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Volume 63(Pt 7);  2007 July 1

Protein Structure Communications

Andy Maraite, Thomas Schmidt, Marion B. Ansörge-Schumacher, A. Marek Brzozowski, Gideon Grogan

The X-ray crystal structure of the ThDP-dependent enzyme benzaldehyde lyase has been refined to 1.65 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 546–548. Published online 2007 June 15. doi: 10.1107/S1744309107028576
PMCID:
PMC2335142

Structural Genomics Communications

Laura-Lee Clancy Kelley, Bret D. Dillard, Wolfram Tempel, Lirong Chen, Neil Shaw, Doowon Lee, M. Gary Newton, Frank J. Sugar, Francis E. Jenney, Jr, Han Seung Lee, Claudia Shah, Farris L. Poole, III, Michael W. W. Adams, Jane S. Richardson, David C. Richardson, Zhi-Jie Liu, Bi-Cheng Wang, John Rose

The crystal structure of the hypothetical protein PF0899 from P. furiosus has been determined to 1.85 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 549–552. Published online 2007 June 11. doi: 10.1107/S1744309107024049
PMCID:
PMC2335137

Crystallization Communications

Mikael Elias, Jérôme Dupuy, Luigia Merone, Claude Lecomte, Mosè Rossi, Patrick Masson, Giuseppe Manco, Eric Chabriere

A phosphotriesterase (PTE) from the hyperthermophilic archaeon S. solfataricus has been crystallized. Combined with biochemical and bioengineering studies, it is expected that the structure of this protein will provide insight into the natural function of the PTE family and provide important data for achieving an efficient organophosphate biodecontaminant.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 553–555. Published online 2007 June 11. doi: 10.1107/S1744309107023512
PMCID:
PMC2335143
Seong-Hoon Kim, Hideyuki Miyatake, Tamao Hisano, Wakana Iwasaki, Akio Ebihara, Kunio Miki

Crystals of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase from T. thermophilus HB8 were obtained which diffracted synchrotron X-rays to a resolution of 1.6 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 556–559. Published online 2007 June 11. doi: 10.1107/S174430910702492X
PMCID:
PMC2335138
Kiho Seike, Junji Sato, Koji Tomoo, Toshimasa Ishida, Akihito Yamano, Sadao Ikenishi, Katsushiro Miyamoto, Hiroshi Tsujibo

To clarify the structural basis of sugar binding by BxlE at the atomic level, recombinant BxlE was crystallized using the hanging-drop vapour-diffusion method at 290 K.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 560–562. Published online 2007 June 11. doi: 10.1107/S1744309107024906
PMCID:
PMC2335139
Blanca de las Rivas, Héctor Rodríguez, Iván Angulo, Rosario Muñoz, José M. Mancheño

The catabolic ornithine transcarbamylase (cOTC) from L. hilgardii has been overexpressed in E. coli, purified and crystallized under two different experimental conditions. The structure has been solved by the molecular-replacement method using the atomic coordinates of catabolic ornithine transcarbamylase from P. aeruginosa as the search model.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 563–567. Published online 2007 June 11. doi: 10.1107/S1744309107025195
PMCID:
PMC2335135
María José Sánchez-Barrena, Sandra Moreno-Pérez, Iván Angulo, Martín Martínez-Ripoll, Armando Albert

Recombinant SOS3 and SOS2 regulatory domain from A. thaliana have been coexpressed in E. coli, purified and crystallized by the hanging-drop vapour-diffusion method. An X-ray data set has been collected at 2.0 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 568–570. Published online 2007 June 11. doi: 10.1107/S174430910702533X
PMCID:
PMC2335134
Julie Wolfová, Jeroen R. Mesters, Jiří Brynda, Rita Grandori, Antonino Natalello, Jannette Carey, Ivana Kutá Smatanová

E. coli WrbA, the founding member of a novel flavoprotein family, was crystallized in complex with its physiological cofactor. Preliminary diffraction analysis is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 571–575. Published online 2007 June 11. doi: 10.1107/S1744309107026103
PMCID:
PMC2335133
Hiroyuki Morita, Shin Kondo, Ryohei Kato, Kiyofumi Wanibuchi, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe, Toshiyuki Kohno

An acridone-producing novel type III polyketide synthase from H. serrata has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 2.0 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 576–578. Published online 2007 June 11. doi: 10.1107/S1744309107026164
PMCID:
PMC2335141
SeungBum Kim, Sangbum Joo, Hyun C. Yoon, Yeonwoo Ryu, Kyeong Kyu Kim, T. Doohun Kim

Est25, a ketoprofen-specific hormone-sensitive lipase from a metagenomic library, was crystallized and diffraction data were collected to 1.49 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 579–581. Published online 2007 June 11. doi: 10.1107/S1744309107026152
PMCID:
PMC2335126
Mikalai Lapkouski, Santosh Panjikar, Ivana Kuta Smatanova, Eva Csefalvay

The purification, crystallization and preliminary diffraction analysis of the HsdR subunit of the EcoR124I endonuclease are described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 582–585. Published online 2007 June 11. doi: 10.1107/S174430910702622X
PMCID:
PMC2335136
Rie Omi, Keiji Jitsumori, Takahiro Yamauchi, Susumu Ichiyama, Tatsuo Kurihara, Nobuyoshi Esaki, Nobuo Kamiya, Ken Hirotsu, Ikuko Miyahara

A recombinant form of dl-2-haloacid dehalogenase from Methylobacterium sp. CPA1 has been expressed in E. coli, purified and crystallized. The crystal belongs to space group P63. Diffraction data have been collected to 1.75 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 586–589. Published online 2007 June 15. doi: 10.1107/S1744309107027273
PMCID:
PMC2335131
Sebastien Moniot, Mikael Elias, Donghyo Kim, Ken Scott, Eric Chabriere

Crystallization of DING protein from P. fluorescens is reported. A complete data set was collected to 1.43 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 590–592. Published online 2007 June 15. doi: 10.1107/S1744309107028102
PMCID:
PMC2335140
Yanshi Zhu, Isobel Black, Aleksander W. Roszak, Neil W. Isaacs

P30, the transmembrane C-terminal domain of pertactin from B. pertussis has been crystallized after refolding in vitro. Preliminary X-ray crystallographic data are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 593–595. Published online 2007 June 15. doi: 10.1107/S1744309107028308
PMCID:
PMC2335132
Rosicler Lázaro Barbosa, Fábio Cupri Rinaldi, Beatriz Gomes Guimarães, Celso Eduardo Benedetti

In order to gain new insights into the protein structure and its possible interaction with a metal ion or effector ligand, BigR from X. fastidiosa was crystallized in native and selenomethionine (SeMet) labelled forms using the hanging-drop vapour-diffusion method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 596–598. Published online 2007 June 15. doi: 10.1107/S1744309107028722
PMCID:
PMC2335149
Pengyun Li, Tanya Rogers, David Smiley, Richard D. DiMarchi, Faming Zhang

The synthesis and crystallization of glucagon-Cex are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 599–601. Published online 2007 June 15. doi: 10.1107/S1744309107028655
PMCID:
PMC2335127
Michiko Kitano-Takahashi, Hiroyuki Morita, Shin Kondo, Kayoko Tomizawa, Ryohei Kato, Michikazu Tanio, Yoshiko Shirota, Hiroshi Takahashi, Shigetoshi Sugio, Toshiyuki Kohno

The kinase domain (residues 1–331) of human tau-tubulin kinase 2 was expressed in insect cells, purified and crystallized. Diffraction data have been collected to 2.9 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 602–604. Published online 2007 June 15. doi: 10.1107/S1744309107028783
PMCID:
PMC2335129
Mário T. Murakami, Ulrich Kuch, Dietrich Mebs, Raghuvir K. Arni

A single crystal of zhaoermiatoxin with maximum dimensions of 0.2 × 0.2 × 0.5 mm was used for X-ray diffraction data collection to a resolution of 2.05 Å using synchrotron radiation and the diffraction pattern was indexed in the hexagonal space group P64, with unit-cell parameters a = 72.9, b = 72.9, c = 93.9 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 605–607. Published online 2007 June 22. doi: 10.1107/S1744309107026073
PMCID:
PMC2335147
Kaoru Suzuki, Yoshiteru Sato, Yohei Maeda, Satoru Shimizu, Md Tofazzal Hossain, Souichirou Ubukata, Takeshi Sekiguchi, Akio Takénaka

A putative nondiscriminating aspartyl-tRNA synthetase from the crenarchaeon S. tokodaii strain 7 has been recombinantly expressed, purified and crystallized. The crystal structure has been preliminarily solved at 2.3 Å resolution by the molecular-replacement method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 608–612. Published online 2007 June 22. doi: 10.1107/S1744309107026905
PMCID:
PMC2335148
Takashi Umehara, Masatoshi Wakamori, Akiko Tanaka, Balasundaram Padmanabhan, Shigeyuki Yokoyama

The C-terminal bromodomain of human BRD2 was cloned, expressed, purified and crystallized. A complete diffraction data set has been collected to 1.80 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 613–615. Published online 2007 June 22. doi: 10.1107/S1744309107028473
PMCID:
PMC2335128
Yusuke Sato, Shuya Fukai, Osamu Nureki

Crystal structure of a triple mutant (M115L, K121M, T142M) of SeMet-labelled Sec2p, a GEF for Rab GTPase, has been solved by SAD. Control of the selenium sites by mutagenesis much improved the resolution of the SeMet-labelled crystals.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 616–619. Published online 2007 June 22. doi: 10.1107/S1744309107027121
PMCID:
PMC2335144
Karthik K. Shanmuganatham, Manimekalai Ravichandran, Martha M. Howe, Hee-Won Park

The isolation and preliminary X-ray analysis of crystals of phage Mu activator protein C bound to promoter DNA are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 620–623. Published online 2007 June 22. doi: 10.1107/S1744309107025286
PMCID:
PMC2335125
Xuhua Tang, Choy Leong Hew

The crystallization of the N-terminal transmembrane region-truncated VP26 and VP28 of white spot syndrome virus is described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 624–626. Published online 2007 June 22. doi: 10.1107/S1744309107029351
PMCID:
PMC2335124
Takanori Muto, Daisuke Tsuchiya, Kosuke Morikawa, Hisato Jingami

The ligand-binding domain of metabotropic glutamate receptor 7 has been overexpressed, purified, and crystallized by the hanging-drop vapour-diffusion method. A complete data set has been collected to 3.30 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 627–630. Published online 2007 June 29. doi: 10.1107/S1744309107030059
PMCID:
PMC2335145
Pravin Kumar, Ardeschir Vahedi-Faridi, Elena Merino, José A. López de Castro, Armin Volz, Andreas Ziegler, Wolfram Saenger, Barbara Uchanska-Ziegler

The crystallization of HLA-B*1402 in complex with two peptides is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 July 1; 63(Pt 7): 631–634. Published online 2007 June 29. doi: 10.1107/S1744309107029077
PMCID:
PMC2335130

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography