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Genome Announc. 2017 September; 5(38): e00939-17.
Published online 2017 September 21. doi:  10.1128/genomeA.00939-17
PMCID: PMC5609411

Complete Genome Sequences of emm111 Type Streptococcus pyogenes Strain GUR, with Antitumor Activity, and Its Derivative Strain GURSA1 with an Inactivated emm Gene

ABSTRACT

We present here the complete genome sequence of Streptococcus pyogenes type emm111 strain GUR, a throat isolate from a scarlet fever patient, which has been used to treat cancer patients in the former Soviet Union. We also present the complete genome sequence of its derivative strain GURSA1 with an inactivated emm gene.

GENOME ANNOUNCEMENT

Streptococcus pyogenes is a Gram-positive pathogenic bacterium causing a wide variety of human diseases (1). It has also been used for bacteriolytic cancer therapy starting with “Coley’s toxins” (2) and followed by OK-432 (picibanil) (3), but the mechanisms behind its antitumor activity are unknown. S. pyogenes strain GUR, isolated from a scarlet fever patient in the former Soviet Union in 1938, has been used clinically for anticancer treatment in the former Soviet Union for more than 20 years (4) and showed therapeutic effects in several murine cancer models (M. Suvorova, E. P. Kiseleva, and A. N. Suvorov, unpublished data). To identify the role of the antiphagocytic M protein in the cytotoxic activity of this strain, we generated a mutant (strain GURSA1) by inactivating the emm gene by insertional mutagenesis (5). Unexpectedly, strain GURSA1 showed better cytotoxic activity in both in vitro and in vivo experiments on murine malignant tumor cells (M. Suvorova, E. P. Kiseleva, and A. N. Suvorov, unpublished data). To understand the mechanisms of how strains GUR and GURSA1 affect tumor cells, we sequenced their genomes.

Genomic DNA was extracted from overnight cultures of S. pyogenes strains GUR and GURSA1 using the MagAttract pathogen kit (Qiagen, USA). Fragment libraries were prepared using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA), followed by 251-bp paired-end sequencing on a MiSeq instrument (Illumina), which This generated 496 Mb and 234 Mb of high-quality paired-end sequence data for strains GUR and GURSA1, respectively.

We first assembled the genome of strain GURSA1 using a custom-built SPAdes assembler version 3.10.0 (6), with the “-k 55,77,99,121,143,151,155” option to specify iterative k-mers. For scaffolding, we used full chromosome sequences from three closely related S. pyogenes strains (ATCC 19615, AP1, and AP53, with GenBank accession numbers NZ_CP008926, NZ_CP007537, and NZ_CP013672, respectively) using the “--untrusted-contigs” option. The resulting single scaffold was improved using Pilon version 1.22 software (7). We circularized this gapless chromosome sequence and rearranged it to start at the dnaA gene using the Circlator software (8). We then assembled the genome of strain GUR using an identical procedure, with the following modifications: different k-mers with “-k 33,55,77,99,121,143” and providing the chromosome sequence of GURSA1 with the “--untrusted-contigs” option. The resulting single scaffold was also improved, circularized, and rearranged. The genomes of strains GUR and GURSA1 consisted of 1,890,204 bp and 1,893,171 bp, respectively. They encoded 1,820 and 1,825 proteins, respectively, predicted by NCBI’s Prokaryotic Genome Annotation Pipeline (9). Both genomes had 38.5% G+C content, harbored 67 tRNA genes, and encoded 6 copies of the rRNA operon. Using the PHASTER online tool (10), we identified five intact prophage regions with high homology to prophage regions from M3 serotype S. pyogenes strain MGAS315. Other than the genomic changes introduced during mutagenesis, GUR and GURSA1 had four single-nucleotide changes between them. Three of these changes were synonymous mutations within the third prophage region, and the fourth change was a nonsynonymous (A284V) change in the Hpr kinase/phosphorylase gene. We assigned type emm111 to strain GUR using the instructions provided by the Centers for Disease Control and Prevention’s Streptococcus Laboratory (https://www.cdc.gov/streplab/).

Accession number(s).

The genome sequences of strains GUR and GURSA1 were deposited in GenBank under the accession numbers CP022354 and CP022206, respectively. The versions described in this paper are the first versions.

ACKNOWLEDGMENTS

This work has been funded by the Novo Nordisk Foundation grant NNF10CC1016515.

We thank Statens Serum Institut, Denmark, for assistance in genome sequencing.

Footnotes

Citation Suvorova MA, Tsapieva AN, Bak EG, Chereshnev VA, Kiseleva EP, Suvorov AN, Arumugam M. 2017. Complete genome sequences of emm111 type Streptococcus pyogenes strain GUR, with antitumor activity, and its derivative strain GURSA1 with an inactivated emm gene. Genome Announc 5:e00939-17. https://doi.org/10.1128/genomeA.00939-17.

REFERENCES

1. Walker MJ, Barnett TC, McArthur JD, Cole JN, Gillen CM, Henningham A, Sriprakash KS, Sanderson-Smith ML, Nizet V 2014. Disease manifestations and pathogenic mechanisms of group A Streptococcus. Clin Microbiol Rev 27:264–301. doi:.10.1128/CMR.00101-13 [PMC free article] [PubMed] [Cross Ref]
2. Starnes CO. 1992. Coley’s toxins in perspective. Nature 357:11–12. doi:.10.1038/357011a0 [PubMed] [Cross Ref]
3. Ryoma Y, Moriya Y, Okamoto M, Kanaya I, Saito M, Sato M 2004. Biological effect of OK-432 (picibanil) and possible application to dendritic cell therapy. Anticancer Res 24:3295–3301. [PubMed]
4. Chereshnev VA, Morova AA, Ryamzina I 2006. Биологические законы и жизнеспособность человека: метод многофункциональной восстановительной биотерапии, 2nd ed Perm State Agricultural Academy Press, Perm, Russia.
5. Suvorova MA, Tsapieva AN, Duplik NV, Kramskoy TA, Grabovskaya KB, Kiseleva EP, Chereshnev VA, Suvorov AN 2016. Конструирование штамма стрептококка, мутантного по гену М-белка. Meditsinskiy Akademicheskiy Zhurnal 16:235–236.
6. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. doi:.10.1089/cmb.2012.0021 [PMC free article] [PubMed] [Cross Ref]
7. Walker BJ, Abeel T, Shea T, Priest M, Abouelliel A, Sakthikumar S, Cuomo CA, Zeng Q, Wortman J, Young SK, Earl AM 2014. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS One 9:e112963. doi:.10.1371/journal.pone.0112963 [PMC free article] [PubMed] [Cross Ref]
8. Hunt M, Silva ND, Otto TD, Parkhill J, Keane JA, Harris SR 2015. Circlator: automated circularization of genome assemblies using long sequencing reads. Genome Biol 16:294. doi:.10.1186/s13059-015-0849-0 [PMC free article] [PubMed] [Cross Ref]
9. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP, Zaslavsky L, Lomsadze A, Pruitt KD, Borodovsky M, Ostell J 2016. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 44:6614–6624. doi:.10.1093/nar/gkw569 [PMC free article] [PubMed] [Cross Ref]
10. Arndt D, Grant JR, Marcu A, Sajed T, Pon A, Liang Y, Wishart DS 2016. PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res 44:W16–W21. doi:.10.1093/nar/gkw387 [PMC free article] [PubMed] [Cross Ref]

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