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This journal provides early access to some articles.
Volume 64(Pt 10);  October 1, 2008
Protein Structure Communications
James E. Spoonamore, Sue A. Roberts, Annie Heroux, Vahe Bandarian
The X-ray crystal structure of a 6-pyruvoyltetrahydropterin synthase homolog of unknown function has been determined at 1.5 Å resolution. The protein retains residues required for pterin binding, but nearly all catalytic residues are missing.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 875–879. Published online Sep 30, 2008. doi: 10.1107/S1744309108027048
PMCID:
PMC2564891
David Veesler, Stéphanie Blangy, Christian Cambillau, Giuliano Sciara
Crystals of an E. coli AcrB contaminant were grown from 95% pure CorA preparations. This very frequently occurring problem in membrane-protein crystallography laboratories is reported, as well as suggestions to avoid it.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 880–885. Published online Sep 30, 2008. doi: 10.1107/S1744309108028248
PMCID:
PMC2564894
Natasha N. Smith, D. Travis Gallagher
The structure and thermal melting data for dehydroquinase from A. fulgidus are reported. The protein melts in vitro well below the organism’s growth temperature.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 886–892. Published online Sep 30, 2008. doi: 10.1107/S1744309108028546
PMCID:
PMC2564895
Shanyun Lu, Craig D. Smith, Zhengrong Yang, Pamela S. Pruett, Lisa Nagy, Deborah McCombs, Lawrence J. DeLucas, Wayne J. Brouillette, Christie G. Brouillette
The crystal structure of apo nicotinic acid mononucleotide adenylyltransferase (NaMNAT) from B. anthracis was determined to 2.3 Å resolution and compared with other bacterial NaMNAT structures.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 893–898. Published online Sep 30, 2008. doi: 10.1107/S1744309108029102
PMCID:
PMC2564882
Crystallization Communications
Karin Felderer, Matthew Groves, Joachim Diez, Ehmke Pohl, Susanne Witt
Host–guest complexes of 20S proteasomes with either cytochrome c (cyt c) or green fluorescent protein (GFP) trapped inside the inner cavities of the proteasome have been crystallized. Diffraction data were collected to 3.4 Å (cyt c) and 3.8 Å (GFP) resolution using a synchrotron-radiation source.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 899–902. Published online Sep 30, 2008. doi: 10.1107/S1744309108026791
PMCID:
PMC2564888
Satoru Shimizu, Ella Czarina Magat Juan, Yu-ichiro Miyashita, Yoshiteru Sato, Md Mominul Hoque, Kaoru Suzuki, Masataka Yogiashi, Masaru Tsunoda, Anne-Catherine Dock-Bregeon, Dino Moras, Takeshi Sekiguchi, Akio Takénaka
Two types of putative threonyl-tRNA synthetases with complementary functions from the crenarchaea A. pernix K1 and S. tokodaii strain 7 have been overexpressed, purified and crystallized. The crystal structure of one of the four proteins, the catalytic type ThrRS from A. pernix, has successfully been solved by the Se-MAD method.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 903–910. Published online Sep 30, 2008. doi: 10.1107/S1744309108026924
PMCID:
PMC2564875
Shin-ichi Terawaki, Ken Kitano, Miki Aoyama, Toshio Hakoshima
The radixin FERM domain was shown to bind the MT1-MMP cytoplasmic peptide and crystals of the complex were obtained.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 911–913. Published online Sep 30, 2008. doi: 10.1107/S1744309108026869
PMCID:
PMC2564885
Adelson Joel da Silva, Rozeni Chagas Lima Teles, Gisele Ferreira Esteves, Camila Ramos dos Santos, João Alexandre Ribeiro Gonçalves Barbosa, Sonia Maria de Freitas
S. parahyba chymotrypsin inhibitor in complex with chymotrypsin has been purified and crystallized using the vapour-diffusion method and preliminarily analysis has been performed using X-ray diffraction.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 914–917. Published online Sep 30, 2008. doi: 10.1107/S1744309108026870
PMCID:
PMC2564883
Dessislava Georgieva, Anna Kardas, Friedrich Buck, Markus Perbandt, Christian Betzel
A novel l-amino-acid oxidase was isolated from V. ammodytes ammodytes venom and crystallized. The solution conditions under which the protein sample was monodisperse were optimized using dynamic light scattering prior to crystallization. Preliminary diffraction data were collected to 2.6 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 918–921. Published online Sep 30, 2008. doi: 10.1107/S1744309108027036
PMCID:
PMC2564880
Bénédicte Delagoutte, Nada Lallous, Catherine Birck, Pierre Oudet, Jean-Pierre Samama
The SET- and RING finger-associated (SRA) domain of human UHRF1 protein has been overexpressed in E. coli, purified and crystallized. The three-dimensional structure of the protein in its SeMet form was solved from 2.2 Å diffraction data using the multiple anomalous dispersion method.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 922–925. Published online Sep 30, 2008. doi: 10.1107/S1744309108027462
PMCID:
PMC2564892
Nguyen To Uyen, Kosuke Nishi, Suk-Youl Park, Ji-Woo Choi, Hyun-Ju Lee, Jeong-Sun Kim
The crystallization of the HsdR subunit of a putative type I restriction-modification system from V. vulnificus and the collection of diffraction data to 2.6 Å resolution are reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 926–928. Published online Sep 30, 2008. doi: 10.1107/S1744309108027516
PMCID:
PMC2564879
Somnath Mukherjee, Debajyoti Dutta, Baisakhee Saha, Amit Kumar Das
The cloning, overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) from MRSA252 are reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 929–932. Published online Sep 30, 2008. doi: 10.1107/S1744309108027504
PMCID:
PMC2564893
Cyril Dreyfus, David Pignol, Pascal Arnoux
A putative nicotianamine synthase from M. thermoautotrophicus was cloned, expressed, purified and crystallized. The native crystals diffracted to a resolution of 1.7 Å.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 933–935. Published online Sep 30, 2008. doi: 10.1107/S1744309108027796
PMCID:
PMC2564876
María Lucas, Danel Kortazar, Egoitz Astigarraga, José A. Fernández, Jose M. Mato, María Luz Martínez-Chantar, Luis Alfonso Martínez-Cruz
The C-terminal domain of the Methanococcus jannaschii protein MJ0100 includes a CBS-domain pair and has been overexpressed, purified and crystallized. Crystals of selenomethionine-substituted (SeMet) protein were also grown.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 936–941. Published online Sep 30, 2008. doi: 10.1107/S1744309108027930
PMCID:
PMC2564890
Shigeru Sugiyama, Yusuke Nomura, Taiichi Sakamoto, Tomoya Kitatani, Asako Kobayashi, Shin Miyakawa, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Yoshikazu Nakamura, Hiroyoshi Matsumura
An RNA aptamer in complex with the human IgG Fc fragment have been crystallized. The stirring technique with a rotary shaker was used to improve the crystals and to ensure that they were of high quality and single, resulting in crystals that diffracted to 2.2 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 942–944. Published online Sep 30, 2008. doi: 10.1107/S1744309108028236
PMCID:
PMC2564881
Kosei Takeda, Hiromi Yoshida, Goro Takada, Ken Izumori, Shigehiro Kamitori
Recombinant B. pallidus d-arabinose isomerase was crystallized and diffraction data were collected to 2.3 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 945–948. Published online Sep 30, 2008. doi: 10.1107/S1744309108028352
PMCID:
PMC2564884
Jonathan P. Schuermann, Tommi A. White, Dhiraj Srivastava, Dale B. Karr, John J. Tanner
The first diffraction-quality crystals of a PutA protein are reported. One of the three crystal forms described here exhibits pseudo-merohedral twinning. Removal of the N-terminal histidine tag aided the crystallization of another form.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 949–953. Published online Sep 30, 2008. doi: 10.1107/S174430910802842X
PMCID:
PMC2564878
Asami Hishiki, Toshiyuki Shimizu, Tomo Hanafusa, Haruo Ohmori, Mamoru Sato, Hiroshi Hashimoto
Crystallization and diffraction studies of a human PCNA–Polι peptide complex are reported.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 954–956. Published online Sep 30, 2008. doi: 10.1107/S1744309108028522
PMCID:
PMC2564886
Yueju Zhao, Yunhua Zhang, Feng Gao, Yanfen Xue, Yan Zeng, Yanhe Ma
The catalytic domain of an alkaline mannanase from the alkaliphilic Bacillus sp. N16-5 was expressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic analysis were performed for the recombinant enzyme.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 957–959. Published online Sep 30, 2008. doi: 10.1107/S1744309108028571
PMCID:
PMC2564887
Miki Senda, Shinsuke Muto, Masami Horikoshi, Toshiya Senda
The combination of leucine-to-methionine substitutions and optimization of cryoconditions improved the resolution of histone chaperone SET/TAF-Iβ/INHAT crystals from around 5.5 to 2.3 Å without changing the crystallization conditions, allowing successful structure determination of SET/TAF-Iβ/INHAT by the multiwavelength anomalous diffraction method.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 960–965. Published online Sep 30, 2008. doi: 10.1107/S1744309108028704
PMCID:
PMC2564889
Aisha Baba-Dikwa, Darren Thompson, Nick J. Spencer, Simon C. Andrews, Kimberly A. Watson
The YncE protein of E. coli has been overproduced and purified and preliminary crystallographic analysis has been performed to 2.1 Å resolution. The structure reveals a seven-bladed β-propeller fold.
Acta Crystallogr Sect F Struct Biol Cryst Commun. Oct 1, 2008; 64(Pt 10): 966–969. Published online Sep 30, 2008. doi: 10.1107/S1744309108029515
PMCID:
PMC2564877
Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of
International Union of Crystallography