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Genome Announc. 2017 September; 5(38): e00999-17.
Published online 2017 September 21. doi:  10.1128/genomeA.00999-17
PMCID: PMC5609424

Draft Genome Sequence of Lactococcus sp. Strain Rs-Y01, Isolated from the Gut of the Lower Termite Reticulitermes speratus


Here, we report the draft genome sequence of Lactococcus sp. strain Rs-Y01, which was isolated from the gut of a wood-feeding termite. The genome information will facilitate the study of the symbiotic functions of this strain in the termite gut.


The complex microbiota in the gut of termites plays key roles in the digestion of lignocellulose and supply of nutrients (1,3). A previous study on the community structure of bacteria with clone library analysis of PCR-amplified 16S rRNA genes revealed the presence of bacteria in the genus Lactococcus represented by two previously uncharacterized phylotypes, Rs-C68 and Rs-B70, in the gut of the wood-feeding lower termite Reticulitermes speratus (4). We isolated a bacterial strain belonging to the genus Lactococcus, designated strain Rs-Y01, from the gut content of R. speratus. The 16S rRNA gene sequence analysis indicated that strain Rs-Y01 was closely related to Rs-B70, showing 99.9% nucleotide identity.

The genome sequencing of Lactococcus sp. strain Rs-Y01 was performed on the MiSeq platform (Illumina) using a MiSeq version 3 reagent kit (600 cycles). The generated reads were assembled using SPAdes version 3.8.1 (5). A total of 2,949,300 reads were assembled into 20 contigs with an N50 length of 461,990 bp and a largest length of 783,435 bp. This assembly resulted in a draft genome sequence of 2,169,598 bp with a G+C content of 41.3%. A total of 2,099 protein-coding DNA sequences and 46 tRNAs were detected by Prokka (6) and the RAST server (7).

Previous studies of termite gut isolates of lactococci have suggested that they play a role in the fermentation of degradation products of (hemi)cellulose, in the oxygen reduction accompanying the shift from lactate to acetate during fermentation (8), and in the supply of folate, a cofactor essential for acetogenesis from CO2 and H2 and required by acetogenic treponemes in the gut (9). For the last role, the genome annotation indicated the presence of genes for folate biosynthesis: folE, folB, folK, folP, folC, and folA; the folQ gene encoding dihydroneopterin triphosphate diphosphatase was not indicated, although a diphosphohydrolase-like gene was fused in frame with the folE gene. The genome sequence will facilitate further studies on the symbiotic roles of Lactococcus sp. Rs-Y01 in the termite gut.

Accession number(s).

The genome sequence of Lactococcus sp. strain Rs-Y01 has been deposited at DDBJ/GenBank under the accession numbers BEDT01000001 to BEDT01000020.


This work was partially supported by a grant from the Institute of Fermentation, Osaka (to M.Y.) and by Grants-In-Aid for Scientific Research from the Japan Society for the Promotion of Science (no. 16K14797 and 17H01447 to M.O.).


Citation Yuki M, Sakamoto M, Kuwahara H, Hongoh Y, Ohkuma M. 2017. Draft genome sequence of Lactococcus sp. strain Rs-Y01, isolated from the gut of the lower termite Reticulitermes speratus. Genome Announc 5:e00999-17.


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