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Volume 62(Pt 8);  2006 August 1

Protein Structure Communications

Balvinder Dhaliwal, Jingshan Ren, Michael Lockyer, Ian Charles, Alastair R. Hawkins, David K. Stammers

The crystal structure of S. aureus cytidine monophosphate kinase in complex with cytidine 5′-monophosphate has been determined.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 710–715. Published online 2006 July 24. doi: 10.1107/S174430910602447X
PMCID:
PMC2242935
A. Vujičić-Žagar, B. W. Dijkstra

Two new crystal structures of A. niger α-amylase are reported, one of which reveals two hitherto unobserved maltose-binding sites.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 716–721. Published online 2006 July 24. doi: 10.1107/S1744309106024729
PMCID:
PMC2242925
Catherine Regni, Grant S. Shackelford, Lesa J. Beamer

Two structures of the enzyme phosphomannomutase/phosphoglucomutase in complex with ribose 1-phosphate and xylose 1-phosphate are described. Despite structural differences from the enzyme’s preferred phosphohexose substrates, both ligands trigger the interdomain rotation required for catalysis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 722–726. Published online 2006 July 24. doi: 10.1107/S1744309106025887
PMCID:
PMC2242917
Tomoya Kitatani, Yoshihiro Nakamura, Kei Wada, Takayoshi Kinoshita, Masahiro Tamoi, Shigeru Shigeoka, Toshiji Tada

The crystal structure of NADP-dependent apo-glyceraldehyde-3-phosphate dehydrogenase from Synechococcus PCC 7942 was determined at 2.9 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 727–730. Published online 2006 July 29. doi: 10.1107/S1744309106027916
PMCID:
PMC2242934

Structural Genomics Communications

J. Rajan Prabu, S. Thamotharan, Jasbeer Singh Khanduja, Emily Zabala Alipio, Chang-Yub Kim, Geoffrey S. Waldo, Thomas C. Terwilliger, Brent Segelke, Tim Lekin, Dominique Toppani, Li-Wei Hung, Minmin Yu, Evan Bursey, K. Muniyappa, Nagasuma R. Chandra, M. Vijayan

RuvA, a protein from M. tuberculosis H37Rv involved in recombination, has been cloned, expressed, purified and analysed by X-ray crystallography.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 731–734. Published online 2006 July 24. doi: 10.1107/S1744309106024791
PMCID:
PMC2242936
Bichitra K. Biswal, Karolyn Au, Maia M. Cherney, Craig Garen, Michael N. G. James

The crystal structure of a probable pyridoxine 5′-phosphate oxidase, Rv2074 from M. tuberculosis, has been solved by the two-wavelength anomalous dispersion method and has been refined at 1.6 Å resolution. Two citric acid molecules are bound fortuitously to the possible active site of Rv2074.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 735–742. Published online 2006 July 24. doi: 10.1107/S1744309106025012
PMCID:
PMC2242915

Crystallization Communications

Yi-Hung Lin, Wen-Yan Peng, Yen-Chieh Huang, Hong-Hsiang Guan, Ying-Cheng Hsieh, Ming-Yih Liu, Tschining Chang, Chun-Jung Chen

The crystallization of rice α-amylase/subtilisin bifunctional inhibitor is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 743–745. Published online 2006 July 24. doi: 10.1107/S1744309106023335
PMCID:
PMC2242909
Bettina Bäuerle, Tatyana Sandalova, Gunter Schneider, Paul-Gerhard Rieger

This is the first report of the crystallization of an IDS-epimerase from A. tumefaciens BY6 and its l-selenomethionine derivative.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 746–749. Published online 2006 July 24. doi: 10.1107/S1744309106024195
PMCID:
PMC2242926
S. R. Marana, F. C. Cançado, A. A. Valério, C. Ferreira, W. R. Terra, J. A. R. G. Barbosa

The digestive lysozymes 1 and 2 from M. domestica were crystallized by vapour diffusion. The crystallographic data were processed to a maximum resolution of 1.9 Å in both cases.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 750–752. Published online 2006 July 24. doi: 10.1107/S1744309106024201
PMCID:
PMC2242927
Robert Janowski, Ali Nasser Eddine, Stefan H. E. Kaufmann, Manfred S. Weiss

M. tuberculosis hypothetical protein Rv2827c was cloned, expressed, purified and crystallized. Preliminary X-ray diffraction data were collected to a resolution of 1.93 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 753–756. Published online 2006 July 24. doi: 10.1107/S1744309106024213
PMCID:
PMC2242928
Josiah Obiero, Sara A. Bonderoff, Meghan M. Goertzen, David A. R. Sanders

Recombinant D. radiodurans TrxR with a His tag at the N-terminus was expressed in Escherichia coli and purified by metal-affinity chromatography. The protein was crystallized using the sitting-drop vapour-diffusion method in the presence of 35% PEG 4000, 0.2 M ammonium acetate and citric acid buffer pH 5.1 at 293 K.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 757–760. Published online 2006 July 24. doi: 10.1107/S1744309106024845
PMCID:
PMC2242918
M. J. Knight, A. Ruaux, H. Mikolajek, P. T. Erskine, R. Gill, S. P. Wood, M. Wood, J. B. Cooper

BipD is likely to be a component of a type-III protein secretion system (TTSS) in B. pseudomallei. Native and selenomethionyl-BipD proteins have been expressed and crystals have been obtained which diffract to 2.1 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 761–764. Published online 2006 July 24. doi: 10.1107/S1744309106024857
PMCID:
PMC2242920
L. C. Corrêa, D. P. Marchi-Salvador, A. C. O. Cintra, A. M. Soares, M. R. M. Fontes

A myotoxic Asp49-PLA2 with low catalytic activity from B. jararacussu (BthTX-II) was crystallized in the monoclinic crystal system; a complete X-ray diffraction data set was collected and a molecular-replacement solution was obtained. The oligomeric structure of BthTX-II resembles those of the Asp49-PLA2 PrTX-III and all bothropic Lys49-PLA2s.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 765–767. Published online 2006 July 24. doi: 10.1107/S1744309106025164
PMCID:
PMC2242931
Eloise Mastrangelo, Michela Bollati, Mario Milani, Xavier de Lamballeire, Nadege Brisbare, Karen Dalle, Violaine Lantez, Marie-Pierre Egloff, Bruno Coutard, Bruno Canard, Ernest Gould, Naomi Forrester, Martino Bolognesi

Two methyltransferases from flaviviruses (Meaban and Yokose viruses) have been overexpressed and crystallized. Diffraction data and characterization of the two crystal forms are presented, together with a preliminary molecular-replacement solution for both enzymes.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 768–770. Published online 2006 July 24. doi: 10.1107/S1744309106025553
PMCID:
PMC2242907
Shunfu Piao, Ji Young Jung, Ji Won Park, Jaewon Lee, Bok Leul Lee, Nam-Chul Ha

Prophenoloxidase (proPO) activating factor-I (PPAF-I) is a catalytically active clip-domain SP, cleaves which proPO. The results of crystallization and preliminary X-ray analysis of the SP domain of PPAF-I are reported here.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 771–773. Published online 2006 July 24. doi: 10.1107/S1744309106025851
PMCID:
PMC2242929
Martin Jinek, Elena Conti

The catalytic domain of the murine glycosyltransferase Manic Fringe was expressed in insect cells. Removal by site-directed mutagenesis of two N-glycosylation sites present in the protein was essential to obtain crystals that diffracted to 1.8 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 774–777. Published online 2006 July 24. doi: 10.1107/S174430910602611X
PMCID:
PMC2242911
Ling Xu, Svetlana E. Sedelnikova, Patrick J. Baker, David W. Rice

SA0961 is an unknown hypothetical protein from Staphylococcus aureus that can be identified in the Firmicutes division of Gram-positive bacteria. SA0961 was cloned and the protein was overexpressed in Escherichia coli, purified and subsequently crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 778–780. Published online 2006 July 24. doi: 10.1107/S1744309106027400
PMCID:
PMC2242921
Dong-Ju You, Hyongi Chon, Yuichi Koga, Kazufumi Takano, Shigenori Kanaya

Type 1 RNase H from the hyperthermophilic archaeon S. tokodaii 7 was overproduced in E. coli, purified, and crystallized. Preliminary crystallographic studies indicated that the crystal belongs to space group P43, with unit-cell parameters a = b = 39.21, c = 91.15 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 781–784. Published online 2006 July 25. doi: 10.1107/S1744309106024420
PMCID:
PMC2242919
Tsuyoshi Imasaki, Toshiyuki Shimizu, Hiroshi Hashimoto, Yuji Hidaka, Michiyuki Yamada, Mamoru Sato

Transportin 1 was cocrystallized with nucleocytoplasmic shuttling fragments of JKTBP and hnRNP D and a nuclear localization fragment of TAP. X-ray diffraction data were collected using synchrotron radiation at SPring-8.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 785–787. Published online 2006 July 25. doi: 10.1107/S1744309106025711
PMCID:
PMC2242924
Neratur K. Lokanath, Naoki Kunishima

The archaeal phosphoglycerate mutase PH0037 from P. horikoshii OT3 has been crystallized in space group R32, with unit-cell parameters a = 155.62, c = 230.35 Å. A 2.2 Å resolution data was collected at SPring-8 beamline BL26B1.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 788–790. Published online 2006 July 25. doi: 10.1107/S1744309106026121
PMCID:
PMC2242930
Shouhei Mine, Tsutomu Nakamura, Kunio Hirata, Kazuhiko Ishikawa, Yoshihisa Hagihara, Koichi Uegaki

The expression, purification and preliminary X-ray diffraction analysis of a catalytic domain of a chitinase from P. furiosus is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 791–793. Published online 2006 July 25. doi: 10.1107/S1744309106026157
PMCID:
PMC2242910
Simone Weyand, Georgia Kefala, Manfred S. Weiss

M. tuberculosis N-succinyldiaminopimelate aminotransferase, the enzyme which catalyzes the sixth reaction in the lysine-biosynthesis pathway, has been cloned, expressed, purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 794–797. Published online 2006 July 25. doi: 10.1107/S1744309106026753
PMCID:
PMC2242922
Watchalee Chuenchor, Salila Pengthaisong, Jirundon Yuvaniyama, Rodjana Opassiri, Jisnuson Svasti, James R. Ketudat Cairns

Rice BGlu1 β-glucosidase was purified from recombinant E. coli and crystallized with and without the inhibitor 2-deoxy-2-fluoro-β-d-glucose. The crystals diffracted to 2.15 and 2.75 Å, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 798–801. Published online 2006 July 25. doi: 10.1107/S1744309106027084
PMCID:
PMC2242908
Yang Liu, J. Sivaraman, Choy L. Hew

The nonstructural protein VP9 from white spot syndrome virus (WSSV) has been identified and expressed in Escherichia coli. Native protein was purified and crystallized by vapour diffusion.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 802–804. Published online 2006 July 25. doi: 10.1107/S1744309106027436
PMCID:
PMC2242916
José Trincão, Marta Sousa Silva, Lídia Barata, Cecília Bonifácio, Sandra Carvalho, Ana Maria Tomás, António E. N. Ferreira, Carlos Cordeiro, Ana Ponces Freire, Maria João Romão

A glyoxalase II from L. infantum was cloned, purified and crystallized and its structure was solved by X-ray crystallography.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 805–807. Published online 2006 July 25. doi: 10.1107/S1744309106027539
PMCID:
PMC2242913
Dean Rea, Carole Hazell, Norma W. Andrews, Rory E. Morty, Vilmos Fülöp

Recombinant oligopeptidase B from T. brucei has been prepared and crystallized. Data were collected to 2.7 Å. Heavy-atom soaks and preparation of selenomethionine-substituted protein are in progress for structure determination by MAD or MIR.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 808–810. Published online 2006 July 25. doi: 10.1107/S1744309106027874
PMCID:
PMC2242912
Peter M. Brown, Tom T. Caradoc-Davies, James M. Dickson, Garth J. S. Cooper, Kerry M. Loomes, Edward N. Baker

Mouse myo-inositol oxygenase, a key enzyme involved in inositol catabolism, has been expressed, purified and crystallized in a form suitable for structure determination by X-ray crystallography.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 811–813. Published online 2006 July 25. doi: 10.1107/S1744309106028144
PMCID:
PMC2242914
Minglian Zhao, Zhenguo Li, Wei Zheng, Zhiyong Lou, Guo-Qiang Chen

The phasin PhaPAh from A. hydrophila strain 4AK4 was crystallized using the hanging-drop vapour-diffusion method.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 814–819. Published online 2006 July 26. doi: 10.1107/S1744309106025000
PMCID:
PMC2242923
Ivana Tomčová, Rui Miguel Mamede Branca, Gabriella Bodó, Csaba Bagyinka, Ivana Kutá Smatanová

Cross crystallization is reported as a new crystallization procedure used for growing protein crystals and preliminary diffraction analysis of a newly discovered protein, cytochrome c 4, is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 820–824. Published online 2006 July 26. doi: 10.1107/S1744309106027710
PMCID:
PMC2242932
My D. Sam, Mohamad A. Abbani, Duilio Cascio, Reid C. Johnson, Robert T. Clubb

This paper describes the crystallization, dehydration and preliminary X-ray data analysis of a complex containing several bacteriophage lambda excisionase (Xis) proteins cooperatively bound to a 33-mer DNA duplex (Xis–DNAX1-X2).

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 August 1; 62(Pt 8): 825–828. Published online 2006 July 26. doi: 10.1107/S1744309106027643
PMCID:
PMC2242933

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography