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Volume 63(Pt 6);  2007 June 1

Protein Structure Communications

Philip D. Kiser, David T. Lodowski, Krzysztof Palczewski

A 3.02 Å crystal structure of native GroEL from E. coli is presented.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 457–461. Published online 2007 May 5. doi: 10.1107/S1744309107020295
PMCID:
PMC2335072
Eiji Inagaki, Noriyasu Ohshima, Keiko Sakamoto, Nigar D. Babayeva, Hiroaki Kato, Shigeyuki Yokoyama, Tahir H. Tahirov

A crystal structure of NADP+-bound T. thermophilus Δ1-pyrroline-5-carboxylate dehydrogenase refined to 1.55 Å resolution is reported. The structure provides structural insights into the mechanism of preference for coenzymes and enzyme activity.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 462–465. Published online 2007 May 5. doi: 10.1107/S1744309107021422
PMCID:
PMC2335078
Shunsuke Yajima, Kodai Hara, Daisuke Iino, Yasuyuki Sasaki, Tomohisa Kuzuyama, Kanju Ohsawa, Haruo Seto

The crystal structure of 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR) from Escherichia coli complexed with Mg2+, NADPH and fosmidomycin was determined at 2.2 Å resolution. The structure showed a well defined loop conformation at the active site of DXR.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 466–470. Published online 2007 May 31. doi: 10.1107/S1744309107024475
PMCID:
PMC2335089
Masakazu Sugishima, Kenji Oda, Takashi Ogura, Hiroshi Sakamoto, Masato Noguchi, Keiichi Fukuyama

Alternative cyanide-binding modes in the haem–haem oxygenase complex are described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 471–474. Published online 2007 May 31. doi: 10.1107/S174430910702475X
PMCID:
PMC2335079

Crystallization Communications

Midori Murakami, Rei Kitahara, Toshiaki Gotoh, Tsutomu Kouyama

Truncated rhodopsin from the retina of the squid Todarodes pacificus was extracted and crystallized by the sitting-drop vapour-diffusion method. Hexagonal crystals grown in the presence of octylglucoside and ammonium sulfate diffracted to 2.8 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 475–479. Published online 2007 May 5. doi: 10.1107/S1744309107017423
PMCID:
PMC2335087
Beverly E. Prosser, Steven Johnson, Pietro Roversi, Simon J. Clark, Edward Tarelli, Robert B. Sim, Antony J. Day, Susan M. Lea

The crystallization of human complement regulator FH-678402H with a glycosaminoglycan analogue is described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 480–483. Published online 2007 May 5. doi: 10.1107/S1744309107020052
PMCID:
PMC2335081
Abhijit A. Gurjar, Neela H. Yennawar, Hemant P. Yennawar, Kanagalaghatta R. Rajashankar, Narasimha V. Hegde, Bhushan M. Jayarao

The cloning, expression, purification and crystallization of recombinant Clostridium perfringens β2-toxin is described. The crystals diffracted to 2.9 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 484–487. Published online 2007 May 5. doi: 10.1107/S1744309107020313
PMCID:
PMC2335086
Jaldappagari Seetharamappa, Muse Oke, Huanting Liu, Stephen A. McMahon, Kenneth A. Johnson, Lester Carter, Mark Dorward, Michal Zawadzki, Ian M. Overton, C. A. Johannes van Niekirk, Shirley Graham, Catherine H. Botting, Garry L. Taylor, Malcolm F. White, Geoffrey J. Barton, Peter J. Coote, James H. Naismith

Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 488–491. Published online 2007 May 5. doi: 10.1107/S1744309107020362
PMCID:
PMC2335074
Chung-Der Chen, Tien-Feng Huang, Chih-Hao Lin, Hong-Hsiang Guan, Yin-Cheng Hsieh, Yi-Hung Lin, Yen-Chieh Huang, Ming-Yih Liu, Wen-Chang Chang, Chun-Jung Chen

The crystallization of branched-chain aminotransferase from D. radiodurans is described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 492–494. Published online 2007 May 5. doi: 10.1107/S1744309107020842
PMCID:
PMC2335077
Ilham Jabafi, Barbara Selisko, Bruno Coutard, Armando M. De Palma, Johan Neyts, Marie-Pierre Egloff, Sacha Grisel, Karen Dalle, Valerie Campanacci, Silvia Spinelli, Christian Cambillau, Bruno Canard, Arnaud Gruez

The first crystal of a coxsackievirus RNA-dependent RNA polymerase is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 495–498. Published online 2007 May 12. doi: 10.1107/S1744309107020416
PMCID:
PMC2335076
Yuji Ashikawa, Hiromasa Uchimura, Zui Fujimoto, Kengo Inoue, Haruko Noguchi, Hisakazu Yamane, Hideaki Nojiri

The NAD(P)H:ferredoxin oxidoreductase in carbazole 1,9a-dioxygenase from Janthinobacterium sp. J3 was crystallized and diffraction data were collected to 2.60 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 499–502. Published online 2007 May 12. doi: 10.1107/S174430910702163X
PMCID:
PMC2335075
Daniela Bumbaca, Jeffrey Kosman, Peter Setlow, R. Keith Henderson, Mark J. Jedrzejas

An α/β-type small, acid-soluble spore protein (SASP) from Bacillus subtilis, a major source of DNA protection against damaging effects in spores, was crystallized in a functionally relevant complex with a double-stranded DNA. This report provides insights into initial characterization of the complex and its structure elucidation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 503–506. Published online 2007 May 12. doi: 10.1107/S1744309107022750
PMCID:
PMC2335083
Wilko Rauert, Ali Nasser Eddine, Stefan H. E. Kaufmann, Manfred S. Weiss, Robert Janowski

The M. tuberculosis protein Rv0765c was cloned, expressed, purified and crystallized. In an attempt to improve the quality of the crystals of Rv0765c, the protein was modified by reductive methylation. The methylated protein crystallized in a new crystal form with profoundly improved diffraction properties.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 507–511. Published online 2007 May 12. doi: 10.1107/S1744309107022506
PMCID:
PMC2335070
Chinthalapudi Krishna, Ruchi Jain, Tara Kashav, Dinakar Wadhwa, Neelima Alam, S. Gourinath

A preliminary crystallographic study of cysteine synthase, a major enzyme in the cysteine-biosynthesis pathway, from the amoebic pathogen E. histolytica.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 512–515. Published online 2007 May 12. doi: 10.1107/S1744309107022154
PMCID:
PMC2335080
Catarina Coelho, Pablo J. González, José Trincão, Ana L. Carvalho, Shabir Najmudin, Thomas Hettman, Stephan Dieckman, José J. G. Moura, Isabel Moura, Maria J. Romão

Crystals of the oxidized form of the periplasmic nitrate reductase from Cupriavidus necator were obtained using polyethylene glycol 3350 as precipitant

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 516–519. Published online 2007 May 12. doi: 10.1107/S1744309107022129
PMCID:
PMC2335073
Miki Senda, Shinya Kishigami, Shigenobu Kimura, Toshiya Senda

The electron-transfer complex of BphA3, a Rieske-type [2Fe–2S] ferredoxin, and BphA4, a NADH-dependent ferredoxin reductase, was crystallized by the sitting-drop vapour-diffusion method under anaerobic conditions.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 520–523. Published online 2007 May 18. doi: 10.1107/S1744309107023007
PMCID:
PMC2335082
Catherine F. Latham, Shu-Hong Hu, Christine L. Gee, Chris J. Armishaw, Paul F. Alewood, David E. James, Jennifer L. Martin

Cocrystallization with a peptide, free-interface diffusion crystal chips and crystal dehydration were important in the production of diffraction-quality crystals of the Munc18c protein that helps to regulate membrane fusion.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 524–528. Published online 2007 May 18. doi: 10.1107/S1744309107022361
PMCID:
PMC2335071
Naoki Shibata, Yusuke Tomimoto, Toru Hanamura, Ryo Yamamoto, Mai Ueda, Yasufumi Ueda, Nobuhiro Mizuno, Hideaki Ogata, Hirofumi Komori, Yasuhito Shomura, Michihiko Kataoka, Sakayu Shimizu, Jun Kondo, Hideki Yamamoto, Akira Kikuchi, Yoshiki Higuchi

The DIX domain of rat axin has been purified and crystallized. Crystals diffracted to 2.9 Å resolution using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 529–531. Published online 2007 May 18. doi: 10.1107/S1744309107022579
PMCID:
PMC2335085
Michio Shirokane, Yoriko Sawano, Ken-ichi Miyazono, Koji Nagata, Masaru Tanokura

PH1010, a DUF54-family protein from the hyperthermophilic archaeon P. horikoshii OT3, was crystallized and X-ray diffraction data were collected to 1.90 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 532–534. Published online 2007 May 31. doi: 10.1107/S1744309107024487
PMCID:
PMC2335084
Fumiaki Yumoto, Koji Nagata, Yumiko Miyauchi, Takao Ojima, Hiroyuki Tanaka, Kiyoyoshi Nishita, Iwao Ohtsuki, Masaru Tanokura

Recombinant TnC was expressed in Escherichia coli, purified, complexed with a 24-residue synthetic peptide derived from scallop troponin I (TnI) and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 535–537. Published online 2007 May 31. doi: 10.1107/S1744309107024712
PMCID:
PMC2335068
Satoshi Watanabe, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

The [NiFe] hydrogenase maturation proteins HypC and HypD were purified and crystallized. Crystals of HypC and HypD suitable for data collection diffracted to 1.80 and 2.07 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 538–541. Published online 2007 May 31. doi: 10.1107/S1744309107023391
PMCID:
PMC2335088
E. B. Struble, A. G. Gittis, M. A. Bianchet, R. McMacken

Crystallization and preliminary diffraction data of the N-terminal 19–139 fragment of the origin-binding domain of bacteriophage λ O replication initiator are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 June 1; 63(Pt 6): 542–545. Published online 2007 May 31. doi: 10.1107/S1744309107022762
PMCID:
PMC2335069

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography