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Genome Announc. 2017 August; 5(33): e00756-17.
Published online 2017 August 17. doi:  10.1128/genomeA.00756-17
PMCID: PMC5604765

Draft Genome Sequences of Two Enterobacter cloacae subsp. cloacae Strains Isolated from Australian Hematology Patients with Bacteremia


Enterobacter cloacae is a common member of the gut microbiota in healthy individuals. However, it is also an opportunistic pathogen, capable of causing bacteremia. We report the draft genomes of two Enterobacter cloacae subspecies cloacae strains isolated from hematology patients with bacteremia. Both isolates carry genes encoding extended-spectrum β-lactamases.


Bacteria of the Enterobacter cloacae complex are commensal enteric bacteria of the human gastrointestinal tract. In recent years, these bacteria have taken on clinical significance as opportunistic pathogens that cause severe bacteremia through endogenous translocation from the host gut (1,3). E. cloacae and other Enterobacter spp. have intrinsic and inducible resistance against broad-spectrum cephalosporins through chromosomal AmpC β-lactamase (1). Enterobacter strains are also capable of acquiring plasmids that encode multiple drug resistance mechanisms, such as extended-spectrum β-lactamases (ESBL) that hydrolyze a broader range of cephalosporins, including fourth-generation cephalosporins (cefepime) (4,7). Infections by these multidrug-resistant strains pose a significant therapeutic challenge.

We report the draft genome sequences and antibiotic resistance gene profiles of two Enterobacter cloacae subsp. cloacae isolates (49530189 and 60830776) from the Enterobacter cloacae complex, which were isolated from two different individuals with hospital-acquired bacteremia. Both isolates were obtained by blood culture, and their antibiotic susceptibilities were determined using EUCAST breakpoints with the BD-Pheonix automated platform (Becton, Dickinson, NJ, USA) and double-disc testing. Isolate 49530189 was determined to be resistant to ciprofloxacin, co-trimoxazole, cefepime, and other β-lactams. Isolate 60830776 differed slightly, being intermediately resistant to cefepime and resistant to ciprofloxacin, co-trimoxazole, aztreonam, gentamicin, and tobramycin.

Genomic DNA was isolated using an EZ-10 Spin genomic DNA kit (Bio Basic Canada, Inc., Ontario, Canada). Whole-genome amplicon libraries were prepared using an Illumina Nextera XT DNA sample preparation kit (Illumina, Inc., CA) and sequenced on an Illumina NextSeq platform with a NextSeq 500/550 mid-output kit (v2 Illumina) (2 × 150-bp cycles).

A total of 3,066,602, and 1,876,696 paired-end reads for isolates 49530189 and 60830776, respectively, were mapped to the Enterobacter cloacae subsp. cloacae ATCC 13047 reference genome (GenBank accession no. NC_014121), yielding average coverages of 78-fold and 49-fold, respectively. Downstream processing was performed using the Nullarbor pipeline (see The draft genome of isolate 49530189 consisted of 170 contigs, with a total of 5,467,426 bp and G+C content of 54.8%. The draft genome of isolate 60830776 consisted of 140 contigs, with a total of 5,168,633 bp and G+C content of 54.6%. Isolates 49530189 and 60830776 have 5,239, and 4,879 coding domain sequences, respectively. Both draft genomes have six sets of rRNA genes. The core genome between the 2 isolates consists of 2,332 genes.

While the two isolates have differing profiles of antibiotic resistance genes, both carry genes that confer resistance to aminoglycosides, β-lactams, fosfomycin, olaquindox, quinolone, sulfonamide, and tetracycline. However, isolate 60830776 also harbors resistance genes toward chloramphenicol (catB3), and macrolide (mphA). The two isolates are AmpC negatve and ESBL positive. Isolate 49530189 carries AmpC (blaACT-9) and CTX-M (blaCTX-M-9) β-lactamases, while isolate 60830776 harbors AmpC (blaACT-16), TEM-1 (blaTEM-1B), SHV (blaSHV−12), and OXA-1 (blaOXA-1) β-lactamases. These E. cloacae draft genomes are a useful resource that enables us to better understand antibiotic resistance mechanisms, phenotype-genotype correlations, and evolutionary history.

Accession number(s).

These whole-genome shotgun projects have been deposited in DDBJ/ENA/GenBank under the accession numbers NJCZ00000000 (49530189) and NJDA00000000 (60830776). The versions described here are the first versions.


We acknowledge financial support for this project from SA Pathology and SAHMRI.


Citation Leong LEX, Shaw D, Papanicolas L, Lagana D, Bastian I, Rogers GB. 2017. Draft genome sequences of two Enterobacter cloacae subsp. cloacae strains isolated from Australian hematology patients with bacteremia. Genome Announc 5:e00756-17.


1. Sanders WE, Sanders CC 1997. Enterobacter spp.: pathogens poised to flourish at the turn of the century. Clin Microbiol Rev 10:220–241. [PMC free article] [PubMed]
2. Davin-Regli A, Pagès JM 2015. Enterobacter aerogenes and Enterobacter cloacae; versatile bacterial pathogens confronting antibiotic treatment. Front Microbiol 6:392. doi:.10.3389/fmicb.2015.00392 [PMC free article] [PubMed] [Cross Ref]
3. Keller R, Pedroso MZ, Ritchmann R, Silva RM 1998. Occurrence of virulence-associated properties in Enterobacter cloacae. Infect Immun 66:645–649. [PMC free article] [PubMed]
4. Lee NY, Lee CC, Li CW, Li MC, Chen PL, Chang CM, Ko WC 2015. Cefepime therapy for monomicrobial Enterobacter cloacae bacteremia: unfavorable outcomes in patients infected by cefepime-susceptible dose-dependent isolates. Antimicrob Agents Chemother 59:7558–7563. doi:.10.1128/AAC.01477-15 [PMC free article] [PubMed] [Cross Ref]
5. Lavigne JP, Bouziges N, Chanal C, Mahamat A, Michaux-Charachon S, Sotto A 2004. Molecular epidemiology of Enterobacteriaceae isolates producing extended-spectrum beta-lactamases in a French hospital. J Clin Microbiol 42:3805–3808. doi:.10.1128/JCM.42.8.3805-3808.2004 [PMC free article] [PubMed] [Cross Ref]
6. Reuland EA, Al Naiemi N, Kaiser AM, Heck M, Kluytmans JA, Savelkoul PH, Elders PJ, Vandenbroucke-Grauls CM 2016. Prevalence and risk factors for carriage of ESBL-producing Enterobacteriaceae in Amsterdam. J Antimicrob Chemother 71:1076–1082. doi:.10.1093/jac/dkv441 [PMC free article] [PubMed] [Cross Ref]
7. Nordmann P, Naas T, Poirel L 2011. Global spread of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 17:1791–1798. doi:.10.3201/eid1710.110655 [PMC free article] [PubMed] [Cross Ref]

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