PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of genannJournal InfoAuthorsPermissionsJournals.ASM.orggenomeA ArticleGenome Announcements
 
Genome Announc. 2017 September; 5(38): e01031-17.
Published online 2017 September 21. doi:  10.1128/genomeA.01031-17
PMCID: PMC5609430

Genome Sequence of Bacillus safensis Strain Ingolstadt Isolated from the Pectoralis Pouch of a Patient with Defibrillator-Related Surgery

ABSTRACT

We report the draft genome sequence of clindamycin-resistant Bacillus safensis strain Ingolstadt isolated from a patient with bacterial colonization after heart surgery. The draft genome comprises 3.75 Mbp and harbors 3,793 predicted protein-encoding genes and a small plasmid.

GENOME ANNOUNCEMENT

The Bacillus pumilus group comprises five species, B. pumilus, B. altitudinis B. aerophilus, B. safensis, and B. stratosphericus (1). This group contains soil bacteria, and some strains of B. pumilus sensu stricto cause severe infections. The wide range of reported cases includes cutaneous lesions, anthrax-like necrosis, catheter infections, and severe sepsis in neonatal infants (2,4). Bacillus safensis strains have been characterized as surfactant producers (5) and as petroleum degraders employing excreted lipolytic exoenzymes (6). Because of the bacterium’s resistance against disinfection with hydrogen peroxide, other strains have been isolated from clean surfaces of spacecraft and their production facilities (7). Here, we report the genome sequence of a bacillus initially identified as B. pumilus by use of matrix-assisted laser desorption ionization time of flight (MALDI-TOF). This isolate turned out to be a B. safensis strain (7) which had caused internal colonization of a patient treated at the general hospital in Ingolstadt, Germany.

B. safensis strain Ingolstadt was isolated from a smear withdrawn on 24 January 2017 from the pectoralis pouch of a defibrillator implanted in a 66-year-old man. The patient had received the defibrillator some weeks earlier. During a second surgery in 2017, the defibrillator was refitted with a new electrode located within the epicardium of the left ventricle. Antimicrobial therapy with cefazolin and rifampin prevented persistence of the bacteria and colonization of the new electrode. No lesions or any other tissue damage was visible, which differs from earlier reports of symptoms associated with infections with closely related B. pumilus sensu stricto in humans (2,4).

Whole-genome shotgun (WGS) sequencing of B. safensis strain Ingolstadt was performed with Illumina MiSeq sequencing technology (Illumina Inc.) using Nextera V3 2- × 300-bp chemistry. High-quality paired-end reads were assembled de novo using an in-house script based on SPAdes assembler (8) and Pilon (9) for genome assembly correction. A total of 3,754,517 bp were assembled in 17 contigs with an N50 value of 954,574 and 41.5% GC content.

A total of 3,793 coding sequences (CDSs) of the draft genome of B. safensis Ingolstadt were annotated using Prokka (10). RNAmmer software (11) was used to predict 5S, 16S, and 23S ribosomal RNAs. Eight copies of 5S, 16S, and 23S rRNA and 72 tRNA loci were identified. The closest genome relative to strain Ingolstadt in the NCBI database was B. safensis strain KCTC (12,796 bp) (GenBank accession number CP018197), with an average sequence identity of 96.6% (12).

B. safensis Ingolstadt harbors a small plasmid (7,241 bp, contig 15) that shares about 97% similarity with B. pumilus strain GR-8 plasmid pGR-8 (accession number CP009109). The plasmid harbors six CDSs but lacks any obvious virulence factors. Antimicrobial susceptibility testing of strain Ingolstadt revealed resistance against the lincosamide antibiotic clindamycin (MIC >4 mg/µl as determined by microdilution assays). No cfr-like genes (13), which are known to confer lincosamide resistance through ribosome methylation, were identified on the genome. Similarly, sequence analysis of the 23S rRNA, rplD (L4 r-protein), and rplC (L3 r-protein) genes of strain Ingolstadt did not show any single-nucleotide variant known to contribute to lincosamide resistance in other Gram-positive bacteria (14).

Accession number(s).

This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number NMQS00000000. The version described in this paper is version NMQS01000000.

ACKNOWLEDGMENTS

We thank Linda Dobrzykowski, Philipp Vette, and Christine Schmidt for skillful technical assistance.

This work was supported by funds from the German Ministry of Defense (Sonderforschungsprojekt 25Z1-S-431214 to M.H.A.).

Footnotes

Citation Dematheis F, Antwerpen MH, Grass G, Walter MC, Borgmann S. 2017. Genome sequence of Bacillus safensis strain Ingolstadt isolated from the pectoralis pouch of a patient with defibrillator-related surgery. Genome Announc 5:e01031-17. https://doi.org/10.1128/genomeA.01031-17.

REFERENCES

1. Liu Y, Lai Q, Dong C, Sun F, Wang L, Li G, Shao Z 2013. Phylogenetic diversity of the Bacillus pumilus group and the marine ecotype revealed by multilocus sequence analysis. PLoS One 8:e80097. doi:.10.1371/journal.pone.0080097 [PMC free article] [PubMed] [Cross Ref]
2. Kimouli M, Vrioni G, Papadopoulou M, Koumaki V, Petropoulou D, Gounaris A, Friedrich AW, Tsakris A 2012. Two cases of severe sepsis caused by Bacillus pumilus in neonatal infants. J Med Microbiol 61:596–599. doi:.10.1099/jmm.0.033175-0 [PubMed] [Cross Ref]
3. Bentur HN, Dalzell AM, Riordan FA 2007. Central venous catheter infection with Bacillus pumilus in an immunocompetent child: a case report. Ann Clin Microbiol Antimicrob 6:12. doi:.10.1186/1476-0711-6-12 [PMC free article] [PubMed] [Cross Ref]
4. Grass G, Bierbaum G, Molitor E, Götte N, Antwerpen M 2016. Genome sequence of Bacillus pumilus strain Bonn, isolated from an anthrax-like necrotic skin infection site of a child. Genome Announc 4(1):e01741-15. doi:.10.1128/genomeA.01741-15 [PMC free article] [PubMed] [Cross Ref]
5. Domingos DF, de Faria AF, de Souza Galaverna R, Eberlin MN, Greenfield P, Zucchi TD, Melo IS, Tran-Dinh N, Midgley D, de Oliveira VM 2015. Genomic and chemical insights into biosurfactant production by the mangrove-derived strain Bacillus safensis CCMA-560. Appl Microbiol Biotechnol 99:3155–3167. doi:.10.1007/s00253-015-6377-8 [PubMed] [Cross Ref]
6. Hanano A, Shaban M, Almousally I 2017. Biochemical, molecular, and transcriptional highlights of the biosynthesis of an effective biosurfactant produced by Bacillus safensis PHA3, a petroleum-dwelling bacteria. Front Microbiol 8:77. doi:.10.3389/fmicb.2017.00077 [PMC free article] [PubMed] [Cross Ref]
7. Satomi M, La Duc MT, Venkateswaran K 2006. Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces. Int J Syst Evol Microbiol 56:1735–1740. doi:.10.1099/ijs.0.64189-0 [PubMed] [Cross Ref]
8. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. doi:.10.1089/cmb.2012.0021 [PMC free article] [PubMed] [Cross Ref]
9. Walker BJ, Abeel T, Shea T, Priest M, Abouelliel A, Sakthikumar S, Cuomo CA, Zeng Q, Wortman J, Young SK, Earl AM 2014. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS One 9:e112963. doi:.10.1371/journal.pone.0112963 [PMC free article] [PubMed] [Cross Ref]
10. Seemann T. 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30:2068–2069. doi:.10.1093/bioinformatics/btu153 [PubMed] [Cross Ref]
11. Lagesen K, Hallin P, Rødland EA, Staerfeldt HH, Rognes T, Ussery DW 2007. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 35:3100–3108. doi:.10.1093/nar/gkm160 [PMC free article] [PubMed] [Cross Ref]
12. Kurtz S, Phillippy A, Delcher AL, Smoot M, Shumway M, Antonescu C, Salzberg SL 2004. Versatile and open software for comparing large genomes. Genome Biol 5:R12. doi:.10.1186/gb-2004-5-2-r12 [PMC free article] [PubMed] [Cross Ref]
13. Hansen LH, Planellas MH, Long KS, Vester B 2012. The order Bacillales hosts functional homologs of the worrisome cfr antibiotic resistance gene. Antimicrob Agents Chemother 56:3563–3567. doi:.10.1128/AAC.00673-12 [PMC free article] [PubMed] [Cross Ref]
14. Stefani S, Bongiorno D, Mongelli G, Campanile F 2010. Linezolid resistance in staphylococci. Pharmaceuticals 3:1988–2006. doi:.10.3390/ph3071988 [PMC free article] [PubMed] [Cross Ref]

Articles from Genome Announcements are provided here courtesy of American Society for Microbiology (ASM)