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Volume 63(Pt 4);  2007 April 1

Protein Structure Communications

Rajesh Kumar Singh, Gottfried J. Palm, Santosh Panjikar, Winfried Hinrichs

Crystal structure analysis of the apo form of catabolite control protein A reveals the three-helix bundle of the DNA-binding domain. In the crystal packing, this domain interacts with the binding site for the corepressor protein.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 253–257. Published online 2007 March 12. doi: 10.1107/S1744309107008949
PMCID:
PMC2330204
Abdul S. Ethayathulla, Devendra B. Srivastava, Janesh Kumar, Kolandaivelu Saravanan, Sameeta Bilgrami, Sujata Sharma, Punit Kaur, Alagiri Srinivasan, Tej P. Singh

The crystal structure of a signalling glycoprotein isolated from buffalo dry secretions (SPB-40) has been determined at 2.8 Å resolution. Two unique residues, Tyr120 and Glu269, found in SPB-40 distort the shape of the sugar-binding groove considerably. The water structure in the groove is also different. The conformations of three flexible loops, His188–His197, Phe202–Arg212

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 258–265. Published online 2007 March 12. doi: 10.1107/S1744309107010445
PMCID:
PMC2330205
A. Shaw, M. L. Saldajeno, M. A. B. Kolkman, B. E. Jones, R. Bott

The crystal structure of a secreted chymotrypsin from the alkalophile Cellulomonas bogoriensis has been determined using data to 1.78 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 266–269. Published online 2007 March 23. doi: 10.1107/S1744309107008937
PMCID:
PMC2330206
Tine Kragh Nielsen, Christian Hildmann, Daniel Riester, Dennis Wegener, Andreas Schwienhorst, Ralf Ficner

The crystal structure of HDAH FB188 in complex with a trifluoromethylketone at 2.2 Å resolution is reported and compared to a previously determined inhibitor complex.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 270–273. Published online 2007 March 23. doi: 10.1107/S1744309107012377
PMCID:
PMC2330214

Structural Genomics Communications

Sangwoo Kim, Michihiro Suga, Kyoko Ogasahara, Terumi Ikegami, Yoshiko Minami, Toshitsugu Yubisui, Tomitake Tsukihara

The structure of P. polycephalum cytochrome b 5 reductase, an enzyme which catalyzes the reduction of cytochrome b 5 by NADH, was determined at a resolution of 1.56 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 274–279. Published online 2007 March 23. doi: 10.1107/S1744309107010731
PMCID:
PMC2330227

Crystallization Communications

Ju Sheng, Jun Ye, Barry P. Rosen

Glutaredoxin 2 from E. coli was cocrystallized with glutathione and data were collected to 1.60 Å. A mutant with the active-site residues Cys9 and Cys12 changed to serine was crystallized in the absence of glutathione and data were collected to 2.4 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 280–282. Published online 2007 March 12. doi: 10.1107/S1744309107004204
PMCID:
PMC2330212
Bernardo Rodamilans, Guillermo Montoya

Crystals of DDX3 RNA helicase domain have been obtained in a monoclinic form that diffract to 2.2 Å resolution using synchrotron radiation at the ID14-1 ESRF beamline.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 283–286. Published online 2007 March 12. doi: 10.1107/S1744309107006434
PMCID:
PMC2330201
K. F. Santos, M. T. Murakami, A. C. O. Cintra, M. H. Toyama, S. Marangoni, V. P. Forrer, J. R. Brandão Neto, I. Polikarpov, R. K. Arni

Crotoxin, a potent neurotoxin from the venom of the South American rattlesnake Crotalus durissus terrificus, exists as a heterodimer formed between a phospholipase A2 and a catalytically inactive acidic phospholipase A2 analogue (crotapotin). Large single crystals of the crotoxin complex and of the isolated subunits have been obtained.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 287–290. Published online 2007 March 12. doi: 10.1107/S1744309107006719
PMCID:
PMC2330203
Adeleke Halilu Aguda, Amos Malle Sakwe, Lars Rask, Robert Charles Robinson

The crystallization and crystallographic data analysis of filamin repeats 14–16 are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 291–293. Published online 2007 March 12. doi: 10.1107/S1744309107006689
PMCID:
PMC2330200
Yukari Sato, Ryo Natsume, Masataka Tsuda, Jiri Damborsky, Yuji Nagata, Toshiya Senda

A haloalkane dehalogenase, DbjA, was crystallized by the hanging-drop vapour-diffusion method using PEG 4000 as a precipitant. The crystal belongs to the orthorhombic system, space group P21212 and diffracts to 1.75 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 294–296. Published online 2007 March 12. doi: 10.1107/S1744309107008652
PMCID:
PMC2330215
Takashi Mukasa, Eugenio Santelli, John C. Reed, Jaime Pascual

A leucine-zipper with properties as apoptotic regulator in the ER has been crystallized. X-ray data to 2.5 Å resolution were collected, molecular replacement solutions were identified and refinement has been started.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 297–299. Published online 2007 March 12. doi: 10.1107/S1744309107008925
PMCID:
PMC2330218
Héctor Rodríguez, Blanca de las Rivas, Rosario Muñoz, José M. Mancheño

The enzyme p-coumaric acid decarboxylase (PDC) from L. plantarum has been recombinantly expressed, purified and crystallized. The structure has been solved at 2.04 Å resolution by the molecular-replacement method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 300–303. Published online 2007 March 12. doi: 10.1107/S1744309107008846
PMCID:
PMC2330202
Andrew A. McCarthy, Laurent Biget, Chenwei Lin, Vincent Petiard, Steve D. Tanksley, James G. McCarthy

The genes encoding XMT and DXMT, the enzymes from Coffea canephora (robusta) that catalyse the three independent N-methyl transfer reactions in the caffeine-biosynthesis pathway, have been cloned and the proteins have been expressed in Escherichia coli. Both proteins have been crystallized in the presence of the demethylated cofactor S-adenosyl-l-cysteine (SAH) and substrate (xanthosine for XMT and

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 304–307. Published online 2007 March 12. doi: 10.1107/S1744309107009268
PMCID:
PMC2330209
Katsumi Shimizu, Naoki Kunishima

The pyrimidine nucleoside phosphorylase TTHA1771 from T. thermophilus HB8 has been overexpressed, purified and crystallized. The crystals diffract X-rays to 1.8 Å resolution using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 308–310. Published online 2007 March 12. doi: 10.1107/S1744309107009980
PMCID:
PMC2330216
Miki Senda, Shinya Kishigami, Shigenobu Kimura, Toshiya Senda

The reduced form of BphA3, a Rieske-type [2Fe–2S] ferredoxin, was crystallized by the sitting-drop vapour-diffusion method under anaerobic conditions. A molecular-replacement calculation yielded a satisfactory solution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 311–314. Published online 2007 March 12. doi: 10.1107/S1744309107009992
PMCID:
PMC2330217
Takayoshi Kinoshita, Taro Tamada, Keisuke Imai, Kazuo Kurihara, Takashi Ohhara, Toshiji Tada, Ryota Kuroki

To investigate the structural characteristics of a covalent inhibitor bound to porcine pancreatic elastase (PPE), including H atoms and hydration by water, a crystal of porcine pancreatic elastase with its inhibitor was grown to a size of 1.6 mm3 for neutron diffraction study. The crystal diffracted to 2.3 Å resolution with sufficient quality for further structure determination

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 315–317. Published online 2007 March 12. doi: 10.1107/S1744309107010433
PMCID:
PMC2330211
Chandan Shee, Tej P. Singh, Pravindra Kumar, Ashwani K. Sharma

A Kunitz-type trypsin inhibitor purified from the seeds of Murraya koenigii has been crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as the precipitating agent.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 318–319. Published online 2007 March 23. doi: 10.1107/S1744309107011414
PMCID:
PMC2330219
Roxana M. Coman, Arthur Robbins, Maureen M. Goodenow, Robert McKenna, Ben M. Dunn

Crystals of the human immunodeficiency virus 1 subtype C protease complexed with indinavir and nelfinavir have been grown in the monoclinic space group P21 and shown to diffract X-rays to 2.3 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 320–323. Published online 2007 March 30. doi: 10.1107/S174430910701161X
PMCID:
PMC2330223
Shankar Prasad Kanaujia, Chellamuthu Vasuki Ranjani, Jeyaraman Jeyakanthan, Miwa Ohmori, Kazuko Agari, Yoshiaki Kitamura, Seiki Baba, Akio Ebihara, Akeo Shinkai, Seiki Kuramitsu, Yoshitsugu Shiro, Kanagaraj Sekar, Shigeyuki Yokoyama

The molybdopterin synthase from T. thermophilus HB8 was cloned, expressed, purified and crystallized. The crystals belong to space group P21 and diffracted to a resolution of 1.64 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 324–326. Published online 2007 March 30. doi: 10.1107/S1744309107011426
PMCID:
PMC2330224
Hua Ming, Ken-ichi Miyazono, Masaru Tanokura

Preliminary crystallographic analysis of KaiC-like protein PH0186 from Pyrococcus horikoshii OT3 is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 327–329. Published online 2007 March 30. doi: 10.1107/S1744309107011402
PMCID:
PMC2330226
Nathalie Troffer-Charlier, Vincent Cura, Pierre Hassenboehler, Dino Moras, Jean Cavarelli

Isolated modules of mouse coactivator-associated arginine methyltransferase 1 encompassing the protein arginine N-methyltransferase catalytic domain have been overexpressed, purified and crystallized. X-ray diffraction data have been collected and have enabled determination of the structures by multiple isomorphous replacement using anomalous scattering.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 330–333. Published online 2007 March 30. doi: 10.1107/S1744309107011785
PMCID:
PMC2330207
Bagautdin Bagautdinov, Yoshinori Matsuura, Svetlana Bagautdinova, Naoki Kunishima

A truncated form of biotin carboxyl carrier protein containing the C-terminal half fragment (BCCPΔN76) and the biotin protein ligase (BPL) with the mutation R48A (BPL*) or the double mutation R48A K111A (BPL**) were successfully cocrystallized in the presence of ATP and biotin. The BPL*–BCCPΔN76 and BPL**–BCCPΔN76 crystals belong to space group P21 and diffract

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 334–337. Published online 2007 March 30. doi: 10.1107/S1744309107011967
PMCID:
PMC2330208
Luc Bonnefond, Magali Frugier, Elodie Touzé, Bernard Lorber, Catherine Florentz, Richard Giegé, Joëlle Rudinger-Thirion, Claude Sauter

Crystals of human mitochondrial tyrosyl-tRNA synthetase lacking the C-terminal S4-like domain diffract to 2.7 Å resolution and are suitable for structure determination.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 338–341. Published online 2007 March 30. doi: 10.1107/S1744309107012481
PMCID:
PMC2330213
Xiaoyun Pang, Feng Xu, Stephen G. Bell, Delin Guo, Luet-Lok Wong, Zihe Rao

The cytochrome P450 enzyme CYP203A1 from Rhodopseudomonas palustris binds a wide range of highly substituted aromatic compounds and may play an important role in the astonishing metabolic diversity of this organism. Crystals of CYP203A1 that diffract to 2.0 Å resolution have been obtained.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 342–345. Published online 2007 March 30. doi: 10.1107/S1744309107012705
PMCID:
PMC2330210
Akira Nakamura, Chieko Wada, Kunio Miki

A dimeric inert form of the replication initiator RepE of the F plasmid was expressed, purified and crystallized in complex with the repE operator DNA. Diffraction data were collected to 3.14 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 346–349. Published online 2007 March 30. doi: 10.1107/S1744309107012894
PMCID:
PMC2330221
Jennifer Puttick, Claire Vieille, Seung H. Song, Michel N. Fodje, Pawel Grochulski, Louis T. J. Delbaere

T. maritima mannitol dehydrogenase has been crystallized in space group P212121 with a = 84.43, b = 120.61, c = 145.76 Å. The crystals diffracted to 3.3 Å resolution at the Canadian Light Source.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 350–352. Published online 2007 March 30. doi: 10.1107/S1744309107013097
PMCID:
PMC2330225
Divya Mathur, Kanchan Anand, Deepika Mathur, Nirmala Jagadish, Anil Suri, Lalit C. Garg

The phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv was crystallized and diffraction data were collected to 2.8 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 353–355. Published online 2007 March 30. doi: 10.1107/S1744309107013218
PMCID:
PMC2330222
Natalie De Jonge, Lieven Buts, Joris Vangelooven, Natacha Mine, Laurence Van Melderen, Lode Wyns, Remy Loris

A CcdB homologue from V. fischeri was overexpressed in E. coli and purified. The free protein was crystallized, as were its complexes with fragments of E. coli and V. fischeri gyrase and with the F-plasmid CcdA C-terminal domain.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 April 1; 63(Pt 4): 356–360. Published online 2007 March 30. doi: 10.1107/S1744309107012092
PMCID:
PMC2330220

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography