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ACS Chemical Biology
 
ACS Chem Biol. 2017 September 15; 12(9): 2335–2344.
Published online 2017 July 19. doi:  10.1021/acschembio.7b00389
PMCID: PMC5603899

Structure–Activity Relationships Reveal Key Features of 8-Oxoguanine: A Mismatch Detection by the MutY Glycosylase

Abstract

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Base excision repair glycosylases locate and remove damaged bases in DNA with remarkable specificity. The MutY glycosylases, unusual for their excision of undamaged adenines mispaired to the oxidized base 8-oxoguanine (OG), must recognize both bases of the mispair in order to prevent promutagenic activity. Moreover, MutY must effectively find OG:A mismatches within the context of highly abundant and structurally similar T:A base pairs. Very little is known about the factors that initiate MutY’s interaction with the substrate when it first encounters an intrahelical OG:A mispair, or about the order of recognition checkpoints. Here, we used structure–activity relationships (SAR) to investigate the features that influence the in vitro measured parameters of mismatch affinity and adenine base excision efficiency by E. coli MutY. We also evaluated the impacts of the same substrate alterations on MutY-mediated repair in a cellular context. Our results show that MutY relies strongly on the presence of the OG base and recognizes multiple structural features at different stages of recognition and catalysis to ensure that only inappropriately mispaired adenines are excised. Notably, some OG modifications resulted in more dramatic reductions in cellular repair than in the in vitro kinetic parameters, indicating their importance for initial recognition events needed to locate the mismatch within DNA. Indeed, the initial encounter of MutY with its target base pair may rely on specific interactions with the 2-amino group of OG in the major groove, a feature that distinguishes OG:A from T:A base pairs. These results furthermore suggest that inefficient substrate location in human MutY homologue variants may prove predictive for the early onset colorectal cancer phenotype known as MUTYH-Associated Polyposis, or MAP.

Routine oxidative damage due to cellular processes is known to include the common guanine oxidation product 8-oxo-7,8-dihydroguanine (OG).1,2 With only two more atoms than canonical guanines, the OG lesion can be interpreted by polymerases correctly as a G, or incorrectly as a T (Figure Figure11A). In response to this dual coding effect, cells have evolved a context-dependent base excision repair (BER) system to address the OG lesion, known as the “GO repair pathway” (Figure Figure11B).35 The E. coli DNA glycosylase Fpg is responsible for removing the OG lesion when paired opposite C, while MutY removes miscoding adenines opposite the lesion. In this pathway, MutY and its homologues provide a “failsafe” mechanism for OG glycosylases like Fpg, acting as a final barrier to irreparable mutations caused by OG. The importance of MutY’s activity is demonstrated by near-universal homologue conservation from prokaryotes to eukaryotes, and by its disease-relevance in humans as exemplified by an inherited colorectal cancer syndrome known as MUTYH-associated polyposis, or MAP.2,57

Figure 1
Pairing behavior and repair of OG. (A) OG is found paired to both C and A in DNA. (B) Repair of OG lesions is mediated via the GO Repair Pathway.

MutY displays many commonalities with other glycosylases and DNA-binding proteins, along with important differences that set it apart. Along with a growing number of glycosylases, MutY contains a [4Fe–4S]2+ cofactor that is required for activity.711 MutY enzymes are also distinct from other BER glycosylases in possessing a unique C-terminal domain (CTD) that is highly homologous to the NUDIX d(OG)TP hydrolase NUDT1; moreover, the CTD has been shown to be crucial for OG recognition and repair.12,13 Structural insights into the lesion recognition process have been provided by several crystal structures using either a cleavage-resistant 2′-deoxy-2′-fluoroadenosine analog, an inactive enzyme, or a transition state mimic to capture a glimpse of MutY on the cusp of catalysis.1517 At the late stage visualized in these structures, the OG:A bp has been disrupted, and the adenine has been extruded from the helix and placed into an extrahelical pocket where catalysis occurs (Figure Figure22).2,1719 A Tyr residue is inserted between OG and its 5′ neighbor, suggesting a role in disrupting the OG:A bp and stabilizing the severe kink in the DNA.1517,20 Notably, the CTD makes contact with the OG lesion, which has shifted in conformation from OGsyn when paired to A, to OGanti when MutY-bound.13,15,16 Such dramatic changes in DNA conformation (Figure Figure22) could potentially provide multiple checkpoints for the enzyme to achieve its remarkable substrate specificity. MutY, like many DNA binding proteins, is known to utilize a processive search mechanism along DNA;21 however it is currently unknown how MutY effectively locates and discriminates OG:A pairs from other A-containing pairs such as T:A or G:A during this search process. The task required of MutY is daunting when considering the rarity of OG:A pairs compared to T:A pairs in a cellular context.

Figure 2
Dramatic conformational changes between unbound and MutY-bound, catalytically ready substrate DNA. (A) Side views of substrate OG (purple) paired to A (yellow) and nonsubstrate T (green):A (yellow) pairs illustrate the lack of major helix deformation ...

In this work, we aimed to use structure–activity relationships to help elucidate the interactions of MutY with the OG substrate base. We have used a range of experimental techniques to examine specific aspects of MutY’s reaction, from substrate binding, to catalysis, to participation in cellular repair. Glycosylase assays, under single- or multiple-turnover conditions, were used to isolate the rates of catalysis and product release, respectively. Gel shift assays with a binding-competent but catalytically inactive mutated enzyme were employed to determine binding efficiency. A bacterial cell-based assay allowed us to contrast in vitro enzyme behavior with the efficiency of MutY-mediated repair under the more demanding conditions of living cells. By using analogs of the OG base in all of these experiments, we have correlated structural features of OG with their effects on binding, catalysis, product release, and overall repair efficiency in E. coli cells. Our results indicate that the identity of the OG lesion is confirmed by multiple interactions on both the Watson–Crick and Hoogsteen faces of the OG base. Additionally, the results herein support the idea that MutY relies on the presence of the OG lesion first to efficiently select miscoding adenines and then to help catalyze the remote cleavage of the adenine base from DNA. Most strikingly, our results suggest that the initial recognition of an OG:A mispair may occur through a single interaction of MutY with the intrahelical OG base.

Results and Discussion

Choice of Oxoguanine Analogs to Investigate Substrate Recognition Requirements

A series of purine and deazapurine nucleotides (Figure Figure33) were chosen that represent relatively minor changes to the OG base on either the pyrimidine or the imidazole ring. These analogs were incorporated into oligomeric DNA and paired opposite an adenine base in the complementary strand to mimic MutY’s natural OG:A substrate. The choice of specific analog bases was informed by a number of structural and biochemical studies of MutY and other DNA glycosylases.15,16,2225 In the majority of cases, appropriate analogs were not commercially available; thus the choice of analogs was also informed by practical considerations such as synthetic feasibility and potential structural instability.

Figure 3
Analogs of OG used in this study and their electrostatic potential surfaces. For visual clarity and simplicity, only the free nucleobase of each analog was used for electrostatic potential modeling.

Analogs of OG were included that display reduced Watson–Crick complementarity to cytosine (1MOG, 8OA); these analogs were expected to retain the ability to base pair with A but would be expected to interfere with late-stage OG recognition contacts that are observed in MutY-lesion crystal structures (Figure Figure22D).1517 Whereas 1-methyl-8-oxo-7,8-dihydroguanine (1MOG) provides a simple steric block to pairing at one location on the Watson–Crick face, 8-oxo-7,8-dihydroadenine (8OA) retains only the imidazole ring features of OG and instead presents the Watson–Crick binding face of an adenine.26 Notably, 8OA:A pairs are substantially more destabilized than OG:A pairs, comparable to an A:A or C:A mismatch, and MutY has been observed to have weak activity toward adenines in this pairing context.24,26

To investigate the impact of altering the Hoogsteen face of OG, we incorporated changes that would alter H-bonding to A or disfavor the syn conformation (G, 9ZG, 8AG, 8BG, 7MOG, 8OA). Each of these analogs could increase the rate of base flipping by locally destabilizing the region near the introduced base pair; however, disfavoring syn pairing with A may also alter the proper groove placement of base pair recognition elements within duplex DNA. In addition, altering the NH7 donor of OG would also be anticipated to alter late-stage H-bonding interactions observed in the MutY-lesion structures (Figure Figure22). 7-Methyl-8-oxo-7,8-dihydroguanine (7MOG) sterically impedes pairing to A with a methyl group that blocks effective hydrogen bonding, while 8-aminoguanine (8AG) and 8-bromoguanine (8BG) lack a hydrogen bond donor at N7.27,28 8OA preserves the Hoogsteen face but replaces a hydrogen bond acceptor with a donor at the 6-position of the purine.26 Both G and 9-deazaguanine (9ZG) lack the 8-substituent that drives the shift from predominantly anti to predominantly syn conformation.29 However, 9ZG:A pairs are more stable to thermal denaturation than G:A mismatches because 9ZG retains a hydrogen bond donor at N7 that can help stabilize the Hoogsteen pairing to A.30

Two analogs were also included to act as relatively close mimics of OG in terms of base pairing and coding during DNA replication. 8-Thioguanine (8SG) displays an increase in the bulk of the 8-substituent along with a mild decrease in that substituent’s electronegativity; this analog shows similar conformational and base pairing proclivities to OG.30 The base of 8-oxoinosine (8OI) lacks the exocyclic amine of OG, producing a Watson–Crick face that typically codes like G under experimental conditions.31 Likewise, duplexes containing 8SG:A and 8OI:A pairs have similar duplex stabilities to those harboring OG:A pairs.30

Rates of Adenine Cleavage (k2)

We evaluated the ability of wild type E. coli MutY to excise adenine opposite the chosen analogs using a PAGE-based glycosylase assay with a radiolabeled 30 base pair substrate duplex. Under single turnover conditions ([E] > [DNA]), substrate binding is rapid, and the observed rate of product formation corresponds to the rate of catalysis, k2 (Scheme 1). Briefly, the A-containing strand was 5′-radiolabeled and annealed to a complement strand to produce Duplex 1 with a central target X:A base pair, where X is OG or an analog (Figure Figure44A). At defined time points, sodium hydroxide was used to quench the enzymatic reaction and cleave the abasic site product to provide a 14-nucleotide product fragment. The substrate and product oligonucleotides (denoted in Figure Figure44B as DNAS and DNAP, respectively) were separated by denaturing polyacrylamide gels and quantitated following storage phosphor autoradiography. The production curves were fitted to the appropriate rate equation.25

Figure 4
DNA duplexes and experimental determination of glycosylase activity. (A) Duplexes used in this work. (B) Schematic representation of the glycosylase assay for kinetic characterization of MutY’s enzymatic behavior with substrate analog pairs. The ...
Scheme 1
Minimal Kinetic Scheme for the Reaction of E. coli MutY with OG:A Pairs

Whereas MutY cleaves adenine from OG:A pairs at a rate of 12 ± 1 min–1, the enzyme displayed widely variable ability to cleave adenines opposite the analogs studied (Figure Figure55A). Observed glycosylase rates (Table 1) ranged from little or no decrease in k2, as with 8SG:A (10 ± 3 min–1) pairs and 8OI:A (6 ± 1 min–1), to extremely poor cleavage, as with 1MOG:A pairs (0.014 ± 0.003 min–1). Notably, MutY was able to achieve complete conversion of the substrate to product with most of the analog-containing duplexes under these conditions; only the slowest reactions (1MOG:A, 8AG:A, and 9ZG:A) were unable to reach completion in hour-long experiments. Sulfur is a competent mimic of O8 in 8SG:A pairs in terms of MutY-catalyzed excision, while other 8-substituents resulted in reduced activity. The presence of an 8-substituent that favors the syn conformation is not enough to support high activity as indicated by the reduced k2 values for 8BG:A and 8AG:A (60- and 750-fold) substrates. Notably, the 8BG:A and G:A pairs are similarly processed, further underscoring the importance of an oxo-like functional group at this position. A feature of the 8-oxo group in OG is that it demands the presence of an NH at N7, and this facilitates the favorable pairing to A. Our results indicate that the presence of an NH at this position alone is insufficient to support efficient catalysis; indeed, 9ZG:A pairs were among the least efficiently processed. In addition, blocking the N-7 position with a methyl group but retaining the 8-oxo-substituent in 7MOG:A substrates resulted in only a 20-fold reduced efficiency of adenine excision, underscoring the influence of the 8-oxo group on MutY-catalyzed excision.

Figure 5
In vitro product accumulation and binding curves. (A) Representative fits of adenine cleavage from X:A pairs by MutY under single turnover conditions. Differences in final amplitude (cf. 8SG:A vs OG:A) were determined to be due to experiment-to-experiment variation. ...
Table 1
Substrate Dissociation Constants, Catalytic Rates and Product Release Rates of MutY DNA Glycosylase with Various OG Analogs Paired to A

Surprisingly, the individual change that had the most dramatic impacts on adenine cleavage resulted from capping N1 with a methyl group (1MOG:A, 0.014 ± 0.003 min–1). The change at N1 was more deleterious than removal of O8 that is considered the hallmark of OG. The poor activity of MutY on 1MOG:A substrates may result not only from the loss of N1H but from the methyl group preventing proper engagement in the OG binding site. 8OA:A pairs also lack an exchangeable proton at N1 but were somewhat better substrates for MutY (0.13 ± 0.05 min–1). This may be due to favorable effects of the presence of the 8-oxo-substituent, in conjunction with the ability of the N6 hydrogen bond donor of 8OA to replace a lost interaction between OG’s N1–H and the backbone oxygen of Gln42 in the final stages of catalysis (homologous to G. stearothermophilus Gln48 in Figure Figure22D). Interestingly, the effects at different positions are not isolated nor directly correlated. For instance, G lacks both O8 and H7, yet adenine was cleaved faster from G:A pairs than from those containing 9ZG, which lacks only O8 but shows severely compromised cleavage (0.031 ± 0.008 min–1).

Rates of Abasic Site Product Release (k3)

When the glycosylase assay (Figure Figure44B) is performed under multiple turnover conditions with the natural substrate, MutY is strongly inhibited by its apurinic (AP) site product.25 The half-life of the complex between MutY and an OG:AP-containing duplex is greater than 3 h, which is observed experimentally as an initial “burst” of product followed by an extremely slow steady-state rate. This biochemical behavior allows the separation of the chemistry rate from the product release rate by simple changes in relative concentrations of enzyme and substrate. We therefore investigated whether each X:A pair displayed a slower steady-state rate of product formation following the initial burst of AP site formation, similar to OG:A pairs. Only 8SG, 8OI, and 7MOG displayed product release rates that were similar to the native substrate (Table 1). With 8OA:A substrates, the burst is subtle, but defined enough to determine a product release rate of 0.02 ± 0.01 min–1, indicating the importance of both the Watson–Crick and Hoogsteen face of OG for high affinity for the product. The other analogs lacked a detectable burst phase due to their relatively slow catalytic rates, similar to G:A pairs.1,25 Notably, the presence of an 8-oxo-like substituent impacts both efficient adenine excision (k2), as well as the high affinity for the product leading to a small product release rate k3.

Substrate Binding Prior to Catalysis

Because the chemistry step of adenine cleavage is relatively fast and MutY binds tightly to abasic sites, standard electrophoretic mobility shift assays (EMSA) with radiolabeled DNA and wild-type MutY result in a measurement that reflects a mixture of enzyme–substrate and enzyme–product complexes. A convenient strategy for evaluating relative substrate affinity by MutY involves stalling catalysis by using either a cleavage-resistant substrate or a catalytically inactive mutated MutY. Although cleavage-resistant 2′-deoxyadenosine analogs can be used for this purpose, we felt that the use of analogs in place of both nucleotides in the substrate pair would complicate our analyses. We chose instead to use catalytically inactive E37S MutY that has been well characterized by our laboratory.1,32 In E37S MutY, the glutamic acid residue that initiates glycosidic bond cleavage by protonating the scissile adenine at N7 has been replaced with serine; notably, this enzyme form retains high affinity and differential binding for specific and nonspecific DNA, similar to the wild type protein. Importantly, we observed complete binding of all of the analog duplexes with the inactive variant.

Of the analogs tested, only 8SG:A pairs (Table 1) were bound with similar affinity to OG:A pairs (below the ~3 pM limit of detection). The remaining analogs ranged in affinity, with equilibrium dissociation constants ranging from 20 pM to 600 pM (Figure Figure55). The presence of the 8-oxo-group correlates with the next highest affinity group of substrate analogs, 8OI:A, 7MOG:A, and 8OA:A, which all exhibit an approximately 10-fold reduced binding affinity (Kd’s of 20–40 pM) by MutY compared to OG:A. The influence of the oxo-group for high affinity binding is not readily explained with the MutY-lesion structures since there are no direct contacts to the 8-oxo group (Figure Figure22D). In contrast, the measured decreases in affinity with these three 8-oxo-containing analogs correlate with the loss of late-stage OG interactions observed in the MutY-lesion structures. The high affinity for analogs retaining the 8-oxo group suggests that it plays an important role in “locking” MutY into a high affinity conformation on the lesion mispair. The remaining analogs exhibit further reduced binding (another ~10-fold) ranging from 100–600 pM in the following order of Kd: 8BG:A < G:A < 1MOG:A < 8AG:A < 9ZG:A. Notably, 1MOG retains the 8-oxo group yet is much more reduced in affinity relative to the other 8-oxo-containing analogs. This additional erosion in affinity suggests that the methyl group at N1 may prevent full engagement in the OG-binding site, as well as remove an important H-bonding contact in the MutY–lesion complex. Thus, these results point to the importance of both the Watson–Crick face and Hoogsteen face of OG for the exceptionally high affinity of MutY for its native substrate and suggest a recognition checkpoint which involves simultaneous recognition of both faces of OG.

Recognition and Repair in Living Bacterial Cells

MutY-mediated repair in a cellular setting was observed by transforming muty+ or muty– cell lines with a plasmid containing a single site-specific X:A pair that was created by ligation of a sticky-ended X:A-containing duplex (Duplex 2) into a plasmid fragment derived from pACYC177 (Figure Figure66A). Transformed populations of each cell line were allowed to grow and to replicate the substrate plasmid.1 The amplified plasmid pools from at least five transformations were isolated and analyzed by restriction digestion and sequencing to determine the distribution of base pairs that resulted at the lesion location (Figure Figure66B). In these assays, OG:A-containing plasmids are fully repaired to the correct G:C base pairs in the presence of MutY (routinely ~95%); G:A base pairs, while substrates for MutY in vitro, are not repaired significantly over the background. In the absence of MutY, the background levels of G:C and T:A base pairs that result at the location of an OG:A mispair (~35% G:C, ~65% T:A) reflect replication across both bases of the mispair, with the OG coding partially like T to result in less than 50% final G:C content.1 The coding properties of the substrate analogs are also reflected in the distribution of base pairs that are observed in the plasmids that are retrieved from the muty– cells (Figure Figure66). For instance, 8OA is nonmutagenic and codes like adenine;33 hence, the resulting mixture of roughly 1:1 A:T and T:A pairs observed in the sequencing traces reflects the unrepaired replication of both strands. 8SG was the only analog that displayed any amount of MutY-mediated repair, as evidenced by a change in the observed base pair distribution of muty+ and muty– cells; adenines opposite 8SG were repaired by MutY with a small but statistically significant (P < 0.02) decrease in efficiency compared to adenines opposite OG. For all other analogs, the types and percentages of base pairs observed at the original analog base pair site were the same whether the plasmids were retrieved from muty+ or muty– cell lines (Figure Figure66B), indicating no MutY-mediated repair for adenines opposite 8AG, 8BG, 8OA, 8OI, 9ZG, 1MOG, or 7MOG.

Figure 6
Determining the cellular repair of OG analogs. (A) E. coli cell-based assay of MutY-mediated repair. Substrate plasmids containing a single, site-specific X:A pair (X = OG or an analog thereof) are prepared by ligating a sticky-ended duplex containing ...

Correlation of Catalysis and Substrate Binding Affinity

In general, the analogs that retain high affinity for MutY also are processed the most efficiently (Figure Figure77). This is not completely unexpected insofar as enzymatic catalysis cannot occur in the absence of enzyme–substrate binding. Notable exceptions to the correlation between high affinity and efficient excision were observed. For instance, although MutY had similar substrate affinity for 8OI:A pairs compared to 7MOG:A and 8OA:A pairs, 8OI:A pairs were catalyzed 10-fold more efficiently than 7MOG:A pairs and 40-fold more efficiently than 8OA:A pairs. Likewise, the substrate affinity of MutY to 8AG:A, 8BG:A, 1MOG:A, 9ZG:A, and G:A pairs were all within error of each other, but catalytic rates with these substrates varied substantially, with the fastest reactions (with G:A substrates) catalyzed 20-fold faster than the slowest (1MOG:A pairs). Rates of adenine removal from G:A, 8BG:A, and 8OA:A pairs were within error of one another; however, MutY exhibited a much greater affinity for the 8OA:A substrate. Notably, MutY cleaved adenines from G:A mismatches more readily than all analog pairs but 7MOG, 8OI, and 8SG-containing pairs, which suggests that neither the strength of syn conformational preference nor retention of a G-like Watson–Crick face or a OG-like Hoogsteen face are absolutely required for substrate processing. In the case of G:A, the lack of the 8-oxo substituent may impede initial recognition and base pair disruption; however, the similar Watson–Crick faces of G and OG may permit proper engagement in the OG-binding pocket needed to support adenine cleavage, albeit at a reduced rate compared to OG:A bps.

Figure 7
Rate of adenine cleavage (k2) by MutY versus substrate binding affinity (Kd) for X:A substrate pairs, where X is an analog of the OG base. Note the log scale of the y axis, which obscures the vertical error bars in cases such as OG:A pairs. Error bars ...

A feature that arises from this comparison of affinity and catalysis is that MutY may be exhibiting unproductive binding with some of the analog pairs and that, effectively, not all binding events are equal in leading to and promoting catalysis. Furthermore, these results indicate that the OG base has long-distance effects on the engagement of the scissile adenine into the extrahelical pocket, confirming that OG recognition takes precedence and occurs prior to recognition of the cleaved base to prevent cleavage of adenines from inappropriate pairing contexts. For example, MutY affinity for G:A and 1MOG:A bps are similar, but the adenine glycosylase is considerably slower with 1MOG:A suggesting that the absence of the proper G-like NH donor in 1MOG alters the ability to optimally place the adenine for excision. Similarly, MutY binds 8OA:A pairs 6-fold more tightly than G:A bps but excises adenine 2-fold slower from 8OA:A pairs, again pointing to long-range communication between the OG binding site and adenine excision site. Furthermore, comparing binding and catalysis indicates that some defects in binding do not dramatically alter catalysis. This is most striking with 8OI:A where MutY affinity is reduced at least 10-fold compared to OG:A, but base excision catalysis is only reduced 2-fold, further illustrating that all binding interactions do not impinge equally on the in vitro kinetic parameters for catalysis.

