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Genome Announc. 2017 August; 5(33): e00805-17.
Published online 2017 August 17. doi:  10.1128/genomeA.00805-17
PMCID: PMC5604776

Draft Genome Sequence of Staphylococcus aureus S681, a Tetracycline-Sensitive Livestock-Associated Methicillin-Resistant Clonal Complex 398 Strain

ABSTRACT

We present the draft genome sequence of an atypical tetracycline-susceptible livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) strain. It contains 2,817,340 bp and 2,858 coding sequences, including 6 rRNA operons, 56 tRNAs, and 4 noncoding RNA (ncRNA) genes. The strain harbors a tet(M) gene, but 15 point mutations in amino acids are present that likely impair the functionality of TetM.

GENOME ANNOUNCEMENT

Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of nosocomial infections and a major public health concern. Livestock-associated methicillin-resistant Staphylococcus aureus characteristically harbors not only mecA, conferring resistance to beta lactams, but also the tetracycline resistance determinant tet(M), alone or in combination with tet(K) and/or tet(L) (1, 2). In this study, we present the draft genome sequence of S. aureus S681, a tetracycline-susceptible livestock-associated MRSA. The strain was isolated from a pig carcass from a slaughterhouse in Switzerland and was shown to belong to spa type t034 and clonal complex 398 (CC398) (3). The strain exhibited resistance to ampicillin, cefoxitin, oxacillin, and penicillin in a disk diffusion assay. While the strain showed a tetracycline-susceptible phenotype, a DNA microarray assay yielded a positive hybridization result for a probe targeting tet(M) (4).

In this study, the genome of strain S681 was sequenced using Ilumina Miseq 125-bp paired-end sequence technology as described previously (5). The raw reads were paired in CLC genomics workbench 9.0, resulting in 1,560,556 reads with a mean size of 250 bp. The reads were assembled in CLC genomics workbench 9.0 using standard settings, including scaffolding. The assembly was corrected by NCBI PGAP. The final version contains 30 contigs with sizes between 1.5 and 440 kbp. The average read coverage of the genome was 120-fold.

The genome of S. aureus S681 contains 2,817,340 bp and 2,858 coding sequences, including 6 rRNA operons, 56 tRNAs, and 4 noncoding RNA (ncRNA) genes. The G+C content of the genome is 32.3%.

Remarkably, the tetracycline resistance gene tet(M) (locus CDQ86_05625) was detected in the genome of this phenotypically tetracycline-susceptible strain. However, 15 point mutations in amino acids were present, which quite likely impair the functionality of TetM.

Accession number(s).

This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number NISC00000000. The version described in this paper is version NISC01000000.

Footnotes

Citation Stevens MJA, Stephan R, Johler S. 2017. Draft genome sequence of Staphylococcus aureus S681, a tetracycline-sensitive livestock-associated methicillin-resistant clonal complex 398 strain. Genome Announc 5:e00805-17. https://doi.org/10.1128/genomeA.00805-17.

REFERENCES

1. Heikinheimo A, Johler S, Karvonen L, Julmi J, Fredriksson-Ahomaa M, Stephan R 2016. New dominant spa type t2741 in livestock-associated MRSA (CC398-MRSA-V) in Finnish fattening pigs at slaughter. Antimicrob Resist Infect Contr 5:6. doi:.10.1186/s13756-016-0105-8 [PMC free article] [PubMed] [Cross Ref]
2. Argudín MA, Tenhagen BA, Fetsch A, Sachsenröder J, Käsbohrer A, Schroeter A, Hammerl JA, Hertwig S, Helmuth R, Bräunig J, Mendoza MC, Appel B, Rodicio MR, Guerra B 2011. Virulence and resistance determinants of German Staphylococcus aureus ST398 isolates from nonhuman sources. Appl Environ Microbiol 77:3052–3060. doi:.10.1128/AEM.02260-10 [PMC free article] [PubMed] [Cross Ref]
3. Huber H, Koller S, Giezendanner N, Stephan R, Zweifel C 2010. Prevalence and characteristics of meticillin-resistant Staphylococcus aureus in humans in contact with farm animals, in livestock, and in food of animal origin, Switzerland, 2009. Euro Surveill 15:16 pii: 19542 http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19542. [PubMed]
4. Huber H, Giezendanner N, Stephan R, Zweifel C 2011. Genotypes, antibiotic resistance profiles and microarray-based characterization of methicillin-resistant Staphylococcus aureus strains isolated from livestock and veterinarians in Switzerland. Zoonoses Publ Health 58:343–349. doi:.10.1111/j.1863-2378.2010.01353.x [PubMed] [Cross Ref]
5. Stevens MJ, Stephan R, Johler S 2017. Draft genome sequence of Staphylococcus aureus 1608, a strain that caused toxic mastitis in twin cows. Genome Announc 5(1):e01438-16. doi:.10.1128/genomeA.01438-16 [PMC free article] [PubMed] [Cross Ref]

Articles from Genome Announcements are provided here courtesy of American Society for Microbiology (ASM)