatic domain of rat E-NTPDase1 CD39 was expressed and purified and diffraction-quality crystals of the enzyme were obtained.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1063–1065. Published online 2008 October 31. doi: 10.1107/S1744309108032569
PMCID:
PMC2581708
Ankur Jain, Jörg Ziegler, David K. Liscombe, Peter J. Facchini, Paul A. Tucker, Santosh Panjikar

The plant enzyme pavine N-methyltransferase from T. flavum has been produced in E. coli, purified and crystallized and its structure has been solved.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1066–1069. Published online 2008 October 31. doi: 10.1107/S1744309108033046
PMCID:
PMC2581683
Wenzheng Zhang, Mingzhuo Zhao, Jianhui Li, Xuemei Li, ZongHao Zeng, Zihe Rao

The human Gadd45γ protein has been crystallized as a prelude towards determination of its three-dimensional structure by X-ray crystallography.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1070–1073. Published online 2008 October 31. doi: 10.1107/S174430910803306X
PMCID:
PMC2581695
Qing Xie, Heather M. Ongley, Joan Hare, Michael S. Chapman

Adeno-associated virus type 6, a human DNA virus that is being developed as a vector for gene therapy, has been crystallized in a form suitable for structure determination at about 3.2 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1074–1078. Published online 2008 October 31. doi: 10.1107/S1744309108033290
PMCID:
PMC2581704
Caroli Genis, Peyton Scone, Hideko Kasahara, Hyun-Joo Nam

The NKX2.5 homeodomain has been crystallized in complex with DNA. Diffraction data were collected to 1.7 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1079–1082. Published online 2008 October 31. doi: 10.1107/S1744309108033447
PMCID:
PMC2581709
Ana P. G. Silva, Robert T. Byrne, Maria Chechik, Callum Smits, David G. Waterman, Alfred A. Antson

MTH909, the M. thermautotrophicus orthologue of S. cerevisiae TAN1, has been overexpressed, purified and crystallized. X-ray data were collected to 2.85 Å resolution from a crystal belonging to space group P6122 (or P6522).

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1083–1086. Published online 2008 October 31. doi: 10.1107/S1744309108034039
PMCID:
PMC2581707
Hitoshi Yamamoto, Naoki Kunishima

The putative enolase MJ0232 from M. jannaschii was purified and crystallized. A native data set was collected to 1.85 Å resolution. Preliminary crystallographic analysis and a dynamic light-scattering experiment suggested biological relevance of the octameric state of MJ0232 in solution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 November 1; 64(Pt 11): 1087–1090. Published online 2008 October 31. doi: 10.1107/S1744309108034180
PMCID:
PMC2581696

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography