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Volume 61(Pt 12);  2005 December 1

Structural Genomics Communications

Makoto Nakabayashi, Naoki Shibata, Hirofumi Komori, Yasufumi Ueda, Hitoshi Iino, Akio Ebihara, Seiki Kuramitsu, Yoshiki Higuchi

The crystal structure of a conserved hypothetical protein, TTHA0849 from T. thermophilus HB8, has been determined at 2.4 Å resolution. The compact α+β structure shows the typical folding of the steroidogenic acute regulatory-related lipid-transfer (START) domain.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1027–1031. Published online 2005 November 5. doi: 10.1107/S1744309105035372
PMCID:
PMC1978151

Crystallization Communications

Alberto Cassetta, Tomaž Büdefeld, Tea Lanišnik Rižner, Katja Kristan, Jure Stojan, Doriano Lamba

The expression, purification and crystallization of 17β-hydroxysteroid dehydrogenase from the filamentous fungus C. lunatus and its Y167F mutant, both in the apo form, are described. X-ray diffraction analysis and phasing by Patterson-search techniques are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1032–1034. Published online 2005 November 5. doi: 10.1107/S1744309105034949
PMCID:
PMC1978160
Bernard Collins, Raymond C. Stevens, Rebecca Page

It is shown how protein crystallization results can be used to identify buffers that improve protein solubility and, in turn, crystallization success.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1035–1038. Published online 2005 November 5. doi: 10.1107/S1744309105035244
PMCID:
PMC1978149
Heidi Asschenfeldt Ernst, Martin Willemoës, Leila Lo Leggio, Gordon Leonard, Paul Blum, Sine Larsen

Crystallization of the α-glucosidase MalA from S. solfataricus belonging to glycoside hydrolase family 31.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1039–1042. Published online 2005 November 5. doi: 10.1107/S1744309105035177
PMCID:
PMC1978162
Shabir Najmudin, Catarina I. P. D. Guerreiro, Luís M. A. Ferreira, Maria J. C. Romão, Carlos M. G. A. Fontes, José A. M. Prates

The two C-terminal domains of the cellulase ctCel9D-Cel44A from C. thermocellum cellulosome have been crystallized in tetragonal space group P43212 and X-ray diffraction data have been collected to 2.1 and 2.8 Å from native and seleno-l-methionine-derivative crystals, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1043–1045. Published online 2005 November 5. doi: 10.1107/S1744309105035670
PMCID:
PMC1978147
Yoshitaka Nakajima, Kiyoshi Ito, Yue Xu, Nozomi Yamada, Yuko Onohara, Takashi Ito, Tadashi Yoshimoto

P. gingivalis prolyl tripeptidyl aminopeptidase has been crystallized by the vapour-diffusion method. Diffraction data have been collected and processed to 2.1 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1046–1048. Published online 2005 November 5. doi: 10.1107/S1744309105035712
PMCID:
PMC1978158
Celeste MacElrevey, Joseph E. Wedekind

Well diffracting decamer crystals of the hepatitis delta virus RNA-editing site were prepared, but exhibited merohedral twinning and base averaging owing to duplex symmetry. A longer asymmetric construct that includes additional flanking RNA sequences has been crystallized that does not appear to exhibit these defects.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1049–1053. Published online 2005 November 12. doi: 10.1107/S1744309105035888
PMCID:
PMC1978144
Christian Brüx, Karsten Niefind, Alon Ben-David, Maya Leon, Gil Shoham, Yuval Shoham, Dietmar Schomburg

The crystallization and preliminary X-ray analysis of a β-d-xylosidase from G. stearothermophilus T-6, a family 43 glycoside hydrolase, is described. Native and catalytic inactive mutants of the enzymes were crystallized in two different space groups, orthorhombic P21212 and tetragonal P41212 (or the enantiomorphic space group P43212), using a sensitive cryoprotocol. The latter crystal form

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1054–1057. Published online 2005 November 12. doi: 10.1107/S1744309105036262
PMCID:
PMC1978154
Jun Miura-Ohnuma, Tsuyoshi Nonaka, Shizue Katoh, Katsuyoshi Murata, Akiko Kita, Kunio Miki, Etsuko Katoh

Crystals of OsAGPR were obtained using the sitting-drop vapour-diffusion method at 293 K and diffract X-rays to at least 1.8 Å resolution. They belong to the hexagonal space group P61, with unit-cell parameters a = 86.11, c = 316.3 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1058–1061. Published online 2005 November 24. doi: 10.1107/S1744309105035384
PMCID:
PMC1978146
Alexandra M. Deaconescu, Seth A. Darst

Crystals of the 130 kDa E. coli Mfd protein have been grown and analysed by X-ray diffraction techniques to 3.2 Å resolution. Phases were obtained by single-wavelength anomalous dispersion from selenomethionyl-substituted crystals.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1062–1064. Published online 2005 November 24. doi: 10.1107/S1744309105035876
PMCID:
PMC1978150
Clare Peters-Libeu, Yvonne Newhouse, Preethi Krishnan, Kenneth Cheung, Elizabeth Brooks, Karl Weisgraber, Steven Finkbeiner

Optimization of crystallization conditions and cryoprotectants decreased the anisotropy of the diffraction obtained from 3B5H10 Fab crystals. Dehydration improved the resolution of cryoprotected 3B5H10 crystals from 2.6 to 1.9 Å, but changed the space group of the crystals from P21212 to P21.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1065–1068. Published online 2005 November 24. doi: 10.1107/S1744309105036547
PMCID:
PMC1978161
David M. Anstrom, Leslie Colip, Brian Moshofsky, Eric Hatcher, S. James Remington

Alanine and glutamine mutations were made to the same 15 lysine positions on the surface of E. coli malate synthase G and the impact on crystallization observed. The results support lysine replacement for improvement of crystallization and provide insight into site selection and type of amino-acid replacement.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1069–1074. Published online 2005 November 24. doi: 10.1107/S1744309105036559
PMCID:
PMC1978152
Jeyaraman Jeyakanthan, Junichiro Taka, Akihiro Kikuchi, Chizu Kuroishi, Katsuhide Yutani, Yoshitugu Shiro

The crystallization and preliminary X-ray diffraction analysis of the l-fuculose-1-phosphate aldolase (FucA) from T. thermophilus HB8. Native diffraction data set was collected to a resolution of 1.9 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1075–1077. Published online 2005 November 24. doi: 10.1107/S1744309105036766
PMCID:
PMC1978142
Wenxin Hu, Qihai Wang, Ruchang Bi

The 31.3 kDa Ap4A hydrolase from Shigella flexneri 2a has been cloned, expressed and purified using an Escherichia coli expression system. Crystals of Ap4A hydrolase have been obtained by the hanging-drop technique at 291 K using PEG 550 MME as precipitant.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1078–1080. Published online 2005 November 24. doi: 10.1107/S174430910503722X
PMCID:
PMC1978153
Zara Marland, Travis Beddoe, Leyla Zaker-Tabrizi, Ross L. Coppel, Paul K. Crellin, Jamie Rossjohn

A lipoprotein implicated in mycobacterial cell-wall biosynthesis, LpqW, was expressed in E. coli. Crystals were obtained that diffracted to 2.4 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1081–1083. Published online 2005 November 24. doi: 10.1107/S1744309105037541
PMCID:
PMC1978156
N. Umadevi, S. Kumar, N. Narayana

The first crystallographic study of an isolated WW domain is reported. Single crystals of the WW4 domain of the Nedd4-2 ubiquitin–protein ligase contain a high solvent content of 74% and diffract X-rays to 2.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1084–1086. Published online 2005 November 24. doi: 10.1107/S174430910503767X
PMCID:
PMC1978143
Dileep Vasudevan, Gayathri Gopalan, Zengyong He, Sheng Luan, Kunchithapadam Swaminathan

Crystallization of Arabidopsis thaliana cyclophilin 38. The crystal diffracts X-rays to 2.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1087–1089. Published online 2005 November 24. doi: 10.1107/S1744309105037681
PMCID:
PMC1978155
Tatyana Sandalova, Jakob Michaëlsson, Robert A. Harris, Hans-Gustaf Ljunggren, Klas Kärre, Gunter Schneider, Adnane Achour

Mouse MHC class I H-2Db in complex with human β2m and the LCMV-derived peptide gp33 has been produced and crystallized. Resolution of the structure of this complex combined with the structural comparison with the previously solved crystal structure of H-2Db/mβ2m/gp33 should lead to a better understanding of how the β2m subunit affects the overall conformation of MHC complexes

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1090–1093. Published online 2005 November 24. doi: 10.1107/S1744309105037942
PMCID:
PMC1978157
Aleksandra A. Watson, Christopher A. O’Callaghan

Recombinant human CLEC-2 was crystallized in the orthorhombic space group P212121 and X-ray diffraction data were collected to 2.0 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1094–1096. Published online 2005 November 24. doi: 10.1107/S1744309105037991
PMCID:
PMC1978148
Anna Zawacka, Bernhard Loll, Jacek Biesiadka, Wolfram Saenger, Barbara Uchanska-Ziegler, Andreas Ziegler

The crystallization of HLA-B*2706 in complex with two peptides is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1097–1099. Published online 2005 November 24. doi: 10.1107/S1744309105037966
PMCID:
PMC1978159
Hirokazu Nishida, Daisuke Tsuchiya, Yoshizumi Ishino, Kosuke Morikawa

Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 December 1; 61(Pt 12): 1100–1102. Published online 2005 November 29. doi: 10.1107/S1744309105038649
PMCID:
PMC1978145

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography