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Genome Announc. 2017 September; 5(38): e01015-17.
Published online 2017 September 21. doi:  10.1128/genomeA.01015-17
PMCID: PMC5609427

Genomic Sequencing of Ranaviruses Isolated from Edible Frogs (Pelophylax esculentus)

ABSTRACT

Ranaviruses were isolated from wild edible frogs (Pelophylax esculentus) during epizootics in Denmark and Italy. Phylogenomic analyses revealed that these isolates are closely related and belong to a clade of ranaviruses that includes the Andrias davidianus ranavirus (ADRV), common midwife toad ranavirus (CMTV), Testudo hermanni ranavirus (THRV), and pike-perch iridovirus (PPIV).

GENOME ANNOUNCEMENT

The earliest reported occurrence of ranaviral disease in amphibians in Europe resulted in recurrent, low-level mortality in wild-caught edible frogs in a commercial operation in Croatia from 1970 to 1981 (1). Other ranaviral isolates from edible frogs have been obtained from tadpoles collected from the wild during disease outbreaks in Italy in 2002 (REV 282/I02) and from free-living adults collected in Denmark in 2008 (PEV_DK1) (2, 3). Mortality events in Rana temporaria in the United Kingdom in the 1990s (4) and, more recently, in common midwife toads (Alytes obstetricans) in Spain (5) and Pelophylax spp. in The Netherlands (6) have been added to the list of amphibian ranaviral outbreaks in Europe.

The two isolates PEV_DK1 and REV 282/I02 were amplified in epithelioma papulosum cyprini cells until the cytopathic effect was complete, and then the resulting supernatant was clarified and the total nucleic acid was purified from the clarified supernatant, as previously described (7). DNA libraries were prepared using the Nextera XT DNA kit (Illumina), and sequencing was performed using a v3 chemistry 600-cycle kit on an Illumina MiSeq platform. De novo assembly of the paired-end reads in SPAdes (8) produced contiguous consensus sequences of 107,392 bp with G+C content of 55.31% and 107,444 bp with G+C content of 56.03% for PEV_DK1 and REV 282/I02, respectively.

The genomes of the two isolates were annotated using Genome Annotation Transfer Utility (GATU) (9) with Frog virus 3 (GenBank accession no. NC_005946) as the reference genome. Additional putative open reading frames (ORFs) were identified using GenemarkS (10), and gene functions were predicted based on BLASTP searches against the NCBI GenBank non-redundant protein sequence database. A total of 99 putative ORFs were predicted in PEV_DK1 and 101 in REV 282/I02 compared to 101 ORFs in Andrias davidianus ranavirus (ADRV) (Genbank accession no. KC865735), 104 ORFs in common midwife toad ranavirus (CMTV) (GenBank accession no. JQ231222 and KP056312), 75 in Testudo hermanni ranavirus (THRV) (GenBank accession no. KP266741), and 109 in pike-perch iridovirus (PPIV) (GenBank accession no. KX574341). Comparative genomic analyses revealed that these two ranaviruses are closely related, except for a nonsense mutation in a gene encoding a hypothetical protein (orthologous to ORF51 in REV 282/I02) and the absence of a gene encoding a hypothetical protein (orthologous to ORF95 in REV 282/I02) in PEV_DK1. An analysis of locally collinear blocks (LCB) in Mauve (11) revealed that the genomes of PEV_DK1 and REV 282/I02 display the same genome arrangement as ADRV, CMTV, THRV, and PPIV (12). Maximum likelihood phylogenetic analyses based on the concatenated genome-wide LCB alignments revealed that the Italian and Danish ranaviral isolates from edible frogs belong to a clade of ranaviruses that includes ADRV, CMTV, THRV, and PPIV.

The repeated isolation, separated by time and space, of nearly identical ranaviral strains from edible frogs indicates that this species may serve as an important natural host. The detection of closely related strains in Chinese giant salamander (Andrias davidianus), common midwife toad (Alytes obstetricans), Hermann’s tortoise (Testudo hermanni), and pike-perch (Sander lucioperca) underscores the low host specificity of these ranaviruses.

Accession number(s).

The complete genome sequences of PEV_DK1 and REV 282/I02 have been deposited in GenBank under the accession numbers MF538627 and MF538628, respectively.

ACKNOWLEDGMENT

We thank Patrick Thompson for his technical assistance.

Footnotes

Citation Ariel E, Subramaniam K, Imnoi K, Sriwanayos P, Ahasan MS, Olesen NJ, Amedeo M, Toffan A, Waltzek TB. 2017. Genomic sequencing of ranaviruses isolated from edible frogs (Pelophylax esculentus). Genome Announc 5:e01015-17. https://doi.org/10.1128/genomeA.01015-17.

REFERENCES

1. Fijan N, Matasin Z, Petrinec Z, Valpotic I, Zwillenberg L 1991. Isolation of an iridovirus-like agent from the green frog (Rana esculenta L.). Vet Arh 61:151–158.
2. Holopainen R, Ohlemeyer S, Schütze H, Bergmann SM, Tapiovaara H 2009. Ranavirus phylogeny and differentiation based on major capsid protein, DNA polymerase and neurofilament triplet H1-like protein genes. Dis Aquat Organ 85:81–91. doi:.10.3354/dao02074 [PubMed] [Cross Ref]
3. Ariel E, Kielgast J, Svart HE, Larsen K, Tapiovaara H, Jensen BB, Holopainen R 2009. Ranavirus in wild edible frogs Pelophylax kl. esculentus in Denmark. Dis Aquat Organ 85:7–14. doi:.10.3354/dao02060 [PubMed] [Cross Ref]
4. Drury SE, Gough RE, Cunningham AA 1995. Isolation of an iridovirus-like agent from common frogs (Rana temporaria). Vet Rec 137:72–73. doi:.10.1136/vr.137.3.72 [PubMed] [Cross Ref]
5. Balseiro A, Dalton KP, del Cerro A, Marquez I, Cunningham AA, Parra F, Prieto JM, Casais R 2009. Pathology, isolation and molecular characterisation of a ranavirus from the common midwife toad Alytes obstetricans on the Iberian Peninsula. Dis Aquat Organ 84:95–104. doi:.10.3354/dao02032 [PubMed] [Cross Ref]
6. Kik M, Martel A, Sluijs AS, Pasmans F, Wohlsein P, Gröne A, Rijks JM 2011. Ranavirus-associated mass mortality in wild amphibians, The Netherlands, 2010: a first report. Vet J 190:284–286. doi:.10.1016/j.tvjl.2011.08.031 [PubMed] [Cross Ref]
7. Subramaniam K, Toffan A, Cappellozza E, Steckler NK, Olesen NJ, Ariel E, Waltzek TB 2016. Genomic sequence of a ranavirus isolated from short-finned eel (Anguilla australis). Genome Announc 4(4):e00843-16. doi:.10.1128/genomeA.00843-16 [PMC free article] [PubMed] [Cross Ref]
8. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. doi:.10.1089/cmb.2012.0021 [PMC free article] [PubMed] [Cross Ref]
9. Tcherepanov V, Ehlers A, Upton C 2006. Genome Annotation Transfer Utility (GATU): rapid annotation of viral genomes using a closely related reference genome. BMC Genomics 7:150. doi:.10.1186/1471-2164-7-150 [PMC free article] [PubMed] [Cross Ref]
10. Besemer J, Lomsadze A, Borodovsky M 2001. GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Nucleic Acids Res 29:2607–2618. doi:.10.1093/nar/29.12.2607 [PMC free article] [PubMed] [Cross Ref]
11. Darling AC, Mau B, Blattner FR, Perna NT 2004. Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 14:1394–1403. doi:.10.1101/gr.2289704 [PubMed] [Cross Ref]
12. Claytor SC, Subramaniam K, Landrau-Giovannetti N, Chinchar VG, Gray MJ, Miller DL, Mavian C, Salemi M, Wisely S, Waltzek TB 2017. Ranavirus phylogenomics: signatures of recombination and inversions among bullfrog ranaculture isolates. Virology, in press. doi:.10.1016/j.virol.2017.07.028 [PubMed] [Cross Ref]

Articles from Genome Announcements are provided here courtesy of American Society for Microbiology (ASM)