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Volume 61(Pt 4);  2005 April 1

Protein Structure Communications

Maria V. Dontsova, Azat G. Gabdoulkhakov, Olga K. Molchan, Alexandr A. Lashkov, Maria B. Garber, Alexandr S. Mironov, Nadegda E. Zhukhlistova, Ekaterina Yu. Morgunova, Wolfgang Voelter, Christian Betzel, Yang Zhang, Steven E. Ealick, Al’bert M. Mikhailov

S. typhimurium uridine phosphorylase is a homohexamer and is structurally homologous to the hexameric purine nucleoside phosphorylases.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 337–340. Published online 2005 March 24. doi: 10.1107/S1744309105007463
PMCID:
PMC1952443
Nallini Vijayarangan Adikesavan, Syed Saad Mahmood, Nithianantham Stanley, Zhen Xu, Nan Wu, Marc Thibonnier, Menachem Shoham

The 1.8 Å crystal structure of an MBP-fusion protein with the C-terminal cytoplasmic segment of the V1 vasopressin receptor reveals that the receptor segment is unstructured.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 341–345. Published online 2005 March 24. doi: 10.1107/S1744309105007293
PMCID:
PMC1952416
Takayoshi Kinoshita, Riyo Maruki, Masaichi Warizaya, Hidenori Nakajima, Shintaro Nishimura

A high-resolution structure of human triosephosphate isomerase was obtained from crystals improved by means of the gel-tube method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 346–349. Published online 2005 March 24. doi: 10.1107/S1744309105008341
PMCID:
PMC1952429

Structural Genomics Communications

Allan Matte, Gordon V. Louie, J. Sivaraman, Miroslaw Cygler, Stephen K. Burley

The crystal structure of H. influenzae RsuA has been determined and refined at 1.59 Å resolution. While the overall structures of the individual S4 and catalytic domains are very similar to those of E. coli RsuA, a large conformational change between these two domains observed in H. influenzae RsuA offers insight into the possible mode of rRNA recognition by this enzyme.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 350–354. Published online 2005 March 12. doi: 10.1107/S1744309105005920
PMCID:
PMC1952432

Crystallization Communications

Jing Hou, Ming Li, Jiashu Chen, Pengxin Qiu, Xiuxia Liang, Zhiyong Lou, Zihe Rao, Guangmei Yan

Crystals of a non-haemorrhagic fibrin(ogen)olytic metalloproteinase from the venom of A. acutus have been obtained and characterized by X-ray diffraction.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 355–358. Published online 2005 March 12. doi: 10.1107/S1744309105005695
PMCID:
PMC1952413
Doreen Dobritzsch, Birgit Andersen, Jure Piškur

Dihydropyrimidinase from the yeast S. kluyveri was crystallized by vapour diffusion. The crystals belong to space group P21 (unit-cell parameters a = 91.0, b = 73.0, c = 161.4 Å, β = 91.4°) and diffracted to 2.6 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 359–362. Published online 2005 March 12. doi: 10.1107/S174430910500610X
PMCID:
PMC1952421
Andreas Eckhoff, Joachim Granzin, Thilo Kamphausen, Georg Büldt, Burkhard Schulz, Oliver H. Weiergräber

The crystallization of FKBP42, a multi-domain member of the FK506-binding protein family, from the plant A. thaliana is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 363–365. Published online 2005 March 12. doi: 10.1107/S1744309105006342
PMCID:
PMC1952426
Hajime Sugawara, Tomoyuki Yamaya, Hitoshi Sakakibara

The histidine-containing phosphotransfer protein ZmHP1 from maize was crystallized. The removal of an N-terminal His tag resulted in a remarkable improvement of the diffraction data.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 366–368. Published online 2005 March 12. doi: 10.1107/S1744309105006846
PMCID:
PMC1952433
Bi-Hung Peng, Mark A. White, Gerald A. Campbell, Jebamony J. Robert, J. Ching Lee, Roger B. Sutton

Crystallization and preliminary X-ray diffraction of the ZO-binding domain of human occludin.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 369–371. Published online 2005 March 12. doi: 10.1107/S1744309105007475
PMCID:
PMC1952431
Bernhard Loll, Anna Zawacka, Jacek Biesiadka, Christine Rückert, Armin Volz, Wolfram Saenger, Barbara Uchanska-Ziegler, Andreas Ziegler

The product of the human leukocyte antigen (HLA) gene HLA-B*2703 differs from that of the prototypical subtype HLA-B*2705 by a single amino acid at heavy-chain residue 59 that is involved in anchoring the peptide N-terminus within the A pocket of the molecule. Two B*2703–peptide complexes were crystallized using the hanging-drop vapour-diffusion method using PEG 8000 as a precipitant. A pocket

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 372–374. Published online 2005 March 12. doi: 10.1107/S1744309105007438
PMCID:
PMC1952442
Min-Je Ku, Won-Ho Lee, Ki-hyun Nam, Kyeong-hee Rhee, Ki-Seog Lee, Eunice EunKyung Kim, Myung-Hee Yu, Kwang Yeon Hwang

The tRNA-specific adenosine deaminase from the pathogenic bacteria S. pyogenes has been overexpressed and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 375–377. Published online 2005 March 12. doi: 10.1107/S1744309105007311
PMCID:
PMC1952419
Melanie A. Adams, Christian M. Udell, Gour Pada Pal, Zongchao Jia

The crystallization and preliminary X-ray diffraction analysis of MraZ, formerly known as hypothetical protein YabB, from Escherichia coli K-12 is presented.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 378–380. Published online 2005 March 12. doi: 10.1107/S1744309105007657
PMCID:
PMC1952425
T. S. Girish, B. Gopal

The receptor protein tyrosine phosphatase CRYP-2 has been shown to be an inhibitory factor for the growth of retinal axons in the chick. The cloning, expression, purification and crystallization of the catalytic domain of CRYP-2 are reported here.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 381–383. Published online 2005 March 12. doi: 10.1107/S1744309105007505
PMCID:
PMC1952428
Lei Yin, De-Yu Zhu, Na Yang, Qiu-Hua Huang, Ying Zhang, Da-Cheng Wang

The protein SH3BGRL, containing both SH3-binding and Homer EVH1-binding motifs, has been crystallized using the hanging-drop vapour-diffusion method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 384–386. Published online 2005 March 24. doi: 10.1107/S174430910500730X
PMCID:
PMC1952435
Marcos Antonio de Oliveira, Karen Fulan Discola, Simone Vidigal Alves, João Alexandre Ribeiro Gonçalves Barbosa, Francisco Javier Medrano, Luis Eduardo Soares Netto, Beatriz Gomes Guimarães

Thioredoxin reductase 1 (Trr1) from S. cerevisiae is a component of the thioredoxin system, which is involved in several biological processes, including the reduction of disulfide bonds and response to oxidative stress. The expression, purification, crystallization and preliminary X-ray crystallographic studies of yeast Trr1 are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 387–390. Published online 2005 March 24. doi: 10.1107/S174430910500758X
PMCID:
PMC1952441
Oluwatoyin A. Asojo, Alex Loukas, Mehmet Inan, Rick Barent, Jicai Huang, Brad Plantz, Amber Swanson, Mark Gouthro, Michael M. Meagher, Peter J. Hotez

In order to clarify the structural basis of the pathogenesis-related-1 domain, Na-ASP-1, the first multi-domain ASP from the human hookworm parasite N. americanus, has been crystallized. 2.2 Å resolution data have been collected from a crystal belonging to the monoclinic space group P21.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 391–394. Published online 2005 March 24. doi: 10.1107/S1744309105007748
PMCID:
PMC1952415
Shin-ichi Ichiki, Shota Nakamura, Tadayasu Ohkubo, Yuji Kobayashi, Jun Hasegawa, Susumu Uchiyama, Hirofumi Nishihara, Keiko Mizuta, Yoshihiro Sambongi

Cytochrome c 552 of a moderate thermophile, H. thermoluteolus, was overexpressed in E. coli and crystallized for X-ray diffraction study.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 395–398. Published online 2005 March 24. doi: 10.1107/S1744309105007761
PMCID:
PMC1952422
Kosuke Ito, Masayuki Nakanishi, Woo-Cheol Lee, Hiroshi Sasaki, Shuhei Zenno, Kaoru Saigo, Yukio Kitade, Masaru Tanokura

The crystallization and preliminary X-ray analysis of AzoR (azoreductase) have been performed.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 399–402. Published online 2005 March 24. doi: 10.1107/S1744309105007918
PMCID:
PMC1952434
Celia J. Webby, J. Shaun Lott, Heather M. Baker, Edward N. Baker, Emily J. Parker

Crystals of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from M. tuberculosis have been grown and a native data set has been collected to a maximum resolution of 2.5 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 403–406. Published online 2005 March 24. doi: 10.1107/S1744309105007931
PMCID:
PMC1952427
Linnea E. K. Wikman, Julya Krasotkina, Anastasia Kuchumova, Nikolay N. Sokolov, Anastassios C. Papageorgiou

Er. carotovora l-asparaginase, a potential antileukaemic agent, has been crystallized. Crystals diffract to 2.6 Å using a rotating-anode source and belong to space group P21, with unit-cell parameters a = 78.0, b = 112.3, c = 78.7 Å, β = 101.9° and a homotetramer in the crystallographic asymmetric unit.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 407–409. Published online 2005 March 24. doi: 10.1107/S1744309105008249
PMCID:
PMC1952439
Rupa Walanj, Paul Young, Heather M. Baker, Edward N. Baker, Peter Metcalf, Joseph W. Chow, Stephen Lerner, Sergei Vakulenko, Clyde A. Smith

APH(2′′)-Ib is an enzyme responsible for high-level gentamicin resistance in E. faecium isolates. Native crystals of this enzyme have been prepared and preliminary X-ray diffraction experiments have been undertaken.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 410–413. Published online 2005 April 1. doi: 10.1107/S174430910500775X
PMCID:
PMC1952440
Linyen Lin, Hiroaki Nakano, Susumu Uchiyama, Satoru Fujimoto, Sachihiro Matsunaga, Shota Nakamura, Yuji Kobayashi, Tadayasu Ohkubo, Kiichi Fukui

A plant- and prokaryote-conserved domain (PPC) has been crystallized. The crystal diffracted to 1.7 Å resolution and belonged to space group P6322.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 414–416. Published online 2005 April 1. doi: 10.1107/S1744309105007815
PMCID:
PMC1952417
S. Gourinath, Narendra Padhan, Neelima Alam, Alok Bhattacharya

Calcium-binding protein-2 (EhCaBP2) crystals were grown using MPD as a precipitant. EhCaBP2 also crystallized in complex with strontium (replacing calcium) at similar conditions. Preliminary data for EhCaBP2 crystals in complex with an IQ motif are also reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 417–420. Published online 2005 April 1. doi: 10.1107/S1744309105007955
PMCID:
PMC1952423
Fang Yuan, Zhiyong Lou, Xiaofeng Li, Yu Wai Chen, John I. Bell, Zihe Rao, George F. Gao

Domain III of the West Nile virus envelope protein, the putative receptor-binding domain, was reported to be intrinsically unstable and has defied previous crystallization attempts. It has now been purified from inclusion bodies by protein refolding and was crystallized using the hanging-drop vapour-diffusion method at 291 K.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 421–423. Published online 2005 April 1. doi: 10.1107/S1744309105008195
PMCID:
PMC1952430
Mohammed O. Badasso, Dwight L. Anderson

ϕ29 bacteriophage scaffolding protein (gp7) has been overproduced in E. coli, purified, crystallized and characterized by X-ray diffraction. Two distinct crystal forms were obtained and a diffraction data set was collected to 1.8 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 424–426. Published online 2005 April 1. doi: 10.1107/S1744309105008511
PMCID:
PMC1952437
Inge Van Molle, Lieven Buts, Fanny Coppens, Liu Qiang, Lode Wyns, Remy Loris, Julie Bouckaert, Henri De Greve

The periplasmic chaperone FaeE of E. coli F4 fimbriae crystallizes in three crystal forms.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 427–431. Published online 2005 April 1. doi: 10.1107/S1744309105008432
PMCID:
PMC1952412
Noriko Higashi, Takanori Matsuura, Atsushi Nakagawa, Kazuhiko Ishikawa

l-Threonine dehydrogenase from the hyperthermophilic archaeon P. horikoshii was crystallized and preliminary X-ray crystallographic analysis was carried out.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 432–434. Published online 2005 April 1. doi: 10.1107/S174430910500881X
PMCID:
PMC1952418
F. J. López-Jaramillo, F. Pérez-Banderas, F. Hernández-Mateo, F. Santoyo-González

The feasibility of glycosylation post-purification has been demonstrated by introducing glucose into the model protein lysozyme via a novel reaction that is compatible with biological samples.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 435–438. Published online 2005 April 1. doi: 10.1107/S1744309105008869
PMCID:
PMC1952424
Yasufumi Umena, Kazuko Yorita, Takeshi Matsuoka, Makoto Abe, Akiko Kita, Kiyoshi Fukui, Tomitake Tsukihara, Yukio Morimoto

An X-ray crystallographic study of a LOX mutant in which Arg181 is replaced by Met was initiated in order to understand the functions of the conserved amino-acid residues around the FMN in the enzyme active site. LOX-R181M crystals belong to the tetragonal space group I422, with unit-cell parameters a = b = 192.632, c = 200.263 Å, α = β = γ = 90°.

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 439–441. Published online 2005 April 1. doi: 10.1107/S1744309105009152
PMCID:
PMC1952436
Jitka Vévodová, Ross M. Graham, Evelyne Raux, Martin J. Warren, Keith S. Wilson

P. aeruginosa CobE, a protein implicated in vitamin B12 biosynthesis, has been crystallized and data on the native and SeMet forms recorded to resolutions of 1.9 and 1.7 Å, respectively. The anomalous measurements will be used for phasing.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 442–444. Published online 2005 April 1. doi: 10.1107/S1744309105006731
PMCID:
PMC1952438
Karen Fulan Discola, Marcos Antonio de Oliveira, Gustavo Monteiro Silva, José Antonio Barcena, Pablo Porras, Alicia Padilla, Luis Eduardo Soares Netto, Beatriz Gomes Guimarães

Glutaredoxin 2 (Grx2) from S. cerevisiae was expressed, purified and crystallized using the hanging-drop vapour-diffusion method. Crystals were obtained from protein treated with t-butyl hydroperoxide and from a sample not submitted to pre-treatment. Both crystals belong to the tetragonal space group P41212, with very similar unit-cell parameters, and diffraction data were collected to 2.05 and 2.15 Å

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 445–447. Published online 2005 April 1. doi: 10.1107/S1744309105008730
PMCID:
PMC1952414
Luke Bailey, Sean Agger, Luke Peterson, James Thompson, Todd Weaver

A truncated form of hemolysin A from P. mirabilis capable of activating hemolytic activity has been cloned, expressed, purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 April 1; 61(Pt 4): 448–450. Published online 2005 April 1. doi: 10.1107/S1744309105009589
PMCID:
PMC1952420

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography