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Volume 64(Pt 9);  2008 September 1

Protein Structure Communications

Guogang Xu, Xuejun Li, Peter W. Andrew, Garry L. Taylor

The structure of a catalytically active subdomain of the NanA sialidase from S. pneumoniae is reported to a resolution of 2.5 Å. The complex with the inhibitor Neu5Ac2en identifies the key catalytic residues and provides a platform for structure-based development of specific inhibitors.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 772–775. Published online 2008 August 20. doi: 10.1107/S1744309108024044
PMCID:
PMC2531273
Jacqueline Vitali, Michael J. Colaneri

The structure of the catalytic subunit of M. jannaschii aspartate transcarbamoylase has been determined in space group P212121 using synchrotron data to a resolution of 3.0 Å and was refined to a final R work and R free of 0.215 and 0.269, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 776–780. Published online 2008 August 20. doi: 10.1107/S1744309108025359
PMCID:
PMC2531265

Crystallization Communications

József Dobó, Veronika Harmat, Edina Sebestyén, László Beinrohr, Péter Závodszky, Péter Gál

Acidic nondenaturing PAGE revealed inhomogeneity of the MASP-1 catalytic region caused by deamidation as the reason behind previous unsuccessful crystallization attempts. Monitoring the separation of the various species by acidic nondenaturing PAGE during purification helped to obtain pure protein, which was subsequently crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 781–784. Published online 2008 August 9. doi: 10.1107/S174430910802294X
PMCID:
PMC2531277
Timothy M. Reed, Hidehiko Hirakawa, Minae Mure, Emily E. Scott, Julian Limburg

Histamine dehydrogenase from Nocardioides simplex has been expressed, purified and crystallized with full incorporation of 6-S-cysteinyl-FMN. Diffraction data have been collected to 2.7 Å resolution; the crystals belonged to the orthorhombic space group P212121.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 785–787. Published online 2008 August 9. doi: 10.1107/S1744309108023336
PMCID:
PMC2531280
Megen A. Miller, Emily E. Scott, Julian Limburg

Prolyl-4-hydroxylase from B. anthracis has been cloned, expressed and crystallized. A complete MAD data set has been collected to 1.4 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 788–791. Published online 2008 August 9. doi: 10.1107/S1744309108023439
PMCID:
PMC2531266
Vicki A. Bamford, Maria Armour, Sue A. Mitchell, Michaël Cartron, Simon C. Andrews, Kimberly A. Watson

Crystallization of the proposed FAD-containing ferri-siderophore reductase protein YqjH from E. coli has been performed and the structure has been determined to 3.0 Å resolution. The structure shows similarity to another proposed siderophore-interacting protein from S. putrefaciens and weak similarity to members of the NAD(P)H:flavin oxidoreductase superfamily.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 792–796. Published online 2008 August 9. doi: 10.1107/S174430910802352X
PMCID:
PMC2531270
Sarah Sainsbury, Jingshan Ren, Nigel J. Saunders, David I. Stuart, Raymond J. Owens

The full length and the regulatory domain of the LysR-type transcriptional regulator CrgA have been crystallized. Diffraction data were collected from two crystal forms of full-length CrgA to 3.0 and 3.8 Å resolution, respectively. Crystals of the selenomethionine derivative of the C-terminal regulatory domain of CrgA diffracted to 2.3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 797–801. Published online 2008 August 9. doi: 10.1107/S1744309108024068
PMCID:
PMC2531262
Lei Wei, Cheng Chen, Qi Zhao, Chun Li, Le Cong, Xiaoling Xu, Yanlin Ma, Ming Liao, Yuanyuan Xu, Zihe Rao

The crystal of the nsp3 ADRP domain of avian infectious bronchitis virus (IBV) has been obtained and subjected to further crystallograghic studies.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 802–804. Published online 2008 August 9. doi: 10.1107/S1744309108024391
PMCID:
PMC2531276
Jiang Yin, Craig R. Garen, Maia M. Cherney, Leonid T. Cherney, Michael N. G. James

N-Acetylglucosamine 1-phosphate uridyltransferase (GlmU) from M. tuberculosis H37Rv has been crystallized and preliminary X-ray crystallographic analysis has been performed. GlmU is a bi-domained bifunctional enzyme that is involved in the biosynthesis of UDP-N-acetylglucosamine, a precursor in peptidoglycan biosynthesis in M. tuberculosis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 805–808. Published online 2008 August 9. doi: 10.1107/S1744309108024500
PMCID:
PMC2531282
Alexandre Wohlkönig, Hélène Hodak, Bernard Clantin, Magalie Sénéchal, Coralie Bompard, Françoise Jacob-Dubuisson, Vincent Villeret

Par27 from B. pertussis, the prototype of a new group of parvulins has been crystallized in two different crystal forms.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 809–812. Published online 2008 August 9. doi: 10.1107/S1744309108024731
PMCID:
PMC2531267
Yuna Sun, Ming Li, Yan Zhang, Lifang Liu, Ye Liu, Zheng Liu, Xumei Li, Zhiyong Lou

G. zeae extracellular lipase has been overexpressed, purified and crystallized. Diffraction data were collected to 2.8 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 813–815. Published online 2008 August 20. doi: 10.1107/S1744309108019283
PMCID:
PMC2531274
Xiaoyan Zhao, Shenglan Wang, Hai Pang, Keqian Yang, Mark Bartlam

The calcium-binding protein encoded by the CalD gene in S. coelicolor A3(2) has been crystallized as a prelude towards the determination of its three-dimensional structure by X-ray crystallography.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 816–818. Published online 2008 August 20. doi: 10.1107/S1744309108019891
PMCID:
PMC2531264
Asami Hishiki, Toshiyuki Shimizu, Aya Serizawa, Haruo Ohmori, Mamoru Sato, Hiroshi Hashimoto

Crystallization and diffraction studies of human PCNA G178S mutant are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 819–821. Published online 2008 August 20. doi: 10.1107/S174430910802277X
PMCID:
PMC2531271
Andrey G. Baranovskiy, Nigar D. Babayeva, Youri I. Pavlov, Tahir H. Tahirov

The cloning, expression, purification and crystallization of the complex of the second and third regulatory subunits of human Pol δ are reported. The crystals were characterized and an X-ray diffraction data set was collected to a resolution of 3 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 822–824. Published online 2008 August 20. doi: 10.1107/S1744309108025086
PMCID:
PMC2531283
Byung-Cheon Jeong, Kyoung Shin Yoo, Kwang Wook Jung, Jeong Sheop Shin, Hyun Kyu Song

Native and selenomethionine-derivatized CDCP2 from A. thaliana have been overexpressed, purified and crystallized. The crystals diffracted to 2.4 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 825–827. Published online 2008 August 20. doi: 10.1107/S1744309108025128
PMCID:
PMC2531278
A. Bowyer, H. Mikolajek, J. N. Wright, A. Coker, P. T. Erskine, J. B. Cooper, Q. Bashir, N. Rashid, F. Jamil, M. Akhtar

The l-threonine dehydrogenase from T. kodakaraensis KOD1 has been crystallized in the tetragonal space group P43212, with unit-cell parameters a = b = 124.5, c = 271.1 Å. Diffraction data were collected to 2.6 Å resolution and preliminary analysis indicates that there are four molecules in the crystallographic asymmetric unit.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 828–830. Published online 2008 August 20. doi: 10.1107/S1744309108025384
PMCID:
PMC2531275
Avinash Gill, Dean R. Madden

High-resolution diffraction was obtained from GluR4 AMPA-receptor ligand-binding domain crystals using a combination of cryogenic and room-temperature data-collection strategies.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 831–835. Published online 2008 August 20. doi: 10.1107/S1744309108025426
PMCID:
PMC2531279
Sander H. J. Smits, Andre Mueller, Manfred K. Grieshaber, Lutz Schmitt

The crystal structure of octopine dehydrogenase revealed a specific role of the His5 tag in inducing the crystal contacts required for successful crystallization.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 836–839. Published online 2008 August 20. doi: 10.1107/S1744309108025487
PMCID:
PMC2531259
Sabine Gorynia, Pedro M. Matias, Tiago M. Bandeiras, Peter Donner, Maria Arménia Carrondo

A truncated variant of the human RuvBL1–RuvBL2 complex was cloned, expressed, purified and crystallised. Synchrotron diffraction data to 4 Å resolution were used to carry out a preliminary crystallographic analysis of the complex.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 840–846. Published online 2008 August 20. doi: 10.1107/S174430910802558X
PMCID:
PMC2531268
Shu Wang, Yong-Xing He, Rui Bao, Yan-Bin Teng, Bo-Ping Ye, Cong-Zhao Zhou

A hypothetical protein SCO4226 from S. coelicolor has been overexpressed, purified and crystallized. The crystals belonged to space group P21 and diffracted to 2.0 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 847–850. Published online 2008 August 20. doi: 10.1107/S174430910802575X
PMCID:
PMC2531269
Yasufumi Ueda, Naoki Shibata, Daisuke Takeuchi, Masaya Kitamura, Yoshiki Higuchi

Flavoredoxin from D. vulgaris Miyazaki F has been purified and crystallized. The crystals diffracted to 1.05 Å resolution using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 851–853. Published online 2008 August 20. doi: 10.1107/S1744309108025840
PMCID:
PMC2531261
Ayman M. Awama, Patricia Paracuellos, Sabine Laurent, Colette Dissous, Olivier Marcillat, Patrice Gouet

The X-ray structure of the guanidino kinase from S. mansoni has been determined at 2.8 Å resolution by the molecular-replacement method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 854–857. Published online 2008 August 20. doi: 10.1107/S1744309108025979
PMCID:
PMC2531260
Hironari Shimizu, Coh-ichi Nihei, Daniel Ken Inaoka, Tatushi Mogi, Kiyoshi Kita, Shigeharu Harada

Succinate:ubiquinone oxidoreductase was solubilized and purified from E. coli inner membranes using several different detergents and the phospholipid content of each preparation was analyzed. The preparation with the lowest phospholipid content crystallized in two different crystal forms using detergent mixtures composed of n-alkyl-oligoethylene glycol monoether and n-alkyl-maltoside.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 858–862. Published online 2008 August 29. doi: 10.1107/S1744309108026596
PMCID:
PMC2531281
Linda Schuldt, Simone Weyand, Georgia Kefala, Manfred S. Weiss

M. tuberculosis tetrahydrodipicolinate-N-succinyltransferase, the enzyme that catalyses the fifth reaction step of the lysine-biosynthesis pathway, has been cloned, expressed, purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 863–866. Published online 2008 August 29. doi: 10.1107/S1744309108026559
PMCID:
PMC2531272
Takashi Oda, Hiroshi Hashimoto, Naoyuki Kuwabara, Kokoro Hayashi, Chojiro Kojima, Tsutomu Kawasaki, Ko Shimamoto, Mamoru Sato, Toshiyuki Shimizu

A crystal of the N-terminal domain of a plant NADPH oxidase was obtained and X-ray diffraction data were collected on a synchrotron beamline to a maximum resolution of 2.4 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 867–869. Published online 2008 August 29. doi: 10.1107/S1744309108026535
PMCID:
PMC2531284
Miroslaw Tarnawski, Szymon Krzywda, Andrzej Szczepaniak, Mariusz Jaskolski

Recombinant RuBisCO chaperone from T. elongatus, TeRbcX, was aggregated and could not be crystallized. Mutations of an unusual Cys residue in the TeRbcX sequence yielded much better behaved proteins that could rapidly be crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 September 1; 64(Pt 9): 870–874. Published online 2008 August 29. doi: 10.1107/S174430910802678X
PMCID:
PMC2531263

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography