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Volume 68(Pt 8);  2012 August 1

Structural Communications

Michele Ferrari, Claudia Folli, Elisa Pincolini, Timothy S. McClintock, Manfred Rössle, Rodolfo Berni, Michele Cianci

The two recombinant apo subunits H1 and H2 from H. americanus have been structurally characterized. Reconstitution studies with astaxanthin reproduced the bathochromic shift of 85–95 nm typical of the natural crustacyanin subunits.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 846–853. Published online 2012 July 26. doi: 10.1107/S1744309112026103
PMCID:
PMC3412759
Udesh de Silva, Zhaoli Zhou, Bernard A. Brown, II

The crystal structure of A. pernix fibrillarin includes a tightly bound S-adenosyl-l-methionine molecule.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 854–859. Published online 2012 July 26. doi: 10.1107/S1744309112026528
PMCID:
PMC3412760
Lorien J. Parker, Hisami Watanabe, Keiko Tsuganezawa, Yuri Tomabechi, Noriko Handa, Mikako Shirouzu, Hitomi Yuki, Teruki Honma, Naoko Ogawa, Tetsuo Nagano, Shigeyuki Yokoyama, Akiko Tanaka

The structures of Pim1 kinase in complex with in silico screening hits and several subsequently optimized inhibitors, are reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 860–866. Published online 2012 July 31. doi: 10.1107/S1744309112027108
PMCID:
PMC3412761
David Ruiz Carrillo, Ramya Chandrasekaran, Martina Nilsson, Tobias Cornvik, Chong Wai Liew, Suet Mien Tan, Julien Lescar

The crystal structure of human Rack1 protein is reported at 2.45 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 867–872. Published online 2012 July 26. doi: 10.1107/S1744309112027480
PMCID:
PMC3412762
Alexander Pflug, Kenneth A. Johnson, Richard A. Engh

The anomalous dispersion signal of the bromine-containing kinase inhibitor H-89 was used to characterize discrete binding modes of the compound when complexed with the catalytic subunit of protein kinase A.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 873–877. Published online 2012 July 26. doi: 10.1107/S1744309112028655
PMCID:
PMC3412763
David von Stetten, Marjolaine Noirclerc-Savoye, Joachim Goedhart, Theodorus W. J. Gadella, Jr, Antoine Royant

The first structure of a fluorescent protein derived from A. victoria green fluorescent protein bearing the obligate-monomer mutation A206K is presented and shows no significant structural change in the vicinity of the fluorescent chromophore.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 878–882. Published online 2012 July 26. doi: 10.1107/S1744309112028667
PMCID:
PMC3412764
Rafał Dolot, Magdalena Ozga, Artur Włodarczyk, Agnieszka Krakowiak, Barbara Nawrot

The atomic structure of a new crystal form of the human histidine triad nucleotide-binding protein 1 (hHINT1)–AMP complex is reported at 1.38 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 883–888. Published online 2012 July 27. doi: 10.1107/S1744309112029491
PMCID:
PMC3412765
Edward L. D’Antonio, David W. Christianson

Crystal structures of Mn2 2+- and Co2 2+-substituted human arginase I complexed with an unreactive analog of substrate l-arginine are described at 1.82 and 1.90 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 889–893. Published online 2012 July 27. doi: 10.1107/S1744309112027820
PMCID:
PMC3412766

Crystallization Communications

Fan Zhang, Li Xing, Maikun Teng, Xu Li

The ribosome-dependent mRNA interferase YafO from Escherichia coli belongs to a type II toxin–antitoxin (TA) system and its cognate antitoxin YafN neutralizes cell toxicity by forming a stable YafN–YafO complex. Here, the YafN–YafO complex has been expressed and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 894–897. Published online 2012 July 31. doi: 10.1107/S1744309112024037
PMCID:
PMC3412767
Shaowei Liu, Tingting Ran, Xiang Shen, Langlai Xu, Weiwu Wang, Dongqing Xu

The last step in the biosynthetic pathway of 4-methoxy-2,2′-bipyrrole-5-­carbaldehyde (MBC) is catalyzed by PigF, which transfers a methyl group to 4-­hydroxy-2,2′-bipyrrole-5-carbaldehyde (HBC) to form the terminal product MBC, but its catalytic mechanism is not known. To elucidate its mechanism, recombinant PigF was purified and crystallized.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 898–901. Published online 2012 July 31. doi: 10.1107/S1744309112024001
PMCID:
PMC3412768
Zhao Liu, Zhongzhou Chen, Wei Wu

Crystals of apo and SeMet ferric uptake regulator from the magnetotactic bacterium M. gryphiswaldense MSR-1 were obtained and optimized to obtain high-quality diffraction data. Diffraction data sets were collected and processed at 1.58 and 1.9 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 902–905. Published online 2012 July 31. doi: 10.1107/S1744309112024311
PMCID:
PMC3412769
Yi-Che Su, Zhi-Le Tu, Chao-Yu Yang, Ko-Hsin Chin, Mary Lay-Cheng Chuah, Zhao-Xun Liang, Shan-Ho Chou

Crystals of native and selenomethionine-labelled STING138–344 protein from M. musculus diffracted to resolutions of 2.39 and 2.2 Å, respectively

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 906–910. Published online 2012 July 31. doi: 10.1107/S1744309112024372
PMCID:
PMC3412770
Yoko Chiba, Shoichiro Horita, Jun Ohtsuka, Hiroyuki Arai, Koji Nagata, Yasuo Igarashi, Masaru Tanokura, Masaharu Ishii

A novel type of phosphoserine phosphatase from H. thermophilus TK-6 was heterologously expressed in E. coli, purified and crystallized by the sitting-drop vapour-diffusion method. The crystals obtained belonged to space group P212121 and diffracted X-rays to 1.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 911–913. Published online 2012 July 31. doi: 10.1107/S1744309112025213
PMCID:
PMC3412771
Wenting Liu, Yu Sun, Tzu-Ping Ko, Juergen Wiegel, Weilan Shao, Fuping Lu, Rey-Ting Guo, Chun-Hsiang Huang

Xylosidase C from Thermoanaerobacterium saccharolyticum, the first enzyme classified as GH120 family, has been successfully crystallized. The crystal diffracted to a resolution of 2.2 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 914–916. Published online 2012 July 31. doi: 10.1107/S1744309112025328
PMCID:
PMC3412772
Abd Ghani Abd Aziz, Svetlana E. Sedelnikova, Sergey N. Ruzheinikov, Simon Thorpe, Rahmah Mohamed, Sheila Nathan, John B. Rafferty, Patrick J. Baker, David W. Rice

The receiver domain of a putative response regulator from B. pseudomallei, BPSL0128, has been crystallized in a form suitable for X-ray analysis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 917–922. Published online 2012 July 31. doi: 10.1107/S1744309112025791
PMCID:
PMC3412773
Francesco Fersini, Yuliya Dall’Antonia, Jennifer Chow, Wolfgang R. Streit, Jochen Mueller-Dieckmann

A putative lipase, LipS, from the soil metagenome of an environmental sample taken at the Biocenter Klein Flottbek, Hamburg, Germany was cloned, expressed and purified. Two different constructs of the protein were crystallized in a search for well diffracting crystals. Preliminary X-ray analyses indicated that crystals in space groups P42212 and P4 diffracted X-ray radiation to 2.8 and 2.0 Å

...
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 923–926. Published online 2012 July 31. doi: 10.1107/S1744309112025651
PMCID:
PMC3412774
Natalie Zeytuni, Tal Offer, Geula Davidov, Raz Zarivach

The C-terminal domain of the cation diffusion facilitator MamM has been successfully constructed, purified and crystallized. Diffraction data was collected to to a resolution of 2.0 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 927–930. Published online 2012 July 31. doi: 10.1107/S1744309112025638
PMCID:
PMC3412775
Anurag Sharma, Gottfried J. Palm, Moni Kumari, Santosh Panjikar, M. V. Jagannadham, Winfried Hinrichs

Crystals of a new peroxidase, named banyan peroxidase, from the latex of F. benghalensis belonged to the trigonal space group P3221 and provided X-ray diffraction data to a resolution of 1.66 Å. The anomalous signal of the intrinsic iron and calcium ions was sufficent for phasing by SAD.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 931–934. Published online 2012 July 31. doi: 10.1107/S1744309112026097
PMCID:
PMC3412776
Carlos A. H. Fernandes, Elaine C. G. Gartuzo, Ivan Pagotto, Edson J. Comparetti, Salomón Huancahuire-Vega, Luis Alberto Ponce-Soto, Tássia R. Costa, Sergio Marangoni, Andreimar M. Soares, Marcos R. M. Fontes

Two myotoxic and noncatalytic Lys49-phospholipases A2 (braziliantoxin-II and MT-II) and a myotoxic and catalytic phospholipase A2 (braziliantoxin-III) from B. brazili were crystallized. X-ray diffraction data sets were collected and molecular-replacement solutions were obtained.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 935–938. Published online 2012 July 31. doi: 10.1107/S1744309112026073
PMCID:
PMC3412777
Mads Gabrielsen, Alan Riboldi-Tunnicliffe, Marina Ibáñez-Shimabukuro, Kate Griffiths, Andrew J. Roe, Alan Cooper, Brian O. Smith, Betina Córsico, Malcolm W. Kennedy

As-p18, an unusual fatty-acid-binding protein from a parasitic nematode, was expressed in bacteria, purified and crystallized. The use of a microfocus beamline was essential for data collection.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 939–941. Published online 2012 July 31. doi: 10.1107/S1744309112026553
PMCID:
PMC3412778
Xiang Xu, Xiaoai Zhang, Guangwen Lu, Yongping Cai

Nectin-4 belongs to a family of immunoglobulin-like cell adhesion molecules and is highly expressed in cancer cells. Recently, nectin-4 was found to be a receptor of measles virus and the IgV domain sustains strong binding to measles virus H protein. In this study, the successful expression and purification of human nectin-4 V domain (nectin-4v) is reported

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 942–945. Published online 2012 July 31. doi: 10.1107/S1744309112027236
PMCID:
PMC3412779
Ming Dong Yao, Ken-ichi Miyazono, Jun Ohtsuka, Setsu Hirano, Koji Nagata, Sueharu Horinouchi, Yasuo Ohnishi, Masaru Tanokura

The DNA-binding domain of AdpA, the central transcription factor in the A-factor regulatory cascade in the filamentous bacterium S. griseus, was heterologously expressed, purified and crystallized in complex with a duplex DNA by the sitting-drop vapour-diffusion method. The best crystal diffracted X-rays to 2.8 Å resolution and belonged to space group C2221.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 946–949. Published online 2012 July 31. doi: 10.1107/S1744309112026899
PMCID:
PMC3412780
Jichun Ma, Wai Kwan Tang, Lothar Esser, Ira Pastan, Di Xia

The therapeutic antibody MORAb-009 disrupts the interaction of mesothelin and the ovarian cancer antigen CA-125. Crystals have been grown of the Fab fragment derived from MORAb-009 and of its complex with an N-terminal fragment of mesothelin.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 950–953. Published online 2012 July 31. doi: 10.1107/S1744309112028229
PMCID:
PMC3412781
Nishant Kumar Pandey, Ravi Kant Pal, Maruthi Kashyap, Neel Sarovar Bhavesh

