|Home | About | Journals | Submit | Contact Us | Français|
Vibriosis disease by Vibrio spp. greatly reduced productivity of aquaculture, such as brown-marbled grouper (Epinephelus fuscoguttatus), which is an economically important fish species in Malaysia. Preventive measures and immediate treatment are critical to reduce the mortality of E. fuscoguttatus from vibriosis. To investigate the molecular mechanisms associated with immune response and host-bacteria interaction, a transcriptomic analysis was performed to compare between healthy and Vibrio-infected groupers. This permits the discovery of immune-related genes, specifically the resistance genes upon infection. Herein, we provide the raw transcriptome data from Illumina HiSeq. 4000 that have been deposited into NCBI SRA database with the BioProject accession number PRJNA396437. A total of 493,403,076 raw sequences of 74.5 Gb were obtained. Trimming of the raw data produced 437,186,232 clean reads of ~58 Gb. These datasets will be useful to elucidate the defence mechanisms of E. fuscoguttatus against Vibrio vulnificus infection for future development of effective prevention and treatment of vibriosis.
Value of the data
This article reports the transcriptome data from gill and whole-body tissues of control (healthy) and V. vulnificus-infected E. fuscoguttatus at day 31 post infection. The raw data were deposited in the NCBI SRA database as detailed in Table 1.
Brown-marbled grouper (Epinephelus fuscoguttatus) fingerlings were obtained from Fisheries Research Institute (FRI) Tanjung Demong, Besut in Terengganu, Malaysia (5°46'09.0"N 102°33'02.6"E). Fingerlings with length of ~5 cm were acclimatised in aerated seawater (25 °C, salinity ~35 parts per thousand (PPT), pH 7.9) for one week. For experimental infection, healthy fingerlings were randomly selected and immersed in 20 L seawater containing 1×107 CFU mL−1 of Vibrio vulnificus for 30 min, whereas the controls were immersed in clean seawater. Both control and infected fingerlings were then transferred independently into new aquarium containing fresh seawater, and observed daily for 30 days. At day 31-post-infection, fingerlings that survived from the experimental infection and the controls were collected and flash frozen in liquid nitrogen respectively. Samples were then kept at −80 °C prior to RNA extraction.
Total RNA was extracted from gills and whole-body tissues using modified hexadecyltrimethylammonium bromide (CTAB) method . Ten biological replicates from each tissue were used for the RNA extraction. The quality and integrity of the isolated RNA were quantified using NanoDrop spectrophotometer (Thermo Fisher Scientific Inc., United States) and Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., United States), respectively. Only three replicates from each tissue with the highest RIN score were chosen for RNA sequencing. The cDNA poly (A)-containing mRNA libraries were then prepared according to the SureSelect Strand-Specific RNA Library Prep for Illumina Multiplexed Sequencing (mRNA Library Preparation Protocol) Version E0, March 2017. Libraries were then sequenced using Illumina HiSeq. 4000 platform at Theragene Etex Bio Institute (Gyeonggi-do, Republic of Korea).
Raw reads obtained were filtered by trimming the adapter sequences using cutadapt v1.14 program  with the Phred quality score of 30 (nucleotide accuracy: 99.9%) (Table 2). Clean reads were then de novo assembled using Trinity v2.4.0 .
This work was supported by Research University Grants from Universiti Kebangsaan Malaysia (UKM-GUP-2014-083 and DIP-2015-024) awarded to Syarul Nataqain Baharum. We acknowledged Azhani Abdul-Rahman for the technical support.
Transparency documentSupplementary data associated with this article can be found in the online version at doi:10.1016/j.dib.2017.11.024.