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Ethiop J Health Sci. 2017 May; 27(3): 215–226.
PMCID: PMC5614992

Correlation between Serum Lipid Profile with Anthropometric and Clinical Variables in Patients with Type 2 Diabetes Mellitus

Abstract

Background

The problem of dyslipidemia is high in patients with diabetes mellitus. There is ample evidence that abnormalities in lipid metabolism are important risk factors for increased incidence of diabetes associated complications. The most important risk indicators for these complications are lipid profile abnormalities. Therefore, the aim of this study was to assess the correlation between serum lipid profile with anthropometric and clinical variables among type 2 diabetes mellitus patients.

Methods

A comparative cross sectional study was conducted at University of Gondar Hospital from February to April in 2015. A total of 296 participants (148 case and 148 healthy controls) were selected using systematic random sampling technique. Socio-demographic characteristics and clinical data were collected using pretested structured questionnaire incorporating the WHO Stepwise approach. Fasting venous blood sample was collected for blood sugar; lipid profile investigations and the blood levels were determined by Bio Systems A25 Chemistry Analyzer (Costa Brava, Spain). Independent sample t-test and Man Whitney U test were used to compare means. P-value < 0.05 was considered statistically significant.

Results

Overall, T2DM patients had significantly higher total cholesterol ([205.4±50.9vs184.9±44.1]mg/dl), low density lipoprotein ([113.1±43.2vs100.1±36.4] mg/dl) and triacylglycerol ([189.22± 100.9 vs 115.13±59.2] mg/dl), and significant decline of high density lipoprotein cholesterol ([56.5±20.4vs62.1±13] mg/dl) as compared to healthy controls, respectively. Triacylglycerolemia was significantly associated with the risk of cardiovascular disease (AOR: 1.015; 95%CI: 1.010–1.021). Evident correlation was observed between anthropometric and clinical variables with lipid profile.

Conclusion

Higher serum levels of fasting blood sugar, total cholesterol, low density lipoprotein cholesterol, and triacylglycerol and lower levels of high density lipoprotein cholesterol are found in type 2 diabetes mellitus patients. Thus, DM patients are more prone to dyslipidemia which is an important risk factor for atherosclerosis and coronary heart disease.

Keywords: Type 2 diabetes mellitus, lipid profile, Ethiopia

Introduction

Diabetes mellitus (DM) is a group of metabolic disorder of multiple etiologies. It is characterized by chronic hyperglycemia with disturbances of carbohydrate, protein and lipid metabolism resulting from defects in insulin secretion, action, or both (1).

Diabetes Mellitus can be grouped into different clinical classes. 5–10% of diabetes cases is type 1 DM. It is mostly diagnosed during childhood ages and caused by β-cell damage resulting in diminished ability of the pancreas to produce insulin. The second class is type 2 diabetes mellitus (T2DM). It constitutes over 80–90% of all DM cases and is mostly diagnosed after the age of 40. However, younger cases are being reported recently. Insulin is usually produced but not properly utilized due to insulin resistance (IR) in the target cells and gestational DM which is defined as first recognition of DM during pregnancy (2,3).

The major chronic complications of DM are microvascular and macrovascular (4). The overall temporal burden of hyperglycemia is responsible for the complications and adverse outcomes (5). These complications are common in sub-Saharan Africa due to various reasons like lack of community awareness, poor laboratory and clinical diagnosis. As a result of the above reasons, people with DM regularly visit the health facilities because of complication rather than for routine medical checkup (6). It requires continuing medical care and ongoing patient self-management education and support to prevent and reduce complications (7). Multiple risk factors are responsible for the development of T2DM such as behavioral and environmental risk factors (8). Concordance among monozygotic twins is close to 100%, and 25% of those with the disease have a family history of DM (9). The environmental factors in the pathogenesis of T2DM are obesity, physical inactivity, increase in alcohol intake, over eating and smoking (10).

Diabetes Mellitus currently affects about 5% of the world's population, and its prevalence is rapidly increasing particularly in elderly people (11,12). It is evolving as one of the most important chronic health problems in Ethiopia (13). The estimated prevalence of DM in adult population of Ethiopia is 1.9% (13). WHO estimated the number of diabetic cases in Ethiopia as 800,000 by the year 2000, and the number is expected to increase to 1.8 million by 2030 (15). A community based study in Gondar, Northwest Ethiopia, showed that the prevalence of DM among adults aged 35 years and above was 5.1% for urban and 2.1% for rural dwellers (16).

Lipid abnormalities are prevalent in DM patients because of IR which affects key enzymes and pathways in lipid metabolism: Apo protein production, regulation of lipoprotein lipase, action of cholesterol ester transfer proteins and hepatic and peripheral actions of insulin (17). Hyperglycemia and the high level of IR associated with T2DM has multiple effects on fat metabolism which results in the production of atherogenic dyslipidemia characterized by lipoprotein abnormalities: elevated very low density lipoprotein cholesterol (VLDL) elevated low density lipoprotein cholesterol (LDL-c), elevated triacylglycerol (TAG) and decreased high density lipoprotein cholesterol (HDL-c) which are measured for cardiovascular risk prediction (1822).

The duration of diabetes, degree of hyperglycemia, hypertension, dyslipidemia and smoking are the strongest risk factors for chronic complications of DM that leads to biochemical aberrations (23). Appropriate management targeting glycemic, hypertension and lipid control is important for decreasing morbidity and mortality, and improving long term quality of life for patients diagnosed with T2DM. Lifestyle changes such as nutrition therapy, weight loss, regular physical exercise, appropriate education and self-management strategies are vital to improve outcomes (24, 25). Therefore, the aim of this study was to assess the correlation between lipid profile with anthropometric and clinical variables in patients with T2DM at University of Gondar Hospital, Northwest Ethiopia.

Methods and Materials

Study design, period and area: A comparative cross-sectional study was conducted from February to April, 2015 at Gondar University Hospital Chronic Illness Clinic, Northwest Ethiopia. Gondar Town is 738 km far from Addis Ababa, the capital city of Ethiopia. The town has around nine government health centers and one university hospital. University of Gondar Hospital provides health service for more than 5 million inhabitants in northwest Ethiopia. It plays an important role in teaching, research and community service. The hospital gives service for more than 8000 diabetic patients.

Sample size and study subjects: The sample size was calculated based on two population mean formula using Open Epi Info version 2, open source calculator by considering 95% confidence interval (2-sided) and 80% power. Ratio of sample size (T2DM group/control group) is 1. We used mean ± SD of 4.41 ± 1.39 and 3.98 ± 1.24 for cases and controls, respectively (26). Therefore, the total sample size was 296 (equal number of cases and controls). All T2DM patients attended to the institution were included using systematic random sampling technique. All T2DM patients attending at University of Gondar Hospital were included in the study. In addition, age and sex matched apparently healthy individuals were employed as a control group. On the other hand, patients who already had any chronic diseases, pregnant women and patients on statins for abnormal lipid treatment and on insulin therapy, smokers, alcoholics and those who were on anticoagulant drugs were excluded from the study.

Data collection and laboratory methods: Patients were asked to fill the written consent form after pre-test was conducted in 5% of the study participants. Data on socio-demographic characteristics were collected by trained nurses from University of Gondar Hospital Chronic Illness Clinic by using semi-structured questionnaire. Anthropometric variables including body mass index (BMI) and waist circumference (WC) were measured. BMI was calculated as weight in kilogram divided by height in centimeter square. WC was taken midway between the lowest rib and the iliac crest. Cut off values for anthropometric and lipid profile values were based on the National Cholesterol Education Program-Adult Treatment Panel III (NCEP-ATP III) guidelines. Abnormal lipid profile was defined as TC ≥200 mg/dl, HDL-c < 40mg/dl, LDL-c ≥130 mg/dl, and TAG ≥150mg/dl (19). An elevation of any one of the lipid parameters to a level above these limits was considered as dyslipidemia. Blood pressure was taken by qualified personnel using an analogue sphygmomanometer and stethoscope. Duplicate measurements were taken from the upper arm with the hand at the heart level after the patient had been sitting for more than 5 minutes and the averaged value was recorded. Five milliliter blood sample was collected from the patients by a laboratory technologist for lipid profile investigation after 10–12 hour fasting. Glucose and lipid profile levels were measured by glucose oxidase and direct enzymatic methods, respectively using Bio systems A25 (Costa Brava, Spain) automated clinical chemistry analyzer according to manufacturer's instructions.

Data management and quality control: The questionnaire was pre-tested for accuracy and consistency prior to actual data collection and a half day training was given to data collectors. Furthermore, the principal investigator gave feedback and corrections on daily basis to the data collectors. The completion, accuracy, and clarity of the collected data were checked carefully on a regularly basis. Normal and pathological control samples were run daily in order to check the optimal reactivity of the reagent and functionality of the analyzer. The results obtained from control samples should be within mean ± 2SD of the given Levy Jenning chart. Therefore, to maintain the quality of the result, pre-analytical, analytical and post-analytical precautions of quality, depending on the stated SOP, were considered. Appropriate remedies were taken in cases of unacceptable quality control results. Finally, the sample was processed within 1 hour of specimen collection in the clinical chemistry laboratory and processed based on the manufacturer's manual.

Data analysis and interpretation: Data were entered and analyzed using SPSS version 20 (IBM, USA). The data were tested for normality with the help of histograms, comparison of means and medians, skewness and the Kolmogorov-Smirnov test. Data were reported as mean and standard deviation for continuous variables, percentages for categorical variables and interquartile range for non-normally distributed data. Variables were compared using independent sample t-test for normally distributed data and Mann-Whitney U test for non-normally distributed data. Pearson's and spearman's rank correlations were used to test the relationship of lipid profile with clinical and anthropometric variables. P-value <0.05 was considered statistically significant.

Ethical consideration: Ethical clearance was taken from Research and Ethical Committee of the School of Biomedical and Laboratory Sciences, University of Gondar, and permission was secured from both the Medical Director and the head of the Chronic Illness Clinic of the Hospital. In addition, written informed consent was obtained from the study participants before data collection.

Result

Socio-demographic characteristics of the study participants: A total of 296 (148 T2DM and 148 controls) study participants were participated in this study. Of the total T2DM patients, 59(39.9%) and 89(60.1%) were males, females respectively. Similarly, of the 148 healthy controls 89(60.1%) were females. The overall mean age was 49.09± 8.1 and 47.8± 6.7 years for T2DM and controls respectively. The residence of the majority, 278 (93.9%), of the participants was urban. Summary of the other demographic variables are presented in (Table 1).

Table 1
Socio-demographic characteristics of study participants at University of Gondar Hospital, Northwest Ethiopia, 2015 (N=296).

Clinical features and anthropometric measurements of the study participants: The result showed that the mean levels of BMI, WHR, systolic BP and diastolic BP were significantly higher (P<0.05) in T2DM compared to controls. The median duration of illness since diagnosis was 3 years for T2DM patients. From the total study participants, 72(48.6%) and 35 (23.6%) were overweight cases and controls, respectively. The mean WC showed statistically significant difference between the two groups (83.02±12.6 and 78.67±10.1), p<0.001 respectively (Table 2).

Table 2
Clinical features and anthropometric measurements of the study participants at University of Gondar Hospital, Northwest Ethiopia, 2015 (n=296).

Comparison of lipid profile and Fasting blood sugar level of the study participants: Significant increase in TC, LDL-c, TAG and FBS concentrations were observed in diabetic patients (205.4±50.9 vs 184.9±44.1, 113.1±43.2 vs 100.1±36.4, 189.22± 100.9 vs 115.13±59.2 and 163.7±1.33 vs 81.3 ±1.08 (p<0.05) as compared to the control group respectively. On the contrary, there was a significant decline in mean concentration of HDL-c in T2DM compared to controls (56.5±20.4 vs 62.1±13) p=0.007 respectively (Table 3).

Table 3
Comparison of lipid profiles and Fasting blood sugar concentration of the study participants using t-test at University of Gondar Hospital, Northwest Ethiopia, 2015 (n=296).

The most commonly observed lipid abnormalities in the study was TAG 94(63.5%) followed by raised LDL92 (62.2%), hypercholesterolemia 80(54.1%) and low HDL cholesterolemia 24(16.2%) for T2DM patients. Raised LDL cholesterolemia 76(51.4%) followed by hypercholesterolemia 51(34.5%), hypertriacylglycerolemia 40 (27%) and low HDL cholesterolemia 2(1.4%) were reported in controls. The proportions of lipid profile disorders were higher in DM patients compared to apparently healthy controls (Figure 1).

Figure 1
Proportions of diabetes mellitus patients and controls in relation to lipid profile concentrations at University of Gondar Hospital, Northwest Ethiopia, 2015

The association between CVD risk of lipid parameters effects on type 2 diabetic and non-diabetic participants: The serum lipid profile associated risks were evaluated by using univariate and multivariate logistic regression analysis and summarized in (Table 4). Bivariate logistic regression analysis revealed that the higher lipid values of TC, TAG, HDL cholesterol and LDL cholesterol were significantly (p<0.001) associated with the risk of developing CVD among T2DM and control subjects. Further, adjusted multivariate logistic regression analysis confirmed that TAG (OR=1.015, 95% CI=1.010–1.021, p=0.000) were the significant and independent risk factors for CVD among T2DM patients (Table 4).

Table 4
The association between cardiovascular disease risk and serum lipid concentrations of T2DM and non-diabetic subjects at University of Gondar Hospital, Northwest Ethiopia, 2015

Correlations between lipid profiles with FBS, Duration of DM and Anthropometric measurements of study participants: There was non-significant negative correlation between BMI and HDL, where as a statistically significant positive correlation existed in other lipid profiles in both diabetic and control groups. Waist to hip ratio (WHR) achieved a statistical significant positive correlation with Total cholesterol (p=0.000), Low density lipoprotein (p=0.000) and Triacylglycerol (p=0.015) in T2DM (Table 5).

Table 5
Pearson's correlations(r) between lipid profiles with BMI and WHR among T2DM and healthy controls at University of Gondar Hospital, Northwest, Ethiopia, 2015, (n=296).

The correlation between systolic BP among various lipid profiles showed statistically significant correlation in T2DM and control groups for TAG. However, diastolic BP was not found to have statistically significant correlation with any of the lipid profiles (Table 6). In the T2DM groups, FBS showed a significant positive correlation with TAG and LDL. There was no significant correlation observed among any of lipid profiles in the control group. Duration of T2DM also significantly correlated with Low density lipoprotein and total cholesterol (Table 6).

Table 6
Correlations between lipid profiles with Systolic, diastolic blood pressure, FBS and duration of DM among T2DM and healthy controls at University of Gondar Hospital, Northwest, Ethiopia, 2015, (n=296).

Discussion

This study showed a significant increase in TC, LDL cholesterol and TAG in diabetic patients compared to the control group. In addition, there was a significant decline in mean concentration of HDL cholesterol in T2DM patients compared to controls. These phenomena indicate the occurrence of risk factors for CVD which was documented by several studies in agreement to this study (2733). Hypertriacylglycerolemia (63.5%) was the major lipid parameter disorder in our study. This finding is in concord with previous studies in Hyderabad (60%) and Sudan (48.8%) (28,31). This is due to the fact that increased hepatic secretion of VLDL and delayed clearance of TAG rich lipoproteins increased levels of substrates for TAG production, enhanced mobilization of free fatty acid (FFA) from adipose tissue and increased in the blood of DM patients as observed in the present study (34). Elevated TAG levels are a common dyslipidemic feature accompanying T2DM and prediabetes states (35). A fasting TAG level of greater than 150 mg/dl is one of the accepted criteria for defining individuals at high risk for CVD and T2DM (36).

Our study showed raised TAG, LDL and low HDL cholesterol levels in DM patients. This result is similar to Asian Pacific Cohort Studies Collaboration (37). According to the CDC, 97% of adults with diabetes have one or more lipid abnormalities (38). On contrary to previous studies, TC and LDL cholesterol were raised in our finding (37,29) in T2DM patients. Another study showed that there is no statistically significant difference in serum TC and LDL cholesterol concentration in diabetic patients compared to control groups which is inconsistent to our study (29,32). In contrast to our result, (16.2%), HDL cholesterol dyslipidemia in T2DM is higher in previous studies in Hyderabad (71%), Nepal (20%), Sudan (93%) and Ghana (26%) (27,28,30,31). The variation is due to differences in genetics, life style difference and management of diabetes in the particular group being studied.

Previous study reported that lipid compositions of various tissues altered in diabetes (39). Hence, it is postulated that circulatory lipids play a vital role in progression of T2DM, not only by way of lipid abnormalities but also by modifying the composition, structure and firmness of cellular membranes (39). Several studies reported high levels of TAG, TC and LDL-c among diabetic patients, a finding observed in the present study too. However, another study in Nigeria reported a different finding (33) which is higher TAG in controls. The main cause for lipid abnormalities in T2DM patients is impaired secretion of insulin that affects the liver apolipoprotein production and regulates the enzymatic activity of lipoprotein lipase (LpL) and cholesterol ester transport protein (CETP). Moreover, its deficiency reduces the activity of hepatic lipase; therefore, several steps involved in the production of biologically active LpL might be altered in T2DM compared to controls.

Generally, T2DM is associated with low HDL cholesterol levels, which is an independent cardiovascular risk factor. The low concentrations reflect a dysregulation in HDL metabolism. It is determined by the concerted action of different proteins, including CETP, lecithin cholesterol acyltransferase, LpL, phospholipid transfer protein, hepatic lipase and dysfunctional HDL-mediated reverse cholesterol transport are impaired in T2DM patients compared to controls (40). The association between dyslipidemia and risk of CVD is biologically plausible and compatible with what is known about pathophysiology of CVD. The hypothesized mechanism for dyslipidemia and CVD association has been described. Investigators noted that elevated plasma lipid and lipoprotein may induce endothelial dysfunction secondary to oxidative stress (22. Other mechanism is pathologic process of CVD via dysregulation of LpL resulting in a dyslipidemic lipid profile (19).

In this study, we also tried to correlate lipid profiles with various clinical and anthropometric variables. The variables above the threshold cut off values were found to be predictors of CVD in various populations. In the present study, we observed statistically significant increases in the mean values of BMI and the WC in T2DM participants compared to the controls. Similar findings were observed by other researchers (41,42). In the present study, lipid profile correlated in a comparable manner with BMI and WHR. This finding is consistent with a previous study (43) which showed positive correlation between BMI and WHR with respect to TC, TAG. However, previous study reported that correlation existed only in BMI and VLDL (44).

A recent study, in agreement with this study, demonstrated significant differences in anthropometric variables and lipid profile in T2DM in three different ethnic groups in Malaysia (45). In another recent study in Iranian, it was observed that correlation happened between BMI and TC, LDL cholesterol, TAG in concord to our study (46). Another recent study from North India, agreement to this study, reported positive correlations between WHR, TC, LDL cholesterol in healthy subjects in (47).

Other study in Saudi Arabia and Korean, consistent to this study, reported that there was a positive correlation between BMI with TC, LDL and WHR with TC, LDL respectively, but in contrast to ours, no correlation was seen between BMI and TAG in Saudi Arabia study (48,49). A similar study in Brazil demonstrates an association between the lipid profiles and body adiposity (50).

Interrelation between level of BP and lipid profiles may be through diabetes related to the development of hypertension and dyslipidemia (51, 52). In our study, the mean systolic and diastolic BP is significantly higher in T2DM patients compared to healthy controls. This is due to peripheral resistance which is a major factor influencing diastolic BP, influenced by narrowing of blood vessels in T2DM patients that increases diastolic BP (53). In contrast to our study, a prospective analytical study found a significant positive correlations of TC, LDL cholesterol with BP (54). A study on patients diagnosed as hypertension found that TC and LDL cholesterol were associated closely with level of BP (55). Another recent cross sectional survey on 32004 patients showed that co-existence of high BP and abnormal glucose metabolism was common in Chinese population (56). A study demonstrated an association between hypertension and hypertriglycerolemia with poor glycemic control consistent to our study (57). Noteworthy is the fact that different to previous studies, our results did not show any correlation between BP and lipid profile in T2DM patients except TAG.

In our study, FBS had statistically significant correlation with TAG and LDL cholesterol in T2DM. Congruent to our study, a study in India reported higher TC, TAG and LDL cholesterol in patients who have poor glycemic control (58), in Nepal on T2DM reported that significant correlations with TC, LDL cholesterol, and significant negative correlation with HDL cholesterol (59). In contrast to our study, a study on healthy population in India showed, significant correlation of FBS with TAG, LDL and negative correlation to HDL cholesterol. Similar to our study, no correlation of FBS with TC in control groups (60). Duration of DM achieved significant correlation with LDL cholesterol, TC. Studies in India found that chronicity of T2DM cause higher lipid disturbance (57) which is observed in our study. According to United Kingdom prospective study risk score for CVD (61,62), duration of diabetes was associated with higher incidence of dyslipidemia of TC, LDL cholesterol in agreement to our study.

In conclusion, there was statistically significant difference in lipid profile level between diabetic patients and apparently healthy controls. The study also reported that significant increase in FBS, systolic and diastolic BP, BMI, WC in diabetics than apparently healthy controls. Significant correlation was observed in lipid profiles with anthropometric and clinical variables. This is a reflection of poor glycemic control and life style changes. This suggests a necessity in educating T2DM patients about regular follow-up and appropriate glycemic control to prevent and delay the progression of the disease. Periodic medical checkup and appropriate life style change is recommended for apparently healthy controls to improve the quality of interventions not only to achieve weight loss goals but also to control dyslipidemia and reduce cardiovascular risk. Thus multi-center prospective studies with larger cohort may be needed in the future.

Acknowledgement

We express our deepest gratitude to all of the study participants. We also would like to acknowledge the University of Gondar Hospital laboratory staff members and Mr. Desalew Wudineh for their cooperation during data collection.

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