Correlation of Catalysis with Product Release

Catalysis and product release are inherently related insofar as they are only distinct when k2 [dbl greater-than sign] k3. This was observed in the lack of a burst of product formation for those substrate analogs that displayed slower catalysis by MutY: G, 1MOG, 8AG, 8BG, 8OA, and 9ZG (Table 1). Only three analog-containing base pairs retained a burst of product formation: 7MOG:A, 8OI:A, and 8SG:A. Importantly, the extremely tight product binding displayed by MutY and other glycosylases is thought to be a mechanism for regulating the inherently cytotoxic AP-site product; from these data, it is clear that the presence of OG is the determining factor in the exceptionally tight product regulation of MutY rather than the presence of the AP site itself. Furthermore, there appeared to be no change that would alter the product release term without altering the efficiency of base excision, indicating very tight coupling of the presence of the OG base to the preceding catalytic steps. These results underscore the importance of an oxo-like functionality in the nonscissile base for high affinity for the substrate and product, and concomitantly efficient in vitro adenine excision, and slow-AP site product release.

Correlation of Cellular Repair with in Vitro Enzymatic Behavior

Despite the wide range of observed catalytic rates and binding affinities of the substrate analogs tested, we were surprised to find that we did not observe a similar broad spectrum in the levels of observed cellular repair. The most conservative analog, 8SG, displayed a mild reduction in cellular repair in spite of showing no statistically significant difference in substrate binding, catalysis, or product release in vitro. This result suggests that even very subtle changes may have reverberating effects on the broader scale of cellular repair and underscores the idea that MutY exhibits tight control over opposite base recognition. Indeed, for 8SG to impact cellular repair in the face of retained kinetic behavior suggests that this control is extreme and includes factors as yet beyond our experimental reckoning.

All the other analogs eluded MutY completely in the cellular setting. Notably, this includes 8OI:A pairs, which showed a total ablation of MutY-mediated repair in cells despite being similar in catalytic rate to the repairable 8SG:A pairs. We attribute this startling loss of repair to an inability to effectively locate and intercept the 8OI:A in the cellular context. The potential origin of the defect leading to the absence of repair is hinted at by the 10-fold decrease in affinity observed in the EMSA experiment with E37S MutY and the 8OI:A-containing duplex. Catalytic efficiency would be expected to be a combination of catalysis and binding affinity (related to a specificity factor k2/Kd); however, in previous work we showed that large decreases in k2 do not result in significant levels of reduced cellular repair as long as OG:A-like affinity for the mismatch is retained. Specifically, we observed that OG:Z3 (Z3 = 3-deazaadenine) pairs are repaired in cells as efficiently as OG:A pairs despite a 200-fold reduction in k2.1 This correlation was also illustrated by analysis of specific mutations in catalytic residues in MutY that did not alter OG:A affinity.19 In comparison to the WT enzyme, the mutated enzymes in that work displayed much larger decreases in k2/Kd (200 to 300-fold) than the decrease in k2/Kd observed here between OG and 8OI (25-fold), and yet, unlike the complete elimination of cellular repair observed with the WT enzyme and 8OI:A pairs, those mutated enzymes retained a significant ability to mediate the repair of OG:A mispairs. The lack of a direct correlation with k2/Kd underscores that measured defects in k2 and Kd do not equally impact cellular repair. We suggest the differential impacts are due to magnifying binding defects in a cellular context. Indeed, location and engagement of substrate base pairs would be expected to be more difficult in a cellular context than on a 30-bp duplex in vitro due to the much higher concentration of highly similar normal T:A bps compared to OG:A mismatches. In addition, competition for DNA with other cellular proteins may also magnify the consequences of small differences in DNA affinity.34

The 2-Amino Group of OG Is a Key Intrahelical Recognition Feature

In comparing OG:A to T:A pairs, the presence of the 2-amino group in the major groove stands out as the major structural difference (Figure Figure11). We were initially surprised that the removal of the 2-amino group of OG in 8OI:A substrates resulted in only minor changes in in vitro glycosylase activity; however, considering the in vitro results alongside the absence of cellular repair implicates the 2-amino group recognition as a key element of early stages of detection of OG:A bps. Notably, an important role of the 8-oxo group of OG is favoring the syn conformation, and allowing for stable base pairing with A. Moreover, the OGsyn:Aanti base pairing conformation presents the 2-amino group of OG on the major groove side of the DNA helix. In prevalent G:C bps, the 2-amino group of G is localized in the minor groove. Thus, the presence of the 2-amino group in the major groove distinguishes OG:A bps from both T:A and G:C bps. Interestingly, recent structural work by Verdine and co-workers provided a glimpse of MutY interacting with an intrahelical OG:A bp by utilizing disulfide cross-linking with a mutant of the N-terminal domain of MutY that was incapable of extruding adenine.35 In this structure, the helix–hairpin–helix motif of the N-terminal domain is loosely engaged with the DNA phosphodiester backbone from the minor groove side, and the DNA is its canonical B-form. Comparing this structure to that of full-length MutY bound to lesion-containing DNA suggested that a loop region of the C-terminal domain may be in proximity to interact with OG:A from the major groove side.35 Due to the known role of the CTD in OG recognition,13,20,34,3638 and its required presence for cellular repair of OG:A mismatches,20,39 the idea that this CTD loop region could be important for OGsyn recognition via interactions with the 2-amino group is appealing. Indeed, these recognition features could involve direct hydrogen-bonding or steric interactions that aid in stalling MutY as it moves along DNA. Studies to address such ideas are presently in progress.

Lesion Verification Occurs via Multiple Checkpoints

The SAR revealed herein, taken together with previous structural and biochemical studies of MutY and related enzymes, provides insight into the key features of the OG:A mismatch that facilitate its efficient and selective recognition and repair by MutY. In the initial processive search process, we suggest that the projection of the 2-amino group of the OGsyn:Aanti mismatch provides the steric blockade that results in MutY pausing at the target bp. At this intrahelical recognition stage, the 8-oxo functional group likely participates in detection by MutY only indirectly. By creating a new hydrogen bond donor at N7, the oxo functional group facilitates a favorable OGsyn base pair with A and thereby forces the 2-amino group of OG to jut into the major groove. Once paused, MutY may also make additional contacts with the 2-amino group and further probe the OG:A bp by inducing a DNA bend at the lesion site. Bending and insertion of the MutY Tyr probe residue (Tyr88 in G. stearothermophilus MutY, see Figure Figure22D) from the minor groove facilitates OG:A base pair opening. The preferential disruption of OG:A bps may also be facilitated by steric clashes specific to the 8-oxo group that ensue as a result of the base pair buckling induced by the probe ligand insertion and DNA bending in a manner conceptually analogous to that used by Fpg in recognition of OG:C bps.40 Once the bp has been disrupted, the OG is placed within the OG pocket in the anti conformation, and the A is effectively inserted into the catalytic active site for cleavage. Full engagement of the OG within the OG site requires extensive interactions with multiple functional groups on both the Watson and Crick and Hoogsteen face of OG, but not directly with the 8-oxo group. The proper positioning of OG at this final stage prior to catalysis is communicated via proper “locking” of MutY and potentially via a hydrogen-bond network to the A cleavage site that positions catalytic residues appropriately for adenine excision. Only proper orientation of OG at this final stage, tightly clamped at both the Watson–Crick and Hoogsteen faces, allows for efficient adenine excision providing the final substrate verification.

Using this multistep confirmation process, MutY exerts strong opposite base control and avoids mutagenic action on adenines opposite other bases; by linking the binding of OG to catalysis at the distant adenine, the protein effectively uses OG as an activator for catalysis. Importantly, this insight suggests that at least part of the OG binding process overlaps temporally with adenine cleavage and thus is not entirely separable from catalysis.

Lesion Verification Process and MAP

The importance of key aspects of the “OG” revealed in the SAR highlight the structural motifs and specific amino acids of MutY that are involved in mediating interactions with the OG at various stages during the repair process. The critical role of the 2′-amino of OG revealed herein provides additional insight into the importance of the C-terminal domain and the region that is critical for this initial contact. There are over 100 MAP-associated missense variants that are localized throughout MUTYH, with many localizing to the C-terminal domain and other regions that are likely to interact with the OG lesion.5 Indeed, one of the most common MAP variants, Y165C, corresponds to the Tyr probe residue that intercalates 5′ to OG and plays an important role in the activity of MutY and MUTYH.6,20,34,37,39,41 In many cases, however, it is not obvious based on available structural and functional data why some variants would be dysfunctional and associated with MAP. Based on the high impact of modifications of OG described here, mutations that alter recognition and binding to OG would be expected to be particularly detrimental. Moreover, our work indicates that in vitro analysis alone may not accurately represent the dysfunction of variants that are involved in initial recognition and lesion detection. These results further underscore the importance of fully understanding the features required for efficient repair to predict the potential impact and disease risk of a given MAP variant.

Methods

General Methods and Materials

Commercially available enzymes were purchased from New England Biolabs and used according to the manufacturer’s protocol. [γ-32P]-ATP was purchased from PerkinElmer. Storage phosphor autoradiographs were scanned on a GE Healthcare Typhoon Trio phosphorimager. Quantitation and data analysis were performed using ImageQuant TL v5.2 (GE Healthcare Life Sciences), GraFit v5.0.2 (Erithacus Software), and Excel v14.7.3 (Microsoft). Electrostatic potential maps were produced using Gaussian09d (Gaussian, Inc.). Aqueous solutions were prepared using distilled, deionized water from a Milli-Q PF purification system. All other reagents were purchased from Sigma-Aldrich, ThermoFisher Scientific, Qiagen, or VWR.

DNA Substrate Preparation

For OG and the 8AG, 8BG, 8OA, and 9ZG analogs, precursor nucleoside amidites suitable for solid phase DNA synthesis were purchased from Glen Research. For 8SG, 7MOG, and 8OI, nucleoside amidites were synthesized as previously reported.4246 In the case of 1MOG, the nucleoside amidite was synthesized with substantial modifications from the literature (see Supporting Information). OG-containing and analog-containing DNA oligonucleotides were synthesized at the University of Utah core facility. DNA oligonucleotides containing only standard nucleobases were purchased from Integrated DNA Technologies. All DNA oligonucleotides were purified by HPLC on a Beckman Gold Nouveau system using a Dionex 100 ion exchange column. Deprotection of 8SG-containing oligonucleotides was performed as previously reported after HPLC purification.43 Masses of the single-stranded substrate oligonucleotides were confirmed by ESI-MS. A representative ESI-MS of a 1MOG-containing oligonucleotide is shown in the Supporting Information. Complementary oligonucleotide sequences were annealed overnight in annealing buffer (20 mM Tris-HCl, 10 mM EDTA, and 150 mM NaCl). Duplex 1 (Figure Figure44A) was used for all glycosylase and binding assays. Duplex 2 was used for ligation into the substrate plasmid for the cellular repair assay.

MutY Purification, Glycosylase, and Binding Assays

Wild type MutY and E37S MutY were overexpressed, purified on a GE Healthcare AKTA FPLC system, and quantitated as previously described.1,47 The fraction of binding-competent E37S MutY was determined by binding titrations of the enzyme with an OG:A substrate (20 nM). All enzyme concentrations were corrected for percent activity. Glycosylase assays to measure k2 and k3, as well as substrate binding assays to measure apparent Kd were performed as previously described.25,48 For reactions too fast to measure manually, a KinTek RQF-3 Rapid-Quench instrument was used.

Cellular Repair Assay

Measurements of MutY-initiated base excision repair were performed as previously described1 using a variety of substrate plasmids. Briefly, substrate plasmids were created by ligating Duplex 2 into a stretch of linear DNA from vector pACYC177 to produce pACYC(OG:A), pACYC(8BG:A), pACYC(8AG:A), pACYC(8SG:A), pACYC(G:A), pACYC(9ZG:A), pACYC(7MOG:A), pACYC(1MOG:A), pACYC(8OA:A), and pACYC(8OI:A). Each plasmid was transformed into muty+ or muty– bacterial cell lines, then amplified and isolated by midiprep (Promega Wizard). BmtI restriction analysis in conjunction with agarose gel electrophoresis gave percent repair to G:C pairs specifically; sequencing was used to confirm the restriction analyses and to identify other base pairs (i.e., T:A and A:T) that resulted at the initial lesion site.

Acknowledgments

The work was supported by grants from the National Institutes of Health CA067985 (S.S.D.) and NSF 1305548 (M.L.H.). A.H.M was a predoctoral trainee supported by T32-GM008799 from NIGMS-NIH. A.H.M. was also supported by the UCD Department of Chemistry and by GAANN and Corson-Dow Fellowships. M.L.H. is a Henry Dreyfus Teacher Scholar. The authors gratefully acknowledge the assistance of B. Hudson in creating the electrostatic potential maps in Gaussian. The contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIGMS or National Institutes of Health.

Glossary

Abbreviations

AP
apurinic
BER
base excision repair
bp
base pair
CTD
C-terminal domain
EDTA
ethylene diamine tetraacetate
EMSA
electrophoretic mobility shift assay
ESI-MS
electrospray ionization mass spectrometry
FPLC
fast protein liquid chromatography
HPLC
high-performance liquid chromatography
MAP
MUTYH-associated polyposis
OG
8-oxoguanine
PAGE
polyacrylamide gel electrophoresis
SAR
structure–activity relationship
Z3
3-deazaadenine
1MOG
1-methyl-8-oxoguanine
7MOG
7-methyl-8-oxoguanine
8AG
8-aminoguanine
8BG
8-bromoguanine
8OA
8-oxoadenine
8OI
8-oxoinosine
8SG
8-thioguanine
9ZG
9-deazaguanine

Supporting Information Available

Supporting Information Available

The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acschembio.7b00389.

  • Synthesis and characterization of 1MOG-containing nucleotide and oligonucleotides (PDF)

Author Contributions

Author Contributions

A.H.M., P.L.M., M.L.H., and S.S.D. designed the study. M.L.H. synthesized the 8SG and 7MeOG phosphoramidites. A.H.M. synthesized 1MOG- and 8OI-containing phosphoramidites. The purifications of DNA, enzyme, and cellular assays were performed by A.H.M., P.L.M., E.L.D., and C.M. A.H.M. and S.S.D. wrote the paper with input from the other authors.

Notes

The authors declare no competing financial interest.

Supplementary Material

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