The Escherichia coli PapD-like protein (EcpD), from uropathogenic Escherichia coli (UPEC), which is a periplasmic chaperon of Yad fimbriae was cloned, overexpressed, purified and crystallized. The crystals obtained diffracted X-rays to 1.67 Å resolution and belonged to space group C2221.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 954–957. Published online 2012 July 31. doi: 10.1107/S1744309112027364
PMCID:
PMC3412782
Linjun Guo, Masahiko Okai, Tomoko Mase, Fabiana Lica Imai, Takuya Miyakawa, Koji Nagata, Hiroyuki Yamanaka, Hidemi Fujii, Makoto Hibi, Jun Ogawa, Masaru Tanokura

asHPAL, a member of the HpaI/HpcH subfamily of class II aldolases, was expressed, purified and crystallized in the absence and presence of 2-ketobutyrate as one of its substrates using the sitting-drop vapour-diffusion method. asHPAL crystals grown without and with 2-ketobutyrate diffracted to 1.60 and 1.55 Å resolution, respectively.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 958–961. Published online 2012 July 31. doi: 10.1107/S1744309112028278
PMCID:
PMC3412783
Sotaro Kikuchi, Kodai Hara, Toshiyuki Shimizu, Mamoru Sato, Hiroshi Hashimoto

Crystallization of human REV1–REV7–REV3 ternary complex involved in translesion DNA synthesis.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 962–964. Published online 2012 July 27. doi: 10.1107/S1744309112032435
PMCID:
PMC3412784
I. Nemčovičová, M. Nemčovič, S. Šesták, M. Plšková, I. B. H. Wilson, J. Mucha

The purification and crystallization of the D. melanogaster lysosomal α-mannosidase is reported. The plate-like crystals belonged to the orthorhombic space group P212121 and diffracted to 3.5 Å resolution.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 965–970. Published online 2012 July 31. doi: 10.1107/S1744309112029375
PMCID:
PMC3412785
Daniel R. Tromans, Clare E. M. Stevenson, Rebecca J. M. Goss, David M. Lawson

Crystals of Pac17, an enzyme from the pacidamycin-biosynthetic cluster of S. coeruleorubidus, were obtained and native X-ray data were recorded to a maximum resolution of 1.9 Å.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 971–974. Published online 2012 July 31. doi: 10.1107/S1744309112029144
PMCID:
PMC3412786
Young Woo Park, Hyun Ku Yeo, Jae Young Lee

Trigonal crystals of FadR from B. halodurans have been obtained. X-ray data were collected to 2.05 Å resolution using synchrotron radiation.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 975–977. Published online 2012 July 31. doi: 10.1107/S1744309112029508
PMCID:
PMC3412787
Qiao Liu, Hong Wang, Huihui Liu, Maikun Teng, Xu Li

The crystallization and preliminary X-ray diffraction analysis of glyceraldehyde-3-phosphate dehydrogenase 3 from S. cerevisiae is reported.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 978–980. Published online 2012 July 31. doi: 10.1107/S1744309112028989
PMCID:
PMC3412788

Laboratory Communications

Sandra Puehringer, Michael Hellmig, Sunbin Liu, Manfred S. Weiss, Markus C. Wahl, Uwe Mueller

A successful MAD experiment has been conducted at the Pr L III edge on HZB beamline BL14.2.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 981–984. Published online 2012 July 31. doi: 10.1107/S1744309112025456
PMCID:
PMC3412789
Edward E. Pryor, Jr, Daniel J. Wozniak, Thomas Hollis

Crystallization of the complex of the transcription factor AmrZ with DNA was accomplished through the combination of established and newly developed methods. Here, a general method to obtain and optimize crystals of protein–DNA complexes consisting of these combined procedures is described.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 985–993. Published online 2012 July 31. doi: 10.1107/S1744309112025316
PMCID:
PMC3412790
Arne Meyer, Karsten Dierks, Dierk Hilterhaus, Thomas Klupsch, Peter Mühlig, Jens Kleesiek, Robert Schöpflin, Howard Einspahr, Rolf Hilgenfeld, Christian Betzel

A device has been developed to optimize crystal-growth conditions by experiments in a single drop.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 August 1; 68(Pt 8): 994–998. Published online 2012 July 31. doi: 10.1107/S1744309112024074
PMCID:
PMC3412791

Articles from Acta Crystallographica Section F: Structural Biology and Crystallization Communications are provided here courtesy of International Union of Crystallography